Comparison of the defensive elicitation activity of cerato-populin and cerato-platanin: a structural model

Plants can defend themselves from potential pathogenic microorganisms relying on a complex interplay of signaling pathways: activation of the MAPK cascade, transcription of defense related genes, production of reactive oxygen species, nitric oxide and synthesis of other defensive compounds such as phytoalexins. These events are triggered by the recognition of pathogen’s effectors (effector-triggered immunity) or PAMPs (PAMP-triggered immunity). The Cerato Platanin Family (CPF) members are Cys-rich proteins secreted and localized on fungal cell walls, involved in several aspects of fungal development and pathogen-host interactions. Although more than hundred genes of the CPF have been identified and analyzed, the structural and functional characterization of the expressed proteins has been restricted only to few members of the family. Interestingly, those proteins have been shown to bind chitin with diverse affinity and after foliar treatment they elicit defensive mechanisms in host and non-host plants. This property turns cerato platanins into interesting candidates, worth to be studied to develop new fungal elicitors with applications in sustainable agriculture. This study focus on cerato-platanin (CP), core member of the family and on the orthologous cerato-populin (Pop1). The latter shows an identity of 62% and an overall homology of 73% with respect to CP. Both proteins are able to induce MAPKs phosphorylation, production of reactive oxygen species and nitric oxide, overexpression of defense’s related genes, programmed cell death and synthesis of phytoalexins. CP, however, when compared to Pop1, induces a faster response and, in some cases, a stronger activity on plane leaves. Aim of the present research is to verify if the dissimilarities observed in the defense elicitation activity of these proteins can be associated to their structural and dynamic features. Taking advantage of the available CP NMR structure, Pop1’s 3D one was obtained by homology modeling. Experimental residual dipolar couplings and 1H, 15N, 13C resonance assignments were used to validate the model. Previous works on CPF members, addressed the highly conserved random coil regions (loops b1-b2 and b2-b3) as sufficient and necessary to induce necrosis in plants’ leaves: that region was investigated in both Pop1 and CP. In the two proteins the loops differ, in their primary sequence, for few mutations and an insertion with a consequent diversification of the proteins’ electrostatic surface. A set of 2D and 3D NMR experiments was performed to characterize both the spatial arrangement and the dynamic features of the loops. NOE data revealed a more extended network of interactions between the loops in Pop1 than in CP. In addition, in Pop1 we identified a salt bridge Lys25/Asp52 and a strong hydrophobic interaction between Phe26/Trp53. These structural features were expected not only to affect the loops’ spatial arrangement, but also to reduce the degree of their conformational freedom. Relaxation data and the order parameter S2 indeed highlighted reduced flexibility, in particular for loop b1-b2 of Pop1. In vitro NMR experiments, where Pop1 and CP were titrated with oligosaccharides, supported the hypothesis that the loops structural and dynamic differences may be responsible for the different chitin-binding properties of the two proteins: CP selectively binds tetramers of chitin in a shallow groove on one side of the barrel defined by loops b1-b2, b2-b3 and b4-b5, Pop1, instead, interacts in a non-specific fashion with oligosaccharides. Because the region involved in chitin-binding is also responsible for the defense elicitation activity, possibly being recognized by plant's receptors, it is reasonable to expect that those structural and dynamic modifications may also justify the different extent of defense elicitation. To test that hypothesis, the initial steps of a protocol aimed to the identify a receptor for CP, in silico, are presented.

The Clinical Utility of the Adult Attachment Projective Picture System: A Clinician's Perspective

The Adult Attachment Projective Picture System (AAP) is the first performance- based measure of adult attachment to be developed. The purpose of the measure is to provide a clinical understanding of an adult client's attachment status and associated coping mechanisms. The AAP is a relatively new measure that has yet to be examined from a utility perspective. In the current study, seven psychologists completed a structured survey in order to identify their perspectives of the AAP and its utility as a clinical instrument. A phenomenological qualitative analysis of the data was conducted to derive themes about the AAP and its clinical utility. Analyses aimed to answer the following: What clinical considerations do clinician's focus on when deciding to use this measure? What are common factors among clinician's who do use the measure as well as those who do not? What aspects of the measure are user-friendly and what aspects are difficult? General themes that emerged include (a) the clinical information provided by the AAP is viewed by those who use it as unique and beneficial; (b) time commitment and cost for the clinician are common considerations when clinician's are deciding whether or not to use the AAP or when pursuing training; (c) the AAP provides an increased understanding of one's relational capacities and defenses; and (d) the coding system and transcription process are difficult aspects of the AAP and influence how and/or when it is used. In addition to these themes, multiple respondents discussed potential changes for the AAP that would increase their future use of the instrument. Finally, the implications of these results are discussed.

