Effects of oligotrophication on primary production in peri-alpine lakes

During the second half of the 20th century untreated sewage released from housing and industry into natural waters led to a degradation of many freshwater lakes and reservoirs worldwide. In order to mitigate eutrophication, wastewater treatment plants, including Fe-induced phosphorus precipitation, were implemented throughout the industrialized world, leading to reoligotrophication in many freshwater lakes. To understand and assess the effects of reoligotrophication on primary productivity, we analyzed 28 years of 14C assimilation rates, as well as other biotic and abiotic parameters, such as global radiation, nutrient concentrations and plankton densities in peri-alpine Lake Lucerne, Switzerland. Using a simple productivity-light relationship, we estimated continuous primary production and discussed the relation between productivity and observed limnological parameters. Furthermore, we assessed the uncertainty of our modeling approach based on monthly 14C assimilation measurements using Monte Carlo simulations. Results confirm that monthly sampling of productivity is sufficient for identifying long-term trends in productivity and that conservation management has successfully improved water quality during the past three decades via reducing nutrients and primary production in the lake. However, even though nutrient concentrations have remained constant in recent years, annual primary production varies significantly from year to year. Despite the fact that nutrient concentrations have decreased by more than an order of magnitude, primary production has decreased only slightly. These results suggest that primary production correlates well to nutrients availability but meteorological conditions lead to interannual variability regardless of the trophic status of the lake. Accordingly, in oligotrophic freshwaters meteorological forcing may reduce productivity impacting on the entire food chain of the ecosystem.

Differential effects of intranasal vaccination with recombinant NcPDI in different mouse models of Neospora caninum infection

In this study, mice were vaccinated intranasally with recombinant N. caninum protein disulphide isomerase (NcPDI) emulsified in cholera toxin (CT) or cholera toxin subunit B (CTB) from Vibrio cholerae. The effects of vaccination were assessed in the murine nonpregnant model and the foetal infection model, respectively. In the nonpregnant mice, previous results were confirmed, in that intranasal vaccination with recNcPDI in CT was highly protective, and low cerebral parasite loads were noted upon real-time PCR analysis. Protection was accompanied by an IgG1-biased anti-NcPDI response upon infection and significantly increased expression of Th2 (IL-4/IL-10) and IL-17 transcripts in spleen compared with corresponding values in mice treated with CT only. However, vaccination with recNcPDI in CT did not induce significant protection in dams and their offspring. In the dams, increased splenic Th1 (IFN-γ/IL-12) and Th17 mRNA expressions was detected. No protection was noted in the groups vaccinated with recNcPDI emulsified in CTB. Thus, vaccination with recNcPDI in CT in nonpregnant mice followed by challenge infection induced a protective Th2-biased immune response, while in the pregnant mouse model, the same vaccine formulation resulted in a Th1-biased inflammatory response and failed to protect dams and their progeny.

A 2000 year long seasonal record of floods in the southern European Alps

Knowledge of past natural flood variability and controlling climate factors is of high value since it can be useful to refine projections of the future flood behavior under climate warming. In this context, we present a seasonally resolved 2000 year long flood frequency and intensity reconstruction from the southern Alpine slope (North Italy) using annually laminated (varved) lake sediments. Floods occurred predominantly during summer and autumn, whereas winter and spring events were rare. The all-season flood frequency and, particularly, the occurrence of summer events increased during solar minima, suggesting solar-induced circulation changes resembling negative conditions of the North Atlantic Oscillation as controlling atmospheric mechanism. Furthermore, the most extreme autumn events occurred during a period of warm Mediterranean sea surface temperature. Interpreting these results in regard to present climate change, our data set proposes for a warming scenario, a decrease in summer floods, but an increase in the intensity of autumn floods at the South-Alpine slope.

Tracking water pathways in steep hillslopes by d18O depth profiles of soil water

Assessing temporal variations in soil water flow is important, especially at the hillslope scale, to identify mechanisms of runoff and flood generation and pathways for nutrients and pollutants in soils. While surface processes are well considered and parameterized, the assessment of subsurface processes at the hillslope scale is still challenging since measurement of hydrological pathways is connected to high efforts in time, money and personnel work. The latter might not even be possible in alpine environments with harsh winter processes. Soil water stable isotope profiles may offer a time-integrating fingerprint of subsurface water pathways. In this study, we investigated the suitability of soil water stable isotope (d18O) depth profiles to identify water flow paths along two transects of steep subalpine hillslopes in the Swiss Alps. We applied a one-dimensional advection–dispersion model using d18O values of precipitation (ranging from _24.7 to _2.9‰) as input data to simulate the d18O profiles of soil water. The variability of d18O values with depth within each soil profile and a comparison of the simulated and measured d18O profiles were used to infer information about subsurface hydrological pathways. The temporal pattern of d18O in precipitation was found in several profiles, ranging from _14.5 to _4.0‰. This suggests that vertical percolation plays an important role even at slope angles of up to 46_. Lateral subsurface flow and/or mixing of soil water at lower slope angles might occur in deeper soil layers and at sites near a small stream. The difference between several observed and simulated d18O profiles revealed spatially highly variable infiltration patterns during the snowmelt periods: The d18O value of snow (_17.7 ± 1.9‰) was absent in several measured d18O profiles but present in the respective simulated d18O profiles. This indicated overland flow and/or preferential flow through the soil profile during the melt period. The applied methods proved to be a fast and promising tool to obtain time-integrated information on soil water flow paths at the hillslope scale in steep subalpine slopes.

