Comparative study of the whisky aroma profile based on headspace solid phase microextraction using different fibre coatings

A dynamic headspace solid-phase microextraction (HS-SPME) and gas chromatography coupled to ion trap mass spectrometry (GC–ITMS) method was developed and applied for the qualitative determination of the volatile compounds present in commercial whisky samples which alcoholic content was previously adjusted to 13% (v/v). Headspace SPME experimental conditions, such as fibre coating, extraction temperature and extraction time, were optimized in order to improve the extraction process. Five different SPME fibres were used in this study, namely, poly(dimethylsiloxane)(PDMS),poly(acrylate)(PA),Carboxen-poly(dimethylsiloxane)(CAR/PDMS),Carbowax-divinylbenzene(CW/DVB)and Carboxen-poly(dimethylsiloxane)-divinylbenzene (CAR/PDMS/DVB). The best results were obtained using a 75 m CAR/PDMS fibre during headspace extraction at 40◦C with stirring at 750rpm for 60min, after saturating the samples with salt. The optimised methodology was then appliedtoinvestigatethevolatilecompositionprofileofthreeScotchwhiskysamples—BlackLabel,BallantinesandHighlandClan.Approximately seventy volatile compounds were identified in the these samples, pertaining at several chemical groups, mainly fatty acids ethyl esters, higher alcohols, fatty acids, carbonyl compounds, monoterpenols, C13 norisoprenoids and some volatile phenols. The ethyl esters form an essential group of aroma components in whisky, to which they confer a pleasant aroma, with “fruity” odours. Qualitatively, the isoamyl acetate, with “banana” aroma,wasthemostinteresting.Quantitatively,significantcomponentsareethylestersofcaprilic,capricandlauricacids.Thehighestconcentration of fatty acids, were observed for caprilic and capric acids. From the higher alcohols the fusel oils (3-methylbutan-1-ol and 2.phenyletanol) are the most important ones.

Characterization of Post-Consumer PET after Removal of the Original Surface: Influence of Raw Material

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Avaliação da atividade antimicrobiana de sistemas emulsionados contendo óleos naturais para o tratamento de infecções cutâneas

Natural oils have shown a scientific importance due to its pharmacological activity and renewable character. The copaiba (Copaifera langsdorffii) and Bullfrog (Rana catesbeiana Shaw) oils are used in folk medicine particularly because the anti-inflammatory and antimicrobial activities. Emulsion could be eligible systems to improve the palatability and fragrance, enhance the pharmacological activities and reduce the toxicological effects of these oils. The aim of this work was to investigate the antimicrobial activity of emulsions based on copaiba (resin-oil and essential-oil) and bullfrog oils against fungi and bacteria which cause skin diseases. Firstly, the essential oil was extracted from copaiba oil-resin and the oils were characterized by gas chromatography coupled to a mass spectrometry (GC-MS). Secondly, emulsion systems were produced. A microbiological screening test with all products was performed followed (the minimum inhibitory concentration, the bioautography method and the antibiofilm determination). Staphylococcus aureus, S. epidermidis, Pseudomonas aeruginosa, Candida albicans, C. parapsilosis, C. glabrata, C. krusei and C. tropicalis American Type Culture Collection (ATCC) and clinical samples were used. The emulsions based on copaiba oil-resin and essential oil improved the antimicrobial activity of the pure oils, especially against Staphylococcus e Candida resistant to azoles. The bullfrog oil emulsion and the pure bullfrog oil showed a lower effect on the microorganisms when compared to the copaiba samples. All the emulsions showed a significant antibiofilm activity by inhibiting the cell adhesion. Thus, it may be concluded that emulsions based on copaiba and bullfrog oils are promising candidates to treatment of fungal and bacterial skin infections

Root system of Panicum maximum Jacq. cv. IPR-86 Mil(e)over-capnio fertilized with nitrogen and submitted to rotational stocking

The effect of nitrogen on the root system of the species Panicum maximum Jacq. cv. IPR-86 Mil (e) over cap nio, under grazing, was evaluated. The N rates were 0; 150; 300 and 450 kg/ha. year. The root density was evaluated during pregrazing at five years of successive N application, in three depths (0-10; 10-20 and 20-40 cm) and the root growth at 7, 14, 21, and 35 days after grazing. The grazing method adopted was rotational stocking. Root length and root mass densities in pre-and post-grazing presented maximum values at rates 204, 206, 192, and 197 kg/ha of N, respectively. The root growth (in root length density) increased, on average, until 29 days after grazing at rates 0, 150, and 300 kg/ha; at 450 kg/ha N rate, the increase was linear. Independently of N rates, around 60 and 25% of IPR-86 Mil (e) over cap nio cultivar root system was concentrated in 0-10 and 10-20 cm depth, respectively.

