Effects and uptake of gold nanoparticles deposited at the air–liquid interface of a human epithelial airway model

The impact of nanoparticles (NPs) in medicine and biology has increased rapidly in recent years. Gold NPs have advantageous properties such as chemical stability, high electron density and affinity to biomolecules, making them very promising candidates as drug carriers and diagnostic tools. However, diverse studies on the toxicity of gold NPs have reported contradictory results. To address this issue, a triple cell co-culture model simulating the alveolar lung epithelium was used and exposed at the air-liquid interface. The cell cultures were exposed to characterized aerosols with 15 nm gold particles (61 ng Au/cm2 and 561 ng Au/cm2 deposition) and incubated for 4 h and 24 h. Experiments were repeated six times. The mRNA induction of pro-inflammatory (TNFalpha, IL-8, iNOS) and oxidative stress markers (HO-1, SOD2) was measured, as well as protein induction of pro- and anti-inflammatory cytokines (IL-1, IL-2, IL-4, IL-6, IL-8, IL-10, GM-CSF, TNFalpha, INFgamma). A pre-stimulation with lipopolysaccharide (LPS) was performed to further study the effects of particles under inflammatory conditions. Particle deposition and particle uptake by cells were analyzed by transmission electron microscopy and design-based stereology. A homogeneous deposition was revealed, and particles were found to enter all cell types. No mRNA induction due to particles was observed for all markers. The cell culture system was sensitive to LPS but gold particles did not cause any synergistic or suppressive effects. With this experimental setup, reflecting the physiological conditions more precisely, no adverse effects from gold NPs were observed. However, chronic studies under in vivo conditions are needed to entirely exclude adverse effects.

The recoil transfer chamber - An interface to connect the physical preseparator TASCA with chemistry and counting setups

Performing experiments with transactinide elements demands highly sensitive detection methods due to the extremely low production rates (one-atom-at-a-time conditions). Preseparation with a physical recoil separator is a powerful method to significantly reduce the background in experiments with sufficiently long-lived isotopes (t1/2≥0.5 s). In the last years, the new gas-filled TransActinide Separator and Chemistry Apparatus (TASCA) was installed and successfully commissioned at GSI. Here, we report on the design and performance of a Recoil Transfer Chamber (RTC) for TASCA—an interface to connect various chemistry and counting setups with the separator. Nuclear reaction products recoiling out of the target are separated according to their magnetic rigidity within TASCA, and the wanted products are guided to the focal plane of TASCA. In the focal plane, they pass a thin Mylar window that separates the ∼1 mbar atmosphere in TASCA from the RTC kept at ∼1 bar. The ions are stopped in the RTC and transported by a continuous gas flow from the RTC to the ancillary setup. In this paper, we report on measurements of the transportation yields under various conditions and on the first chemistry experiments at TASCA—an electrochemistry experiment with osmium and an ion exchange experiment with the transactinide element rutherfordium.

Design and implementation of a 3D computer game controller using inertial MEMS sensors

Though 3D computer graphics has seen tremendous advancement in the past two decades, most available mechanisms for computer interaction in 3D are high cost and targeted for industry and virtual reality applications. Recent advances in Micro-Electro-Mechanical-System (MEMS) devices have brought forth a variety of new low-cost, low-power, miniature sensors with high accuracy, which are well suited for hand-held devices. In this work a novel design for a 3D computer game controller using inertial sensors is proposed, and a prototype device based on this design is implemented. The design incorporates MEMS accelerometers and gyroscopes from Analog Devices to measure the three components of the acceleration and angular velocity. From these sensor readings, the position and orientation of the hand-held compartment can be calculated using numerical methods. The implemented prototype is utilizes a USB 2.0 compliant interface for power and communication with the host system. A Microchip dsPIC microcontroller is used in the design. This microcontroller integrates the analog to digital converters, the program memory flash, as well as the core processor, on a single integrated circuit. A PC running Microsoft Windows operating system is used as the host machine. Prototype firmware for the microcontroller is developed and tested to establish the communication between the design and the host, and perform the data acquisition and initial filtering of the sensor data. A PC front-end application with a graphical interface is developed to communicate with the device, and allow real-time visualization of the acquired data.

