Modelling cell lifespan and proliferation: is likelihood to die or to divide independent of age?

In cell lifespan studies the exponential nature of cell survival curves is often interpreted as showing the rate of death is independent of the age of the cells within the population. Here we present an alternative model where cells that die are replaced and the age and lifespan of the population pool is monitored until a, steady state is reached. In our model newly generated individual cells are given a determined lifespan drawn from a number of known distributions including the lognormal, which is frequently found in nature. For lognormal lifespans the analytic steady-state survival curve obtained can be well-fit by a single or double exponential, depending on the mean and standard deviation. Thus, experimental evidence for exponential lifespans of one and/or two populations cannot be taken as definitive evidence for time and age independence of cell survival. A related model for a dividing population in steady state is also developed. We propose that the common adoption of age-independent, constant rates of change in biological modelling may be responsible for significant errors, both of interpretation and of mathematical deduction. We suggest that additional mathematical and experimental methods must be used to resolve the relationship between time and behavioural changes by cells that are predominantly unsynchronized.

Deduced amino acid sequences surrounding the fusion glycoprotein cleavage site and of the carboxyl-terminus of haemagglutinin-neuraminidase protein of the avirulent thermostable vaccine strain I-2 of Newcastle disease virus

A single-tube RT-PCR technique generated a 387 bp or 300 bp cDNA amplicon covering the F-0 cleavage site or the carboxyl (C)-terminus of the HN gene, respectively, of Newcastle disease virus (NDV) strain 1-2. Sequence analysis was used to deduce the amino acid sequences of the cleavage site of F protein and the C-terminus of HN protein, which were then compared with sequences for other NDV strains. The cleavage site of NDV strain 1-2 had a sequence Motif of (112)RKQGRLIG(119), consistent with an avirulent phenotype. Nucleotide sequencing and deduction of amino acids at the C-terminus of HN revealed that strain 1-2 had a 7-amino-acid extension (VEILKDGVREARSSR). This differs from the virulent viruses that caused outbreaks of Newcastle disease in Australia in the 1930s and 1990s, which have HN extensions of 0 and 9 amino acids, respectively. Amino acid sequence analyses of the F and HN genes of strain 1-2 confirmed its avirulent nature and its Australian origin.

A CONTRIBUIÇÃO DO CONCEITO DE SUJEITO EM FRANZ HINKELAMMERT PARA O ESTUDO DA RELIGIÃO

A presente pesquisa tem como objetivo principal demonstrar a contribuição do conceito de sujeito em Franz Hinkelammert para o estudo da religião. Pretende-se mostrar o valor epistemológico crítico desse conceito, compreensível à luz do método dialético transcendental descoberto por Marx e desenvolvido por Hinkelammert, possibilitando sua aplicabilidade no estudo das ciências da religião. Procura-se responder à pergunta posta por Boaventura de Sousa Santos sobre a possibilidade de valorizar o potencial emancipador das subjetividades rebeldes, visando a superação da concepção abstrata de sujeito das ciências empíricas, cuja metodologia científica se fundamenta na objetividade neutral de cunho weberiano. Para tanto, analisa-se a relação entre essa concepção e os sacrifícios humanos dai decorrentes. A invisibilidade ou resignada aceitação desses sacrifícios apontam para a necessidade epistemológica da adoção do conceito de sujeito como critério científico de análise e discernimento, levando à descoberta e crítica das dinâmicas relacionais inconscientes que regem as sociedades entregues à inércia de suas estruturas. Trata-se dum conceito que implica numa teologia subjetiva na qual, Deus se faz presente como cúmplice da resistência das vítimas contra os dominadores , bem como dum critério não religioso que desemboca numa ética autônoma, voltada para uma práxis religiosa humanizadora.

O CONSUMISMO E A DIMENSÃO ESPIRITUAL DAS MARCAS: UMA ANÁLISE CRÍTICA

Esta tese objetiva comprovar que, na sociedade de consumo, indivíduos consumistas transcendem sua relação funcional com as mercadorias, buscando nas marcas de prestígio uma dimensão espiritual, que substitui ou complementa as experiências religiosas tradicionais, e que se revela fetichizada. O consumismo é superlativo de compras, posses e uso, uma dependência de bens não essenciais (supérfluos) para atender aos desejos sem fim. É impossível satisfazer a desejos sem fim: daí a expressão meta transcendental do consumo, posicionada além do alcance e da capacidade de atingi-la. A dimensão transcendente do consumo, por meio do simbolismo das mercadorias potencializado pelas marcas de prestígio, propicia encantamento e sentido ao indivíduo, e se presta a preencher o espaço outrora ocupado pela família, Igreja e comunidade. O sujeito busca, na mercadoria fetichizada pela marca, satisfazer seu desejo mimético, e/ou compensar valores frágeis ou ausentes, o que é reforçado pela propaganda. O sentido último da vida dos indivíduos materialistas produz efeitos imediatos que são positivos para eles e para a economia, mas potencialmente negativos mais à frente para o planeta, para sociedade e para os indivíduos.

