PKC-dependent activation of p46/p54 JNKs during ischemic preconditioning in conscious rabbits.

A conscious rabbit model was used to study the effect of ischemic preconditioning (PC) on stress-activated kinases [c-Jun NH(2)-terminal kinases (JNKs) and p38 mitogen-activated protein kinase (MAPK)] in an environment free of surgical trauma and attending external stress. Ischemic PC (6 cycles of 4-min ischemia/4-min reperfusion) induced significant activation of protein kinase C (PKC)-epsilon in the particulate fraction, which was associated with activation of p46 JNK in the nuclear fraction and p54 JNK in the cytosolic fraction; all of these changes were completely abolised by the PKC inhibitor chelerythrine. Selective enhancement of PKC-epsilon activity in adult rabbit cardiac myocytes resulted in enhanced activity of p46/p54 JNKs, providing direct in vitro evidence that PKC-epsilon is coupled to both kinases. Studies in rabbits showed that the activation of p46 JNK occurred during ischemia, whereas that of p54 JNK occurred after reperfusion. A single 4-min period of ischemia induced a robust activation of the p38 MAPK cascade, which, however, was attenuated after 5 min of reperfusion and disappeared after six cycles of 4-min ischemia/reperfusion. Overexpression of PKC-epsilon in cardiac myocytes failed to increase the p38 MAPK activity. These results demonstrate that ischemic PC activates p46 and p54 JNKs via a PKC-epsilon-dependent signaling pathway and that there are important differences between p46 and p54 JNKs with respect to the subcellular compartment (cytosolic vs. nuclear) and the mechanism (ischemia vs. reperfusion) of their activation after ischemic PC.

Interaction with calmodulin is important for the secretion of thimet oligopeptidase following stimulation

Thimet oligopeptidase (EC 3.4.24.15; EP24.15) was originally described as a neuropeptide-metabolizing enzyme, highly expressed in the brain, kidneys and neuroendocrine tissue. EP24.15 lacks a typical signal peptide sequence for entry into the secretory pathway and is secreted by cells via an unconventional and unknown mechanism. In this study, we identified a novel calcium-dependent interaction between EP24.15 and calmodulin, which is important for the stimulated, but not constitutive, secretion of EP24.15. We demonstrated that, in vitro, EP24.15 and calmodulin physically interact only in the presence of Ca(2+), with an estimated K(d) value of 0.52 mu m. Confocal microscopy confirmed that EP24.15 colocalizes with calmodulin in the cytosol of resting HEK293 cells. This colocalization markedly increases when cells are treated with either the calcium ionophore A23187 or the protein kinase A activator forskolin. Overexpression of calmodulin in HEK293 cells is sufficient to greatly increase the A23187-stimulated secretion of EP24.15, which can be inhibited by the calmodulin inhibitor calmidazolium. The specific inhibition of protein kinase A with KT5720 reduces the A23187-stimulated secretion of EP24.15 and inhibits the synergistic effects of forskolin with A23187. Treatment with calmidazolium and KT5720 nearly abolishes the stimulatory effects of A23187 on EP24.15 secretion. Together, these data suggest that the interaction between EP24.15 and calmodulin is regulated within cells and is important for the stimulated secretion of EP24.15 from HEK293 cells.

Reaction mechanisms in the (6)Li+(59)Co system

The reactions induced by the weakly bound (6)Li projectile interacting with the intermediate mass target (59)Co were investigated. Light charged particles singles and alpha-d coincidence measurements were performed at the near barrier energies E(lab) = 17.4, 21.5, 25.5 and 29.6 MeV. The main contributions of the different competing mechanisms are discussed. A statistical model analysis. Continuum-Discretized Coupled-Channels (CDCC) calculations and two-body kinematics were used as tools to provide information to disentangle the main components of these mechanisms. A significant contribution of the direct breakup was observed through the difference between the experimental sequential breakup cross section and the CDCC prediction for the non-capture breakup cross section. (C) 2009 Elsevier B.V. All rights reserved.

