Spatial Distribution of Euschistus heros (F.) (Hemiptera: Pentatomidae) in Soybean

Soybean bugs are major crop pests that cause significant reduction in harvest yield and influence grain quality. The aim of this study was to verify the spatial distribution of Euschistus heros (F.) (Hemiptera: Pentatomidae) in conventional and transgenic soybean cultivars. The experiment was conducted during the 2010-2011 crop season in UNESP/FCAV, Jaboticabal, SP, Brazil, in two fields of 10,000-m2 area that were subdivided into 100 plots (10 m × 10 m). The cultivars sown were M 7908 RR and its isoline M-SOY 8001. The number of the first to fifth instars and the number of adults were determined. To evaluate insect dispersion in the area, the following indices were used: variance/mean ratio, Morisita index, Green coefficient, and the k exponent of the negative binomial distribution. To study probabilistic models to describe the spatial distribution of the insects, the adjustments of the Poisson and negative binomial distributions were tested. The first to third instars showed aggregated spatial distribution, whereas the fourth and fifth instars, and adults, isolated or grouped, showed variation in the arrangement, ranging from moderately aggregated to randomly dispersed. During the adjustment of probability distributions, the negative binomial distribution model showed adjustment for the first to third instars, fourth and fifth instars, adults, and fourth and fifth instars plus adults. © 2013 Sociedade Entomológica do Brasil.

Antioxidant activity of basil and oregano extracts added to soybean oil for accelerated storage test

The objective of this research was to evaluate the antioxidant activity of extract of basil and oregano, and its synergistic effect when added to soybean oil and subjected to accelerated storage test. Extracts of basil, oregano and mixtures of both were applied to soybean oil at a concentration of 2.000mg/kg, then the extracts were heated in an oven at 60C for a period of 10 days. Samples were taken every 2 days and analyzed for concentrations of peroxides and conjugated dienes. Synthetic antioxidant tert-butylhydroquinone (TBHQ) at a concentration of 50mg/kg and soybean oil free antioxidant (control) subject to the same conditions were used as benchmarks. The results showed that the antioxidant effect decreases according to oil heating for all treatments. However, the synthetic antioxidant showed superior protection to the soybean oil during the formation of primary oxidation compounds, followed by the natural extracts, which showed no synergism. © 2012 Wiley Periodicals, Inc.

Fatty acids profile and alteration of lemon seeds extract (Citrus limon) added to soybean oil under thermoxidation

This paper aimed at evaluating fatty acids profile and the total alteration of lemon seeds extract added to soybean oil under thermoxidation, verifying the isolated and synergistic effect of these antioxidants. Therefore, Control treatments, LSE (2,400 mg/kg Lemon Seeds Extract), TBHQ (mg/kg), Mixture 1 (LSE + 50 mg/kg TBHQ) and Mixture 2 (LSE + 25 mg/kg TBHQ) were subjected to 180 C for 20 h. Samples were taken at 0, 5, 10, 15 and 20 h intervals and analyzed as for fatty acid profile and total polar compounds. Results were subjected to variance analyses and Tukey tests at a 5% significance level. An increase in the percentage of saturated fatty acids and mono-unsaturated, and decrease in polyunsaturated fatty acids was observed, regardless of the treatments studied. For total polar compounds, it was verified that Mixtures 1 and 2 presented values lower than 25% with 20 h of heating, not surpassing the limits established in many countries for disposal of oils and fats under high temperatures, thus proving the synergistic effect of antioxidants. © 2011 Association of Food Scientists & Technologists (India).

Radiation balance in a soybean ecosystem in the Amazon

O avanço da fronteira agrícola na Amazônia, da forma como vem sendo realizado, tem deixado a comunidade cada vez mais preocupada ante os possíveis impactos ambientais decorrentes desta mudança no uso da terra, devido à grande importância que a Amazônia representa para o clima global. Neste trabalho avaliaram-se os componentes do balanço de radiação à superfície, ao longo do ciclo da soja (Glycine Max (L.) Merryl), em uma área de avanço da fronteira agrícola na Amazônia. Os componentes do balanço de radiação foram monitorados continuamente durante o ciclo da soja, em 2006 e 2007, em uma área de 200 ha de extensão. O monocultivo da soja na Amazônia apresentou uma contínua mudança nos componentes do balanço de radiação, tendo como consequência uma redução na energia disponível para o ambiente devido o aumento na reflexão da superfície. Observou-se uma importante contribuição da radiação solar difusa na interceptação da soja durante dias nublados, mesmo sob condições de dossel incompleto. Por outro lado, após o fechamento do dossel, a interação da soja com a radiação acontece de forma semelhante, independente da condição de nebulosidade.

