Arquitecto, obra y método. Análisis comparado de diferentes estrategias metodológicas singulares de la creación arquitectónica contemporánea

La tesis se acerca a la cuestión del método en arquitectura focalizando su análisis principalmente en 5 obras concretas del panorama contemporáneo, tratando de desvelar las motivaciones últimas que las constituyen. El objeto y la obra arquitectónica se consideran así los elementos susceptibles de ofrecer el conocimiento material que permite desentrañar el modo y la metodología por el que éstos han sido concebidos. Con esta particular aproximación se desafían los tradicionales estudios que han comprendido al método como una sistemática universal o reglada, ya que en este caso el propósito consiste más bien en esclarecer los fundamentos y principios que subyacen a cada objeto arquitectónico más específico. Este hecho ha llevado al índice de la tesis a ordenarse según una lista de arquitectos y obras que no presentan otra particularidad que la de ser recientes y muy diferentes entre sí. Esta aparente arbitrariedad con la que las obras se eligen cobra sentido a lo largo del propio análisis y recorrido, ya que éste apuesta por recabar el suficiente contenido que diversifique y singularice cada elección, de tal modo que ya en sí, ésta obtenga rango paradigmático. El hecho de que la tesis evite los planteamientos totalitarios no quiere decir que trate de promover el relativismo de las diferentes opciones, dado que más bien pretende analizar hasta el fondo las cuestiones últimas y lo más valioso que cada opción representa. Por su parte, la conclusión de la tesis muestra una complementariedad al carácter específico de los capítulos previos, ya que ésta se atreve a avanzar con cierta voluntad conclusiva hacia la definición de los principios más sustanciales del método en arquitectura hoy. Y precisamente, una de las claves esenciales que ha mostrado la arquitectura del hoy que nos toca vivir consiste en la condición más específica de la obra arquitectónica misma por encima de cualquier connotación genérica o transcendente que ésta pueda sugerir. De tal modo que los arquitectos elegidos, por muy diferentes que aparentan ser, han tomado como punto de partida la condición objetual de la obra más allá de cualquier contenido ideal que a ésta pueda adscribírsele. En todos los casos, si bien de diferente manera, la obra arquitectónica en sí misma se ha constituido en una nueva clave en la que tratan de dirimirse las eternas dialécticas entre el objeto y su significado, entre la estructura conceptual y su connotación simbólica. De este modo, las dialécticas relativas a la unidad de la diversidad, la identidad de la multiplicidad, la síntesis que pretende la integración de lo complejo, o en su caso, la indispensable sostenibilidad de lo inconsistente, se han convertido en la temática principal de este análisis que ha comprendido al método a partir la misma paradoja por la que la arquitectura actual se ve afectada. La misma dialéctica imposible de resolver que ha conducido a la arquitectura más reciente a decantarse a favor de la consustancialidad intrínseca a la misma obra. La tesis por su parte también se ha focalizado en la obra y en el hecho arquitectónico puntual, siguiendo la tendencia de la misma arquitectura que se analizaba. Sin embargo, sus extensos análisis nos han llevado a desentrañar el sentido y la posición estratégica personal que se encuentra más allá de la exacerbación inmanente de la arquitectura de estos últimos años. Es decir, tal y como se ha insistido, el análisis de la obra nos ha llevado, a través de la pregunta por su método, al arquitecto que la ha proyectado. Y así se ha creado este triángulo trinitario, ‘Arquitecto, obra y método’, tratando de preguntarse hasta el final por la singularidad más específica del método de cada arquitecto, en el convencimiento de que método y obra se vinculan precisamente ahí, en las experiencias personales singulares que han posibilitado las mayores creaciones y más sustantivas novedades. ABSTRACT This thesis approaches the question of Method in architecture by focusing its analysis on 5 specific contemporary projects, trying to unveil their underlying constitutive motivations. Thus, architectonical object and work are considered the susceptible elements that provide the material knowledge that unravels the manner and the methodology by which they were conceived. With this particular approach, this thesis challenges other traditional studies, those that regard the Method in architecture as a regulated or universal systematic procedure. On the contrary, the purpose of this thesis is to clarify the foundations and principles that underlie each specific architectonical object. This has led to the ordering of the thesis index according to a list of architects and projects that do not present any other distinctiveness except that they are recent and very different to each other. However, this apparent arbitrariness of the choice of each architect becomes meaningful during its analytical development. This analysis attempts to seek out the content which diversifies each choice enough, and makes it singular enough, so that each selected candidate acquires paradigmatic range by itself. Therefore, the fact that the thesis avoids totalitarian approaches does not lead it to promote the relativism of different choices, but rather the thesis tries to thoroughly analyze the most valuable achievements of each option. Nonetheless, the conclusion of the thesis shows a position that complements the specific character of the previous chapters, since it dares to move forward conclusively towards the definition of the essential principles of the Method in architecture today. Indeed, the thesis shows one of the basic keys of today’s architecture, which is the specific condition of the architectural work itself above any generic or transcendental connotation. Thus, the selected architects, however different they appear to be, take the objectual condition of the work (thingness) as their starting point beyond any ideal content that might be ascribed to their architecture. Although differently, in each case the architectonical work itself has become the new indispensable key to deal with the eternal dialectics between the object and its meaning, between conceptual structure and its symbolic connotation. Thus, the dialectics between unity and diversity, the identity of multiplicity, the synthesis that aims at the integration of complexity, or even, the indispensable consistency of the unavoidable inconsistency, have all become the main themes of this analysis, an analysis that understands the issue of Method from the same paradoxical situation of today’s architecture. This is the same paradox which has led the latest architecture to align itself with the intrinsic consubstantiality of the architectonical work itself. Therefore, the thesis is also focused on the Work, on the specific architectonical fact, following the course of the same architecture that it analyses. However, extensive analyses of the thesis have led us to clarify the sense and personal strategic position that is beyond the immanent exacerbation of architecture in recent years. Therefore, as stressed throughout the text, the analysis, that has explored the Method of architectonical Work, has led us to the architect who conceived it. Thus, this trinitarian triangle, ‘Architect, Work and Method’, has been created, attempting to explore in depth the specific uniqueness of each architect’s method, convinced that Method and Work do actually connect in the singular experiences that have achieved the greatest and most substantial creations.

