Detecção de microrganismos de infecções bucais: perspectivas e cuidados a serem seguidos

The diagnosis of head and neck infections constitutes relevant step in their treatment. However, in spite of the fact that most of diseases in head and neck region are infectious in nature, several reasons collaborated for dentists do not ask laboratory tests in order to help clinical diagnosis. By mean of this review literature, based on research articles about the newest and most reliable methods of diagnosis for clinical laboratories, the authors discuss the advantages and disadvantages of each selected method and the relevant aspects in transportation of the specimens to the laboratory. Saliva, biofilm, pus, and blood are the most frequent specimens for microbial diagnosis, being that the most used methods are culture and those based on detection of deoxyribonucleic acid by polymerase chain reaction method. Whereas, the culture depends on cellular viability, and has reduced sensitivity, as well as needs favorable conditions in the sample collection and transportation, PCR shows high sensitivity and specificity, but it does not allow the determination of antibiogram, what reduces its usefulness. In addition, few laboratories possess conditions to perform cultivation of obligate anaerobes or have experience in the molecular detection of these microorganisms.

Antimicrobial activity of six plant extracts from the brazilian savanna on periodontal pathogens

Fundação do Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Classe mollicutes na boca de pacientes com dependência química

Drug addiction is one of the biggest public health problems worldwide, not only by the dimensions of the problem, but also by the severity of the damage, creating favorable conditions for opportunistic microorganisms such as class Mollicutes. This study aims to evaluate the presence of the main species and genera of this group in the subgingival biofilm of drug addiction patients, comparing them with non-dependent subjects. For this purpose, data on systemic health conditions, socioeconomic characteristics, drug addiction from 72 patients with drug addiction kept in rehab clinics and 100 non-dependent patients who formed the control group were obtained. Intra and extraoral clinical examinations were performed and samples of subgingival plaque were collected through sterile absorbent paper cones. The presence of different genera and species of the class Mollicutes was evaluated by PCR using the specific primers and conditions for each microorganism. The statistical analysis was performed using the Chi-square test for comparisons of three or more variables and the Mann-Whitney test, with significance level of 5%. Out of species and genera evaluated, Mycoplasma salivarium showed correlation with gingival inflammation in both patient groups and was more frequently detected among drug addiction patients

Quimiluminescência como fonte de luz alternativa na terapia fotodinâmica

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Surtos de gastroenterite relacionados ao consumo de laticínios no estado de São Paulo no período de 2000 a 2010

Milk is considered a nutritionally complete food but it is also an excellent substrate for microbial growth. Therefore, it may pose a risk to consumers’ health. The aim of this study was to collect data on the occurrence of gastroenteritis outbreaks caused by the intake of milk and dairy products in the state of São Paulo, from 2000 to 2010. The data were collected from the records on foodborne illness outbreaks kept by the Division of Waterborne and Foodborne Diseases of the Center for Epidemiological Surveillance of the Department of Health of the State of São Paulo. A total of 239 outbreaks were notifi ed. They involved 2.418 cases related to the consumption of dairy products during the study period. The etiological agents were identifi ed in 79 outbreaks (33.0%). They were mostly bacteria (84.8%), especially Staphylococcus aureus. Most outbreaks occurred at home (59.4%) and during the months of January (14.2%) and July (12.5%). Most (24.1%) of the affected individuals aged 20 to 49 years. The results shows the need of better manufacturing and handling practices, as well as increasing the awareness of the population and relevant agencies about the importance of notifying cases and implementing preventive measures to ensure food safety.

Relação da diversidade bacteriana e quantidade de endotoxinas com a sintomatologia dolorosa de pulpite reversível em lesões cariosas profundas

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Virginiamycin via mineral supplementation and sward height in growing Nelore young bulls

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Ciclagem de carbono em área sob semeadura direta e aplicação de lodo de esgoto

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Orquiepididimite causada por Corynebacterium sp e Proteus mirabilis em cordeiro da raça Lacaune: relato de caso

Orchiepididymitis is the inflammation of both, testis and epididymis and may be caused by microbial agents such as Brucella ovis. Thus, the aim of the present report is to present a case of orchiepididymitis in a Lacaune ram with unilateral testicular enlargement. Ultrasonography of the testis revealed the presence of a circular hypoechogenic structure adjacent to the testicular parenchyma. Corynebacterium sp was isolated from the surgically removed testis. Two months after removal of the affected testis, the animal returned to the Veterinary Hospital due to an enlargement of the remaining testis. A new ultrasonographic exam revealed the presence of abscesses in the testicular parenchyma and periorchitis. After orchiectomy, Proteus mirabilis was isolated from the material.

Produção semissólida de metarhizium anisopliae e beauveria bassiana em diferentes substratos e efeito da radiação ultravioleta e da temperatura sobre propágulos desses entomopatógenos

The present study was aimed to evaluate different (semi-solid) media for the production of Metarhizium anisopliae and Beauveria bassiana propagules, and to evaluate the tolerance of these propagules to ultraviolet radiation and temperature. The experiments were performed at the Biological Control Laboratory of the Instituto Biológico at Campinas, São Paulo, Brazil. For both fungi, 6 repetitions were performed for each of the 17 treatments: corn starch, full rice, parboiled rice, type-1 rice, type-2 rice, oat flakes, canjiquinha [grits], wheat flour, raw cassava flour, yellow corn flour, special wheat flour, corn flour, corn in grains, cassava starch, soy in grains, crushed wheat, and turf. The viability analysis was done in plastic plates containing BDA. For the bioassays involving exposure to ultraviolet light and temperature, BDA was also used for viability analysis, and each treatment was exposed to the UV radiation for 0, 25 and 50 seconds, the temperature exposure being at 20, 25, 30 and 35º C. Using a Potter tower, 2 mL of fungus suspension from each treatment was inoculated to the Diatraea saccharalis caterpillars. Regarding the sporulation, the largest concentrations of M. anisopliae and B. bassiana were found for the treatments with parboiled rice, type-1 rice, type-2 rice, yellow corn flour, corn flour and crushed wheat. The viability of all treatments was superior to 94.00%. Also, the longer the duration of the exposition to the UV, the smaller the number of fertile conidia. At 35o C, a significant loss of conidia viability was observed, and all the treatments presented some level of virulence.

Biomassa do fungo filamentoso Lasiodiplodia theobromae: composição química e utilização como nutriente para bioprocesso

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Influência do suco de laranja na microbiota intestinal humana

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Remoção de elementos potencialmente tóxicos em resíduo sólido da indústria canavieira para utilização como condicionador de solo na agricultura

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Desenvolvimento de um embutido cárneo fermentado, com teores reduzidos de gordura e sais de cura, através da utilização de culturas probióticas

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Comportamento do carbono orgânico e de atributos químicos, físicos e microbiológicos de um solo arenoso em área de conversão pastagem - Eucalipto

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)