Análise da cinética de remoção de lactato em atletas de canoagem slalom

The aim of this study was to verify the kinetics of lactate production and removal in slalom kayak athletes, K1 category, during official competition. Eight male athletes (22,6 ± 4,3 years) participated of the study. For the analysis of lactate, 25µL of capillary blood were collected. The kinetics of lactate removal was performed before the warm-up (Pre), just after the competitors exit from the river (Post 0'), 5 (Post 5'), and 20 (Post 20') minutes. The results demonstrated a significant increase in lactate concentrations (9.8 mmol/l, 9.4 mmol/l and 6.6 mmol/l) at 0', 5' and 20' post respectively, with values of P<0.01. The findings indicate that after 20 minutes the values of lactate reduced significantly (P<0.05) compared to Pre exercise, suggesting that the athletes would indicate good metabolic conditions for the second turn of the race.

Action of Brazilian propolis on hematological and serum biochemical parameters of Blue-fronted Amazons (Amazona aestiva, Linnaeus, 1758) in captivity

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Dosagem de metabólitos de glucocorticoides e progesterona em fezes de papagaio-verdadeiro (Amazona aestiva)

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Meiotic arrest of sheep oocytes using roscovitine under different medium compositions

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Luteal changes after treatment with sub-luteolytic doses of prostaglandin (cloprostenol sodium) in cattle

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Time course of the meiotic arrest in sheep cumulus-oocyte complexes treated with roscovitine

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Direito à soberania alimentar e ao patrimônio cultural: perspectivas para uma alimentação identitária

Pós-graduação em Direito - FCHS

Biopsias luteais em vacas Nelore

The present study describes a technique to obtain consecutive luteal samples by colpotomy. The animals received an epidural anesthesia and local anesthesia (vaginal vault) and after ten minutes the vaginal vault was incised with a scalpel blade and tissue was dissected to provide access to the pelvic cavity and to retract the ovary into the vagina. Then, a luteal biopsy was performed with a Yomann biopsy nipper. Signs indicative of pain and stress during the vaginal vault incision, traction of ovary or luteal biopsy were observed only in two collections. However, these signs were observed in ten collections during dissection of the vaginal wall and peritoneum. The occurrence of ataxia was observed in 26 collections and it was usually related to a longer duration of the procedure. Ataxia could be divided in light (15/26), moderate (6/26) and severe (5/26). The occurrence of ovarian adhesions ipsilateral to the incision was evaluated only in the initial four collections. Adhesions were present in 16 collections. The protocol described above provided a safe and efficient method to acquire luteal samples. The low incidence of adhesions allows the consecutive use of females without any interference in subsequent ovulations and collections.

Detecção imunoistoquímica de receptores de estrógeno e progesterona no endométrio de vacas Nelore (Bos taurus indicus) durante o anestro pós-parto

Twenty-four postpartum anestrous Nelore purebred cows were used in the study. Anestrous was determined based on the reproductive history which was confirmed in each cow based on plasma progesterone concentration and by transrectal ultrasonography. Endometrial biopsies were collected. The animals were separated into two groups according to maximum follicular diameter- Group 1: follicles <6mm (n=12) and Group 2: follicles >-6mm follicles (n=12). The immunohistochemistry technique was employed to evaluate the presence of estrogen and progesterone receptors in the uterine glandular epithelium and stroma. High counts of positive nuclei and high intensity of immunostain for estrogen and progesterone receptors in the glandular epithelium and stroma were observed in the two groups. However, the immunostain intensity of progesterone receptors in the glandular epithelium was higher in Group 2 compared to Group 1. When glandular epithelium and stroma were compared within each group, the relative number of estrogen receptors in the Group 1 was higher in the glandular epithelium compared to stroma and the immunostain intensity for the progesterone receptor in Group 2 was higher in the glandular epithelium compared to stroma. The results suggest that the mechanisms that control the expression of endomerial receptors in the anestrus are similar to the ones observed during the estrus cycle.

