Accurate sodium bisulfite sequencing in plants.

DNA cytosine methylation is a conserved epigenetic modification frequently correlating with transcriptional silencing in a wide variety of eukaryotic organisms. Sodium bisulfite treatment of DNA converts unmethylated cytosine to uracil, while 5-methylated cytosine is protected. We describe techniques that ensure reliable sequencing data following sodium bisulfite conversion and to avoid common pitfalls such as amplification of unconverted DNA and inclusion of sibling clones.

Meiosis in flowering plants and other green organisms.

Sexual eukaryotes generate gametes using a specialized cell division called meiosis that serves both to halve the number of chromosomes and to reshuffle genetic variation present in the parent. The nature and mechanism of the meiotic cell division in plants and its effect on genetic variation are reviewed here. As flowers are the site of meiosis and fertilization in angiosperms, meiotic control will be considered within this developmental context. Finally, we review what is known about the control of meiosis in green algae and non-flowering land plants and discuss evolutionary transitions relating to meiosis that have occurred in the lineages giving rise to the angiosperms.

Período crítico de protección contra plagas del follaje y frutos del cultivo de la sandia (Citrullus vulgaris sugar baby) de exportación

Se hizo un experimento para determinar el efecto de periodos de protección de plagas del follajes y frutos sobre el rendimiento y la ganancia neta en el cultivo de sandía para exportación. el experimento fue ubicado entre Nagarote y la paz centro, realizándose en los meses de febrero a abril de 1988, bajo riego. Para la protección del cultivo de las plagas se utilizó el insecticida prfenofos más riopcord (tambo 880 ecu). La dinámica poblacional mostró que las plagas que provocaron daños directo a los frutos como larvas de spodoptera spp. (lepidóptera: noctuidae) y heliothis ssp. (Lepidóptera: noctuidae), se acentuaron desde los 40 días después de la emergencia (DDE) de las plantas hasta el final de la cosecha. Las plagas que afectaron principalmente los follajes como lyriomiza spp. (Díptera: agromizidae) y aphis spp. Homóptera: aphididae) fueron más constante pero que a partir de los 38 DDE aumentaron sus poblaciones y provocaron un daño severo. El tratamiento que fue protegido desde la emergencia de la planta hasta la cosecha ocupo el primer lugar en fruto/mz, pero sin diferencias significativas entre los periodo de protección a excepción del testigo (sin protección). Así también se colocó en el primer lugar en el número de cajas con frutos exportables por su tamaño/mz, pero sin diferencia significativas con los periodos protegidos desde los 19 y 43 DDE hasta la cosecha. El periodo protegido en ninguna fase de cultivo y el periodo protegido desde la emergencia hasta los 43 DDE ocuparon el primer lugar en fruto no exportable por su tamaño demasiado pequeño. El tratamiento protegido desde los 19 DDE hasta la cosecha presento la mayor ganancia neta. Se presentó deformación de frutos entre 70 y80 %, no presentándose efecto de periodo de protección.

Income risk of EU coal-fired power plants after Kyoto

Coal-fired power plants may enjoy a significant advantage relative to gas plants in terms of cheaper fuel cost. Still, this advantage may erode or even turn into disadvantage depending on CO2 emission allowance price. This price will presumably rise in both the Kyoto Protocol commitment period (2008-2012) and the first post-Kyoto years. Thus, in a carbon-constrained environment, coal plants face financial risks arising in their profit margins, which in turn hinge on their so-called "clean dark spread". These risks are further reinforced when the price of the output electricity is determined by natural gas-fired plants' marginal costs, which differ from coal plants' costs. We aim to assess the risks in coal plants' margins. We adopt parameter values estimated from empirical data. These in turn are derived from natural gas and electricity markets alongside the EU ETS market where emission allowances are traded. Monte Carlo simulation allows to compute the expected value and risk profile of coal-based electricity generation. We focus on the clean dark spread in both time periods under different future scenarios in the allowance market. Specifically, bottom 5% and 10% percentiles are derived. According to our results, certain future paths of the allowance price may impose significant risks on the clean dark spread obtained by coal plants.

Depletion of the heaviest stable N isotope is associated with NH4 +/NH3 toxicity in NH4 +-fed plants

