Directed evolution of polymerase function by compartmentalized self-replication

We describe compartmentalized self-replication (CSR), a strategy for the directed evolution of enzymes, especially polymerases. CSR is based on a simple feedback loop consisting of a polymerase that replicates only its own encoding gene. Compartmentalization serves to isolate individual self-replication reactions from each other. In such a system, adaptive gains directly (and proportionally) translate into genetic amplification of the encoding gene. CSR has applications in the evolution of polymerases with novel and useful properties. By using three cycles of CSR, we obtained variants of Taq DNA polymerase with 11-fold higher thermostability than the wild-type enzyme or with a >130-fold increased resistance to the potent inhibitor heparin. Insertion of an extra stage into the CSR cycle before the polymerase reaction allows its application to enzymes other than polymerases. We show that nucleoside diphosphate kinase and Taq polymerase can form such a cooperative CSR cycle based on reciprocal catalysis, whereby nucleoside diphosphate kinase produces the substrates required for the replication of its own gene. We also find that in CSR the polymerase genes themselves evolve toward more efficient replication. Thus, polymerase genes and their encoded polypeptides cooperate to maximize postselection copy number. CSR should prove useful for the directed evolution of enzymes, particularly DNA or RNA polymerases, as well as for the design and study of in vitro self-replicating systems mimicking prebiotic evolution and viral replication.

Corporate social responsibility: a European perspective. Jean Monnet/Robert Schuman Paper Series Vol. 13 No. 6, June 2013

From the Introduction. CSR grows at different rhythms. CSR varies from continent to continent, country from country, sector from sector and corporation from corporation. The Responsible Competitive Index (RCI) from the UK NGO Accountability and the Brazilian Business School, Fundaçao Dom Cabral, looks at how countries are performing in their efforts to promote responsible business practices and issues periodical indexes about such performances. The RCI’s index for 2007 analysed 108 countries (96% of global GDP). The analysis showed that more advanced economies do better in this area. The top 20 countries, by the ranking order of best performance, were the following: 1 Sweden, 2 Denmark, 3 Finland, 4 Iceland, 5 UK, 6 Norway, 7 New Zealand, 8 Ireland, 9 Australia, 10 Canada, 11 Germany, 12, Netherlands, 13 Switzerland, 14 Belgium, 15 Singapore, 16 Austria, 17 France, 18 USA, 19 Japan, and 20 Hong Kong, etc. However, it is important to bear in mind that advanced economies have often moved their more dirty industries to other parts of the world where there are less stringent environmental and social standards. As a result, other countries may be polluting on their behalf, and the indexes do not factor those in.2

Misurazione della Sostenibilità in Azienda. Approccio Quantitativo all'Analisi della Letteratura attraverso Bibliografic Coupling.

L'elaborato si impegna ad esporre i risultati derivanti da una revisione estensiva della letteratura riguardante le pratiche di misurazione della sostenibilità in azienda. Si è scelto un approccio al problema di impostazione bibliometrico, consistente in analisi statistiche di dati bibliografici ricavabili osservando le relazioni tra documenti. Si sono utilizzati strumenti di clustering per categorizzare la letteratura ed individuare le aree di maggior interesse potenziali per aziende che desiderino investire con successo nella sostenibilità. Come risultato, si è ottenuta una chiara identificazione delle principali tematiche che devono essere tenute in considerazione nell’affrontare valutazioni relative alla misurazione e valorizzazione dei concetti di sostenibilità e impatto ambientale in ambito aziendale. Il presente lavoro si presenta inoltre come guida metodologica per un approccio quantitativo alla revisione della letteratura, adattabile ed applicabile in maniera iterativa per un continuo monitoraggio del tema o per indagini in altri ambiti. Questa tesi si pone dunque come base per ricerche e analisi successive finalizzate alla comprensione della letteratura.

