Sir3-dependent assembly of supramolecular chromatin structures in vitro

Baculovirus-expressed recombinant Sir3p (rSir3p) has been purified to near homogeneity, and its binding to naked DNA, mononucleosomes, and nucleosomal arrays has been characterized in vitro. At stoichiometric levels rSir3p interacts with intact nucleosomal arrays, mononucleosomes, and naked DNA, as evidenced by formation of supershifted species on native agarose gels. Proteolytic removal of the core histone tail domains inhibits but does not completely abolish rSir3p binding to nucleosomal arrays. The linker DNA in the supershifted complexes remains freely accessible to restriction endonuclease digestion, suggesting that both the tail domains and nucleosomal DNA contribute to rSir3p–chromatin interactions. Together these data indicate that rSir3p cross-links individual nucleosomal arrays into supramolecular assemblies whose physical properties transcend those of typical 10-nm and 30-nm fibers. Based on these data we hypothesize that Sir3p functions, at least in part, by mediating reorganization of the canonical chromatin fiber into functionally specialized higher order chromosomal domains.

Influencia de la intensidad de clara y tipo de dosel en el ciclo interno de nutrientes y el crecimiento secundario de pino silvestre en un bosque mixto de los Pirineos occidentales

Resumen de Tesis Doctoral, Universidad Pública de Navarra, Noviembre de 2012, Dirección: Drs. J. Bosco Imbert y Federico J. Castillo.

Marie Antoinette and the end of the old régime;

Covers period 1781-October 1789. Continued by "Marie Antoinette at the Tuilleries" and "Marie Antoinette and the downfall of royalty".

The youth of the Duchess of Angoulême,

Mode of access: Internet.

Sands, palms and minarets,

Map on lining-paper.

A classification of the larvae and puparia of the Syrphidae of Illinois, exclusive of aquatic forms.

Issued also as thesis (PH.D.) University of Illinois.

Madame de Maintenon,

Mode of access: Internet.

Les Cochin,

Initials; head and tail pieces.

Dissertations et mémoires sur différens sujets d'antiquité et d'histoire : mis en ordre et publiés d'après les manuscrits de feu M. Pasumot, ingénieur-géographe du roi /

A collection of newly published articles, supplemented by offprints from various publications.

Thesis diaetetica quam pro baccalaureatu propugnabat ... an sanitati faciat decumbendi modus? /

Mode of access: Internet.

Traité de l'hydrocèle : cure radicale de cette maladie et traitement de plusieurs autres qui attaquent les parties de la génération de l'homme /

Mode of access: Internet.

Oeuvres choisies /

Contiene: Le père de famille / Diderot .- L'honnête criminel ; Les deux avares / Fenouillot de Falbaire .- Le jaloux sans amour / Imbert.

Les journées amusantes dediées au roi /

Mode of access: Internet.

Le conservateur du sang humain, ou la saignee demontree toujours pernicieuse & souvent mortelle /

Mode of access: Internet.