Subcellular localization of mammalian type II membrane proteins

Application of a computational membrane organization prediction pipeline, MemO, identified putative type II membrane proteins as proteins predicted to encode a single alpha-helical transmembrane domain (TMD) and no signal peptides. MemO was applied to RIKEN's mouse isoform protein set to identify 1436 non-overlapping genomic regions or transcriptional units (TUs), which encode exclusively type II membrane proteins. Proteins with overlapping predicted InterPro and TMDs were reviewed to discard false positive predictions resulting in a dataset comprised of 1831 transcripts in 1408 TUs. This dataset was used to develop a systematic protocol to document subcellular localization of type II membrane proteins. This approach combines mining of published literature to identify subcellular localization data and a high-throughput, polymerase chain reaction (PCR)-based approach to experimentally characterize subcellular localization. These approaches have provided localization data for 244 and 169 proteins. Type II membrane proteins are localized to all major organelle compartments; however, some biases were observed towards the early secretory pathway and punctate structures. Collectively, this study reports the subcellular localization of 26% of the defined dataset. All reported localization data are presented in the LOCATE database (http://www.locate.imb.uq.edu.au).

Words and intelligence II:essays in honor of Yorick Wilks

Yorick Wilks is a central figure in the fields of Natural Language Processing and Artificial Intelligence. His influence has extends to many areas of these fields and includes contributions to Machine Translation, word sense disambiguation, dialogue modeling and Information Extraction.This book celebrates the work of Yorick Wilks from the perspective of his peers. It consists of original chapters each of which analyses an aspect of his work and links it to current thinking in that area. His work has spanned over four decades but is shown to be pertinent to recent developments in language processing such as the Semantic Web.This volume forms a two-part set together with Words and Intelligence I, Selected Works by Yorick Wilks, by the same editors.

Part II of the Landlord and Tenant Act 1954:repeal or further reform? Part 1

Paper includes results of an empirical survey of legal professionals who specialise in property law and surveyors, both of whom are involved with commercial lease renewal work. The survey investigates whether there is any appetite amongst these professionals for abolishing the Landlord and Tenant Act 1954 Part II or alternatively for reform of the same. The results are analysed and recommendations made by the authors.

Part II of the Landlord and Tenant Act 1954:repeal or further reform? Part 2

See abstract for Part 1 of this paper.

Annotated record of the detailed examination of Mn deposits from the Snellius II - Cruise 4 stations in the vicinity of the island of Misool, Eastern Indonesia

The chemical analyses of ferromanganese encrustations found on the seabed west of Misool, eastern Indonesia, indicate that these deposits formed in a way different from that of world-wide occurring manganese nodules. Ferromanganese coated pebbles and fragments that were found in the deeper parts of the study area probably originate from nearby ridges. The ferromanganese crust on the upper part of a dolomite fragment of ?30 kg is likely to be formed by hydrogenous processes, whereas that from the lower part seems to be formed by diagenetic processes mainly. These assumptions are supported by pore-water data from two box cores taken in the same area. The manganese and iron profiles versus depth in these cores indicate a high flux of these metals to the uppermost sediment layer, and possibly into the overlying bottom water. Factor analysis for the principal components of the microprobe analytical results of the mainly hydrogenous ferromanganese crust demonstrates a strong correlation of manganese with the trace metals, of iron with phosphorus and an antipathetic relationship between iron and manganese. Similar results have also been reported for abyssal manganese nodules in the world oceans. Factor analysis for the principal components of the analytical data obtained for the diagenetic ferromanganese crust results in a clear dolomite (Ca/Mg) dilution factor only.

Annotated record of the detailed examination of Mn deposits from the CLIMAX II (SCAN) Expedition stations aboard R/V Argo in the central North Pacific

A 0.25 m US Naval Electronics Lab box corer was used to take replicate samples from an oligotrophic bottom under the North Pacific Central Water Mass (approx. 28 degrees N, 155 degrees W). The bottom is a red clay with manganese nodules at a depth of 5500-5800 m. Macrofaunal density ranges from 84 to 160 individuals per m super(2) and is therefore much the same as in Northwest Atlantic Gyre waters. Of the macrofaunal taxa, polychaetes dominate (55 per cent), followed by tanaids (18 per cent), bivalves (7 per cent), and isopods (6 per cent). Meiofaunal taxa were only partially retained by the 297 micrometer screen used in washing. Even then, they are 1.5-3.9 times as abundant as the microfaunal taxa, with nematodes being numerically dominant by far. Foraminifera seem to comprise an important portion of the community, but could not be assessed accurately because of the inability to discriminate living and dead tests. Remains of what are probably xenophyophoridans are also very important, but offer the same problem. Faunal diversity is extremely high, with deposit feeders comprising the overwhelming majority. Most spp are rare, being encountered only once. The distributions of only 3 spp show any significant deviation from randomness. The polychaete fauna from box cores collected from 90 m to the north was not significantly different from that of the principal study locality. Concordance appeared at several taxonomic levels, from spp through microfaunal/ meiofaunal relationships. As a result, the variation in total animal abundance shows aggregation among cores. The authors discuss Sokolova's concept of a deep-sea oligotrophic zone dominated by suspension feeders, and reconcile it with our present findings. The high diversity of the fauna combined with the low food level contradict theories that relate diversity directly with productivity.