Determination of Maximum Mechanical Energy Efficiency in Energy Galloping Systems

Transverse galloping is a type of aeroelastic instability characterized by large amplitude, low frequency, normal to wind oscillations that appear in some elastic two-dimensional bluff bodies when subjected to a fluid flow, provided that the flow velocity exceeds a threshold critical value. Such an oscillatory motion is explained because of the energy transfer from the flow to the two-dimensional bluff body. The 7 amount of energy that can be extracted depends on the cross section of the galloping prism. Assuming that the Glauert-Den Hartog quasistatic criterion for galloping instability is satisfied in a first approximation, the suitability of a given cross section for energy harvesting is evaluated by analyzing the lateral aerodynamic force coefficient, fitting a function with a power series in tan a (a being the angle of attack) to 10 available experimental data. In this paper, a fairly large number of simple prisms (triangle, ellipse, biconvex, and rhombus cross sections, as well 11 as D-shaped bodies) is analyzed for suitability as energy harvesters. The influence of the fitting process in the energy harvesting efficiency evaluation is also demonstrated. The analysis shows that the more promising bodies are those with isosceles or approximate isosceles cross sections.

Caracterización multivariada de los temporales para su aplicación en el dimensionamiento del manto principal

En esta tesis se presenta una metodología para la caracterización del oleaje, dentro del marco de las nuevas Recomendaciones para Obras Marítimas (ROM 0.0.-00 y ROM 1.0-09), por ser una de las principales acciones que afectan a la estabilidad de las estructuras marítimas. Debido al carácter aleatorio intrínsecamente multivariado de la acción considerada, las tormentas, su caracterización paramétrica se realiza en términos de funciones cópula uniparamétricas. Las variables consideradas son altura de ola significante del pico de la tormenta, el periodo medio asociado y la magnitud, o número de olas, de todo el ciclo de solicitación. Para establecer un patrón teórico de evolución de la tormenta que permita extrapolar las muestras fuera de la región con datos se analizan los modelos teóricos existentes, comprobándose que no reproducen adecuadamente las tormentas constituidas por estados de mar con un peso importante de oleaje swell. Para evitar esta limitación se proponen cuatro modelos teóricos de evolución de tormentas con distintas formas geométricas. El análisis de los modelos existentes y los propuestos pone de relieve que el Modelo Magnitud Equivalente de Tormenta (EMS= Equivalent Magnitude Storm) con la forma triangular es el que mejor adapta las tormentas constituidas por estados de mar típicos del viento. Para tormentas con un mayor grado de desarrollo, el modelo teórico de tormenta EMS con la forma trapezoidal es el adecuado. De las aproximaciones propuestas para establecer el periodo medio de los sucesivos estados de mar del ciclo de solicitación. la propuesta por Martín Soldevilla et al., (2009) es la más versátil y , en general , mejor reproduce la evolución de todo tipo de tormentas. La caracterización de las tormentas se complementa con la altura de ola máxima. Debido a la mayor disponibilidad y longitud temporal de los datos sintéticos frente a las registros, la práctica totalidad de los análisis de extremos se realizan con tormentas sintéticas en las que la distribución de olas individuales es desconocida. Para evitar esta limitación se utilizan modelos teóricos de distribución de olas acordes a las características de cada uno de los estados de mar que conforman la tormenta sintética. Para establecer dichas características se utiliza la curtosis y en función de su valor la altura de ola máxima se determina asumiendo una determinada distribución de olas. Para estados de mar lineales la distribución de olas individuales de Rayleigh es la considerada. Para condiciones no lineales de gran ancho de banda el modelo de distribución de olas propuesto por Dawson, (2004) es el utilizado y si es de banda estrecha las predicciones de (Boccotti, (1989), Boccotti et al., (2013)) se compara con las resultantes del modelo de Dawson. La caracterización de la evolución de las tormentas en términos multivariados es aplicada al estudio de la progresión del daño del manto principal de diques en talud, y al rebase de las olas. Ambos aspectos cubren el segundo objetivo de la tesis en el que se propone una nueva formulación