Seawater carbonate chemistry and calcification during tropical reef studies at Moorea (French Polynesia), 1997

Community metabolism and air-sea carbon dioxide (CO2) fluxes were investigated in July 1992 on a fringing reef at Moorea (French Polynesia). The benthic community was dominated by macroalgae (85% substratum cover) and comprised of Phaeophyceae Padina tenuis (Bory), Turbinaria ornata (Turner) J. Agardh, and Hydroclathrus clathratus Bory (Howe); Chlorophyta Halimeda incrassata f. ovata J. Agardh (Howe); and Ventricaria ventricosa J. Agardh (Olsen et West), as well as several Rhodophyta (Actinotrichia fragilis Forskál (Børgesen) and several species of encrusting coralline algae). Algal biomass was 171 g dry weight/m**2. Community gross production (Pg), respiration (R), and net calcification (G) were measured in an open-top enclosure. Pg and R were respectively 248 and 240 mmol Co2/m**2/d, and there was a slight net dissolution of CaCO3 (0.8 mmol/m**2/d). This site was a sink for atmospheric CO2 (10 ± 4 mmol CO2/m**2/d), and the analysis of data from the literature suggests that this is a general feature of algal-dominated reefs. Measurement of air-sea CO2 fluxes in open water close to the enclosure demonstrated that changes in small-scale hydrodynamics can lead to misleading conclusions. Net CO2 evasion to the atmosphere was measured on the fringing reef due to changes in the current pattern that drove water from the barrier reef (a C02 source) to the study site.

Seawater carbonate chemistry and calcification during a study of barrier reef flat in Moorea, French Polynesia, 1998

The relative contribution of soft bottoms to the community metabolism (primary production, respiration and net calcification) of a barrier reef flat has been investigated at Moorea (French Polynesia). Community metabolism of the sedimentary area was estimated using in situ incubations in perspex chambers, and compared with estimates of community metabolism of the whole reef flat obtained using a Lagrangian technique (Gattuso et al., 1996. Carbon flux in coral reefs. 1. Lagrangian measurement of community metabolism and resulting air-sea CO2 disequilibrium. Mar. Ecol. Prog. Ser. 145, 109-121). Net organic carbon production (E), respiration (R) and net calcification (G) of sediments were measured by seven incubations performed in triplicate at different irradiance. Respiration and environmental parameters were also measured at four randomly selected additional stations. A model of Photosynthesis-irradiance allowed to calculate oxygen (O2), organic carbon (CO2) and calcium carbonate (CaCO3) evolution from surface irradiance during a diel cycle. As chlorophyll a content of the sediment was not significantly different between stations, primary production of the sediment was considered as homogeneous for the whole lagoon. Thus, carbon production at the test station can be modelled from surface light irradiance. The modelled respiration was two times higher at the test station than the mean respiration of the barrier reef, and thus underestimated sediment contribution to excess production. Sediments cover 40-60% of the surface and accounted for 2.8-4.1% of organic carbon excess production estimated with the modelled R and 21-32% when mean R value was considered. The sedimentary CaCO3 budget was a very minor component of the whole reef budget.

In Situ effects of low pH and elevated HCO3 on juvenile massive Porites spp. in Moorea, French Polynesia

Juvenile colonies of massive Porites spp. were exposed to manipulated pH and bicarbonate ([HCO3-]) in situ to test the hypothesis that ocean acidification (OA) does not affect respiration and calcification. Incubations lasted 28 h and exposed corals to ambient temperature and light with ecologically relevant water motion. Three treatments were applied: (1) ambient conditions of pH 8.04 and 1751 µmol HCO3- kg(-1) (Treatment 1), (2) pCO2-induced ocean acidification of pH 7.73 and 2011 µmol HCO3- kg(-1) (Treatment 2), and (3) pCO2 and HCO3--enriched seawater of pH 7.69 and 2730 µmol HCO3- kg(-1) (Treatment 3). The third treatment providing elevated [HCO3-] was used to test for stimulatory effects of dissolved inorganic carbon on calcification under low pH and low saturation of aragonite (Omega arag), but it does not reflect conditions expected to occur under CO2-driven OA. Calcification of juvenile massive Porites spp. was affected by treatments, with an 81% elevation in Treatment 3 versus Treatment 1, but no difference between Treatments 1 and 2; respiration and the metabolic expenditure concurrent with calcification remained unaffected. These findings indicate that juvenile massive Porites spp. are resistant to short exposures to OA in situ, and separately, that they can increase calcification at low pH and low Omega arag if [HCO3-] is elevated. Juvenile Porites spp. may therefore be limited by dissolved inorganic carbon under ambient pCO2 conditions