ENTRE A COLONIALIDADE E A LIBERTAÇÃO: UMA ANÁLISE DESCOLONIAL DOS DISCURSOS DAS E SOBRE AS CEB S

Esta tese é uma abordagem dos discursos das e sobre as Comunidades Eclesiais de Base com uma análise de recorte descolonial. O objetivo principal é identificar e mostrar a existência de dois tipos de discursos no interior das Ceb s, sendo um dito e outro não-dito, demonstrando a relevância da experiência concreta para a construção de um discurso mais realista e coerente ao modelo de práxis que as Ceb s propõem. Assim, o problema que a tese tem como objeto é que o discurso supostamente único é na verdade um discurso enunciado enquanto outro discurso foi silenciado. Daí que a não percepção dos dois discursos é indício de que um tipo de discurso é apresentado como o enunciado verdadeiro referente às Ceb s. A partir disto, foram propostos como objetivos específicos identificar os temas fundamentais que aparecem nos discursos da base e dos discursos dos assessores, problematizar estes discursos no cotejamento entre si e demonstrar a relevância da diferença de lugares na tipificação do discurso. A tese segue a formulação de três hipóteses. A hipótese principal é que é possível que o discurso enunciado pelos assessores como referente às Ceb s tenha silenciado o outro discurso não-dito, mas vivido pelo povo da base. Em nível secundário, uma hipótese é de que é possível que subjacente ao discurso apresentado como se fosse único, existam diferenças ligadas ao lugar donde falam os atores e que, por sua vez, influenciam o modo como compreendem a história. Outra hipótese secundária é de que é possível que a colonialidade do poder e do saber esteja presente nos discursos sobre as Ceb s, pois a percepção de discursos distintos é já um indício de crítica que não acede ao apresentado de forma hegemônica como sendo único existente. Para isto, a tese está organizada em quatro capítulos: no primeiro é apresentado o discurso dos assessores, com a identificação e problematização dos temas fundamentais emergentes, no segundo é feito o mesmo com o discurso da base; já no terceiro capítulo são apresentados os conceitos que formam o referencial teórico para a análise e no quarto é feita propriamente a análise descolonial dos discursos.

Epiphanien unter eiskaltem Himmel. Zum Prosawerk von Klaus Böldl:for prose work of Klaus Böldl

This essay undertakes the first critical assessment of Klaus Böldl's prose works and discusses the position his three published books hold within the context of contemporary German literature. After an introductory examination of Böldl's contribution to the literary portrayal of the peripheral, in this instance the North European/Scandinavian hemisphere, a contrastive analysis of the plots identifies recurring patterns and motifs. In the main part of the essay, the two focal characteristics of Böldl's writings are explored in depth: the narrative destruction of the threshold between natural history and civilisation, as well as the function of transcendental experiences reminiscent of epiphanies. With reference to theoretical writings by Walter Benjamin and George Steiner these literary epiphanies are interpreted as moments of transcendancy's 'profane presence'. © Springer 2005.

Mathematical modelling and optimal multivariable control of chemical processes

This work reports the developnent of a mathenatical model and distributed, multi variable computer-control for a pilot plant double-effect climbing-film evaporator. A distributed-parameter model of the plant has been developed and the time-domain model transformed into the Laplace domain. The model has been further transformed into an integral domain conforming to an algebraic ring of polynomials, to eliminate the transcendental terms which arise in the Laplace domain due to the distributed nature of the plant model. This has made possible the application of linear control theories to a set of linear-partial differential equations. The models obtained have well tracked the experimental results of the plant. A distributed-computer network has been interfaced with the plant to implement digital controllers in a hierarchical structure. A modern rnultivariable Wiener-Hopf controller has been applled to the plant model. The application has revealed a limitation condition that the plant matrix should be positive-definite along the infinite frequency axis. A new multi variable control theory has emerged fram this study, which avoids the above limitation. The controller has the structure of the modern Wiener-Hopf controller, but with a unique feature enabling a designer to specify the closed-loop poles in advance and to shape the sensitivity matrix as required. In this way, the method treats directly the interaction problems found in the chemical processes with good tracking and regulation performances. Though the ability of the analytical design methods to determine once and for all whether a given set of specifications can be met is one of its chief advantages over the conventional trial-and-error design procedures. However, one disadvantage that offsets to some degree the enormous advantages is the relatively complicated algebra that must be employed in working out all but the simplest problem. Mathematical algorithms and computer software have been developed to treat some of the mathematical operations defined over the integral domain, such as matrix fraction description, spectral factorization, the Bezout identity, and the general manipulation of polynomial matrices. Hence, the design problems of Wiener-Hopf type of controllers and other similar algebraic design methods can be easily solved.

