Neuropathic Pain Phenotype Does Not Involve the NLRP3 Inflammasome and Its End Product Interleukin-1β in the Mice Spared Nerve Injury Model.

The NACHT, LRR and PYD domains-containing protein 3 (NLRP3) inflammasome is one of the main sources of interleukin-1β (IL-1β) and is involved in several inflammatory-related pathologies. To date, its relationship with pain has not been studied in depth. The aim of our study was to elucidate the role of NLRP3 inflammasome and IL-1β production on neuropathic pain. Results showed that basal pain sensitivity is unaltered in NLRP3-/- mice as well as responses to formalin test. Spared nerve injury (SNI) surgery induced the development of mechanical allodynia and thermal hyperalgesia in a similar way in both genotypes and did not modify mRNA levels of the NLRP3 inflammasome components in the spinal cord. Intrathecal lipopolysaccharide (LPS) injection increases apoptosis-associated speck like protein (ASC), caspase-1 and IL-1β expression in both wildtype and NLRP3-/- mice. Those data suggest that NLRP3 is not involved in neuropathic pain and also that other sources of IL-1β are implicated in neuroinflammatory responses induced by LPS.

The development of decision limits for the GH-2000 detection methodology using additional insulin-like growth factor-I and amino-terminal pro-peptide of type III collagen assays.

The GH-2000 and GH-2004 projects have developed a method for detecting GH misuse based on measuring insulin-like growth factor-I (IGF-I) and the amino-terminal pro-peptide of type III collagen (P-III-NP). The objectives were to analyze more samples from elite athletes to improve the reliability of the decision limit estimates, to evaluate whether the existing decision limits needed revision, and to validate further non-radioisotopic assays for these markers. The study included 998 male and 931 female elite athletes. Blood samples were collected according to World Anti-Doping Agency (WADA) guidelines at various sporting events including the 2011 International Association of Athletics Federations (IAAF) World Athletics Championships in Daegu, South Korea. IGF-I was measured by the Immunotech A15729 IGF-I IRMA, the Immunodiagnostic Systems iSYS IGF-I assay and a recently developed mass spectrometry (LC-MS/MS) method. P-III-NP was measured by the Cisbio RIA-gnost P-III-P, Orion UniQ? PIIINP RIA and Siemens ADVIA Centaur P-III-NP assays. The GH-2000 score decision limits were developed using existing statistical techniques. Decision limits were determined using a specificity of 99.99% and an allowance for uncertainty because of the finite sample size. The revised Immunotech IGF-I - Orion P-III-NP assay combination decision limit did not change significantly following the addition of the new samples. The new decision limits are applied to currently available non-radioisotopic assays to measure IGF-I and P-III-NP in elite athletes, which should allow wider flexibility to implement the GH-2000 marker test for GH misuse while providing some resilience against manufacturer withdrawal or change of assays. Copyright © 2015 John Wiley & Sons, Ltd.

