Intracranial Volume Estimation and Graph Theoretical Analysis of Brain Functional Connectivity Networks

Understanding pathways of neurological disorders requires extensive research on both functional and structural characteristics of the brain. This dissertation introduced two interrelated research endeavors, describing (1) a novel integrated approach for constructing functional connectivity networks (FCNs) of brain using non-invasive scalp EEG recordings; and (2) a decision aid for estimating intracranial volume (ICV). The approach in (1) was developed to study the alterations of networks in patients with pediatric epilepsy. Results demonstrated the existence of statistically significant (p

Avaliação funcional e estrutural de um novo peptídeo antimicrobiano do escorpião Tityus stigmurus

In Brazil, there is a high incidence of venomous animals. Among them, scorpions are highlighted by their medical importance, and for being their venom a source of several molecules with biological and pharmacological activity not yet fully understood, including several bioactive peptides. Antimicrobial peptides (AMPs) are components of the immune system in prokaryotes and eukaryotes, used in the first line of defense against microorganisms. In the present study, we characterized the first PAM previously identified through transcriptome of the venom gland of the scorpion Tityus stigmurus, named Stigmurin. The characteristics of Stigmurin were investigated by computational modeling and construction of dendrogram. In vitro tests investigated the antibacterial, antifungal, haemolytic and cytotoxic effects of crude venom and Stigmurin. In addition, the structural characteristics of Stigmurin were investigated by circular dochroism in water, 2, 2 , 2- trifluoethanol (TFE) and sodium dodecyl sulfate (SDS) and the models were refined by molecular dynamics simulations. The results showed that the selected sequence encodes a mature protein of 17 amino acid residues and the dendrogram reveals a case of convergent evolution. The crude venom showed no antimicrobial activity, however, Stigmurin exhibited a broad spectrum of antibacterial activity, with minimal inhibitory concentrations (MIC) ranging from 31.25 and 250 µg/mL for different strains, while the hemolytic activity at these concentrations was low. In cytotoxicity studies, the crude venom was unable to reduce cell viability in VERO E6 cells; in contrast, its activity in SiHa cells was significantly higher, corresponding to IC50 of 3.6 µg/mL. For Stigmurin the concentration sable to decrease cell viability of Vero E6 and SiHa cells in 50% were 275.67 µg/mL and 212.54 µg/mL, respectively. The dichroism spectra revealed the conformational flexibility, with predominating extended and β–sheet structures, as well as a remark able renaturation ability. The results suggest that Stigmurin could be considered as a potential antiinfective drug

Borboletas no semiárido: sazonalidade e padrões de diversidade de borboletas frugívoras em um ambiente extremo

The spatial and temporal distribution of the population reflects the adjustment of their biological characteristics to environmental conditions and biotic interactions as adaptive and phylogenetic precursors elements. The habitat’s heterogeneity and alternating seasons tend to cause patterns of activity of organisms and species diversity. However, these seasonal and spatial patterns in butterfly communities in dry environments are not yet clear. We studied a community of frugivorous butterflies in ESEC Seridó, in northeastern Brazil, aiming to characterize the guild in semiarid and check the relative contribution of climate and vegetation variables on its composition, diversity and phenofaunistic. The butterflies were sampled monthly during one year, and the distribution of species was associated with structural characteristics of three vegetation types (eg. richness and abundance of tree and shrub species, canopy cover, herbaceous cover, litter) and climatological data (temperature, rainfall and humidity). We captured 9580 individuals of 16 species of butterflies belonging to four subfamilies (Biblidinae, Charaxinae, Nymphalinae and Satyrinae). The richness, abundance and diversity varied in different scales, especially in time, being higher in the rainy season, while the β-diversity and turnover was higher in the dry. The distribution of species mainly followed the changes in humidity, rainfall and vegetation phenology, with no defined boundaries between habitats. The flight period was shared within subfamilies, which should have distinct response to environmental stimuli, as well as respond to the phenology of host plants and have different reproductive strategies. There is even evidence of physiological and behavioral adaptations as seasonal reproduction and aestivation. So there was environmental control over the distribution and diversity of species, with the key role climate Association and vegetation structure in the community of differentiation in the seasons, and the availability and quality of resources on the variation of species abundance in small scales. These results may support the biomonitoring and conservation preserved areas, particularly in environments under human pressure and extreme environmental conditions such as semi-arid.

