Curcumin modulates the immune response associated with LPS-induced periodontal disease in rats

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Sequential IL-23 and IL-17 and increased Mmp8 and Mmp14 expression characterize the progression of an experimental model of periodontal disease in type 1 diabetes

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Lobe Variation Effects of Experimental Diabetes and Insulin Replacement on Rat Prostate

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Cellular changes in the prostatic stroma of glucocorticoid-treated rats

Glucocorticoid hormones (GCs) have been widely used for the treatment of prostate cancer because of their inhibitory property against tumour growth. However, their mechanism of action in the prostate has received little attention. Excess GCs can lead to peripheral insulin resistance resulting in hyperglycaemia and hyperinsulinaemia. Insulin plays an important role as a cellular stimulant and high levels are related to low levels of androgens. Our objective has been to describe the effects of insulin resistance induced by dexamethasone treatment on the morphology of rat ventral prostate. Mate adult Wistar rats received daily intraperitoneal injections of dexamethasone or saline for five consecutive days after which the rats were killed and the ventral prostate was removed, weighed and prepared for conventional and transmission electron microscopy (TEM). Dexamethasone treatment resulted in atrophy and decreased proliferative activity of prostatic epithelial cells. TEM analysis revealed changes in the epithelium-stroma interface, with some interruptions in the basement membrane. Fibroblasts showed a secretory phenotype with dilated endoplasmic reticulum. Smooth muscle cells exhibited a contractile pattern with 50% atrophy, an irregular membrane and twisted nuclei. Mitochondrial alterations, such as enlarged size and high electron density in the mitochondrial matrix, were also detected in smooth muscle cells. Insulin resistance induced by dexamethasone is thus associated with epithelial atrophy similar to that described for diabetic rats. However, GCs are responsible for morphological changes in the stromal cell population suggesting the activation of fibroblasts and atrophy of the smooth muscle cells.

Short-term stromal alterations in the rat ventral prostate following alloxan-induced diabetes and the influence of insulin replacement

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Tratamento crônico com extrato alcoólico de Pterogyne nitens não melhora parâmetros clássicos do diabetes experimental

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Estudo do extrato de Bauhinia forficata L. sobre o diabete em ratas prenhes

The objective of this paper was to study the aqueous extract of Bauhinia forficata L. (pata-de-vaca) effects on streptozotocin-induced diabete pregnant rats. We used Wistar pregnant rats, dividided in 3 experimental groups: control (C, n=7), non-treated diabetics (DNT, n=7) and streptozotocin induced-diabetics treated with an aqueous extract of pata-de-vaca leaves, 200 mg/kg dose (DT, n=7). The animals received the extract through a gastric tube (gavage). The blood glucose level were verified on day 0, 5, 14 and 20 of pregnancy. During pregnancy, the daily mean water intake, food intake and average maternal weight gains of rats were measured. The results demonstrated that plant extract reduced the postimplantation loss porcentage, increasing the number of live fetuses likely to the control group. We found increased food and water intake of the DT and DNT pregnant rats compared to control due to hyperglycemic state. We also observed average maternal weight gains was likely to the DT and control groups on different pregnant periods, suggesting treatment with the plant contributed for the rat weight gains. The blood glucose level of dams did not present significative differences between DT and DNT groups. Thus, the B. forficata aqueous extract, 200 mg/kg dose, did not present hypoglycemic effect on streptozotocin-induced diabete pregnant rats. Nevertheless, the results suggest that DT pregnant rats were kept safe the for B. forficata aqueous extract, allowing at term pregnant occurence.

