Aspectos clínicos, histopatológicos e moleculares da dermatosparaxia em ovinos White Dorper

Dermatosparaxis in animals is an autosomal recessive disorder of the connective-tissue clinically characterized by skin fragility and hiperextensibility. The disease in White Dorper sheep is caused by mutation (c.421G>T) in the ADAM metalloproteinase with thrombospondin type 1 motif, 2 (ADAMTS2) gene. This study describes the dermatological, histological and the molecular findings of the dermatosparaxis in White Dorper sheep from a herd located in the center-west of São Paulo State. The herd consisted of one ram, four ewe and their lambs. In this herd two lambs had clinical signs consistent with dermatosparaxis. Histopathological evaluation of the affected skin of these two animals also revealed consistent findings with dermatosparaxis, characterized by dysplasia of the collagen, which were arranged in small and fragmented collagen bundles and with foci of degeneration of collagen. Prominent cutaneous appendages and severe hemorrhagic focus in dermis region associated with mild neutrophilic infiltrate in the deep dermis. PCR using DNA blood and specific primers to amplify the mutation region c.421G>T was optimized in order to perform molecular diagnosis of the disease. The direct sequencing of the PCR products proved that the two clinically affected animals had the mutation responsible for dermatosparaxis, previously described for this breed and allowed the definitive diagnosis of the disease. This is the first report of the dermatosparaxis in White Dorper sheep in Brazil and the methodology used to confirm the diagnosis could be used in future studies to assess the prevalence of this mutation in Brazil, allowing the adoption of measures to prevent the spread of this mutation in the Brazilian White Dorper herd.

Consumo e desempenho de ovinos alimentados com silagens de cana-de-açúcar tratadas com óxido de cálcio e cloreto de sódio

The intake and performance of lambs fed with sugarcane silages treated with whitewash and chloride sodium were evaluated. Seven treatments were used: untreated silage (control); silages treated with 0.5; 1.0 and 1.5% of CaO (whitewash); and silages treated with 0.5; 1.0 and 2.0% of NaCl, with four replicates per treatment. The animals were fed a higher amount of dry matter and total digestible nutrients when the sugarcane silages were treated with 0.5% of NaCl, 1.0 and 1.5% of CaO. The intake of neutral detergent fiber and crude protein increased when the lambs were fed silages treated with 0.5% of NaCl and 1.0% of CaO. The average daily gain did not differ among treatments. The inclusion of 0.5% of sodium chloride and 1.0% of whitewash in sugarcane silages increased the intake and improved the performance of lambs.

Potencial de produção de gás metano e dióxido de carbono in vitro dos ingredientes utilizados em dietas para ovinos

This study aimed to evaluate the potential of CH4 and CO2 in vitro production of soybean hulls, sunflower meal, corn, citrus pulp and corn silage. Four rumen-cannulated sheep were fed diets containing the evaluated ingredients at 40:60 forage:concentrate ratio. The gases produced by samples incubation were measured by injection into a gas chromatograph equipped with flame ionization detector. The experimental design was completely randomized with repeated measures, with three replicates for each evaluated food at four different periods. Under the experimental conditions, we verified different potential gas production among the ingredients. The citrus pulp meal was the ingredient with the greatest potential for CO2production. Corn silage and soybean hulls showed the greatest potential while citrus pulp and sunflower meal showed the least potential for CH4 production, when expressed in mL/g of degraded dry matter; therefore, they can be considered, among the evaluated ingredients, those with the lowest environmental impact.

Análise cinemática das articulações dos membros torácicos e pélvicos de ovinos hígidos

Pós-graduação em Ciências Biológicas (Botânica) - IBB

Epidemiologia das infecções por nematódeos gatrintestinais em ovinos criados em Botucatu

Pós-graduação em Medicina Veterinária - FMVZ

Análise cinemática das articulações dos membros torácicos e pélvicos de ovinos hígidos

Pós-graduação em Medicina Veterinária - FMVZ

Diagnóstico morfológico e molecular de Haemonchus spp. em bovinos e ovinos

Pós-graduação em Biologia Geral e Aplicada - IBB

Fermentação, cinética e degradação ruminal em ovinos alimentados com fontes de carboidratos associadas ao óleo

The objective this work was to evaluate the degradation, fermentation and kinetics of passage rumen fluid en sheep fed diets containing different carbohydrate associated with the oil. Four rumen cannulated sheep were allotted in 4 x 4 latin square. The treatments consisted of diets with high neutral detergent soluble fiber and diets with high starch associated or not with 4,2% of oil. Incubation times were 3, 6, 12, 24, 48, 72, 96 and 120 hours and ruminal fluid was collected at 0, 2, 4, 6, 8, 10, 12, and 24 hours after feeding. Diets high neutral detergent fiber soluble resulted in greater degradation of dry matter, crude protein, neutral detergent fiber soluble and starch, and higher pH values, butyric acid production, dilution rate and ruminal recycling compared with diets high starch content. The inclusion of 4,2% oil the different carbohydrate sources had no influence on the fermentation kinetics and degradation of the fiber. High content of neutral detergent soluble fiber in the diet favors the ruminal fermentation compared to the high starch content.