Influência das alturas iniciais de pasto diferido na recria de cordeiros sobre o desempenho na terminação em confinamento

The objective of this study was to evaluate the productive performance, metabolic and feeding behavior of sheep after ninety days deferred pasture at different heights. The experiment was conducted at Capim Branco experimental farm of the Universidade Federal de Uberlândia. During the period of 90 days, from June to September 2013, forty-eight crossbred lambs Santa Inês x Dorper, divided into groups of four animals, occupied twelve pickets deferred pasture with four initial heights (15 cm, 25 cm, 35 cm and 45 cm). During this period we evaluated the structural characteristics of pasture. For confinement, from September to December 2013, 32 of these animals were used (16 males and 16 females), divided into four bays, separated as pasture were using. The consumption was assessed daily, while biometric measurements were made every 21 days. In relation to gender, there were differences in average daily gain weight. Reviews of feeding behavior occurred at the beginning, middle and end of the experiment for 24 hours. The rumination and leisure activities do not present statistical differences, both initial height of pasture and by period. The time spent on intake was higher during the daytime both treatments starting height as the experimental periods, however, rumination activity was more intense at night. Blood glucose was achieved in five periods of the day, while other metabolites have been obtained with a collection made fortnightly. The different initial heights not promoted effects on blood glucose. The harvesting times were not affected. However, there was a reduction of basal blood glucose of animals throughout the experimental period. There was a significant interaction between the initial pasture heights and periods of evaluation of basal glucose. Cholesterol and triglyceride levels were below recommended levels, however the final phase of confinement cholesterol level increased significantly. The values of VLDL and GGT were above the reference range. FA and AST showed average values within the recommended values. Total protein was influenced by different initial heights of pasture. The creatinine and albumin had values below the recommended range. Moreover, the albumin decreased during the confinement time. Uric acid showed close to the recommended maximum. There was stabilization of compensatory growth, with modification of consumption and weight gain at 45 days of experiment.

Surfactant-mediated variation of band-edge emission in CdS nanocomposites

The optical-structural characteristics of the direct optical band-gap semiconducting series of surfactant template-mediated laminar (CdS)x(CdCl2)y(CnH2n+4N)z nanocomposites are reported. X-ray diffraction measurements of the nanocomposites exhibited interlaminar distances in the range 2.9-3.6 nm with observations of eighth order {0 0 l} diffraction planes indicative of a high degree of laminarity and crystallographic order. Diffuse reflectance measurements have determined that the profile of their emission spectrum is that of a direct band-gap with absorption edges in the range 2.11-2.40 eV, depending on the CdS mole fraction in the nanocomposite. Photoluminescence (PL) excitation and time-resolved PL spectroscopies give an estimate of the maximum relative absorbance of the nanocomposites at ∼420 nm while the minimum was observed at ∼560 nm. The main emission was observed at ∼700 nm with emission from doubly ionized sulphur vacancies observed at ∼615 nm at room temperature. The CdS-containing nanocomposite is thus a surfactant-mediated modular system with variable band-gap energy emission.

Synthesis and characterization of nanocomposites based on PANI and carbon nanostructures prepared by electropolymerization

Nanocomposites based on polyaniline (PANI) and carbon nanostructures (CNSs) (graphene (G) and multiwall carbon nanotubes (MWCNTs)) were prepared by in situ electrochemical polymerization. CNSs were inserted into the PANI matrix by dispersing them into the electrolyte before the electropolymerization. Electrochemical characterization by means of cyclic voltammetry and steady state polarization were performed in order to determine conditions for electro- polymerization. Electro-polymerization of the PANI based nanocomposites was carried out at 0.75 V vs. saturated calomel electrode (SCE) for 40 and 60 minutes. The morphology and structural characteristics of the obtained nanocomposites were studied by scanning electron microscopy (SEM) and Raman spectroscopy, while thermal stability was determined using thermal gravimetric analysis (TGA). According to the morphological and structural study, fibrous and porous structure of PANI based nanocomposites was detected well embedding both G and MWCNTs. Also, strong interaction between quinoidal structure of PANI with carbon nanostructures via π–π stacking was detected by Raman spectroscopy. TGA showed the increased thermal stability of composites reinforced with CNSs, especially those reinforced with graphene.