Is "milk crust" a transient form of golden retriever ichthyosis?

BACKGROUND A recessive inherited form of lamellar ichthyosis is well recognized in golden retrievers. In this breed, young puppies demonstrate a self-limiting scaling disorder which is commonly recognized by breeders, who use the term "milk crust" to describe this syndrome. HYPOTHESIS/OBJECTIVES To determine whether "milk crust" is a new keratinization disorder or a self-limiting form of golden retriever ichthyosis. ANIMALS A total of 179 golden retriever dogs (21 dams and 158 puppies) were examined. METHODS Dermatological examination and assessment of the patatin-like phospholipase-1 (PNPLA1) genotype by PCR testing of buccal mucosal swabs. Skin biopsies from one affected puppy were evaluated for histopathological abnormalities. RESULTS Forty-five of 158 (28%) puppies exhibited scaling at 8 weeks of age; 113 of 158 (72%) were dermatologically normal. Of 144 analysed samples, 40 of 144 (28%) puppies demonstrated a homozygous mutation of the PNPLA1 genotype [of which, 36 of 40 (90%) had signs of scaling], 77 of 144 (53%) demonstrated a heterozygous mutation and 27 of 144 (19%) were a normal wild-type. In six of 17 (35%) dams, a homozygous mutation of the PNPLA1 genotype was found, eight of 17 (47%) demonstrated a heterozygous mutation and three of 17 (18%) were normal wild-type. Dams with a homozygous mutation were clinically unaffected. A 1 year follow-up revealed that 23 of 28 (82%) puppies affected with this syndrome failed to develop typical signs of ichthyosis. In five of 28 (18%) dogs there was persistence of mild scaling. CONCLUSIONS AND CLINICAL IMPORTANCE We hypothesize that the clinical syndrome termed "milk crust" could represent a transient form of golden retriever ichthyosis. Remission is not fully linked to PNPLA1 genotype, suggesting that unknown factors may contribute to the clinical disease.

Dose-dependent effects of experimental infection with the virulent Neospora caninum Nc-Spain7 isolate in a pregnant mouse model.

Pregnant BALB/c mice have been widely used as an in vivo model to study Neospora caninum infection biology and to provide proof-of-concept for assessments of drugs and vaccines against neosporosis. The fact that this model has been used with different isolates of variable virulence, varying infection routes and differing methods to prepare the parasites for infection, has rendered the comparison of results from different laboratories impossible. In most studies, mice were infected with similar number of parasites (2 × 10(6)) as employed in ruminant models (10(7) for cows and 10(6) for sheep), which seems inappropriate considering the enormous differences in the weight of these species. Thus, for achieving meaningful results in vaccination and drug efficacy experiments, a refinement and standardization of this experimental model is necessary. Thus, 2 × 10(6), 10(5), 10(4), 10(3) and 10(2) tachyzoites of the highly virulent and well-characterised Nc-Spain7 isolate were subcutaneously inoculated into mice at day 7 of pregnancy, and clinical outcome, vertical transmission, parasite burden and antibody responses were compared. Dams from all infected groups presented nervous signs and the percentage of surviving pups at day 30 postpartum was surprisingly low (24%) in mice infected with only 10(2) tachyzoites. Importantly, infection with 10(5) tachyzoites resulted in antibody levels, cerebral parasite burden in dams and 100% mortality rate in pups, which was identical to infection with 2 × 10(6) tachyzoites. Considering these results, it is reasonable to lower the challenge dose to 10(5) tachyzoites in further experiments when assessing drugs or vaccine candidates.

Dieta de verano de bovinos pastoreando en bosques de lenga (Nothofagus pumilio) y mallines de Chubut, Argentina

Se determinó la composición de la dieta de verano de bovinos pastoreando en sistemas formados por bosques de Nothofagus pumilio (lenga) y mallines, del noroeste del Chubut. Se estimó la disponibilidad forrajera de los mallines y del sotobosque mediante cortes de biomasa aérea, y se determinó la composición dietaria mediante análisis microhistológico de heces. La dieta está integrada principalmente por especies de gramíneas y graminoides, que disminuyeron su frecuencia al final del verano, mientras que los renovales de lenga y otras especies leñosas la aumentaron. El ganado selecciona gramíneas en ambos períodos, y al final del verano también los renovales de lenga. La ganadería constituye una importante y creciente forma de subsistencia para los pobladores rurales del noroeste del Chubut. Este trabajo aporta elementos para avanzar en la búsqueda de herramientas que permitan la sustentabilidad de los bosques de lenga.

