TRATAMENTO DE SEMENTES DE ARROZ, TRIGO, FEIJÃO E SOJA COM UM PRODUTO FORMULADO À BASE DE CÉLULAS E DE METABÓLITOS DE Bacillus subtilis

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

POLIAMINAS E ATIVIDADE da PEROXIDASE em FEIJÃO (Phaseolus vulgaris L.) CULTIVADO SOB ESTRESSE SALINO

O teor de poliaminas (putrescina, espermidina e espermina) e a atividade enzimática da peroxidase (EC 1.11.1.7) foram determinados em plantas de Phaseolus vulgaris L. cv Carioquinha, após terem sido submetidas a estresse salino (50 e 100 mM de NaCl). Foram observadas alterações nos teores das poliaminas, principalmente putrescina, que aumentou com o tempo e a concentração de NaCl. Também ocorreu aumento na atividade da peroxidase em ambas concentrações de NaCl utilizadas. Os resultados mostraram alterações no metabolismo de poliaminas e peroxidases nas plantas de feijão cultivadas em meio salino.

Síntese de acetato de celulose a partir da palha de feijão utilizando N-bromossuccinimida (NBS) como catalisador

Neste estudo a celulose obtida da palha de feijão foi utilizada para produzir um material hidrofóbico (acetato de celulose) para ser avaliado como absorvente de óleo. Nas reações de acetilação foram utilizados anidrido acético e dois catalisadores, a piridina (PY) e N-bromossuccinimida (NBS). Os materiais produzidos foram caracterizados por espectroscopia na região do infravermelho médio, microscopia eletrônica de varredura, difratometria de raios-X e análise elementar. O NBS mostrou-se mais eficiente que a PY e, seu uso resultou em materiais com maiores quantidades de grupos acetatos, mais hidrofóbicos e com maiores capacidades de absorção de óleo de soja.

Influência de fatores antinutricionais da leucena (Leucaena leucocephala e Leucaena cunningan) e do feijão guandu (Cajanus cajan) sobre o epitélio intestinal e o desempenho de frangos de corte

Um experimento foi conduzido para avaliar a influência de fatores antinutricionais de alguns alimentos sobre o epitélio intestinal e o desempenho de frangos de corte. Foram utilizados 480 pintos de um dia de idade, em um delineamento inteiramente casualizado com quatro tratamentos (A = ração testemunha à base de milho e soja; B = ração contendo Leucaena leucocephala - 6% [LL]; C = ração contendo Leucaena cunningan - 6% [LC] e D = ração contendo feijão guandu - Cajanus cajan - 20% [CC]), quatro repetições e 30 aves por unidade experimental. Houve redução de ganho de peso e aumento da conversão alimentar com a utilização de LL e LC, no período de 1a 21 e de 1a 42 dias de idade. Aos 21 dias de idade, observou-se aumento no tamanho dos vilos do jejuno e na relação vilo:cripta do jejuno e íleo, nas aves que receberam LC. Houve aumento significativo (P<0,05) das células caliciformes no duodeno e jejuno em aves de todos os tratamentos e apenas LL e LC aumentaram as células caliciformes do íleo. Aos 42 dias de idade, houve aumento no número de células caliciformes causado por CC no duodeno e por LL, LC e CC no íleo. O consumo de ração e a profundidade de cripta não foram influenciados. A alteração da morfologia da vilosidade do jejuno, bem como a alteração na relação vilo:cripta no período inicial, influenciou negativamente o ganho de peso e a conversão alimentar nos períodos inicial e total.