Radon 222 excess measured on water bottle samples during HEINCKE cruise HE153

In the years 2000 and 2001 we measured methane concentrations exceeding up to two orders of magnitude the equilibrium with the atmosphere in the water column on the SW-Spitsbergen continental shelf. This methane anomaly extended from its centre on the shelf westwards over the upper slope and eastwards well into the inner basins of the two southernmost Spitsbergen fjords, the Hornsundfjord and the van Mijenfjord. Methane concentrations and stable carbon isotopic ratios varied between 2 and 240 nM, and between -53 per mill and -20 per mill VPDB, respectively. Methane in high concentrations was depleted in 13C whereas in low concentrations d13CCH4 values were highly variable. On the continental shelf we found that methane discharged from seeps on top of sandy and gravelly banks is isotopically heavier than methane escaping from troughs filled with silty and clayey sediments. These distinct isotopic signatures suggest that methane is gently released from several inter-granular seepages or micro-seepages widely spread over the shelf. A potential migration path for thermogenic or hydrate methane may be the Hornsund Fracture Zone, a south-north running reactivated fault system created by stretching of the continental crust. After discharge into the water column, local water currents fed by Atlantic water, coastal water, and freshwater outflows from the fjords further determine pathways and fate of the methane. We used d18Owater and 222Rn data to trace origin and advection of the local water masses and water mixing processes. Methane spreads predominantly along pycnoclines and by vertical mixing. During transport methane is influenced simultaneously by oxidation and dilution, as well as loss into the atmosphere. Together these processes cause the spatial variability of the anomaly and heterogeneity in d13CCH4 in this polar shelf environment.

Short-term evaluation and research issues for the Job Training Partnership Act of 1982 : impact of different forms of private sector participation on client characteristics and program outcomes : final report /

"December, 1983."

Tillit genom hållbarhet : En kvalitativ studie av hur Axfood skapar tillit genom kommunikationen av deras hållbarhetsarbete under 2015.

SYFTE: Uppsatsens syfte är att kartlägga på vilket sätt Axfood kommunicerar sitt hållbarhetsarbete, i avseende att se hur Axfood upprätthåller tillit genom sin kommunikation. Tillit är en viktig komponent i företagsverksamheten och därför vill jag belysa hur olika stilistiska kompositioner både kan främja och hämma tilliten till ett företag. Studien fokuseras till Axfoods avsändarperspektiv med avsikt att strukturera upp och beskriva hur företaget kommunicerar sitt arbete med hållbarhet. TEORETISK RAM: Den teoretiska ramen bygger på hur tillit skapas genom kommunikation och utgår ifrån teorier om Corporate Social Responsibility, samt det retoriska begreppet ethos. METOD: Övergripande tematisering av materialet, samt en djupgående stilistisk analys av de delar i materialet som kommunicerar Axfoods hållbarhetsarbete. SLUTSATSER: Av Axfoods kommunikation är det hållbarhetsarbetet angående miljö som ges störst utrymme. Vidare går det att utläsa vissa stilistiska skillnader beroende på i vilken kommunikationskanal materialet återfinns, samt vilket hållbarhetsarbete som kommuniceras. Det här påverkar de olika tillvägagångssätten att skapa tillit.

Ekologisk skuldbeläggning? : En kvantitativ och kvalitativ receptionsstudie om Coops film Ekoeffekten

”Ekologisk skuldbeläggning?” är en receptionsstudie författad av Erica Rohlin och Josefin Dahlqvist vid Stockholms Universitet på institutionen för mediestudier vårterminen 2016. Uppsatsen ämnade undersöka hur ett urval av en yngre generation (18-30 år) i Stockholm förhåller sig till Coops film Ekoeffekten, som utformats för att få människor att köpa mer ekologisk mat. Kritik har riktats mot Coops film Ekoeffekten och det står som grund för att utföra en kritisk granskning i form av en receptionsstudie. Det teoretiska ramverket utgår ifrån propaganda, hälsokommunikation, kampanjer och CSR. Teorierna är sedermera sammankopplade med resultat från en kvantitativ surveyundersökning samt två kvalitativa fokusgruppsintervjuer. Resultatet visade att ämnet skuldbeläggning var något som framgick i fokusgruppsintervjuerna, vilket tyder på att detta var en aspekt respondenterna uppfattade mer än andra. Deltagarna ansåg att Coop genom Ekoeffekten placerar skuld på familjen Palmberg och sedermera på konsumenter som inte handlar ekologisk mat. Vidare visade en korrelationstabell på ett samband som kan styrka att Coop gör närmande mot skrämselpropaganda, då en del deltagare blev oroliga för sin hälsa baserat på Coops studie, gjord av IVL, som saknar vetenskaplig signifikans. Respondenterna från surveyundersökningen samt fokusgruppsintervjun visade att de uppfattar Ekoeffekten som en kombinerad informations- och reklamkampanj. Fokusgruppsintervjun visade respondenternas provocerande attityd gentemot detta, vilket inte gick att avläsa från surveyundersökningen. Detta eftersom Ekoeffekten troligtvis inte är provocerande vid en första anblick, utan det är vid vidare diskussioner som åsikter och attityder kom till tals. 