Potential oscillations in a proton exchange membrane fuel cell with a Pd-Pt/C anode

We report in this paper the occurrence of potential oscillations in a proton exchange membrane fuel cell (PEMFC) with a Pd-Pt/C anode, fed with H(2)/100 ppm CO, and operated at 30 degrees C. We demonstrate that the use of Pd-Pt/C anode enables the emergence of dynamic instabilities in a PEMFC. Oscillations are characterized by the presence of very high oscillation amplitude, ca. 0.8 V. which is almost twice that observed in a PEMFC with a Pt-Ru/C anode under similar conditions. The effects of the H(2)/CO flow rate and cell current density on the oscillatory dynamics were investigated and the mechanism rationalized in terms of the CO oxidation and adsorption processes. We also discuss the fundamental aspects concerning the operation of a PEMFC under oscillatory regime in terms of the benefit resulting from the higher average power output. (c) 2010 Elsevier B.V. All rights reserved.

A Computer Vision Framework For Finger-Tapping Evaluation In Parkinson's Disease

Objective: To define and evaluate a Computer-Vision (CV) method for scoring Paced Finger-Tapping (PFT) in Parkinson's disease (PD) using quantitative motion analysis of index-fingers and to compare the obtained scores to the UPDRS (Unified Parkinson's Disease Rating Scale) finger-taps (FT). Background: The naked-eye evaluation of PFT in clinical practice results in coarse resolution to determine PD status. Besides, sensor mechanisms for PFT evaluation may cause patients discomfort. In order to avoid cost and effort of applying wearable sensors, a CV system for non-invasive PFT evaluation is introduced. Methods: A database of 221 PFT videos from 6 PD patients was processed. The subjects were instructed to position their hands above their shoulders besides the face and tap the index-finger against the thumb consistently with speed. They were facing towards a pivoted camera during recording. The videos were rated by two clinicians between symptom levels 0-to-3 using UPDRS-FT. The CV method incorporates a motion analyzer and a face detector. The method detects the face of testee in each video-frame. The frame is split into two images from face-rectangle center. Two regions of interest are located in each image to detect index-finger motion of left and right hands respectively. The tracking of opening and closing phases of dominant hand index-finger produces a tapping time-series. This time-series is normalized by the face height. The normalization calibrates the amplitude in tapping signal which is affected by the varying distance between camera and subject (farther the camera, lesser the amplitude). A total of 15 features were classified using K-nearest neighbor (KNN) classifier to characterize the symptoms levels in UPDRS-FT. The target ratings provided by the raters were averaged. Results: A 10-fold cross validation in KNN classified 221 videos between 3 symptom levels with 75% accuracy. An area under the receiver operating characteristic curves of 82.6% supports feasibility of the obtained features to replicate clinical assessments. Conclusions: The system is able to track index-finger motion to estimate tapping symptoms in PD. It has certain advantages compared to other technologies (e.g. magnetic sensors, accelerometers etc.) for PFT evaluation to improve and automate the ratings

Conciliação: a solução da lide e a celeridade na prestação jurisdicional

Esta obra tem como tema central a conciliação como método contemporâneo de solução dos conflitos, notadamente nas Varas de Família de Porto Velho/RO. Os métodos alternativos de solução de controvérsias surgem como opção eficaz ao jurisdicionado, em atenção ao clamor da sociedade por uma prestação jurisdicional mais célere. A utilização deste método visa oferecer e assegurar uma melhor e efetiva solução de conflitos à população, fugindo assim dos modelos tradicionais de resoluções de conflitos. O objetivo é procurar demonstrar que, além da jurisdição estatal, existem outros meios de pacificação social, os quais também colocam fim às controvérsias e realizam a justiça. Destaque-se que mecanismos alternativos objetivam não somente oferecer uma prestação jurisdicional mais célere à sociedade, mas também verdadeira pacificação social; além do que, oportunizam também a resolução do conflito antes que seja instaurada qualquer demanda judicial, desafogando assim o Judiciário. Enfatiza-se a importância da conciliação nas matérias concernentes ao direito de família, já que nesta área há preocupação fundamental com a preservação emocional das partes. E dada a carga emocional que circundam as ações dessa natureza, muitas vezes ao juiz é impossível encontrar uma resposta adequada e definitiva para os problemas que lhes são apresentados pelas partes. Os resultados demonstram que a conciliação nas Varas de Família de Porto Velho/RO têm se revelado um mecanismo célere e eficaz, na busca da resolução de conflitos e pacificação social. Os profissionais e operadores do direito têm consciência da importância de sua aplicação.