Simulation of soybean growth and yield under northeastern Amazon climatic conditions

O objetivo deste trabalho foi parametrizar, calibrar e validar uma nova versão do modelo de crescimento e de produtividade da soja desenvolvido por Sinclair, em condições naturais de campo no nordeste da Amazônia. Os dados meteorológicos e os valores de crescimento e de área foliar da soja foram obtidos em um experimento agrometeorológico realizado em Paragominas, PA, de 2006 a 2009. As condições climáticas durante o experimento foram muito distintas, com uma ligeira redução na precipitação em 2007, em virtude do fenômeno El Niño. Houve redução no índice de área foliar (IAF) e na produção de biomassa neste ano, a qual foi reproduzida pelo modelo. A simulação do IAF apresentou raiz do erro quadrado médio (REQM) de 0,55 a 0,82 m2 m‑2, de 2006 a 2009. A simulação da produtividade da soja para os dados independentes apresentou um REQM de 198 kg ha‑1, ou seja, uma superestimativa de 3%. O modelo encontra-se calibrado e validado para as condições climáticas da Amazônia e pode contribuir positivamente para a melhoria das simulações dos impactos da mudança de uso da terra na região amazônica. A versão modificada do modelo de Sinclair simula adequadamente a formação de área foliar, a biomassa total e a produtividade da soja, nas condições climáticas do nordeste da Amazônia.

Alternative methods for detecting soybean seeds genetically modified for resistance to herbicide glyphosate

The objective of this study was to verify application of two methodologies: substrate moistened with herbicide solution (SM) and immersion of seeds in herbicide solution (IH) for detecting soybean seeds genetically modified. For this, non-transgenic and transgenic soybean seeds, harvested in the 2008/2009 crop seasons were used. The treatments with substrate moistened were: SM1) 0.03% herbicide solution, at 25 ºC, with evaluation in the sixth day (hs -0.03% -25 ºC, 6th d); SM2) HS -0.03% -35 ºC, 5th d; SM3) HS -0.03% - 40 ºC, 5th d; and SM4) hs -0.06% -5 ºC, 5th d. In the methodology of immersion of seeds the following treatments were performed: IH1) seed immersion in a 0.6% herbicide solution, at 25 ºC, for 1 h, (si -0.06% -25 ºC, 1 h; IH2) si -0.06% - 35 ºC, 30 min.; IH3) si -0.06% -40 ºC, 30 min.; IH4) si -0.12% -35 ºC, 30 min.; and IH5) si -0.12% -40 ºC, 30 min. Bioassays allow detecting soybean seeds tolerant to glyphosate herbicide within five days. The seeds of non-genetically modified and genetically modified soybean cultivars may be easily distinguished through the treatments SM2 and SM4 of the moistened substrate methodology; and treatments IH3, IH4, and IH5 of seed immersion methodology. Both methodologies are easily feasible, practical, and applicable in seed analysis laboratories, once do not require special equipments.

Discrimination of soybean seed lots by multivariate exploratory techniques

Physiological potential characterization of seed lots is usually performed by germination and vigor tests; however, the choice of a single test does not reflect such potential, once each test assesses seeds of differentiated mode. Multivariate techniques allow understanding structural dependence contained in each variable, as well as characterize groups of seed lots according to specific standards. The study aimed at evaluating variability among soybean seed lots and discriminate these lots by multivariate exploratory techniques as function of seed vigor. Experiment was performed with 20 soybean seed lots (10 lots cv. BRS Valiosa RR and 10 lots cv. M-SOY 7908 RR). Seed physiological potential was assessed by testing for: germination (standard, and under different water availability); vigor (accelerated aging and electrical conductivity); and field seedling emergence. Cluster analysis of seed lots, as well as Principal Component Analysis was performed using data obtained on all tests. Multivariate techniques allowed stratifying seed lots into two distinct groups. Principal Component Analysis showed that values obtained for variables: field seedling emergence, accelerated aging, and germination under different water availability were linked to BRS Valiosa RR; while to variables germination and electrical conductivity, were linked to M-SOY 7908 RR.

Multivariate approach in the selection of superior soybean progeny which carry the RR gene

Efficiency in the use of genetic variability, whether existing or created, increases when properly explored and analysed. Incorporation of biotechnology into breeding programs has been the general practice. The challenge for the researcher is the constant development of new and improved cultivars. The aim of this experiment was to select progenies with superior characteristics, whether or not carriers of the RR gene, derived from bi-parental crosses in the soybean, with the help of multivariate techniques. The experiment was carried out in a family-type experimental design, including controls, during the agricultural year 2010/2011 and 2011/2012 in Jaboticabal in the Brazilian State of São Paulo. From the F3 generation, phenotypically superior plants were selected, which were evaluated for the following traits: number of days to flowering; number of days to maturity; height of first pod insertion; plant height at maturity; lodging; agronomic value; number of branches; number of pods per plant; 100-seed weight; number of seeds per plant; grain yield per plant. Given the results, it appears possible to select superior progeny by principal component analysis. Cluster analysis using the K-means method links progeny according to the most important characteristics in each group and identifies, by the Ward method and by means of a dendrogram, the structure of similarity and divergence between selected progeny. Both methods are effective in aiding progeny selection.