Greetings from the cybercaliphate: some notes on homeland insecurity

One of the paradoxical effects of the 7 July bombings in London was to expose the ambivalence in the British government's attempt to wage war on terror by forcefully prosecuting war against those who resort to jihad abroad, actively participating in coalitions of the wining whether in Afghanistan or Iraq, while affording some of Islamism's key ideologists and strategists a high degree of latitude in the United Kingdom itself. This indicates a number of contradictions in official policy that simultaneously recognizes the globalized threat from violent Islamic militancy while, under the rubric of multiculturalism, tolerating those very strains of Islamist radicalism, some of which draw upon the interdependent and transnational character of conflict, to render the UK vulnerable to those very same violent forces. Consequently, the British authorities displayed a studied indifference towards this developing transnational phenomenon both during the 1990s and in some respects even after the London bombings. To explore the curious character of the government's response to the Islamist threat requires the examination of the emergence of this radical ideological understanding and what it entails as a reaction to modernization and secularism in both thought and practice. The analysis explores how government policies often facilitated the non-negotiable identity politics of those promoting a pure, authentic and regenerated Islamic order both in the UK and abroad. This reflected a profound misunderstanding of the growing source and appeal of radical Islam that can be interpreted as a consequence of the slow-motion collision between modernity in its recent globalized form and an Islamic social character, which renders standard western modernization theory, and indeed, the notion of a 'social science' itself, deeply questionable.

Corporate social responsibility reporting in developing countries:the case of Bangladesh

Historically, the study of corporate social responsibility (CSR) reporting has largely been within the context of Western economies. However, in the wake of highly publicized incidents such as Bhopal and the struggle of the Ogoni people in Nigeria, many large corporations now claim to be taking steps to improve their environmental and social performance within developing countries. Using the lens of stakeholder theory, this book examines whether the current practice of CSR reporting in developing countries is motivated by a desire to discharge accountability to all relevant stakeholders or whether it is being driven by the imperative of advancing corporate economic interests. While concepts like CSR reporting have become more fashionable, they vary widely in different national contexts; this book therefore clarifies the types and roles of CSR reporting and the underlying corporate motivations. The author considers the current CSR reporting practices in a number of developing countries, with particular attention given to illuminating a case study of Bangladesh.

A study of corporate social disclosures in Bangladesh

Most of the corporate social reporting (CSR) studies conducted so far have been in the context of developed countries. Very few studies are available on the CSR practices in developing countries. Given the different socio-economic context of developing countries it is argued that it is important to learn about the CSR practices in those countries. This study reports the results of a survey of CSR practices in Bangladesh. The main contribution of this paper is that in addition to measuring the extent and volume of disclosures by using content analysis, it explores the socio-political and economic context in which these disclosures take place.

The views of corporate managers on the current state of, and future prospects for, social reporting in Bangladesh: an engagement based study

Purpose – This paper seeks to respond to recent calls for more engagement-based studies of corporate social reporting (CSR) practice by examining the views of corporate managers on the current state of, and future prospects for, social reporting in Bangladesh. Design/methodology/approach – The paper uses a series of interviews with senior managers from 23 Bangladeshi companies representing the multinational, domestic private and public sectors. Findings – Key findings are that the main motivation behind current reporting practice lies in a desire on the part of corporate management to manage powerful stakeholder groups, whilst perceived pressure from external forces, notably parent companies' instructions and demands from international buyers, is driving the process forward. In the latter context it appears that adoption of international social accounting standards and codes is likely to become more prevalent in the future. Reservations are expressed as to whether such a passive compliance strategy is likely to achieve much in the way of real changes in corporate behaviour, particularly when Western developed standards and codes are imposed without consideration of local cultural, economic and social factors. Indeed, such imposition could be regarded as little more than an example of the erection of non-tariff trade barriers rather than representing any meaningful move towards empowering indigenous stakeholder groups. Originality/value – The paper contributes to the literature on CSR in developing countries where there is a distinct lack of engagement-based published studies.