para el dimensionamiento de mantos constituidos por bloques cúbicos de hormigón. Para el desarrollo de esta nueva formulación se han utilizado los resultados recogidos en los estudios de estabilidad del manto principal de diques talud realizados en modelo físico a escala reducida en el Centro de Estudios de Puertos y Costas (CEDEX) desde la década de los 80 empleando, en su mayoría, bloques paralelepípedos cúbicos de hormigón. Por este motivo y porque los últimos diques construidos en la costa Española utilizan este tipo de pieza, es por lo que la formulación planteada se centra en este tipo de pieza. Después de un primer análisis de las fórmulas de cálculo y de evolución existentes, se llega a la conclusión de que es necesario realizar un esfuerzo de investigación en este campo, así como ensayos en laboratorio y recogida de datos in-situ con base a desarrollar fórmulas de evolución de daño para mantos constituidos por piezas diferentes a la escollera, que tenga en cuenta las principales variables que condiciona su estabilidad. En esta parte de la tesis se propone un método de análisis de evolución de daño, que incluye el criterio de inicio de avería, adecuada para diques en talud constituidos por bloque cúbicos de hormigón y que considera la incidencia oblicua, el daño acumulado y el rebase. This thesis proposes a methodology to estimate sea waves, one of the main actions affecting the maritime structures stability, complying with (ROM 0.0.-00 & ROM 1.0-09.Due to the multivariate behavior of sea storms, the characterization of the structures of sea storms is done using copula function. The analyzed variables are the significant height wave, mean period and magnitude or number of waves during the storm history. The storm evolution in terms of the significant height wave and the mean period is also studied in other to analyze the progressive failure modes. The existing models of evolution are studied, verifying that these approximations do not adjust accurately for developed waves. To overcome this disadvantage, four evolution models are proposed, with some geometrical shapes associated to fit any development degree. The proposed Equivalent Magnitude Storm model, EMS, generally obtains the best results for any kind of storm (predominant sea, swell or both). The triangle is recommended for typical sea storms whereas the trapezoid shape is much more appropriate for more developed storm conditions.The Martín Soldevilla et al., (2009) approach to estimate the mean period is better than others approaches used.The storm characterization is completed with the maximum wave height of the whole storm history. Due to synthetic historical waves databases are more accessible and longer than recorded database, the extreme analyses are done with synthetic data. For this reason the individual waves’ distribution is not known. For that limitation to be avoided, and depending on the characteristics of every sea states, one theoretical model of waves is choose and used. The kurtosis parameter is used to distinguish between linear and nonlinear sea states. The Rayleigh model is used for the linear sea states. For the nonlinear sea states, Dawson, (2004) approach is used for non-narrow bandwidth storms, comparing the results with the Boccotti, (1989), Boccotti et al., (2013) approach, with is used for narrow bandwidth storms. The multivariate and storm evolution characterization is used to analyze of stone armour damage progression and wave overtopping discharge. Both aspects are included in the second part of the thesis, with a new formula is proposed to design cubes armour layer. The results the stability studies of armour layer, done in the Centre for Harbours and Coastal Studies (CEDEX) laboratory are used for defining a new stability formula. For this reason and because the last biggest breakwater built in Spain using the cube, the damage progression is analyze for this kind of concrete block. Before to analyze the existing formulae, it is concluded that it is necessary more investigation, more tests in laboratory and data gathering in situ to define damage evolution formulae to armour of other kind of pieces and that takes to account the principal variables. This thesis proposed a method to calculate the damage progression including oblique waves, accumulated damage, and overtopping effect. The method also takes account the beginning of the movement of the blocks.

Multiscale modeling of the plastic behaviour in single crystal tungsten: from atomistic to crystal plasticity simulations