As Características das Redes Sociais Pessoais de Idosos Segundo o Estado Civil

Objetivos: O presente estudo tem como principal objetivo caraterizar as redes sociais pessoais dos idosos com idade igual ou superior a 65 anos, relativamente às caraterísticas estruturais, funcionais e relacionais-contextuais, analisando-as segundo o estado civil. Metodologia: Para avaliar as variáveis em estudo foram utilizados: o instrumento de Análise da Rede Social Pessoal, versão para idosos (IARSP – Idosos) (Guadalupe, 2010; Guadalupe & Vicente, 2012) com o objetivo de avaliar as dimensões da rede social pessoal dos idosos e um inquérito por questionário para caracterização sociodemográfica. Participantes: A amostra é constituída por 446 idosos com idades compreendidas entre os 65 anos e os 98 anos (M = 76,09; DP = 7,59). Os participantes são na sua maioria do sexo feminino (n = 285, 63,9%). A maioria dos idosos é casada/união de facto (n = 230, 51,6%) e em minoria encontram-se os divorciados/separados (n = 21, 4,7%) e têm filhos (n=389, 87,2%). Resultados: Os resultados demonstram que o estado civil apresenta associações estatisticamente significativas com as variáveis sociodemográficas sexo, idade, viver só, parentalidade e escolaridade. Registam-se diferenças significativas relativamente ao estado civil no que diz respeito à maioria das características estruturais da rede, quanto às características funcionais, nomeadamente o acesso a novos vínculos, a reciprocidade de apoio, a satisfação com a rede e com o suporte social, e quanto às características relacionais-contextuais apenas se assinalam relativamente à distância de residência. Conclusões: O nosso estudo revela que as redes sociais pessoais dos idosos se diferenciam a nível estrutural e funcional segundo o estado civil destes idosos. Os idosos casados apresentam redes maiores mais centradas nas relações familiares na rede do que os idosos com outros estados civis. Os idosos solteiros são os que apresentam redes menores, mais investidas nas relações de amizade e de vizinhança e menos nas relações familiares comparativamente com os outros tipos de relacionamento. / Objectives: This study aims to characterize the personal social networks of the elderly aged 65 years or more, for structural, functional and relational-contextual features, analyzing them according to marital status. Methodology: To assess the variables studied the following was used: the analysis tool of the Personal Social Network, version for elderly (IARSP - Elderly) (Guadalupe, 2010; Guadalupe & Vicente, 2012) in order to assess the dimensions of the personal social network of the elderly and a questionnaire for socio-demographic characterization. Participants: The sample comprises 446 elderly, aged between 65 years and 98 years (M = 76.09, SD = 7.59). Participants are mostly female (n = 285, 63.9%). Most seniors are married / consensual union (n = 230, 51.6%) and a minority is divorced / separated (n = 21, 4.7%) and have children (n = 389, 87. 2%). Results: The results show that marital status has statistically significant associations with the sociodemographic variables, gender, age, living alone, and parenting and education. There are significant differences with regard to marital status relating to most of the structural characteristics of the network, for the functional features, namely access to new links, reciprocal support, satisfaction with the network and social support, and as to the relational-contextual characteristics these only appear in relation to the distance of residence. Conclusions: Our study shows that personal social networks of the elderly are different on a structural and functional level according to the marital status of these seniors. Married elderly have larger networks more centered on family relationships on the network than the elderly with other marital statuses. The single elderly are those with smaller networks, more invested in the relations of friendship and neighborhood and less on family relationships compared to other types of relationship.