DESIGN AND IMPLEMENT DYNAMIC PROGRAMMING BASED DISCRETE POWER LEVEL SMART HOME SCHEDULING USING FPGA

With the development and capabilities of the Smart Home system, people today are entering an era in which household appliances are no longer just controlled by people, but also operated by a Smart System. This results in a more efficient, convenient, comfortable, and environmentally friendly living environment. A critical part of the Smart Home system is Home Automation, which means that there is a Micro-Controller Unit (MCU) to control all the household appliances and schedule their operating times. This reduces electricity bills by shifting amounts of power consumption from the on-peak hour consumption to the off-peak hour consumption, in terms of different “hour price”. In this paper, we propose an algorithm for scheduling multi-user power consumption and implement it on an FPGA board, using it as the MCU. This algorithm for discrete power level tasks scheduling is based on dynamic programming, which could find a scheduling solution close to the optimal one. We chose FPGA as our system’s controller because FPGA has low complexity, parallel processing capability, a large amount of I/O interface for further development and is programmable on both software and hardware. In conclusion, it costs little time running on FPGA board and the solution obtained is good enough for the consumers.

The Design of Networked Exertion Games

Incorporating physical activity and exertion into pervasive gaming applications can provide health and social benefits. Prior research has resulted in several prototypes of pervasive games that encourage exertion as interaction form; however, no detailed critical account of the various approaches exists. We focus on networked exertion games and detail some of our work while identifying the remaining issues towards providing a coherent framework. We outline common lessons learned and use them as the basis for generalizations for the design of networked exertion games. We propose possible directions of further investigation, hoping to provide guidance for future work to facilitate greater awareness and exposure of exertion games and their benefits.

Vitreoretinal Interface Changes in Geographic Atrophy.

PURPOSE Geographic atrophy (GA) is the end-stage manifestation of atrophic age-related macular degeneration (AMD). The disease progresses slowly over time, eventually causing loss of central vision. Its cause and pathomechanism are not fully known. Previous studies have suggested that vitreoretinal traction (VRT) may contribute to the progression of neovascular AMD. The aim of this study was to examine whether an association between changes at the vitreoretinal interface (VRI), in particular traction (VRT), and the characteristics and progression of GA in eyes with dry AMD can be established. DESIGN Clinic-based prospective cohort study. PARTICIPANTS A total of 97 patients (age range, 61-90 years; mean, 78.4 years) with GA secondary to dry AMD were enrolled. Patients exhibiting neovascular signs on fluorescein angiography in either eye were excluded. METHODS The VRI changes were examined using spectral-domain optical coherence tomography (SD-OCT). Characteristics of GA were examined using fundus autofluorescence (FAF) imaging. All imaging was performed using a Spectralis SLO+OCT device (Heidelberg Engineering, Heidelberg, Germany); GA area was measured using the Region Finder (Heidelberg Engineering) software native to the Spectralis platform. MAIN OUTCOME MEASURES Area and increase in area of GA. RESULTS A total of 97 eyes were examined. Vitreoretinal traction was found in 39 eyes (40%). The GA area at baseline was 6.65±5.64 mm(2) in eyes with VRT and 5.73±4.72 mm(2) in eyes with no VRT. The annual rate of progression of GA area progression was 2.99±0.66 mm(2) in eyes with VRT and 1.45±0.67mm(2) in eyes without VRT. Differences between groups in both parameters were statistically significant (n = 97 total number of eyes; P<0.001). Multiple regression analysis confirmed this finding (B = 0.714, P<0.001; F3,93 = 72.542, P<0.001; adjusted R(2) = 0.691) CONCLUSIONS: Our results indicate an association between VRT and an increased rate of progression of GA area in dry AMD. Monitoring VRT may contribute to an improved estimate of the prospective time of visual loss and to a better timing of emerging therapies in dry AMD.

