Desidratação e retificação de etanol produzido a partir da amêndoa do caroço da manga

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Estudo dos mecanismos envolvidos no processo de diferenciação em linhagem osteogênica de células-tronco mesenquimais da medula óssea de ratos Wistar e ratos espontaneamente hipertensos (SHR)

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Estudo das células primárias dentais, viabilidade celular e bioatividade de materiais á base de silicato de cálcio

Pós-graduação em Odontologia - FOAR

Caracterização química, física e anatômica do bambu Guadua tagoara para polpação Kraft

Pós-graduação em Engenharia Mecânica - FEG

Análise química, citotoxicidade e biocompatibilidade de cimentos de ionômero de vidro

Objetivo: O presente trabalho, dividido em três estudos, teve como objetivo geral identificar e quantificar a liberação de componentes e avaliar a citotoxicidade e a biocompatibilidade de cimentos de ionômero de vidro (CIVs). Método: Para o estudo 1, extratos dos CIVs Vitrebond (VB), Fuji Lining LC (FL), Vitremer (VM), Fuji II LC (FII), Ketac Fil Plus (KF) e Ketac Molar Easymix (KM) foram obtidos pela imersão de corpos-de-prova em meio de cultura celular (DMEM). Esses extratos (n=9 por grupo) foram analisados por eletrodo específico quanto à presença de flúor e por espectrometria de absorção atômica quanto à presença de alumínio e zinco. HEMA e iodobenzeno foram identificados por CG/EM (n=6). Para o estudo 2, células MDPC-23 foram colocadas em contato com os extratos dos CIVs por 24 horas. Em seguida, foram avaliadas a atividade da desidrogenase succínica (SDH) (n=8), a produção de proteína total (PT) (n=8), a atividade da fosfatase alcalina (FAL) (n=8) e a morfologia celular (n=2). Para o estudo 3, tubos de polietileno (n=24 por grupo) foram preenchidos com os CIVs e implantados no tecido subcutâneo de 42 ratos. Como grupo controle foi utilizada a guta-percha. Após 7 ou 15 dias de pós-operatório, metade dos espécimes de cada grupo e período (n=6) foi preparada para análise histológica, e os demais (n=6) para análise da expressão de genes que codificam para IL-1? e TNF-?. Resultados: Os extratos de todos os CIVs apresentaram uma concentração de flúor significativamente maior do que o meio de cultura DMEM (controle), tendo o VB liberado maior quantidade, estatisticamente significante, do que os demais CIVs. O VB foi, também, o único material que liberou quantidades relativamente altas de alumínio e de zinco. O HEMA foi identificado nos extratos de todos os CIVs modificados por resina (VB, FL, VM e FII), e o iodobenzeno... (Resumo completo, clicar acesso eletrônico abaixo)

Síntese e atividade biológica de complexos de metais de transição com hidrolisados proteicos da clara do ovo

Trabalho trata da síntese e caracterização de quelatos inéditos de prata, paládio e platina contendo proteínas hidrolisadas da clara de ovo. A ideia consiste em preparar e utilizar quelatos metálicos contendo ligantes de origem natural, como metalofármacos. A pesquisa realizada pode ser dividida em três etapas. A primeira, referente à hidrólise e análise do hidrolisado proteico da clara de ovo. Diversos métodos e condições experimentais foram testadas a fim de se encontrar as condições de hidrólise mais adequadas (natureza da enzima, tempo, pH, temperatura, relação enzima:substrato) para a obtenção de aminoácidos livres e/ou pequenos peptídeos. A segunda parte consistiu na obtenção de compostos de coordenação de platina, prata e paládio contendo o hidrolisado proteico. Nessa etapa, diferentes rotas sintéticas e condições experimentais também foram utilizadas. Além disso, a espectroscopia vibracional no infravermelho foi utilizada nessa etapa para investigar as características estruturais (particularmente, os grupos funcionais presentes) dos compostos metálicos obtidos bem como da matéria prima utilizada (clara de ovo). Após inúmeras tentativas de síntese, três amostras promissoras, do ponto de vista da Química de Coordenação, foram obtidas, a saber, CO-Pd2, CO-Pd9 e CO-Ag3. Ensaios biológicos preliminares, que consistiu na terceira e última parte deste trabalho, foram realizados no sentido de avaliar a capacidade inibitória de crescimento das bactérias S. aureus e E. coli, através da determinação da Concentração Inibitória Mínima (CIM) dos compostos metálicos obtidos.