Tutkimus- ja kehitystoiminnan julkinen rahoitus suuryrityksissä

Tässä tutkielmassa on pyritty selvittämään suomalaisten suuryritysten t&k-toiminnan julkista rahoitusta. Aihetta on tarkasteltu monesta eri näkökulmasta: teoriaan, postikyselyvastauksiin ja yksittäisen yrityksen toiminnan kuvaukseen pohjautuen. Tavoitteena on selvittää tällä tavoin kerättyjen tietojen avulla suuryrityksille suunnattuja ulkopuolisen rahoituksen mahdollisuuksia sekä luoda lisävalaistusta siihen, millaista on tehokas julkisen t&k-tuen hakeminen ja käsittely suuryrityksissä. Tutkielma jaoteltiin niin, että ensimmäiseksi tarkastellaan t&k-toimintaa, sen julkista rahoitusta ja tuenantajia. Toisessa osassa käsitellään suuryritysten käytännön kokemuksia t&k-toiminnan julkisrahoitukseen liittyvistä seikoista. Kolmannessa osassa käydään tarkemmin läpi yhden yrityksen t&k-hankkeiden julkista rahoitusta. Tarkastelussa voidaan havaita suuryritysten tukeutuvan julkisen tuen hakemisessa lähinnä Tekesin ja Euroopan unionin teknologiahankkeisiin. Kuitenkin myös muita ulkopuolisen rahoituksen lähteitä oli käytetty. Yritykset pitivät saatua t&k-tukea toimintansa kannalta merkittävänä, vaikka se vaatii yrityksiltä tuenantajasta riippuen enemmän tai vähemmän panostuksia. Monet projektit olisivat jääneet tekemättä ilman tuen saamista. Tutkielman tuloksista voidaan nähdä kansainvälistymisen merkityksen korostuvan yleisesti t&k-toiminnassa. Yrityksen t&k-toiminnan julkisen rahoituksen ollessa tehokasta, on havaittavissa puolestaan yhteys hyvin organisoidun t&k-tuen hakemisen, korkean julkista rahoitusta koskevan tietotason ja tuenantajia koskevan tyytyväisyyden välillä.

Early Recurrence and Cerebral Bleeding in Patients With Acute Ischemic Stroke and Atrial Fibrillation: effect of Anticoagulation and Its Timing: the RAF Study.

BACKGROUND AND PURPOSE: The best time for administering anticoagulation therapy in acute cardioembolic stroke remains unclear. This prospective cohort study of patients with acute stroke and atrial fibrillation, evaluated (1) the risk of recurrent ischemic event and severe bleeding; (2) the risk factors for recurrence and bleeding; and (3) the risks of recurrence and bleeding associated with anticoagulant therapy and its starting time after the acute stroke. METHODS: The primary outcome of this multicenter study was the composite of stroke, transient ischemic attack, symptomatic systemic embolism, symptomatic cerebral bleeding and major extracranial bleeding within 90 days from acute stroke. RESULTS: Of the 1029 patients enrolled, 123 had 128 events (12.6%): 77 (7.6%) ischemic stroke or transient ischemic attack or systemic embolism, 37 (3.6%) symptomatic cerebral bleeding, and 14 (1.4%) major extracranial bleeding. At 90 days, 50% of the patients were either deceased or disabled (modified Rankin score ≥3), and 10.9% were deceased. High CHA2DS2-VASc score, high National Institutes of Health Stroke Scale, large ischemic lesion and type of anticoagulant were predictive factors for primary study outcome. At adjusted Cox regression analysis, initiating anticoagulants 4 to 14 days from stroke onset was associated with a significant reduction in primary study outcome, compared with initiating treatment before 4 or after 14 days: hazard ratio 0.53 (95% confidence interval 0.30-0.93). About 7% of the patients treated with oral anticoagulants alone had an outcome event compared with 16.8% and 12.3% of the patients treated with low molecular weight heparins alone or followed by oral anticoagulants, respectively (P=0.003). CONCLUSIONS: Acute stroke in atrial fibrillation patients is associated with high rates of ischemic recurrence and major bleeding at 90 days. This study has observed that high CHA2DS2-VASc score, high National Institutes of Health Stroke Scale, large ischemic lesions, and type of anticoagulant administered each independently led to a greater risk of recurrence and bleedings. Also, data showed that the best time for initiating anticoagulation treatment for secondary stroke prevention is 4 to 14 days from stroke onset. Moreover, patients treated with oral anticoagulants alone had better outcomes compared with patients treated with low molecular weight heparins alone or before oral anticoagulants.

The specificity of the familial aggregation of early-onset bipolar disorder: A controlled 10-year follow-up study of offspring of parents with mood disorders.