Redes Sociais Pessoais e Mobilidade em Idosos

Objetivos: A investigação tem como objetivo geral analisar a associação entre a mobilidade nos idosos e as características estruturais, funcionais e relacionais-contextuais das suas redes sociais pessoais. Metodologia: No nosso estudo utilizámos o Instrumento de Análise da Rede Social Pessoal, IARSP, versão idosos (IARSP Idosos de Guadalupe & Vicente, 2012; Guadalupe, 2009), que permite caracterizar a rede social pessoal do individuo e uma questão do WHOQOL (OMS; Canavarro et al., 2006) para avaliar a perceção de mobilidade. Participantes: A amostra é constituída por 446 idosos com idades compreendidas entre os 65 anos e os 98 anos. Os participantes são na sua maioria do sexo feminino (63,9%; n = 285), casados (51,6%; n = 230) e com escolaridade (65,9%; n = 294). Resultados: Verifica-se que são os idosos do sexo feminino, os casados e com escolaridade, aqueles que apresentam uma média mais elevada de mobilidade percebida. Constata-se que existe uma maior proporção de idosos cuja mobilidade é “boa ou muito boa” (48,2%; n = 215) em comparação com os que têm uma mobilidade “nem boa nem má” (26,5%; n = 118) e com os que a mobilidade é “má ou muito má” (25,3%; n = 113). Quanto às características estruturais, os idosos que apresentam melhor mobilidade percebida têm uma média mais elevada na proporção das relações familiares nas redes (M = 77%), quanto às características funcionais, na companhia social (M = 2,37), apesar de não existirem diferenças significativas, e na reciprocidade de apoio (M = 3,53; p= 0,002), e consideram estar muito satisfeitos com a rede (M = 2,87, p= 0,059), quando tais características comparadas com as dos idosos com pior mobilidade. Verificam-se também diferenças entre as subamostras quer no apoio material e instrumental (M = 2,30; p = 0,013) e no apoio informativo (M = 2,44; p = 0,001), registando o grupo das pessoas com pior mobilidade as médias mais baixas. Quanto às características relacionais contextuais, na durabilidade das relações, os sujeitos com mobilidade “má ou muito má” apresentam uma média mais elevada (M = 42,41 anos; p = 0,025). Conclusão: Segundo a pior ou melhor mobilidade, é nas características funcionais que se assinalam as principais diferenças estatisticamente significativas, o que nos permitem afirmar uma associação entre mobilidade nos idosos e as relações interpessoais, pelo conteúdo das relações e pelo apoio que percecionam. Concluímos que os idosos que se consideram com melhor mobilidade apresentam, na generalidade, características de rede social mais positivas quando comparados com os que têm menor mobilidade percebida. /Objectives: The main goal of the research is to analyze the association between mobility in the elderly and the structural, functional and relational-contextual characteristics of their personal social networks. Methodology: In our study, we used the Social Personal Network Assessment Tool – IARSP , elderly version (IARSP-Idosos of Guadalupe Vicente, 2012; Guadalupe, 2009), enabling an individual's personal social network and a question of WHOQOL (WHO; Canavarro and such, 2006) to evaluate their perception of functionality of mobility. Participants: The study has 446 elderly participants aged between 65 years and 98 years. Participants are mostly female (63,9%; N = 285), married (51,6%; N = 230) and with education (65,9%; N = 294). Results: We found that older women, married and with education are those with a better mobility. It is noted that there is a greater proportion of elderly people whose mobility is "good or very good" (48.2%, n = 215) compared to those with a mobility "neither good nor bad" (26.5%, n = 118) and the mobility is "bad or very bad" (25.3%; n = 113). Regarding the structural characteristics, seniors who have better perceived mobility have a higher average in the proportion of family relationships in networks (M = 77%; p 0,331), and for the functional characteristics as social company (2, 37; p = 0,418), reciprocal support (3.53; p = 0.002), and satisfaction with their network (M = 2.87; p = 0.059) when these characteristics are compared with those of older people with poor mobility. Exist also statistically significant differences between subsamples either in the material and instrumental support (M = 2.30; p = 0.013) and informational support (M = 2.44; p = 0.001), recording the group of people with poorest mobility. For the relational and contextual characteristics, individuals with mobility "bad or very bad" have a higher mean durability of relationships (M = 42.41; p = 0.025). Conclusion: According to better or worse mobility, the functional characteristics indicates major differences between subsamples, that allow us to assert an association between mobility in the elderly and the content of interpersonal relationships, as well as the perception of support. We conclude that the elderly who consider themselves to have better mobility in general, have more positive social networking features when compared to those with lower perceived mobility.

