The postnatal development of spinal sensory processing

The mechanisms by which infants and children process pain should be viewed within the context of a developing sensory nervous system. The study of the neurophysiological properties and connectivity of sensory neurons in the developing spinal cord dorsal horn of the intact postnatal rat has shed light on the way in which the newborn central nervous system analyzes cutaneous innocuous and noxious stimuli. The receptive field properties and evoked activity of newborn dorsal horn cells to single repetitive and persistent innocuous and noxious inputs are developmentally regulated and reflect the maturation of excitatory transmission within the spinal cord. These changes will have an important influence on pain processing in the postnatal period.

DNA repeats identify novel virulence genes in Haemophilus influenzae.

The whole genome sequence (1.83 Mbp) of Haemophilus influenzae strain Rd was searched to identify tandem oligonucleotide repeat sequences. Loss or gain of one or more nucleotide repeats through a recombination-independent slippage mechanism is known to mediate phase variation of surface molecules of pathogenic bacteria, including H. influenzae. This facilitates evasion of host defenses and adaptation to the varying microenvironments of the host. We reasoned that iterative nucleotides could identify novel genes relevant to microbe-host interactions. Our search of the Rd genome sequence identified 9 novel loci with multiple (range 6-36, mean 22) tandem tetranucleotide repeats. All were found to be located within putative open reading frames and included homologues of hemoglobin-binding proteins of Neisseria, a glycosyltransferase (IgtC gene product) of Neisseria, and an adhesin of Yersinia. These tetranucleotide repeat sequences were also shown to be present in two other epidemiologically different H. influenzae type b strains, although the number and distribution of repeats was different. Further characterization of the IgtC gene showed that it was involved in phenotypic switching of a lipopolysaccharide epitope and that this variable expression was associated with changes in the number of tetranucleotide repeats. Mutation of IgtC resulted in attenuated virulence of H. influenzae in an infant rat model of invasive infection. These data indicate the rapidity, economy, and completeness with which whole genome sequences can be used to investigate the biology of pathogenic bacteria.

The hippocampal formation participates in novel picture encoding: evidence from functional magnetic resonance imaging.

Considerable evidence exists to support the hypothesis that the hippocampus and related medial temporal lobe structures are crucial for the encoding and storage of information in long-term memory. Few human imaging studies, however, have successfully shown signal intensity changes in these areas during encoding or retrieval. Using functional magnetic resonance imaging (fMRI), we studied normal human subjects while they performed a novel picture encoding task. High-speed echo-planar imaging techniques evaluated fMRI signal changes throughout the brain. During the encoding of novel pictures, statistically significant increases in fMRI signal were observed bilaterally in the posterior hippocampal formation and parahippocampal gyrus and in the lingual and fusiform gyri. To our knowledge, this experiment is the first fMRI study to show robust signal changes in the human hippocampal region. It also provides evidence that the encoding of novel, complex pictures depends upon an interaction between ventral cortical regions, specialized for object vision, and the hippocampal formation and parahippocampal gyrus, specialized for long-term memory.

Calcium-dependent oligonucleotide antagonists specific for L-selectin.

The selectins are calcium-dependent C-type lectins that recognize complex anionic carbohydrate ligands, initiating many cell-cell interactions in the vascular system. Selectin blockade shows therapeutic promise in a variety of inflammatory and postischemic pathologies. However, the available oligosaccharide ligand mimetics have low affinities and show cross-reaction among the three selectins, precluding efficient and specific blockade. The SELEX (systematic evolution of ligands by exponential enrichment) process uses combinatorial chemistry and in vitro selection to yield high affinity oligonucleotides with unexpected binding specificities. Nuclease-stabilized randomized oligonucleotides subjected to SELEX against recombinant L-selectin yielded calcium-dependent antagonists with approximately 10(5) higher affinity than the conventional oligosaccharide ligand sialyl LewisX. Most of the isolated ligands shared a common consensus sequence. Unlike sialyl LewisX, these antagonists show little binding to E- or P-selectin. Moreover, they show calcium-dependent binding to native L-selectin on peripheral blood lymphocytes and block L-selectin-dependent interactions with the natural ligands on high endothelial venules.

Subparsec-scale structure and evolution of Centaurus A (NGC5128).

We present a series of 8.4-GHz very-long-baseline radio interferometry images of the nucleus of Centaurus A (NGC5128) made with a Southern Hemisphere array, representing a 3.3-year monitoring effort. The nuclear radio jet is approximately 50 milliarcseconds in extent, or at the 3.5-megaparsec distance of NGC5128, approximately 1 parsec in length. Subluminal motion is seen and structural changes are observed on time scales shorter than 4 months. High-resolution observations at 4.8 and 8.4 GHz made in November 1992 reveal a complex morphology and allow us to unambiguously identify the self-absorbed core located at the southwestern end of the jet.