Quantification of water content across a cement-clay interface using high resolution neutron radiography

In many designs for radioactive waste repositories, cement and clay will come into direct contact. The geochemical contrast between cement and clay will lead to mass fluxes across the interface, which consequently results in alteration of structural and transport properties of both materials that may affect the performance of the multi-barrier system. We present an experimental approach to study cement-clay interactions with a cell to accommodate small samples of cement and clay. The cell design allows both in situ measurement of water content across the sample using neutron radiography and measurement of transport parameters using through-diffusion tracer experiments. The aim of the high- resolution neutron radiography experiments was to monitor changes in water content (porosity) and their spatial extent. Neutron radiographs of several evolving cement-clay interfaces delivered quantitative data which allow resolving local water contents within the sample domain. In the present work we explored the uncertainties of the derived water contents with regard to various input parameters and with regard to the applied image correction procedures. Temporal variation of measurement conditions created absolute uncertainty of the water content in the order of ±0.1 (m3/m3), which could not be fully accounted for by correction procedures. Smaller relative changes in water content between two images can be derived by specific calibrations to two sample regions with different, invariant water contents.

A multimodal tablet-based interface for designing and reviewing 3D engineering models

The ability to view and interact with 3D models has been happening for a long time. However, vision-based 3D modeling has only seen limited success in applications, as it faces many technical challenges. Hand-held mobile devices have changed the way we interact with virtual reality environments. Their high mobility and technical features, such as inertial sensors, cameras and fast processors, are especially attractive for advancing the state of the art in virtual reality systems. Also, their ubiquity and fast Internet connection open a path to distributed and collaborative development. However, such path has not been fully explored in many domains. VR systems for real world engineering contexts are still difficult to use, especially when geographically dispersed engineering teams need to collaboratively visualize and review 3D CAD models. Another challenge is the ability to rendering these environments at the required interactive rates and with high fidelity. In this document it is presented a virtual reality system mobile for visualization, navigation and reviewing large scale 3D CAD models, held under the CEDAR (Collaborative Engineering Design and Review) project. It’s focused on interaction using different navigation modes. The system uses the mobile device's inertial sensors and camera to allow users to navigate through large scale models. IT professionals, architects, civil engineers and oil industry experts were involved in a qualitative assessment of the CEDAR system, in the form of direct user interaction with the prototypes and audio-recorded interviews about the prototypes. The lessons learned are valuable and are presented on this document. Subsequently it was prepared a quantitative study on the different navigation modes to analyze the best mode to use it in a given situation.

A speech interface for air traffic control terminals

Several issues concerning the current use of speech interfaces are discussed and the design and development of a speech interface that enables air traffic controllers to command and control their terminals by voice is presented. A special emphasis is made in the comparison between laboratory experiments and field experiments in which a set of ergonomics-related effects are detected that cannot be observed in the controlled laboratory experiments. The paper presents both objective and subjective performance obtained in field evaluation of the system with student controllers at an air traffic control (ATC) training facility. The system exhibits high word recognition test rates (0.4% error in Spanish and 1.5% in English) and low command error (6% error in Spanish and 10.6% error in English in the field tests). Subjective impression has also been positive, encouraging future development and integration phases in the Spanish ATC terminals designed by Aeropuertos Españoles y Navegación Aérea (AENA).

Using the Journalistic Metaphor to Design User Interfaces That Explain Sensor Data

Facilitating general access to data from sensor networks (including traffic, hydrology and other domains) increases their utility. In this paper we argue that the journalistic metaphor can be effectively used to automatically generate multimedia presentations that help non-expert users analyze and understand sensor data. The journalistic layout and style are familiar to most users. Furthermore, the journalistic approach of ordering information from most general to most specific helps users obtain a high-level understanding while providing them the freedom to choose the depth of analysis to which they want to go. We describe the general characteristics and architectural requirements for an interactive intelligent user interface for exploring sensor data that uses the journalistic metaphor. We also describe our experience in developing this interface in real-world domains (e.g., hydrology).

The determinants of the quality of Sales-Marketing Interface in a Multinational Customer Brand Focused Company: The Latin American Branches

Customer evolution and changes in consumers, determine the fact that the quality of the interface between marketing and sales may represent a true competitive advantage for the firm. Building on multidimensional theoretical and empirical models developed in Europe and on social network analysis, the organizational interface between the marketing and sales departments of a multinational high-growth company with operations in Argentina, Uruguay and Paraguay is studied. Both, attitudinal and social network measures of information exchange are used to make operational the nature and quality of the interface and its impact on performance. Results show the existence of a positive relationship of formalization, joint planning, teamwork, trust and information transfer on interface quality, as well as a positive relationship between interface quality and business performance. We conclude that efficient design and organizational management of the exchange network are essential for the successful performance of consumer goods companies that seek to develop distinctive capabilities to adapt to markets that experience vertiginous changes