Efeito alelopático de extrato aquoso de folhas de Azadirachta indica A. Juss. em alface, soja, milho, feijão e picão-preto

The family Meliaceae has been identified as one of the most promising for biological control, as most species have biologically active compounds. Among the species, the Neem (Azadirachta indica A. Juss) is that stands out most for its efficiency and low toxicity, and it is utilized to control insects, fungi and nematodes. The aim of this work was to verify the allelopathic potential of aqueous extract of fresh leaves of Neem on germination of lettuce, soybeans, maize, beans and Bidens pilosa, as a way to control this weed. The extract was obtained by grinding 200g/L of fresh leaves of the Neem (considered extract 100%) and diluting in distilled water at concentrations of 80, 60, 40, and 20%, and a control with only water. The seeds were kept in the germination chamber at a temperature of 25 degrees C, with photoperiod of 12 hours of light. The aqueous extract affected the germination percentage and average length root of lettuce, with values of 1% and 0.1, cm respectively. For the soybeans, the extract influenced negatively the average time of germination and average speed of germination. There was a drastic reduction of the average root length at higher concentration for soybean, corn and beans. For Bidens pilosa all the parameters analyzed were negatively affected, which means sensitivity to the extract. The bioassay performed in the laboratory showed that the aqueous extract of fresh leaves of Azadirachta indica has allelopathic effect on all species.

Incorporação de mucuna-preta e de restos culturais de milho antes da cultura do feijão (Phaseolus vugaris L.) de inverno

The experiments were conducted during two years (1990/91 and 1992/93), to study the effects of the incorporation of bengal velvetbean and of residues on the development and yield of the winter bean crops. The trial was developed in experimental field at the Faculdade de Engenharia de Ilha Solteira, UNESP, located in Selvíria, MS, Brazil, in dark red latosol covered by savanna vegetation. It was concluded that: the incorporation of residues of corn or corn + bengal velvetbean or only bengal velvetbean had no effects on soil chemical characteristics, evaluated 80 days after the residues incorporation; in yield and bean crop development, no statistical differences were observed among treatments.

Balanço de radiação em cultivos de feijão-vagem com e sem cobertura de polietileno

The radiation budget in agricultural crops is very important on the microclimate characterization, on the water losses determination and on dry matter accumulation of vegetation. This work describes the radiation budget determination in a green beans crop (Phaseolus vulgaris L.), in Botucatu, SP, Brazil (22° 54′S; 48° 27′W; 850 m), under two different conditions: the normal field culture and in a polyethylene greenhouse. The densities of fluxes of radiation were used to construct diurnal curves of the components of global radiation (Rg), reflected radiation (Rr), net radiation (Rn).The arithmetic's relations allowed to obtain the components net short-waves (Rc) and net long-waves (Rl). The analysis of these components related to the leaf area index (LAI) in many phenological phases of the culture showed Rg distributed in 68%, 85%, 17% and 66%, 76%, 10% to Rn, Rc and Rl in the internal and external ambients in a polyethylene greenhouse, respectively.

Biologia comparada de Trialeurodes vaporariorum (West.) (Hemiptera - Homoptera: Aleyrodidae) em soja e cinco cultivares de feijão

With the development of greenhouse grown crops, the whitefly Trialeurodes vaporariorum (West.) has become an important pest. Aiming at verifying the effect of host on the development of this species, the biology of the whitefly was studied on bean (Phaseolus vulgaris L.) cultivars IAC-Carioca Pyatã, IAPAR-57, Jalo Precoce, IAC-Bico de Ouro, IAC-Maravilha and on soybean (Glycine max L.) cultivar Cristalina. The study was conducted at the Laboratório de Entomologia Agrícola - FCA-UNESP, at 25 ± 3°C temperature, 80 ± 10% RH and 14h photophase. The biological parameters assessed were duration, viability and number of nymphal instars; male and female longevity, oviposition period, number of eggs per female and sex ratio; egg viability and incubation period and the life cycle duration. Based on data obtained, fertility life tables were constructed to allow a comparative analysis of the effect of cultivars on the biological development and consequent population growth of this species. The bean cultivars IAC-Maravilha and Jalo Precoce negatively influenced T. vaporariorum development, whereas, the other bean cultivars studied, as well as the soybean cultivar Cristalina, provided good conditions for development of this pest.

Características agronômicas e tecnológicas de genótipos de feijão do grupo comercial Carioca

Cultivars with high yield, adaptability and desirable technological characteristics are a must. The objective of this work was to evaluate agronomic and technologic characters of common bean genotypes from carioca commercial group. Genotypes were cultivated in water growing season, in 2001 and 2002. The experimental design was by randomized blocks with 29 genotypes and four replications. The IAC-Carioca, FT-Bonito, Rudá, Porto Real, CNFC 8008, CNFC 8011, CNFC 8012, CNFC 8013 and CNFC 8156 genotypes yielded above the average. The genotypes IAC-Carioca, CNFC 8012 and CNFC 8156 presented the best results with 3,000 kg ha-1 and 20 minutes cooking time.