Estudo da qualidade da água distribuída pelo poço artesiano da FEG em relação à água servida pelo abastecimento público de Guaratinguetá

We conducted a study of the quality of water distributed by Artesian Well in relation to water supply served by the public for purposes of human consumption of UNESP Campus Guaratinguetá. Two sections of the network were randomly chosen and monitored for 105 days: the stretch of the pipeline started off the Water Treatment Station of the Autonomous Service of Water, Sewage and waste in the Municipality of Guaratinguetá and ended in a point consumption of the Child Family Center, while the section B began at the output of an artesian well and finished in a point of consumption of the Department of Civil Engineering. It is the deterioration of water quality in parts A and B, through comparative analysis of Bicarbonates, Total Hardness, Total Iron, free residual chlorine, pH, Total Residual, Turbidity, Conductivity, Color, Fecal Coliforms and Total Coliforms. Among the observations that can be highlighted: the Total Hardness was found in highest concentration in the extract; for Total Iron significant variations were obtained for both parts, and reduction of this parameter in two points of consumption analyzed, not the expected increase concentration, because the contact of water along the pipe, the CRL showed significant depletion to section A, which can be related to impurities in the water, the presence of biofilm on the walls of pipes, or because this is the stretch of greater extent to be covered by water for pH, this indicated that the water is alkaline and slightly acidic stretch in section B; Turbidity was found for the higher concentration to the passage, and a considerable reduction of this parameter at point consumption due to deposition of particulate matter along the stretch, the conductivity also showed a higher concentration in the extract, as well as an increase in this parameter at the point of consumption. Upon leaving ... (Complete abstract click electronic access below)

Estudo de rochas alcalinas intrusivas na região de Três Fontes - MG

Geological researches conducted in the past few years detected, through deep drill hole data, the presence of alkaline rocks in the region of Três Fontes-MG, where the Barbacena Group rocks, the Morro do Ferro Greenstone Belt rocks and Araxá/Canastra groups‟ rocks are exposed. This paper aimed the petrographic and chemical characterization of these alkaline rock types, which have not yet been described in the literature. Based on petrographic descriptions and geochemical and Scanning Electron Microscopy analysis, it was possible to characterize the rock in question as lamprophyre, rich in carbonates, phlogopite, pyroxene, olivine, titaniferous opaque minerals and apatite concentrations that reach 7%. This occurrence corresponds to an alkaline intrusion, which caused brecciation of host rocks, possibly indicating that the material is explosive, however, in the study area there was no evidence of volcanic activity on the surface

Atividade antiinflamatória intestinal da escopoletina e fraxetina nas fases aguda e crônica do processo inflamatório induzido por TNBS em ratos

