Effect of beta-lactamase-catalyzed hydrolysis of cephalosporins on peroxynitrite-mediated nitration of serum albumin and cytochrome c

The hydrolysis of beta-lactam antibiotics by beta-lactamases is one of the major bacterial defense systems. These enzymes generally hydrolyze a variety of antibiotics including the latest generation of cephalosporins, cephamycins and imipenem. In this paper, the effect of cephalosporins-based antibiotics on the peroxynitrite-mediated nitration of protein tyrosine is described. Although some of the antibiotics have weak inhibitory effect on the nitration reactions in the absence of beta-lactamase, they exhibit very strong inhibition in the presence of beta-lactamase. This is due to the elimination of heterocyclic thiol/thione moieties from cephalosporins by beta-lactamase-mediated hydrolysis. After the elimination, the thiols/thiones effectively scavenge peroxynitrite, leading to the inhibition of the nitration reactions.

Antitumor and antioxidant status of Terminalia catappa against Ehrlich ascites carcinoma in Swiss albino mice

Objective: The present study was undertaken to evaluate the antitumor and antioxidant status of ethanol extract of Terminalia catappa leaves against Ehrlich ascites carcinoma (EAC) in Swiss albino mice. Materials and Methods: The leaves powder was extracted with Soxhlet apparatus and subjected to hot continuous percolation using ethanol (95% v/v). Tumor bearing animals was treated with 50 and 200 mg/kg of ethanol extract. EAC induced in mice by intraperitoneal injection of EAC cells 1 x 10(6) cells/mice. The study was assed using life span of EAC-bearing hosts, hematological parameters, volume of solid tumor mass and status of antioxidant enzymes such as lipid peroxidation (LPO), reduced glutathione (GSH), superoxide dismutase (SOD) and catalase (CAT) activities. Total phenolics and flavonoids contents from the leaves extract were also determined. Results: Total phenolics and flavonoids contents from the leaves extract were found 354.02 and 51.67 mg/g extract. Oral administration of ethanol extract of T. catappa (50 and 200 mg/kg) increased the life span (27.82% and 60.59%), increased peritoneal cell count (8.85 +/- 0.20 and 10.37 +/- 0.26) and significantly decreased solid tumor mass (1.16 +/- 0.14 cm(2)) at 200 mg/kg as compared with EAC-tumor bearing mice (P < 0.01). Hematological profile including red blood cell count, white blood cell count, hemoglobin (11.91 +/- 0.47 % g) and protein estimation were found to be nearly normal levels in extract-treated mice compared with tumor bearing control mice. Treatment with T. catappa significantly decreased levels of LPO and GSH, and increased levels of SOD and CAT activity (P < 0.01). Conclusion: T. catappa exhibited antitumor effect by modulating LPO and augmenting antioxidant defense systems in EAC bearing mice. The phenolic and flavonoid components in this extract may be responsible for antitumor activity.

Background paper on the international seafood trade and poverty

(121 p.)

STREAM Journal, Vol. 1, No. 1, pp. 1-12. January-March 2002

CONTENTS: Learning from each other about conflict, by Ronet Santos. E-learning to support knowledge sharing in aquatic resources, by Robert T. Raab and Jonathan Woods. Livelihood strategies, gender and participation in aquaculture: findings from participatory research in northwestern Sri Lanka, by Lindsay J. Pollock and David C. Little. Farming of giant tiger shrimp in northern central Vietnam, by Jesper Clausen. Interacting with stakeholders and policy-makers, by To Phuc Tuong. The role of nutrition communications in meeting the nutritional challenges of the Asia-Pacific region, by Georgina Cairns. (PDF has 16 pages.)

STREAM Journal, Vol. 1, No. 2, pp 1-16. April-June 2002

CONTENTS: Efforts of a farmer in fish seed production for self-employment, by Ras Behari Baraik and Ashish Kumar. Remembering: the missing capacity, by Terrence Clayton. Measuring the process, by Nick Innes-Taylor. Women’s fish farmers group in Nawalparasi, Nepal, by S.K. Pradhan. Periphyton-based aquaculture: a sustainable technology for resource-poor farmers, by M.E. Azim, M.A. Wahab, M.C.J. Verdegem, A.A. van Dam and M.C.M. Beveridge. Unlocking information on the Internet: STREAM media monitoring and issue tracking, by Paul Bulcock (PDF has 16 pages.)

STREAM Journal, Vol. 1, No. 3, pp 1-16. July-September 2002

CONTENTS: From resource user to resource manager: a significant change story, by Ruperto Aleroza as told to Jocel Pangilinan and Ronet Santos. Significant change with Cambodian provincial livelihoods study teams, by Bun Hay Chheng, Tan Someth Bunwhat, Mey Chanthou and Bun Puthy. The Community Fisheries Development Office: one year on, by Matt Fox. CFDO open for business, by Louise Mackeson-Sandbach. Stakeholders and institutional involvement in aquaculture management and development, by M. Krishnan and Pratap S. Birthal. Fish seed production for aquaculture in southeast Cambodia: decentralization, the way to go, by Olivier Delahaye Gamucci, Graham C. Mair and Harvey Demaine.

STREAM Journal, Vol. 1, No. 4, pp 1-16. October-December 2002

CONTENTS: Hon Mun MPA Pilot Project on community-based natural resources management, by Nguyen Thi Hai Yen and Bernard Adrien. An experience with participatory research in Tam Giang Lagoon, Thua Thien-Hue, by Ton That Chat. Experiences and benefits of livelihoods analysis, by Michael Reynaldo, Orlando Arciaga, Fernando Gervacio and Catherine Demesa. Lessons learnt in implementing PRA in livelihoods analysis, by Nguyen Thi Thuy. Lessons learnt from livelihoods analysis and PRA in the Trao Reef Marine Reserve, by Nguyen Viet Vinh. Using the findings from a participatory poverty assessment in Tra Vinh Province, by Le Quang Binh.