Produção de fitomassa, teor e produtividade do óleo essencial de Baccharis dracunculifolia DC. em função da adubação orgânica

Objetivou-se avaliar a produção de fitomassa, teor e produtividade do óleo essencial de Baccharis dracuculifolia DC., em função de doses de adubo orgânico, e foram utilizadas mudas produzidas por semente, e mantidas sob sombrite pelo período de 84 dias até serem transplantadas para o campo, no espaçamento de 1,0 x 1,0 m, com irrigação por gotejamento. O delineamento estatístico utilizado foi em blocos ao acaso, com quatro repetições, e as doses de composto orgânico foram: 0, 10, 20, 30, 40 e 50 t ha-1. Foi realizada uma colheita, aos 150 dias após o transplante das mudas. O óleo essencial foi extraído por hidrodestilação e analisado em cromatógrafo a gás acoplado a espectrômetro de massas (Shimadzu, QP-5000). Todos os resultados foram submetidos à análise de variância pelo teste F seguido da análise de regressão e o teste Tukey para os dados: massa seca útil da parte aérea, teor e produtividade do óleo essencial. Para as variáveis massa seca total e massa seca útil da parte aérea, as dosagens estudadas influenciaram as plantas, que apresentaram os melhores resultados na dose 50 t ha-1. Para teor de óleo não houve influência significativa para as dosagens estudadas, porém a dose 30 t ha-1 apresentou o melhor resultado. Entretanto para a produtividade do óleo essencial, as dosagens estudadas influenciaram as plantas, que na dosagem 30 t ha-1 obtiveram os melhores resultados.

Thin films generated by plasma immersion ion implantation and deposition of hexamethyldisilazane mixed with nitrogen in different proportions

In this work, it was used a plasma system composed of a cylindrical stainless steel reactor, a radio-frequency (13.56MHz) power source fixed at either 25 W or 70 W, a power source with a negative bias of 10kV and a 100Hz pulse. The system worked at an operational pressure of 80mTorr which consisted of varying concentrations of the monomer HMDSN and gaseous nitrogen in ratios: HMDSN (mTorr)/nitrogen (mTorr) from 70/10 to 20/60 in terms of operational pressure. The structural characterization of the films was done by FTIR spectroscopy. Absorptions were observed between 3500 cm(-1) to 3200 cm(-1), 3000 cm(-1) to 2900 cm(-1), 2500 cm(-1) to 2000 cm(-1), 1500 cm(-1) to 700 cm(-1), corresponding, respectively, to OH radicals, C-H stretching bonds in CH2 and CH3 molecules, C-N bonds, and finally, strain C-H bonds, Si-CH3 and Si-N groups, for both the 70 W and the 25 W. The contact angle for water was approximately 100 degrees and the surface energy is near 25mJ/m(2) which represents a hydrophobic surface, measured by goniometric method. The aging of the film was also analyzed by measuring the contact angle over a period of time. The stabilization was observed after 4 weeks. The refractive index of these materials presents values from 1.73 to 1.65 measured by ultraviolet-visible technique.

Surface Corrosion of Ti-16Si-4B Powder Alloy Implanted With Nitrogen by Plasma-Based Technique

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Tratamento eletroquímico de água produzida sintética para remoção de hidrocarbonetos policíclicos aromáticos