Um predador generalista na fronteira entre ecossistemas: interações tróficas e os processos ecossitêmicos bromelícolas

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

American Lives: A Reader [Preface & Introduction]

Memoirs are as varied as human emotion and experience, and those published in the distinguished American Lives Series run the gamut. Excerpted from this series (called “splendid” by Newsweek) and collected here for the first time, these dispatches from American lives take us from China during the Cultural Revolution to the streets of New York in the sixties to a cabin in the backwoods of Idaho. In prose as diverse as the stories they tell, writers such as Floyd Skloot, Ted Kooser, Peggy Shumaker, and Lee Martin, among many others, open windows to their own ordinary and extraordinary experiences. John Skoyles tells how, for his Uncle Fred, a particular “Hard Luck Suit” imparted misfortune. Brenda Serotte describes a Turkish grandmother who made her living reading palms, interpreting cups, and prescribing poultices for the community. In “Son of Mr. Green Jeans,” Dinty W. Moore views fatherhood through the lens of pop culture. Janet Sternburg’s Phantom Limb muses on the dilemmas of a child caring for a parent. Whether evoking moments of death or disease, in family or marriage, history, politics, religion, or culture, these glimpses into singular American lives come together in a richly textured, colorful patchwork quilt of American life.

Optimization of in situ derivatization SPME by experimental design for GC-MS multi-residue analysis of pharmaceutical drugs in wastewater

This paper presents the development of a procedure, which enables the analysis of nine pharmaceutical drugs in wastewater using gas chromatography-mass spectrometry (GC-MS) associated with solid-phase microextraction (SPME) for the sample preparation. Experimental design was applied to optimize the in situ derivatization and the SPME extraction conditions. Ethyl chloroformate (ECF) was employed as derivatizing agent and polydimethylsiloxane-divinylbenzene (PDMS-DVB) as the SPME fiber coating. A fractional factorial design was used to evaluate the main factors for the in situ derivatization and SPME extraction. Thereafter, a Doehlert matrix design was applied to find out the best experimental conditions. The method presented a linear range from 0.5 to 10 mu g/L, and the intraday and interday precision were lower than 16%. Applicability of the method was verified from real influent and effluent samples of a wastewater treatment plant, as well as from samples of an industry wastewater and a river.

Molecular features of sexual steroids on cartilage and bone

In Brazil, the increase in the reported cases of degenerative diseases of articular cartilage is 20% per year, meaning that 200,000 Brazilians develop degenerative joint diseases every year, which have a negative impact on bone mass. This study shows evidence that hormone production of sexual steroids (estrogens, progestogens, and androgens) have an influence on cartilage quality, as well as on bone mass. Therefore, this review aimed to analyze literature data on the molecular and genic action of sexual steroids on hyaline cartilage and bone physiology, as well as osteoarthritis interference on the quality of these structures.

Which patients are more likely to benefit from renal transplantation?

Background: We evaluated whether the advantages conferred by renal transplantation encompass all individuals or whether they favor more specific groups of patients. Methods: One thousand and fifty-eight patients on the transplant waiting list and 270 receiving renal transplant were studied. End points were the composite incidence of CV events and death. Patients were followed up from date of placement on the list until transplantation, CV event, or death (dialysis patients), or from the date of transplantation, CV event, return to dialysis, or death (transplant patients). Results: Younger patients with no comorbidities had a lower incidence of CV events and death independently of the treatment modality (log-rank = 0.0001). Renal transplantation was associated with better prognosis only in high-risk patients (p = 0.003). Conclusions: Age and comorbidities influenced the prevalence of CV complications and death independently of the treatment modality. A positive effect of renal transplantation was documented only in high-risk patients. These findings suggest that age and comorbidities should be considered indication for early transplantation even considering that, as a group, such patients have a shorter survival compared with low-risk individuals.

alpha,beta-Unsaturated Diazoketones as Platforms in the Asymmetric Synthesis of Hydroxylated Alkaloids. Total Synthesis of 1-Deoxy-8,8a-diepicastanospermine and 1,6-Dideoxyepicastanospermine and Formal Synthesis of Pumiliotoxin 251D

A versatile and concise approach for the stereoselective synthesis of mono-, di-, and trihydroxylated indolizidines is presented in four to six steps from Cbz-prolinal and a diazophosphonate. The key steps involved a Wolff rearrangement, followed by a stereoselective dihydroxylation/epoxidation reaction, from an alpha,beta-unsaturated diazoketone. The strategy also permits extension to the synthesis of many natural hydroxylated indolizidine alkaloids as demonstrated in the formal synthesis of pumiliotoxin 251D.