Male/Female Manifestations of Narcissistic Personality and Behavioral Disorders.

Specifically, this paper will address the following topics : 1. The history of psychoanalytic thinking onnarcissism will be discussed, leading up to more recent ideas on the narcissistic personality disorder. 2. Drawing on historical and current ideas, an integrated definition of the narcissistic personality disorder will be presented and elaborated upon, including an examination of differing male/female narcissistic compensatory styles. 3. To foster an understanding of the development of narcissistic defenses and of differing gender styles of defense, various theories relating to gender differences in the narcissistic personality disorder will be explored: self/object relations theories, Kohut's theory on narcissism, psychosexual development theories, behavioral manifestation theories, and bodily development theories.4. The role of preoedipal development, as it relates to the formation of the male/female narcissistic personality disorder, will be examined. This section of the paper will propose that, in the narcissistic personality disorder, a pathological arrest occurred during the second or third year of life in response to trauma experienced at that time. The degree and timing of the trauma and the degree of structuralization preceding the trauma all contribute to the rigidity of the narcissistic disorder and the severity of the pathology. 5. The role of oedipal development in the male/female narcissistic personality disorder will be discussed. This discussion will address the intrapsychic configurations which arise during the oedipal period, after narcissistic defenses have solidified during the preoedipal years. While narcissism can be seen as a developmental line, with narcissistic defenses arising at any time during development, this paper will focus primarily on defenses which arise during the separation/individuation phases of development.

Dibujando la arquitectura olvidada: las defensas militares de la guerra de 1936-39 [PPT]

A decade before there was getting up the 'Atlantic Wall', there was executed a system of defenses along the Mediterranean coast in Spain (1936-39). The recovery of the same constructions (both of his graphical documents and of the constructed works that stay in foot) and his putting in value it can help to consolidate an own memory of the 20th century. This work considers to inventory, to measure and to draw the planes of these architectures to fix the memory that is diluted by the erosion of the time. The military pieces place in many borders: are these properly architecture? These are walking between two epochs: the one that perpetuates the epic acts in opposite to the one that shows the disasters in order that they do not forget. They are the most modern ruins of our history. In this process of reconstruction of the memory, there turns out to be crucial the graphical restitution. The drawing is a source of knowledge and demonstrates facts that were constructed.

Dibujando la arquitectura olvidada: las defensas militares de la guerra de 1936-39

Comunicación presentada en el XI Congreso Internacional de Expresión Gráfica Aplicada a la Edificación, APEGA 2012, Valencia, 29 noviemnre-1 diciembre 2012.

Muro Mediterráneo versus movimiento moderno

Una década antes de que fuera construido el Muro Atlántico fue ejecutado un sistema de defensas a lo largo de la costa del Mediterráneo español (1936-39). La recuperación de estas construcciones (de sus documentos gráficos) y su puesta en valor pueden ayudar a consolidar una memoria propia del siglo XX. Este trabajo consiste en inventariar, medir y dibujar los planos de estas arquitecturas a fin de fijar la memoria que se diluye por la erosión del tiempo. Estas defensas militares se sitúan en muchas fronteras: ¿son propiamente arquitectura o piezas industriales? ¿Son arquitectura moderna? Estas transitan entre dos mundos: uno que proyecta arquitecturas ligeras, flexibles y con caducidad frente a otro que construye obras compactas, rígidas y eternas. También se mueven por dos épocas: una que perpetúa las hazañas épicas frente a otras que muestra los desastres. Espacio, tiempo y materia. Son las ruinas de hormigón más modernas de nuestra historia que se encuentran camufladas en la topografía: templos y tumbas a la vez. En esta reconstrucción de la memoria, resulta crucial la restitución gráfica que es donde comienza el conocimiento.

Sequencing and functional analysis of the genome of a nematode egg-parasitic fungus, Pochonia chlamydosporia

Pochonia chlamydosporia is a worldwide-distributed soil fungus with a great capacity to infect and destroy the eggs and kill females of plant-parasitic nematodes. Additionally, it has the ability to colonize endophytically roots of economically-important crop plants, thereby promoting their growth and eliciting plant defenses. This multitrophic behavior makes P. chlamydosporia a potentially useful tool for sustainable agriculture approaches. We sequenced and assembled ∼41 Mb of P. chlamydosporia genomic DNA and predicted 12,122 gene models, of which many were homologous to genes of fungal pathogens of invertebrates and fungal plant pathogens. Predicted genes (65%) were functionally annotated according to Gene Ontology, and 16% of them found to share homology with genes in the Pathogen Host Interactions (PHI) database. The genome of this fungus is highly enriched in genes encoding hydrolytic enzymes, such as proteases, glycoside hydrolases and carbohydrate esterases. We used RNA-Seq technology in order to identify the genes expressed during endophytic behavior of P. chlamydosporia when colonizing barley roots. Functional annotation of these genes showed that hydrolytic enzymes and transporters are expressed during endophytism. This structural and functional analysis of the P. chlamydosporia genome provides a starting point for understanding the molecular mechanisms involved in the multitrophic lifestyle of this fungus. The genomic information provided here should also prove useful for enhancing the capabilities of this fungus as a biocontrol agent of plant-parasitic nematodes and as a plant growth-promoting organism.