A systems and cybernetic perspective on complex problem solving

This thesis is concerned with Organisational Problem Solving. The work reflects the complexities of organisational problem situations and the eclectic approach that has been necessary to gain an understanding of the processes involved. The thesis is structured into three main parts. Part I describes the author's understanding of problems and suitable approaches. Chapter 2 identifies the Transcendental Realist (TR) view of science (Harre 1970, Bhaskar 1975) as the best general framework for identifying suitable approaches to complex organisational problems. Chapter 3 discusses the relationship between Checkland's methodology (1972) and TR. The need to generate iconic (explanatory) models of the problem situation is identified and the ability of viable system modelling to supplement the modelling stage of the methodology is explored in Chapter 4. Chapter 5 builds further on the methodology to produce an original iconic model of the methodological process. The model characterises the mechanisms of organisational problem situations as well as desirable procedural steps. The Weltanschauungen (W's) or "world views" of key actors is recognised as central to the mechanisms involved. Part II describes the experience which prompted the theoretical investigation. Chapter 6 describes the first year of the project. The success of this stage is attributed to the predominance of a single W. Chapter 7 describes the changes in the organisation which made the remaining phase of the project difficult. These difficulties are attributed to a failure to recognise the importance of differing W's. Part III revisits the theoretical and organisational issues. Chapter 8 identifies a range of techniques embodying W's which are compatible with .the framework of Part I and which might usefully supplement it. Chapter 9 characterises possible W's in the sponsoring organisation. Throughout the work, an attempt 1s made to reflect the process as well as the product of the author's leaving.

Action research in a collective organisation:Developing an education programme in a pressure group

The aim of this project was to develop the education work of an environmental pressure group. The research devised and implemented a project to produce multi-media teaching packs on the urban environment. Whilst this involved understanding environmental education it was necessary to research beyond this to include the various structural and dynamic constraints on change in the field. This presented a number of methodological difficulties; from the resolution of which a model of the research process involved in this project has been developed. It is argued that research oriented towards practical change requires the insights of an experienced practitioner to be combined with the rigours of controlled systematic enquiry. Together these function as a model-building process encompassing intuition, induction and deduction. Model testing is carried out through repeated intervention in the field; thus an interplay between researcher and client ensues such that the project develops in a mutually acceptable direction. In practice, this development will be both unpredictable and erratic. Although the conclusions reached here are based on a single case study they address general methodological issues likely to be encountered in different field settings concerned with different practical problems.

Multispectral fundus analysis

Purpose - To generate a reflectance model of the fundus that allows an accurate non-invasive quantification of blood and pigments. Methods - A Monte Carlo simulation was used to produce a mathematical model of light interaction with the fundus at different wavelengths. The model predictions were compared with fundus images from normal volunteers in several spectral bands (peaks at 507, 525, 552, 585, 596 and 611nm). Th e model was then used to calculate the concentration and distribution of the known absorbing components of the fundus. Results - The shape of the statistical distribution of the image data generally corresponded to that of the model data; the model however appears to overestimate the reflectance of the fundus in the longer wavelength region.As the absorption by xanthophyll has no significant eff ect on light transport above 534nm, its distribution in the fundus was quantified: the wavelengths where both shape and distribution of image and model data matched (<553nm) were used to train a neural network which was then applied to every point in the image data. The xanthophyll distribution thus found was in agreement with published literature data in normal subjects. Conclusion - We have developed a method for optimising multi-spectral imaging of the fundus and a computer image analysis capable of estimating information about the structure and properties of the fundus. Th e technique successfully calculates the distribution of xanthophyll in the fundus of healthy volunteers. Further improvement of the model is required to allow the deduction of other parameters from images; investigations in known pathology models are also necessary to establish if this method is of clinical use in detecting early chroido-retinopathies, hence providing a useful screening and diagnostic tool.