Fauna helmintológica de ovinos provenientes da microrregião de Jaboticabal, estado de São Paulo, Brasil

This study evaluated the prevalence and counting parasitism of different species of helminths of sheep from the micro-region of Jaboticabal of Sao Paulo state. For this, 66 animals naturally infected, four to 36 months of age, raised in pasture, were selected. The results of necropsy revealed the presence of seven genera and 12 species with the following prevalence and mean count: Haemonchus contortus: 100.0% (2947.2); Trichostrongylus colubriformis: 90.9% (3048.8); Cooperia curticei: 56.0% (256.5); Oesophagostomum columbianum: 48.4% (36.0); Cooperia punctata: 30.3% (94.5); Trichostrongylus axei: 22.7% (26.5); Strongyloides papillosus: 19.6% (83.0), Haemonchus contortus (L4): 7.5% (17.2), Cooperia pectinata: 10.6% (12.9), Trichuris ovis: 10.6 % (0.6); Cooperia spatulata 4.5% (0.3); Capillaria bovis: 4.5% (0.1). The mean parasitism of helminthswas 6524.7 per animal. Haemonchus contortus (adults and L4) and Trichostrongylus colubriformis corresponded to 45.4% and 46.7% of the average worm burden totally, respectively. Based in the results obtained in this study, can be concluded that the two most abundant species of helminths and important, the micro-region of Jaboticabal are Trichostrongylus colubriformis and Haemonchus contortus, and these two species amounted to 92.1% of the distribution percentage of helminths collected from all animals. These results demonstrate the importance of conducting a counts of eggs per gram of feces (EPG) in the herds of this region when FAMACHA is used on a particular property, since this method control does not allow to diagnostic the damage/clinical signs in animals infected by T. colubriformis, because this specie does not have hematophagism habit on animals.

Massa de forragem, composição química do capim-massai e comportamento ingestivo de ovinos durante a fase de implantação do eucalipto em sistema silvipastoril

Pós-graduação em Zootecnia - FCAV

Avaliação da absorção colostral em neonatos ovinos da raça Bergamácia

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Efeito da suplementação de cloreto de amônio sobre os equilíbrios eletrolítico e ácido-básico e o pH urinário de ovinos confinados

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

A espectroscopia de reflectância no infravermelho próximo pode predizer consumo, digestibilidade e balanço do nitrogênio de dietas alto concentrado de ovinos

Pós-graduação em Zootecnia - FCAV

Caracterização da resistência à ivermectina de um isolado de Haemonchus contortus em ovinos: se tiver subtitulo

The production of sheep, both in Brazil and worldwide, has shown significant growth in the last years but still has some obstacles to its development, among them are the low indexes caused by parasites, with emphasis on haemoncose. Among the control methods used in the fight against worms are the anthelmintics, and one of the most active principles in this traditional treatment is ivermectin. While being the tool of choice in the treatment of parasitic diseases, improper use of the same led to the emergence of resistant isolates, not only to ivermectin, as several other active principles of antiparasitic drugs

Avaliação das medidas articulares de membros pélvicos em ovinos

The aim of this study was to determine the angle of motion values for hindlimbs in clinically healthy adult Santa Ines sheep using a standard goniometer. Twenty female Santa Ines sheep weighing 32–45 kg (mean30.4, SD=3.7), and aged from three to six years old were used. A standard transparent plastic goniometer was used to measure passive maximum flexion, maximum extension, and range of motion (ROM) of the shoulder, elbow, carpal, hip, stifle, and tarsal joints, in the right and left limbs. The goniometric measurements were done with the sheep awake and in a standing position. The measurements were made in triplicate by two independent investigators. In all evaluated joints there was no significant difference between the means of both sides. No significant difference was observed between measurements performed by the two investigators. The mean (± SD) values of the measurements (degrees) were: 129 ± 4 (ROM) for tarsal joint; 46 ± 4 (flexion), 146 ± 6 (extension), and 100 ± 4 (ROM) for stifle joint; 54 ± 3 (flexion), 143 ± 7 (extension), and 89 ± 5 (ROM) for hip joint. The data obtained is useful to provide objective information of the joints. More studies are necessary using other breeds