The aim of this work is the treatment of produced water from oil by using electrochemical technology. Produced water is a major waste generated during the process of exploration and production in the oil industry. Several approaches are being studied aiming at the treatment of this effluent; among them can be cited the biological process and chemical treatments such as advanced oxidation process and electrochemical treatments (electrooxidation, electroflotation, electrocoagulation, electrocoagulation). This work studies the application of electrochemical technology in the treatment of the synthetic produced water effluent through the action of the electron, in order to remove or transform the toxic and harmful substances from the environment by redox reactions in less toxic substances. For this reason, we used a synthetic wastewater, containing a mixture H2SO4 0,5M and 16 HPAs, which are: naphthalene, acenaphthylene, acenaphthene, fluorene, phenanthrene, anthracene, fluoranthene, pyrene, benzo (a) anthracene, chrysene, benzo(b)fluoranthene, benzo(k) fluoranthene, benzo(a)pyrene, indeno(1,2,3-cd)pyrene, dibenzo(a, h)anthracene, benzo(g, h, i)perylene. Bulk electrochemical oxidation experiments were performed using a batch electrochemical reactor containing a pair of parallel electrodes, coupled with a power supply using a magnetic stirrer for favoring the transfer mass control. As anodic material was used, a Dimensionally Stable Anode (DSA) of Ti/Pt, while as cathode was used a Ti electrode. Several samples were collected at specific times and after that, the analysis of these samples were carried out by using Gas Chromatography Coupled to Mass Spectrometry (GC - MS) in order to determine the percentage of removal. The results showed that it was possible to achieve the removal of HPAs about 80% (in some cases, more than 80%). In addition, as an indicator of the economic feasibility of electrochemical treatment the energy consumption was analyzed for each hour of electrolysis, and based on the value kWh charged by ANEEL, the costs were estimated. Thus, the treatment costs of this research were quite attractive

Estudo dos principais fatores que influenciam na solubilização dos hpas em águas

Polycyclic aromatic hydrocarbons (PAHs) constitute a family of compounds characterized by having two or more condensed aromatic rings and for being a class of substances that are widely distributed in the environment as a complex mixture, being very persistent in the environment due to its low solubility in water. The application of chemometric methods to analytical chemistry has provided excellent results in studying the solubility of PAHs in aqueous media in order to understand the mechanisms involved in environmental contamination. The method consists in analyzing the solubilization of PAHs from diesel oil in water varying parameters such as stirring time, volume of oil added and pH, using a full factorial design of two levels and three factors. PAHs were extracted with n-hexane and analyzed by fluorescence spectroscopy because they have molecular characteristics fluorescent due to the large number of condensed rings and links, and gas chromatography coupled to a mass spectrometer (GC-MS). The results of fluorescence analysis showed that only the stirring time and pH influenced the solubility of PAHs in diesel fuel. How is a non-selective technique for the study of fluorescence was performed on form and semi-quantitative. And for the chromatographic analysis the results showed that the solubility of the different PAHs is influenced differently so that you can classify them into groups by the results of the effects

Yield, chemical composition and chlorophyll relative content of Tanzania and Mombaça grasses irrigated and fertilized with nitrogen after corn intercropping

Avaliou-se o efeito da ausência e da aplicação de três doses de nitrogênio (50, 100 e 200 kg ha-1 de N) e quatro épocas de corte no inverno/primavera (julho a outubro) sobre a produtividade de massa seca (PMS), os teores relativos de clorofila (ICF _ índice de clorofila foliar) e os teores de nutrientes digestíveis totais (NDT), proteína bruta (PB), fibra em detergente neutro (FDN), fibra em detergente ácido (FDA) e lignina, bem como suas respectivas correlações nos capins tanzânia e mombaça após o consórcio com milho em um Latossolo Vermelho distroférrico. O delineamento experimental utilizado foi o de blocos casualizados, em parcelas subdivididas, com quatro repetições. As maiores PMS ocorreram com o aumento do fotoperíodo (a partir de agosto), no entanto, as respostas à adubação nitrogenada ao longo dos cortes diferiram entre e dentre os capins. em sistema de integração lavoura-pecuária irrigado sob condições de cerrado, é tecnicamente viável o estabelecimento dos capins tanzânia e mombaça em consórcio com o milho no momento da semeadura ou por ocasião da adubação nitrogenada de cobertura, visto que, mesmo na ausência de adubação nitrogenada, foi produzida quantidade satisfatória de forragem, com PMS média de 2.000 kg ha-1 por corte na época de maior escassez de volumoso para os animais (inverno/primavera). A adubação nitrogenada após a colheita do milho eleva a PMS e melhora a composição bromatológica dos capins, com aumento dos teores relativos de clorofila e PB no inverno/primavera, além de aumento dos teores de NDT e redução dos teores de FDN e FDA até o mês de setembro. O índice de clorofila foliar pode ser utilizado para estimar a PMS e o teor de PB, bem como indicar a necessidade de adubação nitrogenada dos capins tanzânia e mombaça submetidos a corte.