Processos de aprendizagem e suas influências sobre a acumulação de competência tecnológica : o caso da Empresa Enxuta Industrial Ltda.

Esta dissertação é um estudo de caso sobre aprendizagem tecnológica que, considerando as evidências empíricas descritas no Capítulo 6 e à luz da Tabela 3.1, procurou identificar a trajetória de acumulação de competência tecnológica da Enxuta Industrial Ltda., ao longo do período estudado (1980 - 2000), para as funções tecnológicas de investimentos (decisão e controle sobre a planta e engenharia de projetos), de processos e organização da produção, de produtos e de equipamentos. Para construir a estrutura de competências tecnológicas para a indústria de eletrodomésticos foi utilizada a estrutura desenvolvida por Figueiredo (2000). Por outro lado, considerando as evidências empíricas descritas no Capítulo 7 e à luz da Tabela 3.2, procurou-se identificar a influência dos processos de aprendizagem utilizados pela empresa sobre a sua trajetória de acumulação de competência tecnológica. Os processos de aprendizagem são avaliados à luz de quatro características chave: variedade, intensidade, funcionamento e interação, a partir do uso da estrutura desenvolvida por Figueiredo (2000). Este estudo verificou que o modo e a velocidade de acumulação de competência tecnológica na Enxuta Industrial Ltda. foram influenciados pelos processos e mecanismos de aprendizagem usados para adquirir conhecimento tecnológico e convertê-lo em organizacional. Estes processos e mecanismos de aprendizagem propiciaram a empresa alcançar uma maior velocidade na acumulação de capacidades tecnológicas, quando de sua entrada na indústria de eletrodomésticos, o que proporcionou alcançar o desenvolvimento tecnológico necessário para atuar nesta indústria. Porém, a simples incidência desses processos na empresa não garantiu uma implicação positiva para a acumulação de competência tecnológica. Pois, no período de 1991 - 1995, verificou-se a deterioração do funcionamento dos processos de aprendizagem utilizados na empresa, o que pode ter influenciada negativamente a repetibilidade e a interação daqueles processos. Isto parece ter contribuído para que a empresa falhasse em construir e acumular competências tecnológicas inovadoras naquele período. Através do uso de estrutura existente na literatura, aplicada a uma indústria diferente de estudos anteriores, esta dissertação sugere que deve existir um esforço organizado, contínuo e integrado para a geração e disseminação de conhecimento tecnológico na empresa, o que pode acelerar a acumulação de competências tecnológicas, desta forma propiciando à empresa a construção de uma trajetória de aproximação da fronteira de desenvolvimento tecnológica da indústria.

Sistemas de custos em organizações hospitalares: estudo de casos

Esta pesquisa objetivou analisar os sistemas de custos implantados em seis hospitais gerais da cidade do Rio de Janeiro, quanto a sua estrutura e funcionamento, em relação à literatura sobre contabilidade de custos, incluindo os manuais de alguns órgãos governamentais do Brasil. Na revisão de literatura, foram abordados os aspectos e propósitos dos sistemas de custos, as regras para sua implantação, os diferentes sistemas, os métodos de custeio e, finalmente, os enfoques específicos dos sistemas de custos hospitalares (Capítulo II). Para a realização do trabalho, escolheu-se o método de estudo de casos, em razão da carência de verificações empíricas nesta área e a necessidade de uma descrição profunda dos sistemas de custos encontracos (Capítulo III). A seguir, apresentou-se a descrição dos casos, abordando-se as características individuais das unidades hospitalares e a estrutura e o mecanismo dos seus sistemas de custos (Capítulo IV). Os resultados obtidos possibilitaram analisar os tipos de sistemas implantados quanto à natureza dos custos, à forma de acumulação e aos métodos de custeio. Permitiram tambem uma análise dos procedimentos e utilização dos sistemas para fins administrativos (Capítulo V). Relacionando-se os resultados à literatura, chegou-se a importates conclusões e finalmente sugeriu-se novos estudos nesta área.