Participação Social e Redes Sociais Pessoais de Idosos

Objetivo: O objetivo central deste estudo é caracterizar as redes sociais pessoais de indivíduos com idade igual ou superior a 65 anos, a nível estrutural, funcional e relacional-contextual, analisando-as segundo o nível de participação social dos idosos ao longo da sua vida em estruturas comunitárias ligadas ao lazer, cultura, desporto, religião e voluntariado. Metodologia: Para a avaliação das variáveis em estudo foram utilizados o Instrumento de Análise da Rede Social Pessoal, versão para idosos (IARSP – Idosos) (Guadalupe, 2010; Guadalupe & Vicente, 2012) para avaliar as dimensões da rede social pessoal, um questionário para caracterizar as variáveis sociodemográficas e a participação social e a Satisfaction With Life Scale – SWLS (Diener, 1985) que permite avaliar o grau de satisfação com a vida. Participantes: A amostra é constituída por 567 idosos, com uma média de idades de 75 anos (DP=7,6), entre os 65 anos e os 98 anos, maioritariamente do sexo feminino (63,0%), casados ou em união de facto (53,7%) e com escolaridade (69,8%), sobretudo ao nível do quarto ano (51,3%). A maioria dos idosos inquiridos não vive só (79,4%) numa zona de residência maioritariamente inserida em aglomerado populacional em região rural (57,0%) e não usufrui de qualquer tipo de apoio de resposta social (75,5%). Resultados: A amostra divide-se entre os que participaram comunitariamente ao longo da vida (47,8%; n = 271) e os que não participaram (52,2%; n = 296), sendo que entre os que participam 16,7% fazem-no com elevada frequência. Os idosos do sexo feminino, com idade igual ou inferior a 75 anos, casados, com habilitações literárias e que vivem acompanhados, são os que têm uma maior probabilidade de ter uma participação social mais ativa. Os idosos que apresentam participação social têm uma rede maior, com um membro a mais em média (M = 8,52 vs. 7,51, p = 0,027), e uma composição distinta dos que não participam, com menor peso das relações familiares (M = 72,61% vs. 80,81%, p < 0,001), maior peso e mais relações de amizade (M = 15,43% vs. M = 9,24%, p < 0,001) e maior presença de relações de trabalho (M = 1,11% vs. 0,13%, p = 0,006). Relativamente às características funcionais, podemos constatar que a reciprocidade de apoio é percebida como maior (p = 0,010) entre os idosos que participam comunitariamente, não se verificando diferenças noutras variáveis funcionais e relacionais-contextuais. O nível de participação e a satisfação com o nível de participação correlacionam-se positivamente com a satisfação percebida com a vida (p < 0,001). Conclusão: As conclusões apontam para um efeito da participação social ao longo da vida em estruturas comunitárias nas características estruturais das redes sociais pessoais dos idosos, não se verificando interferência na maioria das características funcionais e nas relacionais-contextuais. Verificámos ainda que há uma associação entre a participação social e a satisfação com a vida, sendo mais satisfeitos os que participam em estruturas comunitárias. É possível constatar que a rede daqueles que referem ter participação social é tendencialmente maior e heterogénea na composição, quando comparada com as redes dos sem participação social, assumindo, assim, relevância na estruturação de uma rede mais diversa e ampla, devendo ser estimulada no sentido de promover uma rede com recursos potencialmente positivos e um envelhecimento mais ativo. / Objectives: The central objective of this study is to characterize the personal social networks of the elderly, aged 65 years or more, analyzing them according to the level of social participation throughout their life in community structures related to leisure, culture, sports, religion and volunteering. Methodology: For the evaluation of the variables we used the Social Network Analysis Tool (IARSP-elderly) (Guadalupe, 2010; Guadalupe Vicente, 2012) to assess the dimensions of the social network; a questionnaire to evaluate social participation; and the Satisfaction With Life Scale SWLS – (Diener, 1985) to acess the degree of satisfaction with life. Participants: The sample consists of 567 elderly, with an average age of 75 years old (SD = 7,595), between 65 and 98 years old, mostly female (63.0 %), married (53.7%) with education (69.8%), mainly with the 4th grade (51.3%). Most of the respondents do not live alone (79.4%) in agglomerations in rural region (57.0%) and are not users of social services (75.5%). Results: The sample is divided between those who had community participation throughout life (47.8 %; n = 271) and those who did not participated (52,2%; n = 296). Between the first, 16.7% do it with high frequency. The elderly women, aged less than 75 years old, married, with educational qualifications and living not alone, are those who have a higher likelihood of having a more active social participation. The elderly that present social participation have a larger network, with one more member (M = 8,52 vs. 7,51, p = 0,027), and a composition distinct from not participating, with less proportion of family relations (M = 72,61% vs. 80,81%, p < 0,001), greater proportion and more friendships (M = 15,43% vs. M = 9,24%, p < 0,001) and greater presence of working relations (M = 1,11% vs. 0,13%, p = 0,006). Regarding the functional dimension, the reciprocity of support is perceived as higher (p = 0.010) among seniors participating in community and there were no differences in other functional and relational-contextual variables. The level of participation and satisfaction with the level of participation correlate positively with perceived satisfaction with life (p <0.001). Conclusion: The findings point to an effect of lifelong social participation in community in structural characteristics of personal social networks of the elderly, not verifying interference in most of the functional and the contextual-relational characteristics. We have also found that there is an association between social participation and life satisfaction, being more satisfied when they participate in community structures. The social network of the elderly who reported having social participation tends to be larger and heterogeneous in composition compared with those without social participation, thus assuming importance in structuring a more diverse and extensive network, should be encouraged in order to promote a network with potentially positive resources and a more active aging.