BACKGROUND: Two major sources of heterogeneity of mood disorders that have been demonstrated in clinical, family and genetic studies are the mood disorder subtype (i.e. bipolar (BPD) and major depressive disorder (MDD)) and age of onset of mood episodes. Using a prospective high-risk study design, our aims were to test the specificity of the parent-child transmission of BPD and MDD and to establish the risk of psychopathology in offspring in function of the age of onset of the parental disorder. METHODS: Clinical information was collected on 208 probands (n=81 with BPD, n=64 with MDD, n=63 medical controls) as well as their 202 spouses and 372 children aged 6-17 years at study entry. Parents and children were directly interviewed every 3 years (mean duration of follow-up=10.6 years). Parental age of onset was dichotomized at age 21. RESULTS: Offspring of parents with early onset BPD entailed a higher risk of BPD HR=7.9(1.8-34.6) and substance use disorders HR=5.0(1.1-21.9) than those with later onset and controls. Depressive disorders were not significantly increased in offspring regardless of parental mood disorder subtype or age of onset. LIMITATIONS: Limited sample size, age of onset in probands was obtained retrospectively, age of onset in co-parents was not adequately documented, and a quarter of the children had no direct interview. CONCLUSIONS: Our results provide support for the independence of familial aggregation of BPD from MDD and the heterogeneity of BPD based on patterns of onset. Future studies should further investigate correlates of early versus later onset BPD.

The Intestinal Microbiota Contributes to the Ability of Helminths to Modulate Allergic Inflammation.

Intestinal helminths are potent regulators of their host's immune system and can ameliorate inflammatory diseases such as allergic asthma. In the present study we have assessed whether this anti-inflammatory activity was purely intrinsic to helminths, or whether it also involved crosstalk with the local microbiota. We report that chronic infection with the murine helminth Heligmosomoides polygyrus bakeri (Hpb) altered the intestinal habitat, allowing increased short chain fatty acid (SCFA) production. Transfer of the Hpb-modified microbiota alone was sufficient to mediate protection against allergic asthma. The helminth-induced anti-inflammatory cytokine secretion and regulatory T cell suppressor activity that mediated the protection required the G protein-coupled receptor (GPR)-41. A similar alteration in the metabolic potential of intestinal bacterial communities was observed with diverse parasitic and host species, suggesting that this represents an evolutionary conserved mechanism of host-microbe-helminth interactions.

Disjunct populations of European vascular plant species keep the same climatic niches

Aim Previous research on how climatic niches vary across species ranges has focused on a limited number of species, mostly invasive, and has not, to date, been very conclusive. Here we assess the degree of niche conservatism between distant populations of native alpine plant species that have been separated for thousands of years. Location European Alps and Fennoscandia. Methods Of the studied pool of 888 terrestrial vascular plant species occurring in both the Alps and Fennoscandia, we used two complementary approaches to test and quantify climatic-niche shifts for 31 species having strictly disjunct populations and 358 species having either a contiguous or a patchy distribution with distant populations. First, we used species distribution modelling to test for a region effect on each species' climatic niche. Second, we quantified niche overlap and shifts in niche width (i.e. ecological amplitude) and position (i.e. ecological optimum) within a bi-dimensional climatic space. Results Only one species (3%) of the 31 species with strictly disjunct populations and 58 species (16%) of the 358 species with distant populations showed a region effect on their climatic niche. Niche overlap was higher for species with strictly disjunct populations than for species with distant populations and highest for arctic-alpine species. Climatic niches were, on average, wider and located towards warmer and wetter conditions in the Alps. Main conclusion Climatic niches seem to be generally conserved between populations that are separated between the Alps and Fennoscandia and have probably been so for 10,000-15,000 years. Therefore, the basic assumption of species distribution models that a species' climatic niche is constant in space and time - at least on time scales 104 years or less - seems to be largely valid for arctic-alpine plants.

International survey of acute stroke imaging used to make revascularization treatment decisions.