La iglesia de Santiago de los españoles en Roma y su entorno entre los siglos XV y XIX. Una historia a través del dibujo

Plaza Navona representa una de las visitas obligadas de Roma, pero solo algunos advertirán en ella la presencia española en la sala de exposiciones del Instituto Cervantes o en la inmediata Libreria Española. Todavía serán menos los que se percatarán de la huella española dejada en aquella iglesia de fachada anónima situada, en el extremo sur de la plaza: la antigua iglesia de Santiago de los Españoles. La presente tesis pretende, utilizando el dibujo como guía, herramienta y fin del proceso de análisis y estudio, reconstruir el proceso de conformación y construcción de la que fue iglesia española principal, cuya fundación hace patente el destacado papel jugado por la “nación” castellana en Roma durante la Edad Media; y en torno a la que se aglutinaron las actividades religiosas, diplomáticas y financieras de los castellanos que vivieron en la actual capital italiana. Se intentará recrear en el tiempo la que es hoy la iglesia de Nuestra Señora del Sagrado Corazón, sometiéndola a una restitución gráfica disciplinada, homogénea y objetiva en la medida de lo posible de las varias etapas que la han caracterizadas, desde su fundación hasta cuando en 1878 España se deshizo de ella, ya en ruina, vendiéndola. Como nos comenta Gaetano Moroni, de todas las comunidades nacionales que se encontraban en Roma la española parece ser efectivamente una de las más rica y prestigiosa. Aunque lo que no cuenta Moroni no haya sido todavía demostrado, dicho enunciado resulta de todas formas interesante puesto que pone el acento sobre el hecho de que ya desde el siglo X parece ser habitual de ocupar y reutilizar antiguas ruinas, usándolas como base para la construcción de hospitales para los peregrinos. Esta operación se hizo particularmente frecuente sobre todo antes del Gran Jubileo de 1450: de hecho desde la primera mitad del Quattrocento se fundan distintas iglesias y hospitales nacionales para acoger y prestar una adecuada asistencia y socorro a los innumerables peregrinos que llegaban a la ciudad, edificios que se van construyendo sobre los restos de antiguos edificios de época romana. Prueba de ello es en efecto la fundación originaria de la iglesia y hospital de los Españoles que, parte del conjunto de edificios que compone la Plaza Navona, situada en el corazón de Campo Marzio y cuya posición y forma corresponden a la del antiguo Estadio de Domiciano, y que ahora es en sus dimensiones, en su imagen arquitectónica y en su consistencia material, el resultado de la definición proyectual y de las transformaciones que se llevaron a cabo sobre lo que quedaba del antiguo templo español del siglo XV, entre finales del ‘800 y los años 30 del siglo XX . Transformaciones devastadoras, huellas grabadas o canceladas que encuentran una justificación en los acontecimientos históricos reflejados en el patrimonio urbano. El análisis de todas las fuentes permite trazar, si no la totalidad, buena parte de las modificaciones que la antigua iglesia de Santiago ha sufrido. La construcción del templo se puede dividir en tres momentos decisivos: una primera etapa de fundación en 1450-1478 en la que la iglesia tenía fachada y entrada en via de la Sapienza, hoy Corso Rinascimento; una segunda de significativa ampliación hacia Plaza Navona con una nueva fachada monumental hacia ese espacio público en 1496-1500; y una última importante ampliación entre 1525-1526, llevada a cabo por el arquitecto Antonio da Sangallo el Joven. Tras la intensa vida del templo, en el siglo XVIII, éste cae en ruina y finalmente es vendido en 1878 a la orden de los misioneros franceses de Nuestra Señora del Sagrado Corazón que la reconvierten en iglesia reformando totalmente el conjunto en 1881, según proyecto de Luca Carimini. En 1936, en plena fase de rectificación de trazados urbanos por obra del régimen fascista, según proyecto de Arnaldo Foschini, se mutila su extremidad hacia vía de la Sapienza dejando su estado tal y como se contempla en la actualidad. ABSTRACT The objective of this thesis is the reconstruction of the design and edification process -using drawings and sketches as a guide, tool and the end of the analytical process- of a church which was once the preeminent Spanish church in medieval Rome, known today as Nostra Signora del Sacro Cuore (Our Lady of the Sacred Heart). The founding of this church illustrates the important role held by the Castillian “nation” in Rome