Annotated record of the detailed examination of Mn deposits in the Atlantic Ocean from cruises from R/V Atlantis II, R/V Knorr, R/V Melvin and R/V James M. Gilliss in 1980-1981

This report is Volume 3 of Descriptions of WHOI rock dredge samples. This series represents a major effort to catalog the rock dredge samples in the WHOI Sea Floor samples collection, and to disseminate this information throughout the scientific community. Volume 3 contains sample descriptions and station data for the dredge stations from five cruises during the period September 1978 through December 1980. The material in this and subsequent volumes of rock descriptions was largely prepared onboard ship by the participating scientists. Volume 3 was printed prior to volumes 1 and 2 because of the excellent documentation of the samples represented in this volume.

Évaluation de la validité de l'ophtalmoscope confocal à balayage laser (HRT II) dans le dépistage de la neuropathie glaucomateuse chez des populations à haut risque : une étude pilote

Mémoire numérisé par la Direction des bibliothèques de l'Université de Montréal.

(Table II, page 668-669) Distribution of elements between minerals soluble in 1M Hydroxylamine hydrochloride (Reducible); in 1m HCL (Acid-soluble) and in the insoluble residue of manganese nodules

Attempts to classify pelagic sediments have been based either on appearance and composition, or on the ultimate origin of the components. In particular it appears feasible to distinguish minerals which crystallized in sea-water from those which formed in magmas, in hydrothermal solution, or by weathering under acidic conditions. It is the case of iron and manganese oxide mineral aggregates which constitute one of the major types of rock encountered on the ocean floor; according to Menard (unpublished) about 10% of the pelagic area of the Pacific is covered by such nodules. The nodules consist of intimately intergrown crystallites of different minerals among those identified, besides detrital minerals and organic matter, are opal, goethite, rutile, anatase, barite, nontronite, and at least three manganese oxide minerals of major importance. Arrhenius and Korkisch (1959) have attempted to separate from each other the different minerals constituting the nodules, in order to establish the details of their structure and the localization of the heavy metal ions. The results demonstrate (Table II) that copper and nickel are concentrated in the manganese oxide phases concentrated in the reducible fraction. Cobalt, part of the nickel and most of the chromium are distributed between these and the acid-soluble group of the non-manganese minerals, dominated by goethite and disordered FeOOH.

Annotated record of the detailed examination of Mn deposits from PR II, CORPUS 4 Cruise, R/V Atlantic Twin off the coast of Puerto Rico

The dredges described in this report were taken on the PR II, CORPUS 4 Expedition in January 1969 by the USGS Woods Hole Coastal and Marine Science Center from the R/V Atlantic Twin. Dredges recovered and are available at USGS Woods Hole Coastal and Marine Science Center for sampling and study.

Annotated record of the detailed examination of Mn deposits from R/V Tokaidaigaku Maru II Cruise GDP-1 stations

The present paper, deals with the results of the bottom sampling. Sampling devices were a gravity corer for heat flow measurement and dredges of bucket type.

Fadrique Álvarez de Toledo, II Duque de Alba y su inventario de libros (1531): Una biblioteca patrimonial

Fadrique Álvarez de Toledo, que nació hacia 1460 y murió en 1531, lidera en Castilla el linaje de la casa de Alba en el tránsito de la Edad Media al Renacimiento. Primo carnal de Fernando el Católico, y colaborador íntimo de los Reyes, desarrolla una amplia labor de mecenazgo artístico y literario, de la cual son ejemplos relevantes los apoyos a poetas como Juan del Encina, Juan Boscán o Garcilaso de la Vega. A pesar de esta actividad, la crítica no se ha ocupado con detalle de este personaje por haberle eclipsado un tanto su nieto, el Gran Duque de Alba. Este artículo reproduce el inventario de la biblioteca de Don Fadrique (formado por los libros que fueron catalogados a su muerte), inédito hasta ahora y que nos muestra una rica biblioteca patrimonial de ciento ochenta y seis obras. Entre los libros religiosos encontramos biblias, tratados de oración y libros de horas; se hallan también algunos títulos indispensables de las tradiciones franciscana y dominica, que nos muestran a un personaje interesado en las nuevas corrientes devocionales de finales del siglo XV, en el escolasticismo y en las mujeres santas y visionarias del Medievo.

El dinero en la historia del pensamiento económico: la teoría monetaria postkeynesiana y su confrontación con la ortodoxia

Las disputas en torno a determinados aspectos del dinero, como su neutralidad y el carácter endógeno o exógeno de la oferta monetaria, han sido permanentes entre las distintas escuelas de pensamiento y autores, estando su origen, probablemente, en la época de desarrollo del pensamiento escolástico. En este artículo pretendemos, en primer lugar, realizar un recorrido cronológico e histórico sobre el tratamiento científico económico del dinero, para, en segundo lugar, poner sobre la mesa la macroeconomía ortodoxa a la que han dado lugar las interpretaciones al respecto, así como los enfoques alternativos frente a este pensamiento dominante. Finalmente, intentamos poner en valor los desarrollos monetarios post-keynesianos, integrados en lo que denominan “Economía Monetaria de Producción”, confrontándolos con la llamada Nueva Síntesis Neoclásica.

Synthesis of 2-Alkynoates by Palladium(II)-Catalyzed Oxidative Carbonylation of Terminal Alkynes and Alcohols

A homogeneous PdII catalyst, utilizing a simple and inexpensive amine ligand (TMEDA), allows 2-alkynoates to be prepared in high yields by an oxidative carbonylation of terminal alkynes and alcohols. The catalyst system overcomes many of the limitations of previous palladium carbonylation catalysts. It has an increased substrate scope, avoids large excesses of alcohol substrate and uses a desirable solvent. The catalyst employs oxygen as the terminal oxidant and can be operated under safer gas mixtures.