En una planta de fusión, los materiales en contacto con el plasma así como los materiales de primera pared experimentan condiciones particularmente hostiles al estar expuestos a altos flujos de partículas, neutrones y grandes cargas térmicas. Como consecuencia de estas diferentes y complejas condiciones de trabajo, el estudio, desarrollo y diseño de estos materiales es uno de los más importantes retos que ha surgido en los últimos años para la comunidad científica en el campo de los materiales y la energía. Debido a su baja tasa de erosión, alta resistencia al sputtering, alta conductividad térmica, muy alto punto de fusión y baja retención de tritio, el tungsteno (wolframio) es un importante candidato como material de primera pared y como posible material estructural avanzado en fusión por confinamiento magnético e inercial. Sin embargo, el tiempo de vida del tungsteno viene controlado por diversos factores como son su respuesta termo-mecánica en la superficie, la posibilidad de fusión y el fallo por acumulación de helio. Es por ello que el tiempo de vida limitado por la respuesta mecánica del tungsteno (W), y en particular su fragilidad, sean dos importantes aspectos que tienes que ser investigados. El comportamiento plástico en materiales refractarios con estructura cristalina cúbica centrada en las caras (bcc) como el tungsteno está gobernado por las dislocaciones de tipo tornillo a escala atómica y por conjuntos e interacciones de dislocaciones a escalas más grandes. El modelado de este complejo comportamiento requiere la aplicación de métodos capaces de resolver de forma rigurosa cada una de las escalas. El trabajo que se presenta en esta tesis propone un modelado multiescala que es capaz de dar respuestas ingenieriles a las solicitudes técnicas del tungsteno, y que a su vez está apoyado por la rigurosa física subyacente a extensas simulaciones atomísticas. En primer lugar, las propiedades estáticas y dinámicas de las dislocaciones de tipo tornillo en cinco potenciales interatómicos de tungsteno son comparadas, determinando cuáles de ellos garantizan una mayor fidelidad física y eficiencia computacional. Las grandes tasas de deformación asociadas a las técnicas de dinámica molecular hacen que las funciones de movilidad de las dislocaciones obtenidas no puedan ser utilizadas en los siguientes pasos del modelado multiescala. En este trabajo, proponemos dos métodos alternativos para obtener las funciones de movilidad de las dislocaciones: un modelo Monte Cario cinético y expresiones analíticas. El conjunto de parámetros necesarios para formular el modelo de Monte Cario cinético y la ley de movilidad analítica son calculados atomísticamente. Estos parámetros incluyen, pero no se limitan a: la determinación de las entalpias y energías de formación de las parejas de escalones que forman las dislocaciones, la parametrización de los efectos de no Schmid característicos en materiales bcc,etc. Conociendo la ley de movilidad de las dislocaciones en función del esfuerzo aplicado y la temperatura, se introduce esta relación como ecuación de flujo dentro de un modelo de plasticidad cristalina. La predicción del modelo sobre la dependencia del límite de fluencia con la temperatura es validada experimentalmente con ensayos uniaxiales en tungsteno monocristalino. A continuación, se calcula el límite de fluencia al aplicar ensayos uniaxiales de tensión para un conjunto de orientaciones cristalográticas dentro del triángulo estándar variando la tasa de deformación y la temperatura de los ensayos. Finalmente, y con el objetivo de ser capaces de predecir una respuesta más dúctil del tungsteno para una variedad de estados de carga, se realizan ensayos biaxiales de tensión sobre algunas de las orientaciones cristalográficas ya estudiadas en función de la temperatura.-------------------------------------------------------------------------ABSTRACT ----------------------------------------------------------Tungsten and tungsten alloys are being considered as leading candidates for structural and functional materials in future fusion energy devices. The most attractive properties of tungsten for the design of magnetic and inertial fusion energy reactors are its high melting point, high thermal conductivity, low sputtering yield and low longterm disposal radioactive footprint. However, tungsten also presents a very low fracture toughness, mostly associated with inter-granular failure and bulk plasticity, that limits its applications. As a result of these various and complex conditions of work, the study, development and design of these materials is one of the most important challenges that have emerged in recent years to the scientific community in the field of materials for energy applications. The plastic behavior of body-centered cubic (bcc) refractory metals like tungsten is governed by the kink-pair mediated thermally activated motion of h¿ (\1 11)i screw dislocations on the atomistic scale and by ensembles and interactions of dislocations at larger scales. Modeling this complex behavior requires the application of methods capable of resolving rigorously each relevant scale. The work presented in this thesis proposes a multiscale model approach that gives engineering-level responses to the technical specifications required for the use of tungsten in fusion energy reactors, and it is also supported by the rigorous underlying physics of extensive atomistic simulations. First, the static and dynamic properties of screw dislocations in five interatomic potentials for tungsten are compared, determining which of these ensure greater physical fidelity and computational efficiency. The large strain rates associated with molecular dynamics techniques make the dislocation mobility functions obtained not suitable to be used in the next steps of the multiscale model. Therefore, it is necessary to employ mobility laws obtained from a different method. In this work, we suggest two alternative methods to get the dislocation mobility functions: a kinetic Monte Carlo model and analytical expressions. The set of parameters needed to formulate the kinetic Monte Carlo model and the analytical mobility law are calculated atomistically. These parameters include, but are not limited to: enthalpy and energy barriers of kink-pairs as a function of the stress, width of the kink-pairs, non-Schmid effects ( both twinning-antitwinning asymmetry and non-glide stresses), etc. The function relating dislocation velocity with applied stress and temperature is used as the main source of constitutive information into a dislocation-based crystal plasticity framework. We validate the dependence of the yield strength with the temperature predicted by the model against existing experimental data of tensile tests in singlecrystal tungsten, with excellent agreement between the simulations and the measured data. We then extend the model to a number of crystallographic orientations uniformly distributed in the standard triangle and study the effects of temperature and strain rate. Finally, we perform biaxial tensile tests and provide the yield surface as a function of the temperature for some of the crystallographic orientations explored in the uniaxial tensile tests.

