Validação de método de análise quantitativa para a triancinolona a partir de complexo ternário por espectrofotometria de UV-Vis

Triamcinolone (TRI), a drug widely used in the treatment of ocular inflammatory diseases, is practically insoluble in water, which limits its use in eye drops. Cyclodextrins (CDs) have been used to increase the solubility or dissolution rate of drugs. The purpose of the present study was to validate a UV-Vis spectrophotometric method for quantitative analysis of TRI in inclusion complexes with beta-cyclodextrin (B-CD) associated with triethanolamine (TEA) (ternary complex). The proposed analytical method was validated with respect to the parameters established by the Brazilian regulatory National Agency of Sanitary Monitoring (ANVISA). The analytical measurements of absorbance were made at 242nm, at room temperature, in a 1-cm path-length cuvette. The precision and accuracy studies were performed at five concentration levels (4, 8, 12, 18 and 20μg.mL -1). The B-CD associated with TEA did not provoke any alteration in the photochemical behavior of TRI. The results for the measured analytical parameters showed the success of the method. The standard curve was linear (r2 > 0.999) in the concentration range from 2 to 24 μg.mL -1. The method achieved good precision levels in the inter-day (relative standard deviation-RSD <3.4%) and reproducibility (RSD <3.8%) tests. The accuracy was about 80% and the pH changes introduced in the robustness study did not reveal any relevant interference at any of the studied concentrations. The experimental results demonstrate a simple, rapid and affordable UV-Vis spectrophotometric method that could be applied to the quantitation of TRI in this ternary complex.

La familia frente a las necesidades de los niños y adolescentes en el desarrollo nacional = The family vis-a-vis the needs of children and adolescents in national development

Incluye Bibliografía

Agregação e viés em medidas de diversidade: uma abordagem computacional intensiva

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

As ticas de matema de cegos sob o viés institucional: da integração à inclusão

Pós-graduação em Educação Matemática - IGCE

Recommended treatment for processed agricultural products vis-a-vis the Agricultural Marketing Protocol (AMP)

Informa sobre recomendaciones surgidas de un Grupo de Trabajo nombrado por el Consejo de Ministros del Mercado Comun de la Comunidad del Caribe, en lo que se refiere a la comercializacion de productos agricolas procesados

Estudos de absorção e emissão de UV/Vis em xerogéis de sílica dopados com cromo obtidos por processo Sol-Gel

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

O humano pelo viés quantitativo: um exame do (neo)positivismo em Speridião Faissol, através da leitura de textos selecionados

Pós-graduação em Geografia - IGCE

Ao mar, navegar é preciso: o pensamento estratégico da Marinha vis-a-vis à política externa brasileira

Pós-graduação em Relações Internacionais (UNESP - UNICAMP - PUC-SP) - FFC

Equidad y protección judicial del derecho a la salud en Colombia

Incluye bibliografía.

Análise do teor de cocaína em amostras apreendidas pela polícia utilizando-se a técnica de cromatografia liquida de alta eficiência com detector UV-Vis

The HPLC technique with UV-Vis detection was employed in the analysis of cocaine content in apprehended samples of cocaine and crack. A peak signal for cocaine was obtained in 3.5 minutes run by using acetonitrile/water (95:5v/v) as a mobile phase. Optimized spectrophotometric signal was obtained at a wavelength of 224 nm. The analytical curve from 1.0 to 40.0 ppm of cocaine was obtained, showing a linear correlation coefficient of 0.9989, with detection and quantification limits of 0.75 ppm and 3.78 ppm, respectively. This methodology was employed at the dosage of confiscated samples of cocaine and crack in the Scientific Police Laboratory of Ribeirão Preto-SP city.

Sociedade de economia mista: possibilidade de recuperação judicial

Pós-graduação em Direito - FCHS

Mitologia dantesca: a referência aos mitos greco-romanos na divina comédia pelo viés da (re)criação poética de Dante Alighieri

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)