Operating Model of Knowledge Quantum Engineering for Decision-Making in Conditions of Indeterminacy

The operating model of knowledge quantum engineering for identification and prognostic decision- making in conditions of α-indeterminacy is suggested in the article. The synthesized operating model solves three basic tasks: Аt-task to formalize tk-knowledge; Вt-task to recognize (identify) objects according to observed results; Сt-task to extrapolate (prognosticate) the observed results. Operating derivation of identification and prognostic decisions using authentic different-level algorithmic knowledge quantum (using tRAKZ-method) assumes synthesis of authentic knowledge quantum database (BtkZ) using induction operator as a system of implicative laws, and then using deduction operator according to the observed tk-knowledge and BtkZ a derivation of identification or prognostic decisions in a form of new tk-knowledge.

A Two Layered Model for Evolving Web Resources

In this paper the key features of a two-layered model for describing the semantic of dynamical web resources are introduced. In the current Semantic Web proposal [Berners-Lee et al., 2001] web resources are classified into static ontologies which describes the semantic network of their inter-relationships [Kalianpur, 2001][Handschuh & Staab, 2002] and complex constraints described by logical quantified formula [Boley et al., 2001][McGuinnes & van Harmelen, 2004][McGuinnes et al., 2004], the basic idea is that software agents can use techniques of automatic reasoning in order to relate resources and to support sophisticated web application. On the other hand, web resources are also characterized by their dynamical aspects, which are not adequately addressed by current web models. Resources on the web are dynamical since, in the minimal case, they can appear or disappear from the web and their content is upgraded. In addition, resources can traverse different states, which characterized the resource life-cycle, each resource state corresponding to different possible uses of the resource. Finally most resources are timed, i.e. they information they provide make sense only if contextualised with respect to time, and their validity and accuracy is greatly bounded by time. Temporal projection and deduction based on dynamical and time constraints of the resources can be made and exploited by software agents [Hendler, 2001] in order to make previsions about the availability and the state of a resource, for deciding when consulting the resource itself or in order to deliberately induce a resource state change for reaching some agent goal, such as in the automated planning framework [Fikes & Nilsson, 1971][Bacchus & Kabanza,1998].

Multiplierless DCT Algorithm for Image Compression Applications

This paper presents a novel error-free (infinite-precision) architecture for the fast implementation of 8x8 2-D Discrete Cosine Transform. The architecture uses a new algebraic integer encoding of a 1-D radix-8 DCT that allows the separable computation of a 2-D 8x8 DCT without any intermediate number representation conversions. This is a considerable improvement on previously introduced algebraic integer encoding techniques to compute both DCT and IDCT which eliminates the requirements to approximate the transformation matrix ele- ments by obtaining their exact representations and hence mapping the transcendental functions without any errors. Apart from the multiplication-free nature, this new mapping scheme fits to this algorithm, eliminating any computational or quantization errors and resulting short-word-length and high-speed-design.

Representing "Recursive" Default Logic in Modal Logic

The "recursive" definition of Default Logic is shown to be representable in a monotonic Modal Quantificational Logic whose modal laws are stronger than S5. Specifically, it is proven that a set of sentences of First Order Logic is a fixed-point of the "recursive" fixed-point equation of Default Logic with an initial set of axioms and defaults if and only if the meaning of the fixed-point is logically equivalent to a particular modal functor of the meanings of that initial set of sentences and of the sentences in those defaults. This is important because the modal representation allows the use of powerful automatic deduction systems for Modal Logic and because unlike the original "recursive" definition of Default Logic, it is easily generalized to the case where quantified variables may be shared across the scope of the components of the defaults.

Representing Reflective Logic in Modal Logic

The nonmonotonic logic called Reflective Logic is shown to be representable in a monotonic Modal Quantificational Logic whose modal laws are stronger than S5. Specifically, it is proven that a set of sentences of First Order Logic is a fixed-point of the fixed-point equation of Reflective Logic with an initial set of axioms and defaults if and only if the meaning of that set of sentences is logically equivalent to a particular modal functor of the meanings of that initial set of sentences and of the sentences in those defaults. This result is important because the modal representation allows the use of powerful automatic deduction systems for Modal Logic and because unlike the original Reflective Logic, it is easily generalized to the case where quantified variables may be shared across the scope of the components of the defaults thus allowing such defaults to produce quantified consequences. Furthermore, this generalization properly treats such quantifiers since all the laws of First Order Logic hold and since both the Barcan Formula and its converse hold.