A Doença Inflamatória Intestinal (DII) engloba, fundamentalmente, duas doenças distintas: a Doença de Crohn (DC) e a Colite Ulcerativa (CU), ambas caracterizadas por uma inflamação crônica do intestino, com períodos de exacerbação seguidos de intervalos prolongados de remissão dos sintomas. Apesar da DII ser objeto de pesquisa há várias décadas, a sua etiologia ainda é desconhecida e seu tratamento envolve uma série de fármacos com sérios efeitos colaterais, especialmente quando usado cronicamente. As cumarinas representam uma importante classe de compostos fenólicos naturais que apresentam inúmeras propriedades farmacológicas importantes para que um produto seja potencialmente ativo no tratamento da DII. Dentre as inúmeras cumarinas conhecidas e estudadas, dados obtidos em nosso laboratório demonstram que algumas cumarinas apresentam efeitos protetores na fase aguda e preventiva do processo inflamatório intestinal induzido por TNBS (ácido trinitrobenzenosulfônico) em ratos, reduzindo a incidência de diarréia, aderência e obstrução intestinal, além de manterem os níveis de glutationa e reduzirem a atividade da fosfatase alcalina e da mieloperoxidase colônica. Os objetivos do presente projeto são determinar a atividade antiinflamatória intestinal da escopoletina e fraxetina na fase aguda e crônica com recidiva de colite ulcerativa induzida por TNBS tendo-se em vista a determinação de sua atividade curativa. Para tanto, serão avaliados os parâmetros clínicos gerais (consumo de alimento e peso corporal), parâmetros macroscópicos da lesão (determinação de índice de lesão, relação peso-comprimento colônico) e bioquímicos (avaliação das atividades da mieloperoxidase, fosfatase alcalina, níveis de proteínas e glutationa total)

Produção de hidrolisado enzimático de farinha de batata-doce para fermentação etanólica

Recently there is a great quest of producing alcohol from starchy resources, replacing the sugar cane. The most common starchy sources are cassava, maize and sweet potatoes and a lot of research are been realized with excellent results. In this work it was evaluated the influence of the concentration of dry matter on the enzymatic hydrolysis process of starch from sweet potato for ethanol production. Through the sweet potato was produced a flour using a low-cost method and easy operation equipments. The sweet potato flour was characterized physical and chemically and from these results was prepared the treatments for enzymatic hydrolysis. The experimental design considered as independent variable the dry matter concentration of the sweet potato flour in 3 levels; 10, 15 and 20% in the formulation of suspensions. The other variables were keeping constant being: temperature in the 1° hydrolysis step of 90°C and time of 2 hours; temperature in the 2° saccharification step of 60°C and time of 17 hours. The hydrolysates obtained at the three assays were transferred to six liter enlerynmeyer and inoculated with a biologic catalyst, Saccharomyces, dehydrated yeasts of Saccharomyces cerevisiae CAT 1, at a rate of 5% in weight. The flasks were placed in a shaker type orbital with controlled temperature of 30°C during a time of 15 hours. The initial reducer sugars concentration and respective ethanol concentrations in wine were: 11.2% glucose and 2.16% ethanol in the suspension with 10% of dry matter; 13.5% glucose and 4.39% ethanol with 15% and 17.5% glucose and 6.03% ethanol in suspension with 20% of dry matter. ix The results showed that the higher percentage of dry matter carried out to higher sugar yield in hydrolyzed. It was possible observed that products quality improved with a higher concentration of dry matter

Desenvolvimento e caracterização de compósitos de fibras naturais modificadas e híbridos: fibras da coroa do abacaxi/polipropileno e fibras da coroa do abacaxi/fibras de vidro/polipropileno

The concern with the environment preservation has done with that researchers as well as industries invest in the search for materials that come from renewable sources. Natural fibers, because they are ecologically correct and have low cost, have been studied as a possible substitute, even if partial, of synthetic fibers in the development of polymeric composites. In this context, the hybrid composites (natural/synthetic) increase considerably the range of application of natural composites. The auto industry, in its constant quest for good mechanical properties materials which are developed with sustainability, has in composites with hybrid reinforcement a very viable alternative. In the present work, the nature Crown pineapple fibers and nature Crown pineapple fibers treated with alkaline solution were studied in order to evaluate the influence of chemical treatment in its properties. For this techniques were used x-ray diffractometry, Thermogravimetry and Infrared Spectroscopy (FTIR). Composites have been developed using polypropylene, reinforced with pineapple fibers and pineapple fibers hybrids/glass fibres, both with levels of 5 and 10%. These composites were analyzed by Thermogravimetry techniques and tested by traction. The realization of this work indicated that although the chemical treatment did not affect the thermal stability of the fibers, caused an increase in crystallinity index fibers and decreased its hydrophilic. The tests performed on composite indicated that the composites process was suitable because it provided good dispersion of the polymer matrix. The addition of natural fibers from the pineapple's Crown, in a proportion of 10%, provided the greatest increase in modulus of elasticity (27%) when compared to the pure polymer