Integration of design tools and knowledge capture into a CAD system: a case study

onceptual design phase is partially supported by product lifecycle management/computer-aided design (PLM/CAD) systems causing discontinuity of the design information flow: customer needs — functional requirements — key characteristics — design parameters (DPs) — geometric DPs. Aiming to address this issue, it is proposed a knowledge-based approach is proposed to integrate quality function deployment, failure mode and effects analysis, and axiomatic design into a commercial PLM/CAD system. A case study, main subject of this article, was carried out to validate the proposed process, to evaluate, by a pilot development, how the commercial PLM/CAD modules and application programming interface could support the information flow, and based on the pilot scheme results to propose a full development framework.

Design and Validation of a Light Inference System to Support Embedded Context Reasoning

Embedded context management in resource-constrained devices (e.g. mobile phones, autonomous sensors or smart objects) imposes special requirements in terms of lightness for data modelling and reasoning. In this paper, we explore the state-of-the-art on data representation and reasoning tools for embedded mobile reasoning and propose a light inference system (LIS) aiming at simplifying embedded inference processes offering a set of functionalities to avoid redundancy in context management operations. The system is part of a service-oriented mobile software framework, conceived to facilitate the creation of context-aware applications?it decouples sensor data acquisition and context processing from the application logic. LIS, composed of several modules, encapsulates existing lightweight tools for ontology data management and rule-based reasoning, and it is ready to run on Java-enabled handheld devices. Data management and reasoning processes are designed to handle a general ontology that enables communication among framework components. Both the applications running on top of the framework and the framework components themselves can configure the rule and query sets in order to retrieve the information they need from LIS. In order to test LIS features in a real application scenario, an ?Activity Monitor? has been designed and implemented: a personal health-persuasive application that provides feedback on the user?s lifestyle, combining data from physical and virtual sensors. In this case of use, LIS is used to timely evaluate the user?s activity level, to decide on the convenience of triggering notifications and to determine the best interface or channel to deliver these context-aware alerts.