Política nacional de conciliação: política pública implementação pelo Conselho Nacional de Justiça

Esta obra tem como tema central a abordagem da política nacional de conciliação implementada pelo Conselho Nacional de Justiça, bem como a sua adoção pelo TJRS. Em razão da mudança comportamental da sociedade nas últimas décadas, decorrente de vários fatores, entre eles: a evolução do Estado liberal para o Estado democrático de direito, a constitucionalização dos direitos fundamentais e o acesso à justiça, entre outros, ocorreu o aumento da demanda judicial, gerando problemas na prestação jurisdicional, como morosidade do sistema e difícil acesso ao Poder Judiciário. Assim, no auge da crise do Poder Judiciário, através da emenda constitucional nº 45, foi criado o Conselho Nacional de Justiça, com o objetivo de tornar a prestação jurisdicional, de forma moral, eficiente e efetiva. O Conselho Nacional de Justiça, como integrante do Poder Judiciário, visando, através de uma política pública nacional, a maximizar a prestação jurisdicional e oferecer uma justiça mais célere e justa, através da resolução número 125, determinou a implantação da política Nacional da Conciliação. Desse modo, o Poder Judiciário, utilizando os métodos alternativos de solução de conflitos, a conciliação e a mediação, espera oferecer uma jurisdição mais rápida, contribuir para a pacificação social e diminuir o número de ações judiciais, com a implementação da política Nacional da Conciliação. O Tribunal de Justiça gaúcho, visando a cumprir as determinações da Resolução n. 125 do Conselho Nacional de Justiça, instalou no Estado do Rio Grande do Sul quatro centrais de conciliação e mediação, entre elas a Central Judicial de Conciliação e Mediação da comarca de Pelotas. Os resultados apresentados por esse órgão demonstram que as conciliações, embora não apresentem um número expressivo, têm se revelado um mecanismo célere e eficaz, na busca da resolução de conflitos e pacificação social.

Rent-sharing in Brazil: using trade liberalization as a natural experiment

This paper examines the extent of rent-sharing in Brazil, between 1988 and 1995, combining two different data sets: annual industrial surveys (pIA) and annual household surveys (PNADs). The aim is to use the trade liberalization policies that took place in Brazil in the early 1990s as a "natural experiment" to examine the impact ofproduct market rents on wages. We first estimate inter-industry wage differentials in Brazil, using the household surveys, afier controlling for various observable workers' characteristics. In a reduced form fixed effects equation, these controlled inter-industry differentials are seen to depend on the industries' rate of effective tariff. We also find that LSDV estimates of the effect of value-added per worker (computed using the industrial surveys) on the wage differentials are positive, but somewhat small. However, we find that instrumenting the valued-added with the effective tariffs more than doubles the estimated rent-sharing coefficient. The paper concludes that rent-sharing is prevalent in the Brazilian manufacturing sector, and this mechanism transferred part of the productivity gains due to trade liberalization to manufacturing workers in the form ofhigher (controlled) wage premium.

Desenvolvimento industrial em espaços organizados: uma análise dos fatores de competitividade nos distritos industriais do Estado do Rio de Janeiro

O desenvolvimento industrial no Espaço Organizado (EO) tem se dado através de políticas públicas e iniciativas privadas, atendendo, em alguns casos, aos fatores de competitividade para sua respectiva implementação, outras vezes nem tanto. O principal objetivo desse trabalho foi realizar uma análise dos fatores de competitividade dos distritos industriais do Estado do Rio de Janeiro, contribuindo, dessa forma, através dos resultados apresentados ao final do trabalho, para subsidiar novas políticas públicas e/ou suporte às iniciativas privadas, quando da atração, implantação, retenção e ampliação de indústrias e empresas correlacionadas, nesses espaços. A delimitação da amostra dos Espaços Organizados (EOs) foi definida em função da disponibilidade dos dados e homogeneidade da amostra, no caso, distritos industriais no Estado do Rio de Janeiro. Na execução das etapas do projeto para o cumprimento do seu objetivo principal, foram realizadas etapas intermediárias na seguinte forma: identificação da nomenclatura disponível para os tipos de EOs existentes; elaboração de proposta para a caracterização dos EOs; identificação dos fatores de competitividade dos EOs existentes no Estado do Rio de Janeiro; validação desses fatores de competitividade junto ao grupo entrevistado de representantes do governo, municípios, instituições de fomento e executivos das empresas em operação nas áreas estudadas; mapeamento dos EOs existentes no Estado do Rio de Janeiro e análise dos fatores de competitividade desses espaços, com suas correlações quanto a geração de emprego e atração de empresas. Por fim, após análise dos resultados, a discussão e as considerações finais apontaram tendências que poderão ser aprofundadas em futuros trabalhos de apoio na elaboração das estratégias públicas e/ou privadas que visam melhor aproveitamento e utilização dos EOs destinados ao desenvolvimento industrial.