Exames laboratoriais importantes no diagnóstico da Piometra canina

Canine Pyometra is a uterine disease that occurs in sexually mature bitches, with higher incidence in nulliparous and animals over 4 years and is characterized by presenting an accumulation of pus in the uterine lumen, usually occurring in diestrus. Laboratory tests are important tools for the detection of metabolic abnormalities associated with sepsis and renal function, which are serious consequences of pyometra. In blood the main findings are normochromic non-regenerative anemia, presence of dehydration, and sometimes thrombocytopenia. The WBC count may be normal but most often occurs a neutrophilic leukocytosis with a left shift, monocytosis and the presence of toxic neutrophils. In less than 1 / 3 of the animals the presence of azotemia is present and a density lower than 1035 is detected in the urine of almost 90% of bitches which may be in normal range at the onset of the desease. Urinary protein loss is rare but the protein may be elevated in the reagent strip due to urinary contamination by uterine secretion. The increase of gamma-glutamyltransferase (GGT), alkaline phosphatase (ALP), aspartate aminotransferase (AST) and creatine kinase (CK) may be present, indicating disorders in the liver. Currently, additional laboratory tests are being studied for the diagnosis of pyometra and its prognosis, such as the measurement of C-reactive protein and fibrinogen for monitoring the recovery of the inflammatory process and the urine electrophoresis to characterize the origin of proteinuria in these animals . The aim of this work is to review the literature on the main laboratory tests that aid the diagnosis of Pyometra

Estudo da reação de oxidação de metanol sobre fases intermetálicas ordenadas de AuIn, AuSn e AuSb2 em meio alcalino

The present work assessment materials can be used as fuel cells electrocatalysts. The alkaline fuel cell though was less studied, has some advantages compared to the acid configuration. The materials assesment were Au polycrystalline and intermetallics ordered phases of AuIn, AuSn and AuSb2. Your electrocatalytic properties were studied across cyclic voltametry and chronoamperometry techniques in Sodium Hydroxide 0,15M and Metanol 0,15M solution. The results obtained show a more efficiency to intermetallic AuIn as electrocatalyst for the oxidation reaction of methanol in alkaline medium, it showed high levels of current density and on set potential less positive compared to Au polycrystalline. The intermetallic AuSn showed activity just higher concentrations of methanol. Except AuSb2, who represented himself unstable in alkaline media, the intermetallics AuIn and AuSn present a promising future as anode materials for the oxidation in alkaline medium

Cinética de hidrólise de GPTS sob estimulação ultrassônica

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Otimização de um método cromatográfico de análise de pesticidas organoclorados em amostras de peixes

Considering that persistent organic pollutants (POPs) including synthetic substances belonging to differents organic functions, including organochlorine pesticides (OCPs), which are the most persistent and bioaccumulative, with high toxicity to humans and animals. Accordingly environmental and biological monitoring is necessary, in order to have greater control regarding the irresponsible use of these products. Though there are several analytical methodology reported in the literature to make determinations of this pesticide, they present some difficulties, requiring several steps to make the clean up of the sample. The proposed project aims to optimize a new analytical method that allows to perform the extraction of organochlorine pesticides in fish tissues, employing acetone as solvent assisted by ultrasound bath, making the method more quickly and not requiringfurther steps to purify the sample. Were analyzed the recoveries of pesticides in study in samples of tilapia average values: Heptachlor=84,7±9,8%; Aldrin=87,3±3,5%; Endosulfan=101,4±18,6%; DDE= 90,5± 3,3%, Endrin=102,2±13,8%., DDD=92,1±19,9%; DDT=90,8±6,8%. The real samples showed values granted for our legislation and low values of error