Correlação da produtividade do feijão com a resistência à penetração do solo sob plantio direto

The soil mechanical resistance to penetration (PR) has great influence on vegetative development as the root growth and the crop productivity change in inverse proportion. Thus, the objective of this research was to study correlation between the bean grain productivity and the PR in an Oxisol cultivated for four years in no-tillage system at FEIS/UNESP. The attributes PR and yield were determined in a regular grid with 119 sample points. The PR was determined in the layers of 0-0.05, 0.05-0.10, 0.10-0.15, 0.15-0.20 and 0.25-0.30 m. The results were submitted to procedures of descriptive statistics, linear correlation and geostatistic analysis. The linear correlation between the yield and PR was practically null, as in all soil layers investigated it showed determination coefficients (R2) smaller than 0.03 and not significant. The geostatistic analysis showed moderate structure of spatial dependency for PR in the layers of 0.05-0.10 and 0.10-0.15 m, and strong for yield; however, the conjugate spatial analysis of such attributes showed no correlation, therefore, the spatial variability of PR did not influence the yield.

Proteína e qualidade de sementes de feijão (Phaseolus vulgaris L.) em função da adubação nitrogenada em plantio direto

Nitrogen limitation in the common bean (Phaseolus vulgaris L.) produces reduced productivity and seeds with low protein content and physiological quality. We assessed the effects of nitrogen fertilizer side dressing on the protein content and physiological quality of the seeds of P. vulgaris L. cultivars (cv.) IPR Juriti and Pérola grown in plots (soil type = Dystrophic Red Latosol) using a no-tillage system under a thick mulch of millet residues at the Experimental Station of UNESP-Ilha Solteira campus, located in Selvíria, MS (Köppen climate type = Aw) during autumn (March/June) and winter (June/September) 2005. For each cultivar, a randomized block experimental design was used with four replicates and factorially arranged treatments equivalent to 0, 30, 60, 90 and 120 kg.ha-1 added nitrogen (as urea, containing 45% of N) applied as a side dressing during the V4-3 and V4-6 phenological stage corresponding to the 3rd and 6th completely opened trifoliolate leaf on the main stem. Supplementation with up to 120 kg.ha-1 nitrogen promoted a greater increase in crude protein at V4-3 (unsupplemented = 17.6% and 16.3%; 120 kg.ha-1 N = 24.1% and 22.3% for cv. IPR Juriti and Pérola, respectively) than at V4-6 (unsupplemented = 19.2% and 18.3%; 120 kg.ha-1 N = 21.3% and 20.3% for cv. IPR Juriti and Pérola, respectively). About 90% of the crude protein from cv. IPR Juriti was composed of soluble protein compared to 72% for the cv. Pérola. Albumins and globulins represented about 80% of the soluble protein and prolamins were lower at 0.6%. In conclusion, nitrogen fertilization up to 120 kg.ha-1 applied as a side dressing at the V4-3 phenological stage in no-tillage under a thick mulch of millet promoted a greater increase of crude protein in common bean seeds than at the V4-6 stage. The highest accumulation of soluble protein occurred at 90 kg.ha-1 applied nitrogen without having a significant influence on the physiological quality of the seeds.

Sementes de feijão-caupi (Vigna unguiculata L. (Walp), tratadas com extrato de folhas de nim (Azadirachta indica A. Juss.) avaliação da germinação e da incidência de fungos

This study aimed to evaluate the effect of aqueous extract of neem on germination and fungi incidence on seeds of three cultivars (Serrinha, BR 17 and Maranhão) of cowpea. Neem leaves were dryed, crushed and prepared dilutions of 0.5; 1.0; 2.0, 4.0 g dm -3and control. The fungi incidence was evaluated by the test filter paper and germination according to the Rules for Seeds Testing (Regras para Análise de Sementes). In the three cultivars analyzed, reduction in the incidence of Aspergillus sp and Fusarium sp was observed. In relation to the influence of extracts of neem leaves on seed germination, significant effect of extract in Maranhão cultivar was observed, where all concentrations differed from the control, and propovided a considerable increase in the percentage of normal seedlings. It was concluded that the leaf extract of neem was effective in controlling Aspergillus sp, Fusarium sp , Phoma sp and Macrophomina phaseolina at different concentrations in different cultivars and seed germination was stimulated for the Maranhão cultivar.

