Molecular cloning and characterization of cDNA encoding the alpha subunit of the rat protein synthesis initiation factor eIF-2B.

Eukaryotic initiation factor 2B (eIF-2B) is an essential component of the pathway of peptide-chain initiation in mammalian cells, yet little is known about its molecular structure and regulation. To investigate the structure, regulation, and interactions of the individual subunits of eIF-2B, we have begun to clone, characterize, and express the corresponding cDNAs. We report here the cloning and characterization of a 1510-bp cDNA encoding the alpha subunit of eIF-2B from a rat brain cDNA library. The cDNA contains an open reading frame of 918 bp encoding a polypeptide of 305 aa with a predicted molecular mass of 33.7 kDa. This cDNA recognizes a single RNA species approximately 1.6 kb in length on Northern blots of RNA from rat liver. The predicted amino acid sequence contains regions identical to the sequences of peptides derived from bovine liver eIF-2B alpha subunit. Expression of this cDNA in vitro yields a peptide which comigrates with natural eIF-2B alpha in SDS/polyacrylamide gels. The predicted amino acid sequence exhibits 42% identity to that deduced for the Saccharomyces cerevisiae GCN3 protein, the smallest subunit of yeast eIF-2B. In addition, expression of the rat cDNA in yeast functionally complements a gcn3 deletion for the inability to induce histidine biosynthetic genes under the control of GCN4. These results strongly support the hypothesis that mammalian eIF-2 alpha and GCN3 are homologues. Southern blots indicate that the eIF-2B alpha cDNA also recognizes genomic DNA fragments from several other species, suggesting significant homology between the rat eIF-2B alpha gene and that from other species.

The impact of model detail on power grid resilience measures

Peer reviewed

Post Route Map of New York, 1871 (Image 1 of 2) (Raster Image)

This layer is a georeferenced raster image of the historic paper map entitled: Post route map of the state of New York : and parts of Vermont, Massachusetts, Connecticut, New Jersey and Pennsylvania, showing also the adjacent portions of the dominion of Canada, designed and constructed under the orders of Postmaster General, Alex. W. Randall, and second asst. Postmaster General, Geo. Wm. McLellan, by W.L. Nicholson, topographer of P.O. Dept. It was published by U.S. Post Office Dept. in 1868. Ink notation: "The service on this diagram brought up to date of Jan. 1st, 1871." Scale [1:380,160]. This layer is image 1 of 2 total images of the two sheet source map. The image inside the map neatline is georeferenced to the surface of the earth and fit to the Universal Transverse Mercator (UTM) Zone 18N NAD83 projection. All map collar and inset information is also available as part of the raster image, including any inset maps, profiles, statistical tables, directories, text, illustrations, index maps, legends, or other information associated with the principal map. This map shows features such as roads, railroads, postal routes (with delivery schedule frequency and distances), drainage, cities and towns, counties, and more. Includes insets: Postal service of Long Island with the principal mail connections of the city of New York. Scale [1:380,160] -- [Parts of the provinces of Québec and Ontario bordering New York and Vermont]. Includes tables of distances and statistics. This layer is part of a selection of digitally scanned and georeferenced historic maps from The Harvard Map Collection as part of the Imaging the Urban Environment project. Maps selected for this project represent major urban areas and cities of the world, at various time periods. These maps typically portray both natural and manmade features at a large scale. The selection represents a range of regions, originators, ground condition dates, scales, and purposes.

Ahlâk-ı alâ'î : manuscript, undated

A work on morals, completed on 25 Safer 973 AH [21 Sept. 1565 AD], while the author was serving as judge in Damascus. First section is on personnal morals, second on family morals and third on political morals and art of governance.

Aprendizagem cooperativa na infância

Relatório de estágio apresentado para a obtenção do grau de mestre na especialidade de Educação pré-escolar

Expositio biblie in Mammotrectum

Sign.: A-C8, D4, A-Z8, [et]8, [cum]8, 2a-2e8, 2f4

De Roma prisca et nova varii auctores prout in sequenti pagella cernere est.

Contains Francesco Albertini's Opusculum de mirabilibus novae et veteris urbis Romae, leaves I-LXXX, which includes a reference to Vespucci, leaf LXXX.

Little journeys to the homes of eminent artists /

Title page and initials designed by Samuel Warner.

Liber fratrum cruciferorum Leodiensium : [1617].

Comprises 54 organ compositions, chiefly by anonymous composers, but including a few works by A. Gabrielli, P. Philipps, J. Swelinck, C. Merlu, and W. Brwon, transcribed from ms. 888 of University Library. Liège.

Hispaniae schola musica sacra. : Opera varia (saecul. XV, XVI, XVII et XVIII) /

The prefaces, biographical introductions, analytical notes and bibliographies are in Spanish and French.

The mirrour of martyrs : the first and second part ; in a short view, liuely expressing the force of their faith, the feruency of their loue, the wisdome of their sayings, the patience of their suffrings, &c. With their prayers and preparation for their last farewell ; Whereunto are added two godly letters written by Mr. Bradford, full of sweet consolation for all such as are afflicted in conscience.

STC 5850.

Spanish explorers in the southern United States, 1528-1543 : The narrative of Alvar Nuñez Cabeça de Vaca,

Mode of access: Internet.