Estudo do extrato de Ginkgo biloba na prevenção de malformações em recémnascidos de ratas diabéticas

The pregnant women presenting Diabetes mellitus develop metabolic alterations, that may cause damage to the fetal well-being and provoke anomalies and/or malformations. The antioxidant treatment has improved the embryonic development from streptozotocin diabetic rats. Several studies have shown that a Gingko biloba extract presents antioxidant effects and, in the present study, one of the G. biloba extract formulations was used (EGb761) - Tebonin (200 mg/Kg/day), given to the diabetic pregnant female rats. The aim was to evaluate the effect of the EGb761 treatment on the of anomalies and/ or malformations incidence of the offspring. Diabetes was induced in female rats using streptozotocin in a dose of 40 mg/kg. The rats were mated, and the pregnant animals were divided in two groups: Control (water) and experimental (G. biloba). At day 21 of pregnancy, the rats were killed, and their fetuses were analyzed and processed for anomalies and/or malformations incidence. The results demonstrated that control and experimental groups presented no external anomalies and malformations; increased incidence of skeletal anomalies and of visceral malformations, and lower rate of visceral anomalies and skeletal malformations. These data confirm no statistical difference and, therefore, EGb761 treatment did not cause changes. Thus, a dose of 200 mg/Kg/day of a Gingko biloba extract given during the pregnancy rat was ineffective in the prevention of the anomalies and/or malformations related to the diabetes.

Avaliação da população de neurônios mioentéricos NADPH-diaforase positivos do corpo do estômago de ratos com diabetes crônico induzido pela estreptozootocina

The aim of this study was to analyze the morphoquantitative features of the nitrergic myenteric neurons from the body of the stomach of diabetic rats. The body of the stomach of five normoglicemic rats and of five diabetic rats were prepared as whole-mounts stained by the histochemical technique of NADPH-diaphorase. Decreased body weight and increased daily ingestion of water, fast glicemia and glycated hemoglobin were verified in diabetic animals. According to the data obtained, significant difference in the density of nitrergic neurons was not observed between the two groups, but the areas of the neuronal cell body profiles in the diabetic rats were significantly larger. Results showed that the streptozotocin that induced diabetes does not accelerate the death of the nitrergic neurons, but increases the expression of these cells.

Efeito do extrato aquoso de folhas de Polymnia sonchifolia (yacon) em ratas diabéticas

Polymnia sonchifolia is a medicinal plant used as hypoglycemic agent and other ends. The aim was to evaluate the yacon effect on diabetes in Wistar female rats, which was divided in 4 groups: non-diabetic (n=24) given (gavage) 32.4 mg kg -1 of an aqueous extract of plant (treated group) or vehicle (non-treated group), and one diabetic group (n=24) with similar treatment compared to non-diabetic rats. Diabetes was induced using streptozotocin (i.v. route, 40 mg kg -1). Glycemia was monitored after rodent adaptation period, 15 days after diabetes induction, and each 10 days after yacon/vehicle in early treatment. During experiment period, body weights, food and water intake were daily measured. The rats (non-diabetic or diabetic) given yacon extract treatment presented no significant difference related with glycemia, body weight gain, and food and water intake. Thus, further studies such increase of rat number in agreement with different glycemic levels and increase of the plant extract dose are necessary.

Avaliação morfométrica dos hepatócitos de ratos diabéticos tratados com neem (azadirachta indica a. juss) e estreptozootocina 6 ch

The effects of extracts from Azadirachta indica A. Juss and streptozotocin, in extreme high dilutions in dinamized systems, on hepatocyte morphometry of rats with diabetes mellitus induced by streptozotocin were evaluated. Some parameters presented significant differences among the experimental groups, but the effect of treatments to make better or worse the consequences of diabetes mellitus on liver was not detected using only the hepatocyte morphometry. The morphometric analysis evidenced that the white control group presented the highest mean values for nucleus and cytoplasm parameters, with the exception of the nuclear shape factor. However, this situation did not imply the diabetes mellitus was the cause of drastic reduction in these values, since the literature describes lower measurements for rats from the control group (non-diabetic rats).

Efeito da inflamação do tornozelo sobre as características histológicas, a expressão gênica e níveis da creatina cinase nos músculos sóleo e tibial anterior de ratos diabéticos

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Influência do estado diabético na doença periodontal induzida em ratos. Análise bioquímica, microscópica, radiográfica e dos níveis de mieloperoxidade

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Influência do meio hiperglicêmico intrauterino em diferentes fases de vida dos descendentes de ratas

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Avaliação do estresse oxidativo no sangue e na placenta de ratas com diabete de intensidade moderada

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)