Using new scanning electron microscopy (SEM) approaches to elucidate bacterial biofilm architecture

Healthcare-associated infections (HAI) are a major public health problem being Klebsiella pneumoniae and nontuberculous mycobacteria, both with high antibiotic resistance rates, among their etiological agent. Since biofilme assembly is pointed as one of the mechanisms involved in emergence of antibiotic resistance understanding bacteria organization within the biofilm and the identification of differences between planktonic and sessile forms of bacteria will be a step forward to fight HAI. In the present work we used SEM as a tool to characterize the internal structure of biofilm assembled on different surfaces. For SEM analysis, biofilms were allowed to form either on six-well cell culture plates, silicon or metallic disks placed inside the wells for different incubation periods at 37 °C. The biofilm assembled on the cell culture dish was for both secondary and backscattered electron analysis as described before. Biofilms assembled on silicon disks instead of being sectioned were prepared as metallographic samples, by grinding with grit SIC paper and polishing with diamond particles. Samples were cleaned (70% ethanol), dried with hot air, further coated and analysed. A preliminary study using FIB-SEM has been performed to access the ultrastructure of biofilms assembled on metallic surfaces. The results obtained showed that the same bacteria assembled biofilms with different ratios of biomass and extracellular matrix depending on the surface. SEM performed on thin sections of biofilms is a powerful tool to elucidate biofilm structure allowing the quantification of the major components. FIB-SEM is also a promising tool in this field.

Classic and new approaches to evaluate risk assessment for public health related to exposure to Halogenated Polycyclic Aromatic Hydrocarbons present in ludic waters

Risk assessment for public health related to exposure to Halogenated Polycyclic Aromatic Hydrocarbons present in ludic waters

Possible Approaches to Commercializing Applied University Research: a Russian Case Study

This paper discusses the environment around universities in Russia and possible approaches to analyzing and choosing the method for applied research results commercialization as well as selecting promising applied research areas in that environment. Conceptual foundations for decision making during the commercialization and roadmap/action plan creation processes are outlined. These can be useful to both universities for planning their activities aswell as for organizations that plan to cooperate with universities or that are interested in university generated research. This being said, obtained models and used evaluation parameters may be unique and may depend upon the particular project, university, region, and personal preferences of decision makers. Thus, consideration of these parameters and characteristics only has merit when making decisions in the dynamics of change of these parameters. For this purpose statistical information is needed that characterizes the competencies of the research organization (university) inquestion, needs of partner organizations, governmental and societal requirements, and science and technology prospects. After determining the promising research areas it’s time to look at particular projects, which in turn are also characterized by various parameters dependent upon their objectives. Considering the values of these parameters in their dynamics allows control of project parameters in the course of its execution. This in turn allows prediction of negative situations and alleviation of such by setting the target values of parameters and using best practices and standardization of management processes to achieve those values.

New approaches to the preservation of contaminants in water samples /

Jan. 1979.

Beneath the bottom line : agricultural approaches to reduce agrichemical contamination of groundwater : summary.

"OTA-F-417"--P. [4] of cover.

New approaches to CETA training : an overview of the Title III National Program for Selected Population Segments.

"Prepared for the Employment and Training Administration, U.S. Department of Labor under research and development contract no. 20-25-77-20."

Trouble shooting electronic equipment; an empirical approach to the identification of certain requirements of a maintenance occupation. A report of an aspect of a research project under Air Force Contract no.33 (038)-13236, Project no. 507-007-0001,

Bibliography: p. 100-102.

Innovative approaches to teaching technical communication /

Includes bibliographical references and index.

Legal and institutional approaches to water quality management planning and implementation /

Includes bibliographies.

A methodology for evaluating alternative technical and information management approaches to privacy requirements /

Prepared for the Institute for Computer Sciences and Technology, National Bureau of Standards.

Some statistical and mathematical approaches to meteorology : final report /

Final Report under Contract No.

Approaches to the calculation of limitations on nuclear detonations for peaceful purposes.

Paper presented as part of the National Center for Radiological Health's Seminar Program on Mar. 8, 1968, in Rockville, Md.