Background: In plants, nitrate (NO(3)(-)) nutrition gives rise to a natural N isotopic signature (delta(15)N), which correlates with the delta(15)N of the N source. However, little is known about the relationship between the delta(15)N of the N source and the (14)N/(15)N fractionation in plants under ammonium (NH(4)(+)) nutrition. When NH(4)(+) is the major N source, the two forms, NH(4)(+) and NH(3), are present in the nutrient solution. There is a 1.025 thermodynamic isotope effect between NH(3) (g) and NH(4)(+)(aq) which drives to a different delta(15)N. Nine plant species with different NH(4)(+)-sensitivities were cultured hydroponically with NO(3)(-) or NH(4)(+) as the sole N sources, and plant growth and delta(15)N were determined. Short-term NH(4)(+)/NH(3) uptake experiments at pH 6.0 and 9.0 (which favours NH(3) form) were carried out in order to support and substantiate our hypothesis. N source fractionation throughout the whole plant was interpreted on the basis of the relative transport of NH(4)(+) and NH(3). -- Results: Several NO(3)(-)-fed plants were consistently enriched in (15)N, whereas plants under NH(4)(+) nutrition were depleted of (15)N. It was shown that more sensitive plants to NH(4)(+) toxicity were the most depleted in (15)N. In parallel, N-deficient pea and spinach plants fed with (15)NH(4)(+) showed an increased level of NH(3) uptake at alkaline pH that was related to the (15)N depletion of the plant. Tolerant to NH(4)(+) pea plants or sensitive spinach plants showed similar trend on (15)N depletion while slight differences in the time kinetics were observed during the initial stages. The use of RbNO(3) as control discarded that the differences observed arise from pH detrimental effects. -- Conclusions: This article proposes that the negative values of delta(15)N in NH(4)(+)-fed plants are originated from NH(3) uptake by plants. Moreover, this depletion of the heavier N isotope is proportional to the NH(4)(+)/NH(3) toxicity in plants species. Therefore, we hypothesise that the low affinity transport system for NH(4)(+) may have two components: one that transports N in the molecular form and is associated with fractionation and another that transports N in the ionic form and is not associated with fractionation.

The Fossil higher plants from the Canal Zone

Fossil flora described in the present report is too limited for purposes of exact correlation, which may be expected to be settled by the marine faunas present at most horizons in the Isthmian region. Accompanying table of distribution will show that from the oldest (Hohio) to the youngest (Gatun) plant-bearing formations there is no observable difference in floral facies. This so-called Oligocence series of formations does not represent any great interval of time. (39 page document)

The Effects of Grass Carp on Aquatic Plants, Plankton and Benthos in Ponds

The effects of the grass carp (Ctenopharyngodon idella Val.)on aquatic plant biomass, water quality, phytoplankton, chlorophyll a, zooplankton and benthic fauna were investigated between May and September 2000 in earthen ponds at Cifteler- Sakaryabasi Aquaculture and Research Station. (PDF has 8 pages)

Carfentrazone-ethyl Pond Dissipation and Efficacy on Floating Plants

Carfentrazone-ethyl (CE) is a reduced risk herbicide that is currently being evaluated for the control of aquatic weeds. Greenhouse trials were conducted to determine efficacy of CE on water hyacinth ( Eichhornia crassipes (Mart.) Solms- Laub.), water lettuce ( Pistia stratiotes L.), salvinia ( Salvinia minima Baker) and landoltia (Landoltia punctata (G. Mey.) Les & D. J. Crawford ) . CE controlled water lettuce, water hyacinth and salvinia at rates less than the maximum proposed use rate of 224 g ha -1 . Water lettuce was the most susceptible to CE with an EC 90 of 26.9 and 33.0 g ha -1 in two separate trials. Water hyacinth EC 90 values were calculated to be 86.2 to 116.3 g ha -1 , and salvinia had a similar susceptibility to water hyacinth with an EC 90 of 79.1 g ha -1 . Landoltia was not adequately controlled at the rates evaluated. In addition, CE was applied to one-half of a 0.08 ha pond located in North Central, Florida to determine dissipation rates in water and hydrosoil when applied at an equivalent rate of 224 g ha -1 . The half-life of CE plus the primary metabolite, CE-chloropropionic acid, was calculated to be 83.0 h from the whole pond, and no residues were detected in water above the limit of quantification (5 μg L -1 ) 168 h after treatment. CE dissipated rapidly from the water column, did not occur in the sediment above the levels of quantification, and in greenhouse studies effectively controlled three species of aquatic weeds at relatively low rates.(PDF contains 6 pages.)

Factors to consider when using native biological control organisms to manage exotic plants

Biological control of exotic plant populations with native organisms appears to be increasing, even though its success to date has been limited. Although many researchers and managers feel that native organisms are easier to use and present less risk to the environment this may not be true. Developing a successful management program with a native insect is dependent on a number of critical factors that need to be considered. Information is needed on the feeding preference of the agent, agent effectiveness, environmental regulation of the agent, unique requirements of the agent, population maintenance of the agent, and time to desired impact. By understanding these factors, researchers and managers can develop a detailed protocol for using the native biological control agent for a specific target plant. . We found E. lecontei in 14 waterbodies, most of which were in eastern Washington. Only one lake with weevils was located in western Washington. Weevils were associated with both Eurasian ( Myriophyllum spicatum L.) and northern watermilfoil ( M. sibiricum K.). Waterbodies with E. lecontei had significantly higher ( P < 0.05) pH (8.7 ± 0.2) (mean ± 2SE), specific conductance (0.3 ± 0.08 mS cm -1 ) and total alkalinity (132.4 ± 30.8 mg CaCO 3 L -1 ). We also found that weevil presence was related to surface water temperature and waterbody location ( = 24.3, P ≤ 0.001) and of all the models tested, this model provided the best fit (Hosmer- Lemeshow goodness-of-fit = 4.0, P = 0.9). Our results suggest that in Washington State E. lecontei occurs primarily in eastern Washington in waterbodies with pH ≥ 8.2 and specific conductance ≥ 0.2 mS cm -1 . Furthermore, weevil distribution appears to be correlated with waterbody location (eastern versus western Washington) and surface water temperature.

Common Coastal Plants in Florida: a guide to planting and maintenance

(PDF has 125 pages.)