Satisfação com a Vida e Redes Sociais Pessoais de Idosos

Objetivos: A investigação tem como objetivo geral analisar a associação entre a satisfação com a vida nos idosos e as características estruturais, funcionais e relacionais-contextuais das suas redes sociais pessoais. Metodologia: A recolha dos dados foi feita através de um questionário sociodemográfico, da Satisfaction With Life Scale – SWLS (Diener, 1985) que permite avaliar o grau de satisfação com a vida e também do Instrumento de Avaliação da Rede Social Pessoal – IARSP – Idosos (Guadalupe, 2009; Guadalupe & Vicente, 2012) que possibilita a análise das características das redes sociais pessoais dos idosos. Participantes: A amostra é constituída por 416 idosos com idades compreendidas entre os 65 anos e os 98 anos (M = 76,15; DP = 7,584). Os participantes são de ambos os sexos, mas na sua maioria do sexo feminino (63,7%; n = 265). A maioria dos sujeitos da amostra é casada (51,2%; n = 213) e tem escolaridade (64,2%; n = 267). Resultados: Verifica-se que são as mulheres idosas, os casados e com escolaridade que percebem uma maior satisfação com a vida. Constata-se que existe uma maior proporção de idosos satisfeitos com a vida (53,8%; n = 83) em comparação com os medianamente satisfeitos (26,2%; n = 109) e com os insatisfeitos (20%; n = 83). Os idosos que apresentam uma maior satisfação percebida têm uma média mais elevada na proporção das relações familiares nas redes (M = 80,67), no apoio emocional, material e instrumental, informativo, companhia social, e no acesso a novos vínculos, e consideram estar muito satisfeitos tanto com a rede (M = 2,92) como com o apoio que esta disponibiliza (M = 2,77). Conclusão: Verificaram-se associações estatisticamente significativas nas características estruturais da rede, no entanto são as características funcionais que atestam os principais resultados que nos permitem afirmar uma associação entre satisfação com a vida nos idosos e as relações interpessoais, pelo conteúdo das relações e pelo apoio que percecionam. Concluímos que os idosos que se consideram satisfeitos com a vida apresentavam, na generalidade, características de rede social mais positivas quando comparados com os que percebiam menor satisfação com a vida. / Objectives: The research has as main objective to analyze the association between life satisfaction in the elderly and the structural, functional and relational-contextual characteristics of their personal social networks. Methodology: The data collection was done through a sociodemographic questionnaire, the Satisfaction With Life Scale – SWLS (Diener, 1985) that evaluates the degree of satisfaction with life and also the Personal Social Network Analysis Tool – IARSP – Elderly (Guadalupe, 2009; Guadalupe & Vicente, 2012) for the analysis of personal social networks’ characteristics. Participants: The sample consisted of 416 elderly aged between 65 and 98 years (M = 76,15; SD = 7,584). Participants are both genders, but mostly female (63,7%; n =265). Most of the participants are married (51,2%; n = 213) and have education (64,2%; n = 267). Results: It is found that are the older women, married and with education that perceive a greater satisfaction with life. There is a greater proportion of elderly satisfied with the lives (53,8%; n = 83) compared to the moderately satisfied (26,2%; n = 109) and the unsatisfied (20%; n = 83). Seniors who have a higher satisfaction have a higher average in the proportion of family relationships in their networks (M = 80,67), emotional support, material and instrumental, informational, social companionship, and access to new bonds, and consider to be very satisfied with their social network (M = 2,92) and with the social support that it provides (M = 2,77). Conclusion: There were statistically significant associations in the structural characteristics of the network, however are the functional characteristics that allow us to state an association between life satisfaction in the elderly through interpersonal relationships, the content of relations and the support they perceive. We conclude that elderly people who consider themselves satisfied with life showed, in general, have more positive characteristics of social personal network when compared to those who perceive less satisfaction with life.