BACKGROUND: To assess the differences across continental regions in terms of stroke imaging obtained for making acute revascularization therapy decisions, and to identify obstacles to participating in randomized trials involving multimodal imaging. METHODS: STroke Imaging Repository (STIR) and Virtual International Stroke Trials Archive (VISTA)-Imaging circulated an online survey through its website, through the websites of national professional societies from multiple countries as well as through email distribution lists from STIR and the above mentioned societies. RESULTS: We received responses from 223 centers (2 from Africa, 38 from Asia, 10 from Australia, 101 from Europe, 4 from Middle East, 55 from North America, 13 from South America). In combination, the sites surveyed administered acute revascularization therapy to a total of 25,326 acute stroke patients in 2012. Seventy-three percent of these patients received intravenous (i.v.) tissue plasminogen activator (tPA), and 27%, endovascular therapy. Vascular imaging was routinely obtained in 79% (152/193) of sites for endovascular therapy decisions, and also as part of standard IV tPA treatment decisions at 46% (92/198) of sites. Modality, availability and use of acute vascular and perfusion imaging before revascularization varied substantially between geographical areas. The main obstacles to participate in randomized trials involving multimodal imaging included: mainly insufficient research support and staff (50%, 79/158) and infrequent use of multimodal imaging (27%, 43/158) . CONCLUSION: There were significant variations among sites and geographical areas in terms of stroke imaging work-up used tomake decisions both for intravenous and endovascular revascularization. Clinical trials using advanced imaging as a selection tool for acute revascularization therapy should address the need for additional resources and technical support, and take into consideration the lack of routine use of such techniques in trial planning.

Geographic patterns of genetic variation in a broadly distributed marine vertebrate: new insights into loggerhead turtle stock structure from expanded mitochondrial DNA sequences

Previous genetic studies have demonstrated that natal homing shapes the stock structure of marine turtle nesting populations. However, widespread sharing of common haplotypes based on short segments of the mitochondrial control region often limits resolution of the demographic connectivity of populations. Recent studies employing longer control region sequences to resolve haplotype sharing have focused on regional assessments of genetic structure and phylogeography. Here we synthesize available control region sequences for loggerhead turtles from the Mediterranean Sea, Atlantic, and western Indian Ocean basins. These data represent six of the nine globally significant regional management units (RMUs) for the species and include novel sequence data from Brazil, Cape Verde, South Africa and Oman. Genetic tests of differentiation among 42 rookeries represented by short sequences (380 bp haplotypes from 3,486 samples) and 40 rookeries represented by long sequences (~800 bp haplotypes from 3,434 samples) supported the distinction of the six RMUs analyzed as well as recognition of at least 18 demographically independent management units (MUs) with respect to female natal homing. A total of 59 haplotypes were resolved. These haplotypes belonged to two highly divergent global lineages, with haplogroup I represented primarily by CC-A1, CC-A4, and CC-A11 variants and haplogroup II represented by CC-A2 and derived variants. Geographic distribution patterns of haplogroup II haplotypes and the nested position of CC-A11.6 from Oman among the Atlantic haplotypes invoke recent colonization of the Indian Ocean from the Atlantic for both global lineages. The haplotypes we confirmed for western Indian Ocean RMUs allow reinterpretation of previous mixed stock analysis and further suggest that contemporary migratory connectivity between the Indian and Atlantic Oceans occurs on a broader scale than previously hypothesized. This study represents a valuable model for conducting comprehensive international cooperative data management and research in marine ecology.

V-band observations of V4641 Sagittarii

In this note we report high time resolution V Johnson photometry of the microquasar V4641 Sgr (SAX J1819.3-2525 - XTE J1819-254). This source was discovered as an X-ray transient by BeppoSAX and RossiXTE satellites (In't Zand et al. 1999; Markwardt et al. 1999). On 1999 September, a very fast transient optical and X-ray outburst was observed, reaching up to ...