during the Middle Ages. It was the focal point of all the religious, diplomatic and economic activities of the Castillian community residing in today’s Italian capital. The aim of this proyect is a recreation the church in time by submitting it to a disciplined, homogenous and objective graphic restitution of the various stages most characteristic the temple, starting from its foundation until 1878 when, in a state of ruins, the church was finally sold off by Spain. Gaetano Moroni once commented that of all the international communities found in Rome, the Spanish community seemed to be one of the wealthiest and most prestigious. Such a statement proves interesting as it emphasizes that starting in the 10th century we see there was a widespread custom of occupying and reusing old ruins for use as the bases of new constructions of hospitals for pilgrims. This custom became especially frequent just before the Jubilee Year of 1450: in fact, in the first half of the Quattrocento we see the founding of many different national churches and hospitals which provided shelter and care to the countless pilgrims arriving in the city, buildings which were constructed on top of the ruins of ancient buildings left over from Roman times. Proof of this is the original foundation of the Spanish church and hospital forming part of the Piazza Navona, built upon and following the outline of the Stadium of Domitian, in the heart of Campo Marzio. Now, in its dimensions, its architectural image and its material substance, it represents the predominant result of the planning definitions and the transformations which affected the old 15th-century Spanish temple. Ocurring between the end of the 19th century and the 1930s, the transformations were devastating, erasing original peculiarities and engraving new ones, transformations made justifiable by the historical events reflected in its urban environs. Analyzing all sources allows us to trace, even if not in entirety, still a sizeable portion of the modifications undergone by the old Church of Saint James. The construction of the temple can be divided into three decisive moments: its foundation, from 1450 to 1478, when the church’s façade and main door looked out on to the Via della Sapienza, today’s central avenue of Corso del Rinascimento; the second stage being a major expansion towards the Piazza Navona (1496-1500) with a new, monumental façade facing the public space; and the third was the last significant expansion, carried out from 1525 to 1526 by the architect Antonio da Sangallo the Younger. Despite an intense and bustling life during the Modern Age, in the 18th century the church began to fall into ruin and was finally sold in 1878 to the order of French missionaries of Our Lady of the Sacred Heart, who reconverted it into a church and completely renovated the structure in 1881 in a project supervised by Luca Carimini. In 1936, the corrective urban redesign of Rome carried out by the fascist regime and implemented by Arnaldo Foschini mutilated the part bordering Via della Sapienza, leaving it as we see it today.

High-speed trains and tourists: what is the link? Evidence from the French and Spanish capitals

The objective of this paper is to analyse the factors influencing tourists? choice of a destination and the role of High Speed Rail (HSR) systems in this choice. The methodology proposed consists in analysing two capitals in Europe, i.e. Paris and Madrid where HSR services are important, to investigate the factors influencing holidaymakers in choosing these cities, and the role of HSR in this choice. The main outcome of this paper is to show that several factors influence the choice of a tourist, like the presence of architectural sites, the quality of promotion of the destination itself, the presence of events, and also HSR services. However we found that the HSR system has affected the choice of Paris and Madrid in a different way. Concerning the French case study, HSR is considered a real transport mode alternative among tourists, therefore HSR is chosen to reach Paris as well as for revisiting it. On the other hand, Madrid is chosen by tourists irrespective on the presence of HSR, while HSR is chosen for reaching cities close to Madrid. Data collected from the two surveys have been used for a further quantitative analysis. Models have been specified and calibrated to identify the factors influencing holidaymakers to revisit Paris and Madrid and the role of HSR in this choice has been highlighted.