Efficient gene transfer into human hepatocytes by baculovirus vectors.

Viral vectors are the most efficient tools for gene delivery, and the search for tissue-specific infecting viruses is important for the development of in vivo gene therapy strategies. The baculovirus Autographa californica nuclear polyhedrosis virus is widely used as a vector for expression of foreign genes in insect cells, and its host specificity is supposed to be restricted to arthropods. Here we demonstrate that recombinant A. californica nuclear polyhedrosis virus is efficiently taken up by human hepatocytes via an endosomal pathway. High-level reporter gene expression from heterologous promoters was observed in human and rabbit hepatocytes in vitro. Mouse hepatocytes and some other epithelial cell types are targeted at a considerably lower rate. The efficiency of gene transfer by baculovirus considerably exceeds that obtained by calcium phosphate or lipid transfection. These properties of baculovirus suggest a use for it as a vector for liver-directed gene transfer but highlight a potential risk in handling certain recombinant baculoviruses.

Memorias de licenciatura: resúmenes de las memorias de licenciatura leídas en el Departamento de Historia Contemporánea de la Universidad de Alicante

Resúmenes de las memorias: GUTIERREZ LLORET, Rosa Ana: La I República. El comportamiento político de la burguesía alicantina. Leída el 30 de noviembre de 1983. Director: Dr. D. Salvador Forner Muñoz; FERRANDEZ ALBERCA, Patrocinio: Fuentes para la historia electoral de la provincia de Alicante. La sección de Elecciones y Censo Electoral del Archivo de la Diputación Provincial de Alicante. Leída el 24 de enero de 1984. Director: Dr. Salvador Forner Muñoz; MARTINEZ MICO, M.ª Angeles: Documentación de las secciones de Contabilidad, Fomento, Quintas y Suministros del Archivo de la Diputación Provincial de Alicante. Leída el 23 de marzo de 1984. Director: Doctor Salvador Forner Muñoz; GONZALVEZ ALONSO, José Vicente: Elche 1854-1868. Demografía, Sociedad y Régimen Municipal. Leída el17 de mayo de 1984. Director: Dr. Glicerio Sánchez Recio; AMOROS SANTO, Luis: Documentación de las secciones de «Cuentas de Propios, Arbitrios y Presupuestos Municipales» y «Gobierno Civil», del Archivo de la Diputación Provincial de Alicante. Leída el 18 de mayo de 1984. Director: Dr. Salvador Forner Muñoz; DE GABRIEL POVEDA, Francisca: Fondos de la sección de Beneficencia del Archivo de la Diputación Provincial de Alicante. Leída el 18 de junio de 1984. Director: Dr. Salvador Forner Muñoz; HERNANDEZ FERRIS, Rafael: La sociedad cooperativa «El trabajo», 1880-1942. Leída el 4 de octubre de 1984. Director: Dr. Glicerio Sánchez Recio; SANTACREU SOLER, José Miguel: Moneda y municipios en la provincia de Alicante durante la guerra civil española (la crisis monetaria de 1937). Leída el 6 de noviembre de 1984. Director: Dr. Salvador Forner Muñoz.

Letter from Dr. Nathaniel Lardner to Jasper Mauduit listing books donated to Harvard, July 12, 1764, and manuscript copy of the letter

A pencilled note in the hand of John Langdon Sibley reads: "Probably the handwriting of Dr. Lardner." Sibley based his attribution on a letter from Nathaniel Lardner to Edward Wigglesworth, also written July 12, 1764, in the Houghton Library's autograph file during the nineteenth century. Verso of letter contains list, in another hand, of books donated to Harvard by Joseph Jennings.

A manual of the mosses of western Pennsylvania and adjacent regions.

no.6

Kolkata, India, City Planning, 1913 (Raster Image)

This layer is a georeferenced raster image of the historic paper map entitled: City of Calcutta, E. P. Richards. It was printed by Jennings & Bewley in 1913. Scale 1:21,120. Covers Kolkata and a portion of Hāora, India. The image inside the map neatline is georeferenced to the surface of the earth and fit to the 'WGS 1984 UTM Zone 45N' coordinate system. All map collar and inset information is also available as part of the raster image, including any inset maps, profiles, statistical tables, directories, text, illustrations, index maps, legends, or other information associated with the principal map. This map shows features such as residential and industrial zoning districts and redevelopment zones, roads, railroads, drainage, canals, built-up areas and selected buildings, fortification, docks, parks, and more. Includes index, note, legend of class dwellings, and census "map" (i.e., chart). This layer is part of a selection of digitally scanned and georeferenced historic maps from The Harvard Map Collection as part of the Imaging the Urban Environment project. Maps selected for this project represent major urban areas and cities of the world, at various time periods. These maps typically portray both natural and manmade features at a large scale. The selection represents a range of regions, originators, ground condition dates, scales, and purposes.