Arquitecto, obra y método. Análisis comparado de diferentes estrategias metodológicas singulares de la creación arquitectónica contemporánea

La tesis se acerca a la cuestión del método en arquitectura focalizando su análisis principalmente en 5 obras concretas del panorama contemporáneo, tratando de desvelar las motivaciones últimas que las constituyen. El objeto y la obra arquitectónica se consideran así los elementos susceptibles de ofrecer el conocimiento material que permite desentrañar el modo y la metodología por el que éstos han sido concebidos. Con esta particular aproximación se desafían los tradicionales estudios que han comprendido al método como una sistemática universal o reglada, ya que en este caso el propósito consiste más bien en esclarecer los fundamentos y principios que subyacen a cada objeto arquitectónico más específico. Este hecho ha llevado al índice de la tesis a ordenarse según una lista de arquitectos y obras que no presentan otra particularidad que la de ser recientes y muy diferentes entre sí. Esta aparente arbitrariedad con la que las obras se eligen cobra sentido a lo largo del propio análisis y recorrido, ya que éste apuesta por recabar el suficiente contenido que diversifique y singularice cada elección, de tal modo que ya en sí, ésta obtenga rango paradigmático. El hecho de que la tesis evite los planteamientos totalitarios no quiere decir que trate de promover el relativismo de las diferentes opciones, dado que más bien pretende analizar hasta el fondo las cuestiones últimas y lo más valioso que cada opción representa. Por su parte, la conclusión de la tesis muestra una complementariedad al carácter específico de los capítulos previos, ya que ésta se atreve a avanzar con cierta voluntad conclusiva hacia la definición de los principios más sustanciales del método en arquitectura hoy. Y precisamente, una de las claves esenciales que ha mostrado la arquitectura del hoy que nos toca vivir consiste en la condición más específica de la obra arquitectónica misma por encima de cualquier connotación genérica o transcendente que ésta pueda sugerir. De tal modo que los arquitectos elegidos, por muy diferentes que aparentan ser, han tomado como punto de partida la condición objetual de la obra más allá de cualquier contenido ideal que a ésta pueda adscribírsele. En todos los casos, si bien de diferente manera, la obra arquitectónica en sí misma se ha constituido en una nueva clave en la que tratan de dirimirse las eternas dialécticas entre el objeto y su significado, entre la estructura conceptual y su connotación simbólica. De este modo, las dialécticas relativas a la unidad de la diversidad, la identidad de la multiplicidad, la síntesis que pretende la integración de lo complejo, o en su caso, la indispensable sostenibilidad de lo inconsistente, se han convertido en la temática principal de este análisis que ha comprendido al método a partir la misma paradoja por la que la arquitectura actual se ve afectada. La misma dialéctica imposible de resolver que ha conducido a la arquitectura más reciente a decantarse a favor de la consustancialidad intrínseca a la misma obra. La tesis por su parte también se ha focalizado en la obra y en el hecho arquitectónico puntual, siguiendo la tendencia de la misma arquitectura que se analizaba. Sin embargo, sus extensos análisis nos han llevado a desentrañar el sentido y la posición estratégica personal que se encuentra más allá de la exacerbación inmanente de la arquitectura de estos últimos años. Es decir, tal y como se ha insistido, el análisis de la obra nos ha llevado, a través de la pregunta por su método, al arquitecto que la ha proyectado. Y así se ha creado este triángulo trinitario, ‘Arquitecto, obra y método’, tratando de preguntarse hasta el final por la singularidad más específica del método de cada arquitecto, en el convencimiento de que método y obra se vinculan precisamente ahí, en las experiencias personales singulares que han posibilitado las mayores creaciones y más sustantivas novedades. ABSTRACT This thesis approaches the question of Method in architecture by focusing its analysis on 5 specific contemporary projects, trying to unveil their underlying constitutive motivations. Thus, architectonical object and work are considered the susceptible elements that provide the material knowledge that unravels the manner and the methodology by which they were conceived. With this particular approach, this thesis challenges other traditional studies, those that regard the Method in architecture as a regulated or universal systematic procedure. On the contrary, the purpose of this thesis is to clarify the foundations and principles that underlie each specific architectonical object. This has led to the ordering of the thesis index according to a list of architects and projects that do not present any other distinctiveness except that they are recent and very different to each other. However, this apparent arbitrariness of the choice of each architect becomes meaningful during its analytical development. This analysis attempts to seek out the content which diversifies each choice enough, and makes it singular enough, so that each selected candidate acquires paradigmatic range by itself. Therefore, the fact that the thesis avoids totalitarian approaches does not lead it to promote the relativism of different choices, but rather the thesis tries to thoroughly analyze the most valuable achievements of each option. Nonetheless, the conclusion of the thesis shows a position that complements the specific character of the previous chapters, since it dares to move forward conclusively towards the definition of the essential principles of the Method in architecture today. Indeed, the thesis shows one of the basic keys of today’s architecture, which is the specific condition of the architectural work itself above any generic or transcendental connotation. Thus, the selected architects, however different they appear to be, take the objectual condition of the work (thingness) as their starting point beyond any ideal content that might be ascribed to their architecture. Although differently, in each case the architectonical work itself has become the new indispensable key to deal with the eternal dialectics between the object and its meaning, between conceptual structure and its symbolic connotation. Thus, the dialectics between unity and diversity, the identity of multiplicity, the synthesis that aims at the integration of complexity, or even, the indispensable consistency of the unavoidable inconsistency, have all become the main themes of this analysis, an analysis that understands the issue of Method from the same paradoxical situation of today’s architecture. This is the same paradox which has led the latest architecture to align itself with the intrinsic consubstantiality of the architectonical work itself. Therefore, the thesis is also focused on the Work, on the specific architectonical fact, following the course of the same architecture that it analyses. However, extensive analyses of the thesis have led us to clarify the sense and personal strategic position that is beyond the immanent exacerbation of architecture in recent years. Therefore, as stressed throughout the text, the analysis, that has explored the Method of architectonical Work, has led us to the architect who conceived it. Thus, this trinitarian triangle, ‘Architect, Work and Method’, has been created, attempting to explore in depth the specific uniqueness of each architect’s method, convinced that Method and Work do actually connect in the singular experiences that have achieved the greatest and most substantial creations.