Why has productivity growth stagnated in most Latin American countries since the neo-liberal reforms?

Latin America’s economic performance since the beginning of neo-liberal reforms has been poor; this not only contrasts with its own performance pre-1980, but also with what has happened in Asia since 1980. I shall argue that the weakness of the region’s new paradigm is rooted as much in its intrinsic flaws as in the particular way it has been implemented. Latin America’s economic reforms were undertaken primarily as a result of the perceived economic weaknesses of the region — i.e., there was an attitude of ‘throwing in the towel’ vis-à-vis the previous state-led import substituting industrialisation strategy, because most politicians and economists interpreted the 1982 debt crisis as conclusive evidence that it had led the region into a cul-de-sac. As Hirschman has argued, policymaking has a strong component of ‘path-dependency’; as a result, people often stick with policies after they have achieved their aims, and those policies have become counterproductive. This leads to such frustration and disappointment with existing policies and institutions that is not uncommon to experience a ‘rebound effect’. An extreme example of this phenomenon is post-1982 Latin America, where the core of the discourse of the economic reforms that followed ended up simply emphasising the need to reverse as many aspects of the previous development (and political) strategies as possible. This helps to explain the peculiar set of priorities, the rigidity and the messianic attitude with which the reforms were implemented in Latin America, as well as their poor outcome. Something very different happened in Asia, where economic reforms were often intended (rightly or wrongly) as a more targeted and pragmatic mechanism to overcome specific economic and financial constraints. Instead of implementing reforms as a mechanism to reverse existing industrialisation strategies, in Asia they were put into practice in order to continue and strengthen ambitious processes of industrialisation.

Avaliação da presença de osteopatia decorremte do diabetes tipo 1 em criança e adolecentes do Rio Grande do Norte

Diabetes Mellitus (DM) and osteoposes are chronic diseases with great socioeconomic consequences, mainly due to the late complications and consequent disabilities. The potential effects of DM on bone metabolism remain a very conroversial issue, and disagreement exists with regard to the clinical implications of diabetic osteopenia and the mechanism of its ocurrence. The issue is further complicated by the contribuicion of the especific factors, such as duration of disease an dthe degree of metabolic control. The objective of this study is to identify the osteopathy in children and adolescents with DM 1 assisted in the hospital of pediatrics, UFRN, through biochemical markers of bone and mineral metabolism and the extent of bone mineral density. The study was composed by 74 diabetics type 1 patients (DM1) of both gender and aged 6 to 20 yars. Normoglicêmic group was composed by 97 healthy subjects of both genders, which showed the same age range of DM1, in addition to same socioeconomic class. These individuals qere students from the networks of public education in the city of Natal-RN, randomly invited to paticipate in our study. Both groups DM1 and NG were divided intofour subgroups, according to the classification of tanner , T1, T2, T3, T4 for achieving a benchmark. Diabetic individuals showed up with a poor glycemic control. the group DN1 T4 showed an incresead value for total protein, albumin, urea and microalbumiuria are predictors of grumelura injury in DM1 patients . The total alkaline phosphatase activitywas kept on high levels for both groups because they are in a stature development age. For osteocalcin there were decreased levels for groups Dm1 T1, T2, and T3 when compared to their NG (s), suggesting that this decrease could be associated with reduction in the number and/or differentiation os osteoblasts thereby contributing to reducing bone formation. There were no changes in the activity of TRAP. The serum concentrations of total and ionized calcium, phosphorus and magnesium were included within the RV. It was observed that the BMD (Z- SCORE ) has always been within the RV for both groups, despite to DM1 T4. Taking all together, our results support the hypothesis that children and adolescents with type 1 DM present the risk in the long run to suffer a reduction in the bone mass, associated to poor glicemic control and disease duration. It could limit the bone growth and increase the probality of development of osteopenia, as well as other complications surch as retinopathy and renal failure