Dynamic interplay between the intermediate filament protein nestin and Cyclin-dependent kinase 5

Cellen har ett s.k. cytoskelett som bl.a. ger stadga åt cellen och deltar i dess form- och rörelsefunktioner. Intermediärfilamenten är en viktig del av cytoskelettet och de har länge varit kända för sina väsentliga roller i att upprätthålla den cellulära organisationen och vävnadernas integritet. På senare år har man insett att intermediärfilamenten har en större funktionell mångsidighet än man tidigare tänkts sig, i och med att en rad olika studier har visat betydelsen av intermediärfilamenten vid olika signaleringprocesser. Dessa proteinnätverk samverkar nämligen med kinaser och andra viktiga signalfaktorer och deltar därmed i cellens signaleringmaskineri. Intermediärfilamentproteinet nestin används ofta som en markör för stamceller men dess fysiologiska funktioner är i stort sett okända. Interaktion mellan nestin och ett signalkomplex bestående av cyklin-beroende kinas 5 (eng. Cyclin-dependent kinase, Cdk5) och dess aktivatorprotein p35 upptäcktes i vårt laboratorium före denna avhandling påbörjades. Därför var syftet med min avhandling att undersöka den funktionella betydelsen av nestin i regleringen av Cdk5/p35 komplexet. Cdk5 är ett multifunktionellt kinas som reglerar både utvecklingen och stressreaktioner i nerver och muskler. Vi visade att nestin skyddar neuronala stamceller under oxidativ stress genom dess förmåga att hämma Cdk5s skadliga aktivitet. Genom att förankra Cdk5/p35 komplexet, reglerar nestin den subcellulära lokaliseringen av Cdk5/p35 och minskar klyvningen av p35 till den mer stabila aktivatorn p25. Vi demonstrerade också aktiveringsmekanismen för Cdk5 under differentiering av muskelceller. Proteinkinas C zeta (PKCzeta) avslöjades ha en förmåga att accelera klyvningen av p35 till p25, och därmed öka aktiviteten hos Cdk5. Nestin kunde genom sin förmåga att reglera Cdk5 signalkomplexet styra muskelcellernas differentiering. Denna doktorsavhandling har på ett avgörande vis ökat förståelsen av de reglerande mekanismer som styr Cdk5 aktivering. Avhandling presenterar nestin och PKCzeta som kritiska faktorer i denna reglering. Vidare innehåller avhandlingen ny information om de cellulära funktionerna hos nestin som vi har visat vara en viktig reglerare av cellernas överlevnad och differentiering.

I eld, i blod, i frost, i svält : möten med veteraners, lottors och sjuksköterskors berättande om krig

Folkligt berättande om krig är i fokus i min avhandling i folkloristik som baserar sig på intervjuer med tio män och tio kvinnor som själva deltagit aktivt i krigen 1939–1944 i Finland. Intervjuerna utgick från deras upplevelser av J.L. Runebergs verk Fänrik Ståls sägner och övergick sedan i berättande om deras egna erfarenheter från vinterkriget och fortsättningskriget. Jag arbetar med oral history som teoretiskt ramverk, och har ett antagande om att man genom studiet av personligt berättande om historiska händelser kan få syn på annat än sådant som förs fram av historieskrivningen. Fyra innehållsliga huvudteman har utkristalliserat sig för mig i analysen av materialet: möten med tjänstgöringen, möten med kroppen, möten med olika etniska andra, och möten med tiden efter kriget. Utöver den tematiska analysen studerar jag också hur jag som forskare är med och skapar materialet under intervjuerna, och jag ser bland annat att jag som hör till intervjupersonernas barnbarnsgeneration fick höra hur de på flera plan vill undervisa mig dels om krigshändelserna, dels om hur man borde förhålla sig till kriget och Finland. Som ett led i detta använder sig intervjupersonerna av något som jag kallar för typfigurer. Genom återkommande typfigurer som exempelvis ”Den ståndaktige soldaten”, ”Den elaka styvmodern” eller ”Kommunisten” förmedlas en viss innehållslig kunskap om krig samtidigt som de genererar vissa folkliga moraluppfattningar. I min avhandling visar jag att berättandet om krigen 1939–1944 är symboliskt laddat och att det mycket starkt förmedlar värderingar om vad som är rätt och riktigt. Man kan säga att berättande om krig är mytologiserande, och att det mytologiserar så väl kriget i sig som landet Finland, och härmed kan sägas producera sanningar som inte kan ifrågasättas. Genren krigsberättelser är inte det samma som berättande om krig. En konsekvens av detta är ett könsbestämt och ofta omedvetet nedtystande av kvinnor och ett ointresse för deras upplevelser och erfarenheter. Detta visar på etablerade och könsbestämda definitioner av vad som ses som historia och vilka roller som får figurera i den.

