999 resultados para Custo da transação


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A strain of the flamentous fungus Aspergillus niger was isolated and shown to possess extracellular xylanolytic activity. These enzymes have biotechnological potential and can be employed in various industries. This fungus produced its highest xylanase activity in a medium made up of 0.1% CaCO3, 0.5% NaCl, 0.1% NH4Cl, 0.5% corn steep liquor and 1% carbon source, at pH 8.0. A low-cost hemicellulose residue (powdered corncob) proved to be an excellent inducer of the A. niger xylanolytic complex. Filtration of the crude culture medium with suspended kaolin was ideal for to clarify the extract and led to partial purifcation of the xylanolytic activity. The apparent molecular mass of the xylanase was about 32.3 kDa. Maximum enzyme activity occurred at pH 5.0 and 55-60oC. Apparent Km was 10.41 ± 0.282 mg/mL and Vmax was 3.32 ± 0.053 U/mg protein, with birchwood xylan as the substrate. Activation energy was 4.55 kcal/mol and half-life of the crude enzyme at 60oC was 30 minutes. Addition of 2% glucose to the culture medium supplemented with xylan repressed xylanase production, but in the presence of xylose the enzyme production was not affected.

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As the psychrometric method is considered standard for the measurement of relative humidity, many studies have used aspirated thermocouple psychrometers connected to microloggers but, however, they do not detail how the aspiration of the air stream is done. However, one of the major difficulties of aspirated thermocouple psychrometers is to program and to connect it in micrologger, because specific programming and connection are requires, and still some skills in getting accurate measurements. This work aimed to provide the programming and the connection of the aspirated thermocouple psychrometer type T for the CR10X microloggers and CR23X of Campbell Scientific, evaluating the quality of measurements of temperature and air relative humidity in relation the a sensor Vaisala HMP50. The non-continuous measurements were made in the period 2006/09/04 to 2007/07/11 at Jaboticabal, SP. The air temperature measurements were similar between the Vaisala sensors and aspirated thermocouple psychrometer, but the relative humidity measurements were significantly different. The measurements obtained by the aspirated thermocouple psychrometer connected to CR10X micrologger were the most accurate. Using the programming and connection of a micro-fan to suck in the dry and moist bulbs of aspirated thermocouple psychrometer in microloggers models CR10X and CR23X, it is possible to obtain measurements of air temperature with good precision and accuracy, and measurements of air relative humidity with good precision, but accuracy is not always attainable due to the difficulties inherent to the physical processes that occur in the wet bulb, the skill in the use and maintenance of this equipment and the reservoir protection against radiative effects.

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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

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Pós-graduação em Biometria - IBB

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Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

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Pós-graduação em Agronomia (Energia na Agricultura) - FCA

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Pós-graduação em Agronomia (Energia na Agricultura) - FCA

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Pós-graduação em Agronomia (Energia na Agricultura) - FCA