Redes Sociais Pessoais de Idosos com e sem Filhos

Objetivos: O presente estudo tem como objectivo analisar as redes sociais e pessoais de idosos portugueses com filhos e sem filhos, relativamente às características estruturais, funcionais e relacionais-contextuais. Metodologia: Para a avaliação das variáveis em estudo foram utilizados: o Instrumento de Análise da Rede Social Pessoal, versão para idosos (IARSP – Idosos) (Guadalupe, 2010; Guadalupe & Vicente, 2012) para avaliar as dimensões da rede social pessoal; um inquérito por questionário para caracterização da amostra a nível sociodemográfico e sociofamiliar. Participantes: A amostra é constituída por 418 idosos, com uma média de idades de 76 anos (DP=7,62), entre os 65 anos e os 98 anos, maioritariamente do sexo feminino (63,9%), casados ou em união facto (51,0%) e com escolaridade (63,9,1%). A maioria dos idosos inquiridos vive com agregado familiar (80,4%) em zonas rurais (64,8) e não usufrui de qualquer tipo de apoio de resposta social (71,5%). Resultados: Os idosos com filhos são maioritários (n=364; 87,1%) e os sem filhos os minoritários (n=54; 12,9%) na nossa amostra. Dos que têm filhos, 29,9% (109) têm filhos únicos e 70,1% (255) têm mais do que um filho (67% com 1 ou 2 filhos), sendo os filhos maioritariamente de ambos os sexos (n=158; 43,4%). Os resultados sugerem que o facto de os idosos terem ou não terem filhos reflete-se sobretudo em diferenças nas características estruturais das redes. Os idosos com filhos apresentam uma média mais elevada no tamanho da rede, na proporção das relações de familiares, e satisfação da rede e reciprocidade de apoio (p ≤ 0,001), a nível das características funcionais. Já relativamente à proporção das relações de amizade, vizinhança na rede, são os idosos sem filhos que apresentam uma média substantivamente mais elevada (p ≤ 0,003) do que a outra subamostra, apresentando também uma maior durabilidade das relações interpessoais (p < 0,03). As correlações entre o número total de filhos/as com as características estruturais e funcionais da rede, indicam-nos que quanto maior é o número de filhos maior é o tamanho da rede, a proporção das relações familiares, o apoio emocional, a reciprocidade de apoio, a satisfação com a rede e com o suporte social. Conclusões: O nosso estudo revela que as redes sociais pessoais dos idosos diferenciam-se a nível estrutural segundo o facto de terem ou não filhos, mas também nalgumas variáveis funcionais. Os idosos com filhos apresentam redes mais alargadas e muito centradas nas relações familiares na rede (sensivelmente menos um terço) do que os idosos sem filhos. Os últimos apresentam redes menores (com cerca de 2 pessoas a menos) e mais investidas nas relações de amizade e de vizinhança (com proporções que representam mais do dobro destes vínculos). / Goals: The present study has the purpose to analyze the personal social networks of Portuguese elder with our without sons/daughters, in their structural, functional and contextual relations. Methodology: We used for the evaluation of the variables: The Personal Social Network Analysis Tool, regarding elderly people (IARSP – elderly people) (Guadalupe, 2010; Guadalupe & Vicente, 2012), to evaluate the personal social network dimensions; a survey through an inquiry to characterize the sample at sociodemographic and at sociofamily level. Participants: The sample includes 418 elderly with an average age of 76 years old (DP = 7,62) between 65 and 98 years old, mainly women (63,9%), married or living as a couple (51,0%) with education (63,9,1%). Most of the elderly people who answered the survey live in family household (80,4%) in rural areas (64,8) and do not benefit from any type of social services support (71,5%). Results: The majority is elderly people with sons/daughters (n=364, 87,1%) and the ones without are the minority (n= 54, 12,9%) in our sample. Within those who are parents, 29,9% (109) have only one child and 70,1% (255) have more than one child (67% with 1 or 2), and the children are mainly of both sexes (n= 158; 43,4%). The results suggest that the fact that the elderly have or not have children is reflected especially in differences in the structural characteristics of the networks. Elderly people with children have a higher average in the network size, in the proportion of family relationships, and satisfaction of the network and reciprocal support (p ≤ 0,001). Regarding the proportion of friendly relations, network neighborhood, are the elderly without children who have a substantially higher mean (p ≤ 0,003) than the other subsample, also featuring improved durability of interpersonal relationships (p < 0,003). The correlations between the total number of children/with the structural and functional characteristics of the network, indicate that the greater the number of children, the greater the size of the network, the proportion of family relationships, emotional, support, reciprocity support and satisfaction with social network. Conclusion: Our study reveals that the personal social networks of the elderly differ in the structural level, according to whether they have sons/daughters or not, but also in some functional variables. The elderly with children have networks much more broader and centered on family relationships (roughly one-third) than elderly without children. These elderly without children have smaller networks (about 2 fewer people) and more centered in relations of friendship and neighborhood (with proportions that are more than the double compared to the other subsample).

