Grants and loans for municipal water supply and watewater [i.e. wastewater] treatment systems : water conservation provisions : final report of Task Force 11 /

Mode of access: Internet.

Joint development and value capture in Los Angeles : local policy formulation /

"DOT-I-83-23."

Entrepreneurship in Argentine culture; Torcuato Di Tella and S.I.A.M.,

Includes bibliography.

Personal recollections and observations of General Nelson A. Miles, embracing a brief view of the Civil War; or, From New England to the Golden gate, and the story of his Indian campaigns, with comments on the exploration, development and progress of our great western empire;

A campaign against Apaches [1885-86] (Captain Maus' narrative): p. 450-471.

Port development and related maritime matters [microform] : hearings before the Committee on Merchant Marine and Fisheries, House of Representatives, Ninety-sixth Congress, second session, on effort to review and maintain oversight of port development and related maritime matters and to receive comments on the omnibus maritime bill, April 7, 9, and 10, 1980.

"Serial no. 96-51."

National Materials and Minerals Policy, Research, and Development Act of 1980 [microform] : hearing before the Committee on Science and Technology, U.S. House of Representatives, Ninety-seventh Congress, first session, July 28, 1981.

"No. 44."

Proceedings of the Child conference for research and welfare.

1st conference held at Clark university in connection with the celebration of its twentieth anniversary July 6-10, 1909; 2d. held at Clark university, June 28-July 2, 1910.

Adapted physical education : related legislation, IEP development and programmatic considerations for Illinois /

Funded in part by the Area Learning Resource Center and Public Law 94-142.

School sites: selection, development, and utilization.

Bibliography : p. 81-84.

A study of state occupational information development and utilization efforts /

Mode of access: Internet.

Economic Survey of Latin America and the Caribbean 2016: The 2030 Agenda for Sustainable Development and the challenges of financing for development

This, the sixty-eighth edition of the Economic Survey of Latin America and the Caribbean, which corresponds to the year 2016, consists of three parts. Part I outlines the region’s economic performance in 2015 and analyses trends in the first half of 2016, as well as the outlook for the rest of the year. It examines the external and internal factors influencing the region’s economic performance and highlights some of the macroeconomic policy challenges that have arisen in an external context of weak growth and high levels of uncertainty. Part II analyses the challenges that the countries of Latin America and the Caribbean face at the domestic and international levels in mobilizing financing for development. On the domestic front, slower growth and tighter fiscal restrictions pose significant challenges for the mobilization of resources. Externally, the classification of many of the region’s countries in the middle-income category limits their access to concessional external financing or international support. Part III of this publication may be accessed on the web page of the Economic Commission for Latin America and the Caribbean (www.eclac.org). It contains the notes relating to the economic performance of the countries of Latin America and the Caribbean in 2015 and the first half of 2016, together with their respective statistical annexes. The cut-off date for updating the statistical information in this publication was 30 June 2016.

The Discovery, Development, and Mechanistic Investigations of Transition Metal-Catalyzed Organic Reactions

Thesis (Ph.D.)--University of Washington, 2016-06

Elucidating the cis-regulatory landscape of retinal development and regeneration

Thesis (Ph.D.)--University of Washington, 2016-04

Development and evaluation of an automated annotation pipeline and cDNA annotation system

Manual curation has long been held to be the gold standard for functional annotation of DNA sequence. Our experience with the annotation of more than 20,000 full-length cDNA sequences revealed problems with this approach, including inaccurate and inconsistent assignment of gene names, as well as many good assignments that were difficult to reproduce using only computational methods. For the FANTOM2 annotation of more than 60,000 cDNA clones, we developed a number of methods and tools to circumvent some of these problems, including an automated annotation pipeline that provides high-quality preliminary annotation for each sequence by introducing an uninformative filter that eliminates uninformative annotations, controlled vocabularies to accurately reflect both the functional assignments and the evidence supporting them, and a highly refined, Web-based manual annotation tool that allows users to view a wide array of sequence analyses and to assign gene names and putative functions using a consistent nomenclature. The ultimate utility of our approach is reflected in the low rate of reassignment of automated assignments by manual curation. Based on these results, we propose a new standard for large-scale annotation, in which the initial automated annotations are manually investigated and then computational methods are iteratively modified and improved based on the results of manual curation.

Development and transplantation of a mineralized matrix formed by osteoblasts in vitro for bone regeneration

The use of extracellular matrix materials as scaffolds for the repair and regeneration of tissues is receiving increased attention. The current study was undertaken to test whether extracellular matrix formed by osteoblasts in vitro could be used as a scaffold for osteoblast transplantation and induce new bone formation in critical size osseous defects in vivo. Human osteoblasts derived from alveolar bone were cultured in six-well plates until confluent and then in mineralization media for a further period of 3 weeks to form an osteoblast-mineralized matrix complex. Histologically, at this time point a tissue structure with a connective tissue-like morphology was formed. Type I collagen was the major extracellular component present and appeared to determine the matrix macrostructure. Other bone-related proteins such as alkaline phosphatase (ALP), bone morphogenetic protein (BMP)-2 and -4, bone sialoprotein (BSP), osteopontin (OPN), and osteocalcin (OCN) also accumulated in the matrix. The osteoblasts embedded in this matrix expressed mRNAs for these bone-related proteins very strongly. Nodules of calcification were detected in the matrix and there was a correlation between calcification and the distribution of BSP and OPN. When this matrix was transplanted into a critical size bone defect in skulls of inummodeficient mice (SCID), new bone formation occurred. Furthermore, the cells inside the matrix survived and proliferated in the recipient sites, and were traceable by the human-specific Alu gene sequence using in situ hybridization. It was found that bone-forming cells differentiated from both transplanted human osteoblasts and activated endogenous mesenchymal cells. This study indicates that a mineralized matrix, formed by human osteoblasts in vitro, can be used as a scaffold for osteoblast transplantation, which subsequently can induce new bone formation.