Hälsans grund kan bara hjärtat förstå : ett sökande efter kunskap om hälsa i ljuset av Blaise Pascals tänkande

The aim of this thesis is to gain new understanding of health based on the work Penseés (Thoughts) by Blaise Pascal. The research interest originates from the motive of the heart for the greatness of man in conjunction with his baseness. The study is hermeneutic and the purpose is to describe health formulated as new abductive theses based on the assumption that caring science and caring theology can be conjoined in the same ontology. The work Penseés is, in Gadamer’s sense, alive since the text is constantly being made use of and is in this occurence interpreted through the requirements made by caring science. Pascal’s arguments continue through the historically effected consciousness while the interpretation is confronted with the ontological model of health. In the initial horizon we find the understanding of the motion of health and the extension towards text materials which is undertaken by Pascal’s Penseés. to open up the perspective and be able to project the horizon of the text. In the encounter with the text, the core content will first be discussed in an interpretative part after which the understanding gained will be summarized into new abductive assumptions. On the basis of Gadamer’s emphasis on the applications of the interpretations, the merging of the horizons will identify health as something desired by man, what he is and what he wants to accomplish. The creation of health is the reason of the heart, and on the basis of what man wants to confide in, the rise of health will be established. The totality of greatness and baseness of man is achieved by the accomplishment of health. That man owns spiritual devotedness means health whereas unhealthy means that he is insecure of his character and leads a life with ill-considered amusements. When according to Pascal, man’s desires and thoughts are joined in the heart, a connecting relation will arise. Hence the spiritual edification that man will fail to secure if he puts too much of his confidence in transient reason is included, something that is insufficient when it comes to understand the meaning of health.

Savnets tone i ensomhetens melodi : ensomhet hos aleneboende personer med alvorlig psykisk lidelse

Studiens overordnede hensikt er å bestemme begrepet ensomhet i et vårdvitenskapelig perspektiv. Avhandlingen er en monografisk eksplorativ hermeneutisk studie og oppbygget i fire større helheter, der første hoveddel starter med en inntreden i den hermeneutiske spiral, og hvor paradigme, ontologiske, epistemologiske og metodologiske overveielser angir studiens videre retning. Andre hoveddel anses som studiens teoretiske substans og belyser ensomhetens `Hva´ gjennom ulike teoretiske perspektiv og begrepsanalyse av ensom. I belysningen framkommer ensomhet både som lidelse og som meningsfullt for eksistensen. Første hoveddel avsluttes med en bestemmelse av ensomhet som substanskunnskap innenfor vårdvitenskapens helse-og lidelsesforhold som utgjør ulike nivåer av gjørende, værende og vardende. Tredje hoveddel er en empiriskstudie med deltagende observasjon, forskersamtaler og kvalitative intervjuer i samtaleform. Informantene var åtte aleneboende personer med alvorlig psykisk lidelse i en storby og foregikk over ca. ett år. I tolkningsprosessen søkes en gradvis fordypende forståelse av den enkelte informants ensomhet i relasjon til vårdvitenskapens livs-, sykdoms- og vårdlidelse. Tolkningsrammen er i det vesentligefra første teoridel, samtidig som nye tolkningsteoretikere innlemmes som opplysende "lamper". Forståelsens tolkningsresultater framkommer gjennom en sammenfattende diskusjon, der livsberettelsens felles skjebne synliggjør savn som ideografisk invarians. Fjerde hoveddel er en vårdvitenskapelig filosofisk belysning av forholdet ensomhet og savn, hvor Levinas' fundamentaletikk utgjør tolkningsrammen. Studien avsluttes med en horisontsammensmeltning av forholdet ensomhet og savngjennom fellesskap og oppfyllelse i tid og rom. Horisontsammensmeltningen viserat ensomhet og savn i sin dybdestruktur forholder seg til hverandre i en dialektikk til motsetningene: fellesskap og oppfyllelse. Det nedfelles fem teser som ansatser til vårdvitenskapelig teori. Resultatene får implikasjoner for så vel pedagogikk som vårdpraksis.