Search for the rare decay B-s(0)->phi mu(+)mu(-) with the D0 detector

We present a search for the flavor-changing neutral current decay B-s(0)->phi mu(+)mu(-) using about 0.45 fb(-1) of data collected in p (p) over bar collisions at root s=1.96 TeV with the D0 detector at the Fermilab Tevatron Collider. We find an upper limit on the branching ratio of this decay normalized to B-s(0)-> J/psi phi of B(B-s(0)->phi mu(+)mu(-))/B(B-s(0)-> J/psi phi)< 4.4x10(-3) at the 95% C.L. Using the central value of the world average branching fraction of B-s(0)-> J/psi phi, the limit corresponds to B(B-s(0)->phi mu(+)mu(-))< 4.1x10(-6) at the 95% C.L., the most stringent upper bound to date.

Search for single production of scalar leptoquarks in p(p)over-bar collisions decaying into muons and quarks with the D0 detector

lWe report on a search for second generation leptoquarks (LQ(2)) which decay into a muon plus quark in (p) over barp collisions at a center-of-mass energy of root s = 1.96 TeV in the DO detector using an integrated luminosity of about 300 pb(-1). No evidence for a leptoquark signal is observed and an upper bound on the product of the cross section for single leptoquark production times branching fraction into a quark and a muon was determined for second generation scalar leptoquaiks as a function of the leptoquark mass. This result has been combined with a previously published DO search for leptoquark pair production to obtain leptoquark mass limits as a function of the leptoquark-muon-quark coupling, lambda. Assuming lambda = 1, lower limits on the mass of a second generation scalar leptoquark coupling to a u quark and a muon are m(LQ2) > 274 GeV and m(LQ2) > 226 GeV for beta = 1 and beta = 1/2, respectively. (c) 2007 Elsevier B.V. All rights reserved.

Multivariate searches for single top quark production with the D0 detector

We present a search for electroweak production of single top quarks in the s-channel (p (p) over bar -> t (b) over bar +X) and t-channel (p (p) over bar -> tq (b) over bar +X) modes. We have analyzed 230 pb(-1) of data collected with the D0 detector at the Fermilab Tevatron Collider at a center-of-mass energy of root s=1.96 TeV. No evidence for a single top quark signal is found. We set 95% confidence level upper limits on the production cross sections, based on binned likelihoods formed from a neural network output. The observed (expected) limits are 6.4 pb (4.5 pb) in the s-channel and 5.0 pb (5.8 pb) in the t-channel.

Measurement of the Lambda(0)(b) lifetime in the decay Lambda(0)(b)-> J/psi Lambda(0) with the D0 detector

We present measurements of the Lambda(b)(0) lifetime in the exclusive decay channel Lambda(b)(0)-> J/psi Lambda(0), with J/psi ->mu(+)mu(-) and Lambda(0)-> p pi(-), the B-0 lifetime in the decay B-0-> J/psi K-S(0) with J/psi ->mu(+)mu(-) and K-S(0)->pi(+)pi(-), and the ratio of these lifetimes. The analysis is based on approximately 250 pb(-1) of data recorded with the D0 detector in p (p) over bar collisions at root s = 1.96 TeV. The Lambda(b)(0) lifetime is determined to be tau(Lambda(b)(0))=1.22(-0.18)(+0.22)(stat)+/- 0.04(syst) ps, the B-0 lifetime tau(B-0)=1.40(-) (+0.11)(0.10)(stat)+/- 0.03(syst) ps, and the ratio tau(Lambda(b)(0))/tau(B-0)=0.87(-) (+0.17)(0.14)(stat)+/- 0.03(syst). In contrast with previous measurements using semileptonic decays, this is the first determination of the Lambda(b)(0) lifetime based on a fully reconstructed decay channel.

Search for large extra spatial dimensions in dimuon production with the D0 detector

We present the results of a search for the effects of large extra spatial dimensions in p (p) over bar collisions at root s = 1: 96 TeV in events containing a pair of energetic muons. The data correspond to 246 pb(-1) of integrated luminosity collected by the D0 experiment at the Fermilab Tevatron Collider. Good agreement with the expected background was found, yielding no evidence for large extra dimensions. We set 95% C. L. lower limits on the fundamental Planck scale between 0.85 and 1.27 TeV within several formalisms. These are the most stringent limits achieved in the dimuon channel to date.