Selection, training and validation process of a sensory panel for bread analysis: Influence of cultivar on the quality of breads made from common wheat and spelta wheat

A protocol of selection, training and validation of the members of the panel for bread sensory analysis is proposed to assess the influence of wheat cultivar on the sensory quality of bread. Three cultivars of bread wheat and two cultivars of spelt wheat organically-grown under the same edaphoclimatic conditions were milled and baked using the same milling and baking procedure. Through the use of triangle tests, differences were identified between the five breads. Significant differences were found between the spelt breads and those made with bread wheat for the attributes ?crumb cell homogeneity? and ?crumb elasticity?. Significant differences were also found for the odor and flavor attributes, with the bread made with ?Espelta Navarra? being the most complex, from a sensory point of view. Based on the results of this study, we propose that sensory properties should be considered as breeding criteria for future work on genetic improvement.

Haus des Rundfunks en Berlin, de Hans Poelzig, 1930

Poelzig affirmed that the technical ideal consisted of using the least amount of shapes and materials possible. He understood architecture as an art, as the expression of form; In contrast to the indifference of the most widely recognized values of modernism: technical innovation in construction along with the functionality of buildings. The Haus des Rundfunks is a powerful hermetic factory in the shape of a triangle and of uniform height. Mass, geometric rigidity and the absence of textures make up a building which relinquishes expressing the new media, with a perimeter ring consisting of offices and larger spaces that are more characteristic towards their inside. Geometry is used as a means to organize and limit the shapes of the buildings. The arrangement and division of different intercommunicating spaces are subjected to the crudeness of their geometric limits. Interior spaces ranging from the most static to the most dynamic are defined through the qualities of materials and geometries. This turns the spaces into geometric grids which decompose the wall, floor and ceiling planes into fragments, regardless of the constructive reality provided by a metallic frame structure. Its polyhedral lamps enclose artificial and emphasize the value of the grid. Poelzig limits himself to dealing with planes within a closed volume of uniform height, and maintains geometric rigidity compatible with a certain degree of figurativeness. This article tries to bring to life this exemplary building, marked by its passivity and hermetism.

Impact of using new commercial glutathione enriched inactive dry yeast oenological preparations on the aroma and sensory properties of wines

The effect of the addition of a commercial enriched glutathione inactive dry yeast oenological preparation in the volatile and sensory properties of industrially manufactured rosé Grenache wines was evaluated during their shelf-life. In addition, triangle tests were performed at different times during wine aging (among 1 and 9 months) to determine the sensory differences between wines with and without glutathione inactive dry yeast preparations. Descriptive sensory analysis with a trained panel was carried out when sensory differences in the triangle test were noticed. In addition, consumer tests were performed in order to investigate consumers’ acceptability of wines. Results revealed significant sensory differences between control and glutathione inactive dry yeast wines after 9 months of aging. At that time, glutathione inactive dry yeast wines were more intense in fruity aromas (strawberry, banana) and less intense in yeast notes than control wine. The impact of the glutathione inactive dry yeast in the aroma might be the consequence of different effects that these preparations could induce in wine composition: modification of yeast byproducts during fermentation, release of volatile compounds from inactive dry yeast, interaction of wine volatile compounds with yeast macromolecules from inactive dry yeast and a possible antioxidant effect of the glutathione released by the inactive dry yeast preparation on some specific volatile compounds.

Improvement of theoretical storm characterization for different climate conditions

The different theoretical models related with storm wave characterization focus on determining the significant wave height of the peak storm, the mean period and, usually assuming a triangle storm shape, their duration. In some cases, the main direction is also considered. Nevertheless, definition of the whole storm history, including the variation of the main random variables during the storm cycle is not taken into consideration. The representativeness of the proposed storm models, analysed in a recent study using an empirical maximum energy flux time dependent function shows that the behaviour of the different storm models is extremely dependent on the climatic characteristics of the project area. Moreover, there are no theoretical models able to adequately reproduce storm history evolution of the sea states characterized by important swell components. To overcome this shortcoming, several theoretical storm shapes are investigated taking into consideration the bases of the three best theoretical storm models, the Equivalent Magnitude Storm (EMS), the Equivalent Number of Waves Storm (ENWS) and the Equivalent Duration Storm (EDS) models. To analyse the representativeness of the new storm shape, the aforementioned maximum energy flux formulation and a wave overtopping discharge structure function are used. With the empirical energy flux formulation, correctness of the different approaches is focussed on the progressive hydraulic stability loss of the main armour layer caused by real and theoretical storms. For the overtopping structure equation, the total volume of discharge is considered. In all cases, the results obtained highlight the greater representativeness of the triangular EMS model for sea waves and the trapezoidal (nonparallel sides) EMS model for waves with a higher degree of wave development. Taking into account the increase in offshore and shallow water wind turbines, maritime transport and deep vertical breakwaters, the maximum wave height of the whole storm history and that corresponding to each sea state belonging to its cycle's evolution is also considered. The procedure considers the information usually available for extreme waves' characterization. Extrapolations of the maximum wave height of the selected storms have also been considered. The 4th order statistics of the sea state belonging to the real and theoretical storm have been estimated to complete the statistical analysis of individual wave height