Muro Mediterráneo: arquitecturas para la defensa de la Guerra de España (1936-1939)

Una década antes de que fuera construido el Muro Atlántico, fue ejecutado un sistema de defensas a lo largo de la costa del Mediterráneo español (1936-39). La recuperación de estas construcciones (de sus documentos gráficos y de las obras que existen) y su puesta en valor puede ayudar a consolidar una memoria propia del siglo XX. Las piezas militares se sitúan en muchas fronteras: ¿son estas defensas arquitectura o piezas industriales? ¿Son arquitectura moderna? Estas transitan entre dos mundos: uno que proyecta arquitecturas ligeras, flexibles y con caducidad frente a otro que construye obras compactas, rígidas y eternas. Espacio, tiempo y materia. Son las ruinas de hormigón más modernas de nuestra historia camufladas en la topografía: templos y tumbas a la vez.

Muro Mediterráneo: búnkeres y baterías para la defensa del litoral (1936-1939)

The Mediterranean wall, which is a collection of defensive constructions along the coast, was built during the Spanish War (1936-39) to prevent enemy attacks. It´s called this way like the Atlantic Wall, which was built after the Second World War. These group of buildings consist of batteries, bunkers and barracks placed along the coastline, sometimes next to another kind of infrastructure. Its location (typical of a military strategy) and its peculiar morphology are like another ones: the historical watchtowers ones. They were built by the Kingdom of Spain in the same geography four centuries earlier although, in our case, the buildings are updated to the conditions of contemporary wars: camouflage against air raids. A collection of anti-aircraft devices, placed along the coast since the late 1937, were risen following the instructions of the Valencian State to defend both citizens and cities from the aviation´s bombings. The following military settlements, organized from North to South, are part of the most relevant ones of the coast of Alicante: the Denia and Javea ones, the North of Alicante and Southwest of Alicante ones, the Portichol one, the Galvany´s Clot one and, finally, the Cape and Bay of Santa Pola ones. Remains of more than 60 architectural elements, that document the first concrete´s ruins, are still there. This paper tries to document all of them (providing their location, their morphological genealogy and including some drawings of the current state) to contribute to their revaluation and to help to their necessary protection. They are a legacy of architectural heritage which consolidates and increases the memory of our culture.

Letter book : manuscript, 1764-1771

Tryon's official letter book containing copies of letters and documents relating to his work as colonial governor of North Carolina; with references concerning drawing a boundary between North Carolina and South Carolina; talks with the Cherokee Indians; the establishment of a postal service; working with the Propagation of the Gospels in Foreign Parts; establishing the Church of England in North Carolina; strengthening the defenses of the colony; maintaining better communication with Great Britain; and other events.

Dunkerque, France, 1695 (Raster Image)

This layer is a georeferenced raster image of the historic paper map entitled: Plan en profil van Duynkerken : met sijn sterktens en zee-kasteelen, belegert te water, engebombardeert door de Engelse en Hollantse zee-magten, onder de Engelse admiraal Berkly en Hollantse admiraal Alemonde, op den Augustus, 1695, doen maken en uytgeven na de autentijke tekeninge van Monsieur Pamer, Ingenieur van Sijn Kon. Majest. van Groot Brittannien. It was published by Pieter Persoy, op den Dam in 1695. Scale [ca. 1:13,000]. Covers a portion of Dunkerque, France. Map in Dutch. The image inside the map neatline is georeferenced to the surface of the earth and fit to the European Datum 1950, Universal Transverse Mercator (UTM) Zone 31N projected coordinate system. All map collar and inset information is also available as part of the raster image, including any inset maps, profiles, statistical tables, directories, text, illustrations, index maps, legends, or other information associated with the principal map. This map shows features such as roads, drainage, built-up areas and selected buildings, fortifications and defenses, canals, docks, ground cover, and more. Includes also index and panorama view.This layer is part of a selection of digitally scanned and georeferenced historic maps from the Harvard Map Collection. These maps typically portray both natural and manmade features. The selection represents a range of originators, ground condition dates, scales, and map purposes.

