Modes of expression and common structural features of the complete phenylalanine ammonia-lyase gene family in parsley.

We describe a complete gene family encoding phenylalanine ammonia-lyase (PAL; EC 4.3.1.5) in one particular plant species. In parsley (Petroselinum crispum), the PAL gene family comprises two closely related members, PAL1 and PAL2, whose TATA-proximal promoter and coding regions are almost identical, and two additional members, PAL3 and PAL4, with less similarity to one another and to the PAL1 and PAL2 genes. Using gene-specific probes derived from the 5' untranslated regions of PAL1/2, PAL3, and PAL4, we determined the respective mRNA levels in parsley leaves and cell cultures treated with UV light or fungal elicitor and in wounded leaves and roots. For comparison, the functionally closely related cinnamate 4-hydroxylase (C4H) and 4-coumarate:CoA ligase (4CL) mRNAs were measured in parallel. The results indicate various degrees of differential responsiveness of PAL4 relative to the other PAL gene family members, in contrast to a high degree of coordination in the overall expression of the PAL, C4H, and 4CL genes. The only significant sequence similarities shared by all four PAL gene promoters are a TATA-proximal set of three putative cis-acting elements (boxes P, A, and L). None of these elements alone, or the promoter region containing all of them together, conferred elicitor or light responsiveness on a reporter gene in transient expression assays. The elements appear to be necessary but not sufficient for elicitor- or light-mediated PAL gene activation, similar to the situation previously reported for 4CL.

The eight hours day for wage earning women: United States Supreme Court upholds the California law : List of eight hours laws

National Consumers' League.

Bill of mortality for ten years at Cambridge (manuscript chart), [1768]

One folded leaf containing a chart in John Winthrop's hand listing the number of deaths by month for 1759-1768 and listing the chief causes of mortality. The verso contains a list of the number of deaths by year according to age groups.

Bill of sale of sloop Cyrus, 1793 February 2

Bill of sale between Thomas Sawyer, Jacob Adams, and Isaac Sawyer, all of Falmouth, Massachusetts, and Ebenezer Storer and William Winthrop for the sloop Cyrus.

Bill of sale of sloop Cyrus, 1795 March 4

Bill of sale transferring William Winthrop's share of the sloop Cyrus to Treasurer Ebenezer Storer. This bill of the sale has a wax seal but is unsigned.

Bill of sale of sloop Cyrus, [1798 March 7 ]

Uncompleted bill of sale for the sloop Cyrus between Ebenezer Storer and buyers Samuel Drinkwater and Abel Sawyer. The document is undated.

Bill of sale of sloop Cyrus, 1795 March 4

Bill of sale transferring William Winthrop's share of the sloop Cyrus to Treasurer Ebenezer Storer. This copy of the sale has William Winthrop's signature.

Bill of lading for shipment of four boxes of books, Oct. 20, 1764

This bill of lading is from Jasper Mauduit's shipment of books (his own gift and those bequeathed by Dr. Avery) to Andrew Oliver. The books were shipped from London to Boston via a ship called Hale Galley, under the direction of Captain Harris Hatch.

Bill of disbursments [for the] sch[oone]r Lydia : manuscript, 1790

List of ship's expenses between Feb. 7 and May 25; dated at Aux Cayes, (Haiti) May 27, 1790; includes money paid to an interpreter at Tobago, workers for ship maintenance, and various harbor officials; and notation of purchases for goods such as sugar, coffee, rice, tar, food, and rum.