Oviposition preference assessment of Diabrotica speciosa (Coleoptera: Chrysomelidae) for different soybean genotypes

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Feeding preference of Nezara viridula (Hemiptera: Pentatomidae) and attractiveness of soybean genotypes

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Agroclimatic zoning of the state of Minas Gerais for the production of high quality soybean seeds

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Use of EP(Peroxidase) allele in soybean varietal characterization

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Antibiosis in Soybean Genotypes and the Resistance Levels to Spodoptera eridania (Cramer) (Lepidoptera: Noctuidae)

The southern armyworm (SAW) Spodoptera eridania (Cramer) is one of the most common armyworm species defoliating soybeans. Preliminary screening trials have indicated that some soybean genotypes exhibit resistance to SAW. Therefore, in this study, we evaluated the development of SAW larvae fed on ten soybean genotypes in order to identify genotypes with antibiosis-type resistance. Neonate SAW larvae were daily fed with young leaves collected from plants at the vegetative growth stages V4-V5. Larval development and survival were recorded. Genotypes PI 227687 and PI 227682 delayed larval, pupal, and larva-adult development and yielded larvae with the lowest weight and survival and pupae with the lowest weight. Genotypes IAC 100 and DM 339 also negatively affected larval and pupal development and larval survival but at a lower level. Based on our results, the soybean lines PI 227687 and PI 227682 could be used as sources of genes for soybean breeding programs aiming to develop high yield, SAW-resistant cultivars. Moreover, further trials must be carried out under field conditions to validate if the commercial cultivars IAC 100 and DM 339, which expressed moderate levels of antibiosis-type resistance in the laboratory, are effective in suppressing SAW larvae populations.

ASIAN SOYBEAN RUST INTEGRATED MANAGEMENT POTENTIAL: DISEASE SEVERITY, PLANT DEVELOPMENT AND YIELD COMPONENTS, CULTIVAR MG/BR-46 (CONQUISTA)

Soybean rust caused by Phakopsora pachyrhizi Sydow & P. Sydow is one of the major diseases of the soybean crop. The aim of this study was to evaluate the effects of sowing dates, plant populations and reduced doses of fungicides on soybean rust severity and its effects on plant development and yield, cultivar MG/BR46 (Conquista). Field experiments were conducted in the 2009/2010 and 2010/2011 harvests, under natural rust infestation of soybean rust. As from the appearance of the first disease symptoms, also began the fungicide spraying and the disease severity assessments. To understand the nature and extent of the effects of different treatments, a multivariate analysis of factors was applied. For the majority of the agronomic characters and factors, one-third to two-thirds of their variability can be explained by changes in plant populations or by differences in the fungicide treatments, and the remainder, was explained by sowing date variations. The fungicide treatments and sowing dates are determinants in disease severity and its interference on crop productivity. The characters of plant growth are more dependent on plant population variations. Treatments with azoxystrobina + ciproconazol promoted smaller disease severities, reflecting in productivity increase. The plant populations can be reduced up to 160.000 plants ha(-1) without losses in the disease control and the soybean yield. In general, the earliest sowings provided increase in the plant development, although the rust control was less efficient.

INTERCROPPING OF BRAQUIARIA WITH SOYBEAN

The objective of this work was to study the intercropping of Brachiaria brizantha. Marandu with soybeans. The experiment has been planted in a 3 year prevailing area with no-tillage, in eutrophic Oxisol at Maripa - PR. The experimental design was a randomized block with five replications. For the forage study, four treatments were performed which consisted of seeding times brachiaria [early ( seven days before planting soybeans) joint (same day of soybean planting) and after (at stages V-3 and R-1 culture)] intercropping with soybean. To study the yield and crude protein and oil levels of the grain were adopted six treatments, which consisted of sowing dates of capim Marandu [early (seven days before planting soybeans), joint (same day of soybean planting) after (V-3, R-1 and R-5 soybean) and in single culture (single)]. The forager higher productivity of dry matter occurs with early sowing, however, the greatest reduction in soybean yield also occurs in this case. The sowing of Brachiaria until R-5 soy has no influence on the nutritional value of the forage. The intercropped of soybean with Brachiaria brizantha. Marandu has no impact on the leaf Nitrogen content, oil content and enzyme activity of soybean peroxidase. The highest yield of soybeans occurs when it is grown single or Brachiaria is sown in stage R-5. The crude protein content of soybean grain is reduced when brachiaria seeding is early or together with soybeans compared to single culture.