Quantifying and Modeling the Influence of Forest on the Magnitude and Duration of Mountain Snow Storage in the Pacific Northwest, USA

Thesis (Ph.D.)--University of Washington, 2016-08

The Impact of Light Rail Transit-Oriented Development on Residential Property Value in Seattle, WA

Thesis (Master's)--University of Washington, 2016-06

Chitosan scaffold as an alternative adsorbent for the removal of hazardous food dyes from aqueous solutions

Hypothesis: The dye adsorption with chitosan is considered an eco-friendly alternative technology in relation to the existing water treatment technologies. However, the application of chitosan for dyes removal is limited, due to its low surface area and porosity. Then we prepared a chitosan scaffold with a megaporous structure as an alternative adsorbent to remove food dyes from solutions. Experiments: The chitosan scaffold was characterized by infrared spectroscopy, scanning electron microscopy and structural characteristics. The potential of chitosan scaffold to remove five food dyes from solutions was investigated by equilibrium isotherms and thermodynamic study. The scaffold–dyes interactions were elucidated, and desorption studies were carried out. Findings: The chitosan scaffold presented pore sizes from 50 to 200 lm, porosity of 92.2 ± 1.2% and specific surface area of 1135 ± 2 m2 g 1. The two-step Langmuir model was suitable to represent the equilibrium data. The adsorption was spontaneous, favorable, exothermic and enthalpy-controlled process. Electrostatic interactions occurred between chitosan scaffold and dyes. Desorption was possible with NaOH solution (0.10 mol L 1). The chitosan megaporous scaffold showed good structural characteristics and high adsorption capacities (788–3316 mg g 1).

Avaliação do potencial anti-inflamatório, anticoagulante e hemorrágico de heparinóides presentes em "Artemia franciscana"

Heparan sulfate (HS) and Heparin (Hep) glycosaminoglycans (GAGs) are heterogeneous and highly charged polysaccharides. HS is structurally related to Hep but is much less substituted with sulfo groups than heparin and has a more varied structure (or sequence). Because of structural similiarities between these two polymers, they have been described together as heparinoids . Both chains bind a variety of proteins and mediate various physiologically important processes including, blood coagulation, cell adhesion and growth factor regulation. Heparinoids with structural characteristics similar to these described from HS and/or Hep from mammalian tissues have been isolated from different species of invertebrates, although only a few heparinoids from unusual sources have been characterized. The present study describes the presence of unusual heparinoids population from Artemia franciscana, isolated after proteolysis and fractionation by ion exchange resin and named, F-3.0M. The study model in vivo were hemostasis (rat tail scarification) and inflamatoty activity. The tests in vitro were used for coagulations assays (PT and APTT). The analyse of the heparinoids eluted with 3,0M NaCl showed electrophoretic migration in different buffer systems a single band with a behaviour intermediate between those of mammalian HEP and HS. The main products obtained from Artemia heparinoids after enzymatic degradation with heparitinases I and II from F. heparinum were N-sulphated disaccharides (∆U-GlcNS,6S/ ∆U,2S-GlcNS and ∆U-GlcNS) and N-acetylated disaccharides (∆U, GlcNAc). This heparinoid had a lower hemorrhagic effect (400μg/ml) when compared to unfractiionated heparins(25μg/ml).The results also suggest a negligible APTT activity of this heparinoid (62.2s). No action was observed on PT indicating that F-3.0M haven t action on the extrinsic pathway. The results showed that the fraction F- 3.0M have inhibitory effect on migration of leukocytes, 64.5% in the concentration of 10 μg/ml (P<0.001). The search for new heparin and/or heparan sulphates analogs devoid of anticoagulant activity is an atractive alternative and may open up a wide variety of new therapeutic applications