Relationships of dehydroepiandrosterone sulfate in the elderly with functional, psychological, and mental status, and short-term mortality: A French community-based study

In human beings of both sexes, dehydroepiandrosterone sulfate (DHEAS) circulating in blood is mostly an adrenally secreted steroid whose serum concentration (in the micromolar range and 30–50% higher in men than in women) decreases with age, toward ≈20–10% of its value in young adults during the 8th and 9th decades. The mechanism of action of DHEA and DHEAS is poorly known and may include partial transformation into sex steroids, increase of bioavailable insulin-like growth factor I, and effects on neurotransmitter receptors. Whether there is a cause-to-effect relationship between the decreasing levels of DHEAS with age and physiological and pathological manifestations of aging is still undecided, but this is of obvious theoretical and practical interest in view of the easy restoration by DHEA administration. Here we report on 622 subjects over 65 years of age, studied for the 4 years since DHEAS baseline values had been obtained, in the frame of the PAQUID program, analyzing the functional, psychological, and mental status of a community-based population in the south-west of France. We confirm the continuing decrease of DHEAS serum concentration with age, more in men than in women, even if men retain higher levels. Significantly lower values of baseline DHEAS were recorded in women in cases of functional limitation (Instrumental Activities of Daily Living), confinement, dyspnea, depressive symptomatology, poor subjective perception of health and life satisfaction, and usage of various medications. In men, there was a trend for the same correlations, even though not statistically significant in most categories. No differences in DHEAS levels were found in cases of incident dementia in the following 4 years. In men (but not in women), lower DHEAS was significantly associated with increased short-term mortality at 2 and 4 years after baseline measurement. These results, statistically established by taking into account corrections for age, sex, and health indicators, suggest the need for further careful trials of the administration of replacement doses of DHEA in aging humans. Indeed, the first noted results of such “treatment” are consistent with correlations observed here between functional and psychological status and endogenous steroid serum concentrations.

Comparative Biochemistry of the Oxidative Burst Produced by Rose and French Bean Cells Reveals Two Distinct Mechanisms1

Cultured cells of rose (Rosa damascena) treated with an elicitor derived from Phytophthora spp. and suspension-cultured cells of French bean (Phaseolus vulgaris) treated with an elicitor derived from the cell walls of Colletotrichum lindemuthianum both produced H2O2. It has been hypothesized that in rose cells H2O2 is produced by a plasma membrane NAD(P)H oxidase (superoxide synthase), whereas in bean cells H2O2 is derived directly from cell wall peroxidases following extracellular alkalinization and the appearance of a reductant. In the rose/Phytophthora spp. system treated with N,N-diethyldithiocarbamate, superoxide was detected by a N,N′-dimethyl-9,9′-biacridium dinitrate-dependent chemiluminescence; in contrast, in the bean/C. lindemuthianum system, no superoxide was detected, with or without N,N-diethyldithiocarbamate. When rose cells were washed free of medium (containing cell wall peroxidase) and then treated with Phytophthora spp. elicitor, they accumulated a higher maximum concentration of H2O2 than when treated without the washing procedure. In contrast, a washing treatment reduced the H2O2 accumulated by French bean cells treated with C. lindemuthianum elicitor. Rose cells produced reductant capable of stimulating horseradish (Armoracia lapathifolia) peroxidase to form H2O2 but did not have a peroxidase capable of forming H2O2 in the presence of reductant. Rose and French bean cells thus appear to be responding by different mechanisms to generate the oxidative burst.