Dispersões sólidas de sinvastatina: preparação, caracterização, no estado sólido utilizando técnicas emergentes e estudo de estabilidade

This thesis aimed to assess the increase in solubility of simvastatin (SINV) with solid dispersions using techniques such as kneading (MA), co-solvent evaporation (ES), melting carrier (FC) and spray dryer (SD). Soluplus (SOL), PEG 6000 (PEG), PVP K-30 (PVP) e sodium lauryl sulphate (LSS) were used as carriers. The solid dispersions containing PEG [PEG-2(SD)], Soluplus [SOL-2(MA)] and sodium lauryl sulphate [LSS-2(ES)] were presented with a greater increase in solubility (5.02, 5.60 and 5.43 times respectively); analyses by ANOVA between the three groups did not present significant difference (p<0.05). In the phase solubility study, the calculation of the Gibbs free energy (ΔG) revealed that the spontaneity of solubilisation of SINV occurred in the order SOL>PEG >PVP 75%>LSS, always 80%. The phase diagrams of PEG and LSS presented solubilization stoichiometry of type 1:1 (type AL). The diagrams with PVP and SOL tend to 1:2 stoichiometry (type AL + AP). The stability coefficients (Ks) of the phase diagrams revealed that the most stable reactions occurred with LSS and PVP. The solid dispersions were characterized by Fourier transform infrared (FTIR), differential scanning calorimetry (DSC), scanning electron microscopy (SEM), particle size distribution (PSD), near-infrared spectroscopy imaging (NIR-CI) and X-ray diffraction of the powder using the Topas software (PDRX-TOPAS). The solid dispersion PEG-2(SD) presented the greatest homogeneity and the lowest degree of crystallinity (18.2%). The accelerated stability study revealed that the solid dispersions are less stable than SINV, with PEG-2(SD) being the least stable, confirmed by FTIR and DSC. The analyses by PDRX-TOPAS revealed the amorphous character of the dispersions and the mechanism of increasing solubility

Trauma em idosos atendidos pelo serviço pré-hospitalar móvel

O The aim of this study was to characterize the occurrence of trauma in the elderly population served by the mobile pre-hospital service, in Natal, Rio Grande do Norte. This is a descriptive, transversal and quantitative approach and whose population consisted of 2,080 trauma victims. The sample, of systematic random type, consisted of 400 elderly people, aged from 60 years old, assisted by the Office of Mobile Emergency in Natal / RN, between January 2011 and December 2012. Data collection began after consent and assent of the institution of a Research Ethics Committee under No. 309 505. It was proceeded to documentary retrospective analysis of records of this service through a form of self-development, validated by expert judges considered reliable (α> 0.75) and valid (CVI = 0.97) in their clarity and relevance. Data were tabulated by the Statistical Package for Social Sciences, version 20.0. The results show that older victims have an average age of 74.19 years old, with a prevalence of female involvement by chronic diseases, especially hypertension, average usage of 2.2 routine medications with vital signs within normal limits. The trauma prevailed during the daytime, in the residence of the victims, north of the city and on weekends. Among the mechanisms of trauma were falls, traffic accidents and physical aggression, whose most common type was brain-cerebral trauma and the main consequences were suture wounds and closed fractures. Basic Support Units were as more driven to pre-hospital care (87.8%) and the main destination place consisted of a referral hospital for emergency of the state (57.5%). Among the most commonly performed procedures by nursing staff immobilization with rigid board and neck collar and the peripheral venipuncture, and the main component used for volume replacement to saline were highlighted. There was a significant relationship between the deaths and the mechanism of injury, mechanism of injury and procedures, except medication administration procedures carried out, except immobilization and unit for service. It is highlighted the prevalence of trauma in the elderly, poor follow-up Pre-Hospital Trauma Life Support protocol and the paucity of records and nursing procedures performed. There is need for a protocol of care specific to elderly trauma victims and education strategies for the prevention of such events