Design of a remote controlled hovercraft

Este informe trata el diseño, desarrollo y construcción de un aerodeslizador de pequeño tamaño, equipado con control remoto que permite al usuario actuar sobre la velocidad y dirección del mismo. Este proyecto podrá ser utilizado en un futuro como base para el desarrollo de aplicaciones más complejas. Un aerodeslizador es un medio de transporte cuyo chasis se eleva sobre el suelo por medio de un motor impulsor que hincha una falda colocada en la parte inferior del mismo. Además, uno o más motores se colocan en la parte trasera del vehículo para propulsarlo. El hecho de que el aerodeslizador no este en contacto directo con la tierra, hace que pueda moverse tanto por tierra como sobre el agua o hielo y que sea capaz de superar pequeños obstáculos. Por otra parte, este hecho se convierte a su vez en un problema debido a que su fuerza de rozamiento al desplazarse es muy pequeña, lo que provoca que sea muy difícil de frenar, y tienda a girar por sí mismo debido a la inercia del movimiento y a las fuerzas provocadas por las corrientes de aire debajo del chasis. Sin embargo, para este proyecto no se ha colocado una falda debajo del mismo, debido a que su diseño es bastante complicado, por lo tanto la fricción con el suelo es menor, aumentando los problemas detallados con anterioridad. El proyecto consta de dos partes, mando a distancia y aerodeslizador, que se conectan a través de antenas de radiofrecuencia (RF). El diseño y desarrollo de cada una ha sido realizado de manera separada exceptuando la parte de las comunicaciones entre ambas. El mando a distancia se divide en tres partes. La primera está compuesta por la interfaz de usuario y el circuito que genera las señales analógicas correspondientes a sus indicaciones. La interfaz de usuario la conforman tres potenciómetros: uno rotatorio y dos deslizantes. El rotatorio se utiliza para controlar la dirección de giro del aerodeslizador, mientras que cada uno de los deslizantes se emplea para controlar la fuerza del motor impulsor y del propulsor respectivamente. En los tres casos los potenciómetros se colocan en el circuito de manera que actúan como divisores de tensión controlables. La segunda parte se compone de un microcontrolador de la familia PSoC. Esta familia de microcontroladores se caracteriza por tener una gran adaptabilidad a la aplicación en la que se quieran utilizar debido a la posibilidad de elección de los periféricos, tanto analógicos como digitales, que forman parte del microcontrolador. Para el mando a distancia se configura con tres conversores A/D que se encargan de transformar las señales procedentes de los potenciómetros, tres amplificadores programables para trabajar con toda la escala de los conversores, un LCD que se utiliza para depurar el código en C con el que se programa y un módulo SPI que es la interfaz que conecta el microcontrolador con la antena. Además, se utilizan cuatro pines externos para elegir el canal de transmisión de la antena. La tercera parte es el módulo transceptor de radio frecuencia (RF) QFM-TRX1-24G, que en el mando a distancia funciona como transmisor. Éste utiliza codificación Manchester para asegurar bajas tasas de error. Como alimentación para los circuitos del mando a distancia se utilizan cuatro pilas AA de 1,5 voltios en serie. En el aerodeslizador se pueden distinguir cinco partes. La primera es el módulo de comunicaciones, que utiliza el mismo transceptor que en el mando a distancia, pero esta vez funciona como receptor y por lo tanto servirá como entrada de datos al sistema haciendo llegar las instrucciones del usuario. Este módulo se comunica con el siguiente, un microcontrolador de la familia PSoC, a través de una interfaz SPI. En este caso el microcontrolador se configura con: un modulo SPI, un LCD utilizado para depurar el código y tres módulos PWM (2 de 8 bits y uno de 16 bits) para controlar los motores y el servo del aerodeslizador. Además, se utilizan cuatro pines externos para seleccionar el canal de recepción de datos. La tercera y cuarta parte se pueden considerar conjuntamente. Ambas están compuestas por el mismo circuito electrónico basado en transistores MOSFET. A la puerta de cada uno de los transistores llega una señal PWM de 100 kilohercios que proviene del microcontrolador, que se encarga de controlar el modo de funcionamiento de los transistores, que llevan acoplado un disipador de calor para evitar que se quemen. A su vez, los transistores hacen funcionar al dos ventiladores, que actúan como motores, el impulsor y el propulsor del aerodeslizador. La quinta y última parte es un servo estándar para modelismo. El servo está controlado por una señal PWM, en la que la longitud del pulso positivo establece la posición de la cabeza del servo, girando en uno u otra dirección según las instrucciones enviadas desde el mando a distancia por el usuario. Para el aerodeslizador se han utilizado dos fuentes de alimentación diferentes: una compuesta por 4 pilas AA de 1,5 voltios en serie que alimentarán al microcontrolador y al servo, y 4 baterías de litio recargables de 3,2 voltios en serie que alimentan el circuito de los motores. La última parte del proyecto es el montaje y ensamblaje final de los dispositivos. Para el chasis del aerodeslizador se ha utilizado una cubierta rectangular de poli-estireno expandido, habitualmente encontrado en el embalaje de productos frágiles. Este material es bastante ligero y con una alta resistencia a los golpes, por lo que es ideal para el propósito del proyecto. En el chasis se han realizado dos agujeros: uno circular situado en el centro del mismo en el se introduce y se ajusta con pegamento el motor impulsor, y un agujero con la forma del servo, situado en uno del los laterales estrechos del rectángulo, en el que se acopla el mismo. El motor propulsor está adherido al cabezal giratorio del servo de manera que rota a la vez que él, haciendo girar al aerodeslizador. El resto de circuitos electrónicos y las baterías se fijan al chasis mediante cinta adhesiva y pegamento procurando en todo momento repartir el peso de manera homogénea por todo el chasis para aumentar la estabilidad del aerodeslizador. SUMMARY: In this final year project a remote controlled hovercraft was designed using mainly technology that is well known by students in the embedded systems programme. This platform could be used to develop further and more complex projects. The system was developed dividing the work into two parts: remote control and hovercraft. The hardware was of the hovercraft and the remote control was designed separately; however, the software was designed at the same time since it was needed to develop the communication system. The result of the project was a remote control hovercraft which has a user friendly interface. The system was designed based on microprocessor technologies and uses common remote control technologies. The system has been designed with technology commonly used by the students in Metropolia University so that it can be readily understood in order to develop other projects based on this platform.