O modelo 'hydrotime' pode descrever a resposta de sementes de feijão comum (phaseolus vulgaris l.) à temperatura e potenciais de água reduzidos

Germination parameters of the response to temperature and water potential from four common bean (Phaseolus vulgaris) lines based on thermal-time and hydrotime concepts were estimated to verify to what extent they can predict germination under different thermal and water conditions. The cultivars IPR Uirapuru and IAPAR 81 (drought-tolerant), and Grauna and Carioca (not tolerant) were used. The isothermal assays were performed in a temperature gradient block, and the assays with different osmotic potentials (PEG 6000) were performed in germination chambers. Seeds from drought-tolerant cultivars spent less time to germinate at supra-optimum temperatures than non-tolerant ones, and the cultivar Uirapuru (drought-tolerant) germinated faster in response to reduced Ψ and low temperatures. The parameter Ψb(50) did not discriminate between drought-tolerant and non-tolerant lines at the infraoptimum temperature range, but it can be used to identify drought-tolerant lines at high temperatures. In general, the hydrotime model reproduced the actual germination data relatively well, chiefly at higher temperatures. This study evidenced that the hydrotime model can be used to describe the germination of common bean seeds under reduced water potentials, and as a screening tool for drought-tolerant bean genotypes.

Características agronômicas do feijão caupi inoculado em função de lâminas de irrigação e de níveis de nitrogênio

The aim of this study was to evaluate the effect of different irrigation depths and nitrogen levels on the agronomic characteristics of inoculated cowpea. The experiment was arranged in splitplots, with the levels of nitrogen in the plots and the depth of irrigation in the splitplots, in a randomized block design, with two repetitions. The irrigation depth corresponded to 578 mm (L1), 512 mm (L2), 429 mm (L3), 299 mm (L4) and 240 mm (L5), and the nitrogen levels of 0, 30, 60 and 90 kg ha-1 of N. The nitrogen levels showed significant effect on the yield components of cowpea. The irrigation depth showed significant effects on productivity of bean cowpea, with the depth of 426.21 mm estimated for obtaining the maximum productivity of 2820.03 kg ha-1.

Maternal offspring behaviour in curraleiro pé duro naturalized cattle in brazil

The objective of the present study consisted of describing dam and calf suckling behaviour of Curraleiro Pé Duro cattle. In this study, 38 mother-offspring pairs and one mother-offspring-orphan trio were observed for 10 hours daily during three consecutive days spaced every four months over a period of one year. After identification,theanimalswereobserved under field conditions where calf posture and the number of sucklingepisodes(NS),meansucklingduration(MSD),total suckling duration per day (TSD) as well as natural weaning of these animals were recorded. The model assessed the effects of calf sex and age as well as feeding time. Suckling episodes (70.6%) had a duration of one to five minutes and the calf that suckled in the inverted parallel position had greater chances of success during suckling (99.5%); the younger animals had a shorter mean suckling duration (4.0±0.6 minutes) than the older ones (7.5±1.2 minutes) but they showed a higher number of suckling episodes (6.29±1.00 vs. 1.33±0.04 feeds in 10 hours for young and older calves). Only the factor age in the firstthree months was significant for NS,MSD,and TSD;males and females had similar suckling episode length and distribution. While these animals show some traits similar to other cattle breeds such as feeding their calves early in the morning and late in the afternoon, the dams spend large periods of the day away from their calves and suckling is more frequent but for shorter periods of time compared with other breeds. Other unique features such as allo-suckling and formation of day-long crèches are observed in this breed. © 2013 Sociedade Brasileira de Zootecnia;.