Executive Order Number Fifty Five, June 22, 1995

Appoints the Des Moines resident-in-charge for the U.S. Drug Enforcement Adminstration as a nonvoting ex-officio member of the Iowa Law Enforcement Academy Council.

Genometrics as an essential tool for the assembly of whole genome sequences: the example of the chromosome of Bifidobacterium longum NCC2705.

BACKGROUND: Analysis of the first reported complete genome sequence of Bifidobacterium longum NCC2705, an actinobacterium colonizing the gastrointestinal tract, uncovered its proteomic relatedness to Streptomyces coelicolor and Mycobacterium tuberculosis. However, a rapid scrutiny by genometric methods revealed a genome organization totally different from all so far sequenced high-GC Gram-positive chromosomes. RESULTS: Generally, the cumulative GC- and ORF orientation skew curves of prokaryotic genomes consist of two linear segments of opposite slope: the minimum and the maximum of the curves correspond to the origin and the terminus of chromosome replication, respectively. However, analyses of the B. longum NCC2705 chromosome yielded six, instead of two, linear segments, while its dnaA locus, usually associated with the origin of replication, was not located at the minimum of the curves. Furthermore, the coorientation of gene transcription with replication was very low. Comparison with closely related actinobacteria strongly suggested that the chromosome of B. longum was misassembled, and the identification of two pairs of relatively long homologous DNA sequences offers the possibility for an alternative genome assembly proposed here below. By genometric criteria, this configuration displays all of the characters common to bacteria, in particular to related high-GC Gram-positives. In addition, it is compatible with the partially sequenced genome of DJO10A B. longum strain. Recently, a corrected sequence of B. longum NCC2705, with a configuration similar to the one proposed here below, has been deposited in GenBank, confirming our predictions. CONCLUSION: Genometric analyses, in conjunction with standard bioinformatic tools and knowledge of bacterial chromosome architecture, represent fast and straightforward methods for the evaluation of chromosome assembly.

Fulgentius, Mythologie, phisica expositio super XII libros eneidorum (16), expositio sermonum ad Calcidium (20 v°). - Pauli et Senece epistole (22 v°). - Xenia M. Valerii Martialis (23). - Honorius, philosophia mundi (26 v°). - Prologus quorumdam juridicorum tractatuum (54 v°). - Ex Ausonio (55). -

Cordeliers.

Johannes de Monsterolio, Libellus adversus Anglos (1v-22), Epistola 202 (22v-26v), Per brevis epilogus gestorum Karoli Magni (26v-28v).

Ms. autographe de Jean de Montreuil. D'après G. Ouy, Jean de Montreuil a copié l'année précédant sa mort (1418) la lettre 202 sur des feuillets inemployés à la suite d'une copie originale de son traité sur les prétentions des rois d'Angleterre à la couronne de France, composé en 1415.

CONFERENCE AVEC M. CLAUDE Ministre de Charenton, Sur la Matiere de l'Eglise. Par Messire Jacques Benigne Bossuet, Evesque de Meaux, Conseiller du Roy en ses Conseils, cy-devant Précepteur de Monseigneur le Dauphin, premier Aumosnier de Madame la Dauphine. A Paris, Chez Sebastien Mabre-Cramoisy, Imprimeur du Roy, ruë Saint Jacques, aux Cicognes. M.DC.LXXXII [1682]. Avec Privilege du Roy. In-12 de 22 f., 504 p. et 1 f. pour l'Extrait du Privilége, mar. r., fil., dos orné, tr. dor. (Anc. rel.)

Bossuet (Jacques-Bénigne). Conference avec M. Claude (1682)

Författaren Christer Kihlman 22.4.1972 Finnå

Kirje

ABD e-News - September 22, 2006

Weekly Newsletter

Motor Vehicle Accident Fatalities for the Week Ending, September 22, 2006

Record of the Fatalities for Motor Vehicle Accidents in Iowa per week.

EPI Update, September 22, 2006

Weekly newsletter for Center For Acute Disease Epidemiology of Iowa Department of Public Health.

Småarbetet med socialdemokraterna inleddes (tal den 22.1.1951 i Kuopio)

Puhe

Sentence du Chastelet de Paris qui bannit à perpétuité hors du royaume le nommé Duval, vicaire de la paroisse Saint Leu Saint Gilles, pour avoir fait le refus schismatique d'administrer le sacrement de l'extrême-onction, quoique sommé, au sieur Leguay, prêtre,... Du jeudi 22 juillet 1762

[Acte. 1762-07-22]