Hacia una arquitectura de procesos : transformaciones disciplinares a través de la Computación en los modelos de Alexander, Negroponte y Price

La premisa inicial de la tesis examina cómo las secuelas de Segunda Guerra mundial motivaron una revisión general de la Ciencia y procuraron una nueva relación entre el hombre y su entorno. Matemáticas, Física y Biología gestaron las Ciencias de la Computación como disciplina de convergencia. En un momento de re-definición del objeto científico, una serie de arquitectos vislumbraron la oportunidad para transformar ciertas convenciones disciplinares. Mediante la incorporación de ontologías y procedimientos de cibernética y computación, trazaron un nuevo espacio arquitectónico. Legitimados por un despegue tecnológico incuestionable, desafían los límites de la profesión explorando campos abiertos a nuevos programas y acciones; amplían el dominio natural de la Arquitectura más allá del objeto(terminado) hacia el proceso(abierto). Se da inicio a la tesis describiendo los antecedentes que conducen a ese escenario de cambio. Se anotan aspectos de Teoría de Sistemas, Computación, Biología y de ciertos referentes de Arquitectura con relevancia para esa nuevo planteamiento. En esos antecedentes residen los argumentos para orientar la disciplina hacia el trabajo con procesos. La linea argumental central del texto aborda la obra de Christopher Alexander, Nicholas Negroponte y Cedric Price a través de una producción teórica y práctica transformada por la computación, y examina la contribución conceptual de cada autor. El análisis comparado de sus modelos se dispone mediante la disección de tres conceptos convergentes: Sistema, Código y Proceso. La discusión crítica se articula por una triangulación entre los autores, donde se identifican comparando por pares las coincidencias y controversias entre ellos. Sirve este procedimiento al propósito de tender un puente conceptual con el escenario arquitectónico actual estimando el impacto de sus propuestas. Se valora su contribución en la deriva del programa cerrado a la especulación , de lo formal a lo informal, de lo único a lo múltiple; del estudio de arquitectura al laboratorio de investigación. Para guiar ese recorrido por la significación de cada autor en el desarrollo digital de la disciplina, se incorporan a la escena dos predicados esenciales; expertos en computación que trabajaron de enlace entre los autores, matizando el significado de sus modelos. El trabajo de Gordon Pask y John Frazer constituye el vehículo de transmisión de los hallazgos de aquellos años, prolonga los caminos iniciados entonces, en la arquitectura de hoy y la que ya se está diseñando para mañana. ABSTRACT The initial premise of the thesis examines how the aftermath of second world war motivated a general revision of science and procure the basis of a new relation between mankind and its environment. Mathematics, Physics, and Biology gave birth to the Computer Sciences as a blend of different knowledge and procedures. In a time when the object of major sciences was being redefined, a few architects saw a promising opportunity for transforming the Architectural convention. By implementing the concepts, ontology and procedures of Cybernetics, Artificial Intelligence and Information Technology, they envisioned a new space for their discipline. In the verge of transgression three prescient architects proposed complete architectural systems through their writings and projects; New systems that challenged the profession exploring open fields through program and action, questioning the culture of conservatism; They shifted architectural endeavor from object to process. The thesis starts describing the scientific and architectural background that lead to that opportunity, annotating aspects of Systems Theory, Computing, Biology and previous Architecture form the process perspective. It then focuses on the Works of Christopher Alexander, Nicholas Negroponte and Cedric Price through their work, and examines each authors conceptual contribution. It proceeds to a critical analysis of their proposals on three key converging aspects: system, architectural encoding and process. Finally, the thesis provides a comparative discussion between the three authors, and unfolds the impact of their work in todays architectural scenario. Their contribution to shift from service to speculation, from formal to informal , from unitary to multiple; from orthodox architecture studio to open laboratories of praxis through research. In order to conclude that triangle of concepts, other contributions come into scene to provide relevant predicates and complete those models. A reference to Gordon Pask and John Frazer is then provided with particular interest in their role as link between those pioneers and todays perspective, pushing the boundaries of both what architecture was and what it could become.