Lille, France, ca. 1709 (Raster Image)

This layer is a georeferenced raster image of the historic paper map entitled: Plan de la ville et citadelle de Lille, P. v. Call fecit. It was published by chez Anna Beek ca. 1709. Scale [ca. 1:9,281]. Covers Citadelle de Lille and a portion of Lille, France. Map in French.The image inside the map neatline is georeferenced to the surface of the earth and fit to the European Datum 1950, Universal Transverse Mercator (UTM) Zone 31N projected coordinate system. All map collar and inset information is also available as part of the raster image, including any inset maps, profiles, statistical tables, directories, text, illustrations, index maps, legends, or other information associated with the principal map. This map shows features such as roads, drainage, built-up areas and selected buildings, fortifications, defenses, and lines of fire, ground cover, and more. Relief shown by hachures. Includes also index.This layer is part of a selection of digitally scanned and georeferenced historic maps from the Harvard Map Collection. These maps typically portray both natural and manmade features. The selection represents a range of originators, ground condition dates, scales, and map purposes.

Military Districts, England and Wales, United Kingdom, 1804 (Raster Image)

This layer is a georeferenced raster image of the historic paper map entitled: A complete representation of the coast of England, together with the interior, divided into counties and military districts : also the coast of France and Holland from the Texel to Brest ... to which is annexed eighteen plans of the ports of the enemy, the principal depôts of the flotilla intended for the invasion of England, by John Luffman, Geogr. It was published by J. Luffman in 1804. Scale [ca. 1:2,500,000]. The image inside the map neatline is georeferenced to the surface of the earth and fit to the 'British National Grid' coordinate system. All map collar and inset information is also available as part of the raster image, including any inset maps, profiles, statistical tables, directories, text, illustrations, index maps, legends, or other information associated with the principal map. This map shows features such as drainage, cities and other human settlements, administrative and military district boundaries, shoreline features including distances between selected ports, and more. Includes insets of foreign military installations and index to the military districts. This layer is part of a selection of digitally scanned and georeferenced historic maps from the Harvard Map Collection. These maps typically portray both natural and manmade features. The selection represents a range of originators, ground condition dates, scales, and map purposes.

Battle of Prestonpans, Highlands of Scotland, United Kingdom, 1745 (Raster Image)

This layer is a georeferenced raster image of the historic paper map entitled: An exact plan of His Majesty's great roads through the Highlands of Scotland, And. Rutherfurd delin. ; C. Mosley sculp. It was published in 1745. Scale [ca. 1:300,000].The image inside the map neatline is georeferenced to the surface of the earth and fit to the Europe Lambert Conformal Conic coordinate system. All map collar and inset information is also available as part of the raster image, including any inset maps, profiles, statistical tables, directories, text, illustrations, index maps, legends, or other information associated with the principal map. This map shows features such as drainage, cities and other human settlements, roads, selected buildings, shoreline features, fortification, troop dispositions of Jacobite and the Royal Government's troops for the Battle of Prestonpans, 1745, and more. Relief shown pictorially. Includes inset: Plan of the Battle of Preston fought 21 Sept. 1745. The inset is oriented with south at the top and the entire inset map, including text, is at right angles to the main map.This layer is part of a selection of digitally scanned and georeferenced historic maps from the Harvard Map Collection. These maps typically portray both natural and manmade features. The selection represents a range of originators, ground condition dates, scales, and map purposes.

Sevastopol’’, Ukraine, 1854 (Raster Image)

This layer is a georeferenced raster image of the historic paper map entitled: Plan Sevastopoli͡a s ukrʺplenīi͡ami ot rʺki Belʹbek do Balaklavy : i s oznachenĭemʹ vsʺkh osadnykh raspolozhenīĭ. It was published by Izd. A. Beggrova in 1854. Scale [ca. 1:53,000]. Covers Sevastopol’, Ukraine. Map in Russian. The image inside the map neatline is georeferenced to the surface of the earth and fit to the European Datum 1950, Universal Transverse Mercator (UTM) Zone 36N projected coordinate system. All map collar and inset information is also available as part of the raster image, including any inset maps, profiles, statistical tables, directories, text, illustrations, index maps, legends, or other information associated with the principal map. This map shows features such as drainage, cities and other human settlements, territorial boundaries, shoreline features, roads, built-up areas, selected buildings including defenses and fortification related to he Siege of Sevastopol’, Ukraine, during the Crimean War, 1854-1855, and more. Relief shown by hachures.This layer is part of a selection of digitally scanned and georeferenced historic maps from The Harvard Map Collection as part of the Imaging the Urban Environment project. Maps selected for this project represent major urban areas and cities of the world, at various time periods. These maps typically portray both natural and manmade features at a large scale. The selection represents a range of regions, originators, ground condition dates, scales, and purposes.