Formaterade nyheter : studier i hur tv-nyheter formatmässigt editeras, gestaltas och tas emot

Under de trettio senaste åren har tv-nyhetsjournalistiken som genre, d.v.s. som en egen särpräglad läsart och form av journalistik, på olika sätt förändrats. Hur denna formatförändring ser ut och om tv-nyheter i takt med samhällets och massmediernas allmänna kommersialisering har blivit mer tabloida är två centrala frågeställningar i avhandlingen. Nyhetsprogrammet TV-nytt i public service-företaget Yle:s svenskspråkiga kanal FST5, har långt en monopolställning. På svenska har TV-nytt inga konkurrenter i tv-etern i Finland och som ett sorts flaggskepp för hela FST5, har nyhetsredaktionen länge haft en stark ställning och uppbackning på svenska Yle. Så om t.o.m. TV-nytts nyhetsformat har förändrats och t.ex. blivit mer tabloida, torde åtminstone public service-nyheter i tv överlag ha förändrats minst lika mycket. Detta granskas empiriskt i avhandlingen. Utan att i frågeställningarna även ta med hur mottagarna, det vill säga hur tv-tittarna reagerar på tv-nyheter, är det svårt att mer ingående och trovärdigt förklara tv-nyheters roll i samhället. Därför är det viktigt att även fråga hur tittarnas intresse och uppmärksamhet påverkas av olika sorts tv-nyheter och hur deras ihågkomst samt nyhetsförståelse påverkas av olika slags tv-nyhetsformat såsom ett mer tabloidiserat dylikt? Avhandling har följaktligen fyra syften: att undersöka vad tv-nyhetsformatet för public service-tv består av, hur detta format ur olika synvinklar har förändrats, om det har tabloidiserats och hur tittarna reagerar på olika tv-nyhetsformat. Avhandlingen består av tre innehållsanalyser och ett laboratorietest. Resultaten visar i korthet att tv-nyhetsformatet både på inslags- och sändningsnivå kan sägas ha två olika formatdimensioner; det audiovisuellt editerade och det journalistiskt innehållsgestaltande formatet. Det senare formatet har för TV-nytts del sedan 1980-talet förändrats mycket mindre än det förra och i synnerhet det audiovisuellt editerade formatet har tydligt utvecklats i en tabloid riktning. Resultaten från de laborativa publiktesterna visar igen att tittarnas uppmärksamhet och intresse i första hand har med audiovisuell editering och allmän visualitet att göra. Mer tabloida nyhetsinslag får därför generellt mer uppmärksamhet än övriga inslag. I synnerhet bildtempo, bildsättning och inslagslängd är här utslagsgivande. Det att tabloidisering ger förbättrad uppmärksamhet betyder ändå inte att tittarna mycket bättre kommer ihåg eller bättre förstår tabloida nyhetsinslag än icke-tabloida. Testerna visar samtidigt att även längre, icke-tabloida nyhetsinslag väl kan fånga tittarens uppmärksamhet och fasta i minnet om inslaget är i en sorts ideologisk resonans med tittaren.