The use of site-directed fluorophore labeling and donor–donor energy migration to investigate solution structure and dynamics in proteins

The use of molecular genetics for introducing fluorescent molecules enables the use of donor–donor energy migration to determine intramolecular distances in a variety of proteins. This approach can be applied to examine the overall molecular dimensions of proteins and to investigate structural changes upon interactions with specific target molecules. In this report, the donor–donor energy migration method is demonstrated by experiments with the latent form of plasminogen activator inhibitor type 1. Based on the known x-ray structure of plasminogen activator inhibitor type 1, three positions forming the corners of a triangle were chosen. Double Cys substitution mutants (V106C-H185C, H185C-M266C, and M266C-V106C) and corresponding single substitution mutants (V106C, H185C, and M266C) were created and labeled with a sulfhydryl specific derivative of BODIPY (=the D molecule). The side lengths of this triangle were obtained from analyses of the experimental data. The analyses account for the local anisotropic order and rotational motions of the D molecules, as well as for the influence of a partial DD-labeling. The distances, as determined from x-ray diffraction, between the Cα-atoms of the positions V106C–H185C, H185C–M266C, and M266C–V106C were 60.9, 30.8, and 55.1 Å, respectively. These are in good agreement with the distances of 54 ± 4, 38 ± 3, and 55 ± 3 Å, as determined between the BODIPY groups attached via linkers to the same residues. Although the positions of the D-molecules and the Cα-atoms physically cannot coincide, there is a reasonable agreement between the methods.

Silver-based crystalline nanoparticles, microbially fabricated

One mechanism of silver resistance in microorganisms is accumulation of the metal ions in the cell. Here, we report on the phenomenon of biosynthesis of silver-based single crystals with well-defined compositions and shapes, such as equilateral triangles and hexagons, in Pseudomonas stutzeri AG259. The crystals were up to 200 nm in size and were often located at the cell poles. Transmission electron microscopy, quantitative energy-dispersive x-ray analysis, and electron diffraction established that the crystals comprise at least three different types, found both in whole cells and thin sections. These Ag-containing crystals are embedded in the organic matrix of the bacteria. Their possible potential as organic-metal composites in thin film and surface coating technology is discussed.

Preassembly of interleukin 2 (IL-2) receptor subunits on resting Kit 225 K6 T cells and their modulation by IL-2, IL-7, and IL-15: A fluorescence resonance energy transfer study

Assembly and mutual proximities of α, β, and γc subunits of the interleukin 2 receptors (IL-2R) in plasma membranes of Kit 225 K6 T lymphoma cells were investigated by fluorescence resonance energy transfer (FRET) using fluorescein isothiocyanate- and Cy3-conjugated monoclonal antibodies (mAbs) that were directed against the IL-2Rα, IL-2Rβ, and γc subunits of IL-2R. The cell-surface distribution of subunits was analyzed at the nanometer scale (2–10 nm) by FRET on a cell-by-cell basis. The cells were probed in resting phase and after coculture with saturating concentrations of IL-2, IL-7, and IL-15. FRET data from donor- and acceptor-labeled IL-2Rβ-α, γ-α, and γ-β pairs demonstrated close proximity of all subunits to each other in the plasma membrane of resting T cells. These mutual proximities do not appear to represent mAb-induced microaggregation, because FRET measurements with Fab fragments of the mAbs gave similar results. The relative proximities were meaningfully modulated by binding of IL-2, IL-7, and IL-15. Based on FRET analysis the topology of the three subunits at the surface of resting cells can be best described by a “triangular model” in the absence of added interleukins. IL-2 strengthens the bridges between the subunits, making the triangle more compact. IL-7 and IL-15 act in the opposite direction by opening the triangle possibly because they associate their private specific α receptors with the β and/or γc subunits of the IL-2R complex. These data suggest that IL-2R subunits are already colocalized in resting T cells and do not require cytokine-induced redistribution. This colocalization is significantly modulated by binding of relevant interleukins in a cytokine-specific manner.

Pentameric assembly of a neuronal glutamate transporter

Freeze-fracture electron microscopy was used to study the structure of a human neuronal glutamate transporter (EAAT3). EAAT3 was expressed in Xenopus laevis oocytes, and its function was correlated with the total number of transporters in the plasma membrane of the same cells. Function was assayed as the maximum charge moved in response to a series of transmembrane voltage pulses. The number of transporters in the plasma membrane was determined from the density of a distinct 10-nm freeze-fracture particle, which appeared in the protoplasmic face only after EAAT3 expression. The linear correlation between EAAT3 maximum carrier-mediated charge and the total number of the 10-nm particles suggested that this particle represented functional EAAT3 in the plasma membrane. The cross-sectional area of EAAT3 in the plasma membrane (48 ± 5 nm2) predicted 35 ± 3 transmembrane α-helices in the transporter complex. This information along with secondary structure models (6–10 transmembrane α-helices) suggested an oligomeric state for EAAT3. EAAT3 particles were pentagonal in shape in which five domains could be identified. They exhibited fivefold symmetry because they appeared as equilateral pentagons and the angle at the vertices was 110°. Each domain appeared to contribute to an extracellular mass that projects ≈3 nm into the extracellular space. Projections from all five domains taper toward an axis passing through the center of the pentagon, giving the transporter complex the appearance of a penton-based pyramid. The pentameric structure of EAAT3 offers new insights into its function as both a glutamate transporter and a glutamate-gated chloride channel.