STREAM Journal, Vol. 2, No. 1, pp 1-18. January-March 2003

CONTENTS: Learning insights from the Fisheries Resource Management Project, by Tee-Jay A San Diego. An orientation on the SIAD approach and participatory local development planning, by Elizabeth M. Gonzales. Group-building, production success and the struggle to prevent capture of the resource, by B.K. Sahay, K.P. Singh and S.N. Pandeya. Urban agriculture, water reuse and local economies: case study of coastal riverine Settlements of Ondo State, Nigeria, by Yemi Akegbejo-Samsons. Livelihoods analysis: actual experience from using PRA, by Pham Minh Tam and Trinh Quang Tu. A sustainable livelihoods approach to fisheries development for poverty alleviation in southeastern Vietnam, by Nguyen Van Tu and Nguyen Minh Duc

STREAM Journal, Vol. 2, No. 2, pp 1-19. April-June 2003

CONTENTS: Livelihoods and languages: a SPARK-STREAM learning and communications process, by Kath Copley and William Savage. Towards broader contextual understandings of livelihoods, by Elizabeth M. Gonzales, Nguyen Song Ha, Rubu Mukherjee, Nilkanth Pokhrel and Sem Viryak. Using tools to build shared understandings, using a sustainable livelihoods framework to learn, by Nuchjaree Langkulsane. Learning about rattan as a livelihood, by Mariel de Jesus and Christine Bantug. Meanings of “community-managed area,” by Arif Aliadi. Lessons learnt about processes for learning and communicating, by Graham Haylor and Ronet Santos.

STREAM Journal, Vol. 2, No. 3, pp 1-18. July-September 2003

CONTENTS: Livelihood Improvements through fisheries in the Pode community in Pokhara, Nepal, by Tek Bahadur Gurung and Jay Dev Bista. Women’s participation in coastal resources management and livelihoods in Khanh Hoa Province, Vietnam, by Nguyen Thu Hue, Than Thi Hien, Pham Thi Phuong Hoa, Nguyen Viet Vinh and Dao Viet Long. Supporting people’s efforts and interactions in coastal resources management in Indonesia, by Tabitha Yulita. Planning for a community fisheries M&E system, by Heather Airlie and Haiko Meelis. Identifying needs and recommendations for efficient stakeholder communications through an information access survey, by Elizabeth M. Gonzales, Malene Felsing and Erwin L Pador. IEC seminar-workshop in support of fisheries ordinance implementation in Roxas City, Philippines, by Belinda M. Garrido and Elizabeth M. Gonzales.

STREAM Journal, Vol. 2, No. 4, pp 1-18. October-December 2003

CONTENTS: An experience with group formation in Jabarrah, West Bengal, by Amar Prasad, Virendra Singh, Binay Sahay and Gautum Dutta. Aquaculture self-help groups in rural West Bengal, by Satyendra Tripathi, Graham Haylor and William Savage in consultation with Jagdish Gangwar, Virendra Singh, Gautam Dutta and Prabhat Pathak. Lessons learned for Fulwar Toli from an exposure visit to Jabarrah Bhim Nayak and Rubu Mukherjee. Livelihood intervention by Fish Farmers Development Agency: a success story from Mayurbhanj, Orissa, by G.B. Parida. Understanding of assets-based livelihoods through participatory rural appraisal to eliminate hunger, by Binay Sahay. Poverty ranking tools in PRA: experiences of EIRFP in addressing vulnerability, by Binay Sahay.

STREAM Journal, Vol. 3, No. 1, pp 1-18. January-March 2004

CONTENTS: Creating understanding and ownership of collaborative research results through ‘learning by doing,’ by Robert Arthur and Caroline Garaway. Fish culture, farming, markets and promotion: an integrated, sustainable approach to aquaculture and rural development, by Pen Rotha and Brendan Boucher. Fisheries policy reform impact assessment in Cambodia: understanding policy and poor people, by Philip Townsley and Sem Viryak. “Shrimp Hero” Phan The Phuong, by Ngo Minh Khoi. Coral farming in Vietnam, by Nguyen Viet Vinh. The global fisheries market: can rural poor people benefit? Issues raised by STREAM Media Monitoring Reports, by Paul Bulcock.

STREAM Journal, Vol. 3, No. 2, pp 1-17. April-June 2004

CONTENTS: BFAR moves on in Region 6, by Janice N. Tronco. Livelihoods initiatives in Sapian Bay, by Tee-Jay A. San Diego. Improving access to information through Barangay Learning Resource Centers, by Elizabeth M. Gonzales. The Philippines Fisheries Information System, by Agnes C. Solis. The contributions of planning activities in the participatory process, by Rommel P. Guarin. Inter-LGU alliance building: a key to sustaining the Integrated Fisheries and Aquatic Resource Management Council (IFARMC), by Josephine P. Savaris.

STREAM Journal, Vol. 3, No. 3, pp 1-17. July-September 2004

CONTENTS: What do we need to learn about to understand how people live, by Kath Copley and William Savage. Taking steps to making livelihoods work, by Erwin L. Pador. Reflections on the India-Nepal Workshop, by Rubu Mukherjee and Nilkanth Pokhrel. Reflections on the role of livelihoods approaches and analysis in Lao PDR, by Phanthavong Vongsamphanh and Graham Haylor. After the Workshop on Livelihoods Approaches and Analysis, by Khin Maung Soe, Aye Aye Zaw, Nilar Kyawe and Myant Thar Htun. The relevance of livelihoods approaches in Yunnan, China, by Susan Li and William Savage