Sediment volume variations inferred from DGPS measurements within Bossons glacier proglacial area (Mont-Blanc massif, France)

This dataset presents Differential Global Positioning System data (DGPS) acquired within the Bossons glacier proglacial area. Bossons glacier is a rapidly retreating glacier and its proglacial area is deglaciated for ~30 years. Bossons stream is one of the outlets of the subglacial drainage system. It starts as a 800 m steep cascade reach, then flows through an area with gentler slope : the Plan des Eaux (PdE). PdE is a 300 m long, 50 m wide proglacial alluvial plain with an increasing channel mobility in the downstream direction but decreasing slope gradient and incision. As it may act a sediment trap, studying periglacial and proglacial erosion processes in the Bossons catchment requires to quantify PdE sediment volume evolution. A several meter-sized block located within Bossons proglacial area was set up as GPS base : its location was measured by one antenna (Topcon Hyper Pro) by performing 600 consecutive measurements throughout one day. A second antenna (Topcon Hyper Pro) was then used to measure XYZ location of points in the proglacial area with a ~2 m grid. Radio communication between the two antennas allowed differential calculations to be automatically carried out on field using the Topcon FC-250 hand controller. This methodology yields 3 cm XY and 1.5 cm Z uncertainties. DGPS data have been acquired through 10 campaigns from 2004 to 2014; campaigns from 2004 to 2008 cover a smaller area than those from 2010 to 2014. Digital Elevation Model (DEM) have been interpolated from DGPS data and difference between two DEMs yields deposited and eroded volume within PdE. Maps of PdE volume variation between two campaigns show that incision mainly occurs in the upper and lower sections where as deposition dominates in the middle section. Deposition, denudation and net rate (deposition rate - denudation rate) are calculated by normalizing volumes by DEM areas. Deposition dominates results with a mean net rate of 29 mm/yr. However, strong inter-annual variability exists and some years are dominated by denudation : -36 mm/yr and -100 mm/yr for 2006 and 2011, respectively. Nonetheless, oldest campaigns (2004 to 2008) were carried out on the lower part part of the alluvial plain and ruling them out to keep only complete DEM (2010 to 2014) yields a mean net rate of ~15 mm/yr. This results is coherent with field observations of both strong deposition (e.g. flood deposits) and strong erosion (e.g. 30 cm incision) evidences. Bossons glacier proglacial area is thus dynamic with year-to-year geormorphological changes but may leans toward increasing its mean elevation through a deposition dominated system.

Spatially explicit methane inventory for Switzerland with 500 m x 500 m resolution for 2011 (NetCDF 25 MB)

We present the first high-resolution (500 m × 500 m) gridded methane (CH4) emission inventory for Switzerland, which integrates the national emission totals reported to the United Nations Framework Convention on Climate Change (UNFCCC) and recent CH4 flux studies conducted by research groups across Switzerland. In addition to anthropogenic emissions, we also include natural and semi-natural CH4 fluxes, i.e., emissions from lakes and reservoirs, wetlands, wild animals as well as uptake by forest soils. National CH4 emissions were disaggregated using detailed geostatistical information on source locations and their spatial extent and process- or area-specific emission factors. In Switzerland, the highest CH4 emissions in 2011 originated from the agricultural sector (150 Gg CH4/yr), mainly produced by ruminants and manure management, followed by emissions from waste management (15 Gg CH4/yr) mainly from landfills and the energy sector (12 Gg CH4/yr), which was dominated by emissions from natural gas distribution. Compared to the anthropogenic sources, emissions from natural and semi-natural sources were relatively small (6 Gg CH4/yr), making up only 3 % of the total emissions in Switzerland. CH4 fluxes from agricultural soils were estimated to be not significantly different from zero (between -1.5 and 0 Gg CH4/yr), while forest soils are a CH4 sink (approx. -2.8 Gg CH4/yr), partially offsetting other natural emissions. Estimates of uncertainties are provided for the different sources, including an estimate of spatial disaggregation errors deduced from a comparison with a global (EDGAR v4.2) and a European CH4 inventory (TNO/MACC). This new spatially-explicit emission inventory for Switzerland will provide valuable input for regional scale atmospheric modeling and inverse source estimation.

Magnetic properties of ODP Hole 116-717C

Dark gray and black mud turbidites cored on ODP Leg 116 commonly yielded large magnetic susceptibility peaks. What is more, these peaks displayed different shapes suggesting variations in sedimentological processes. Consequently, a detailed study of the magnetic properties of two of these turbidites was undertaken to better understand the source of their unusual magnetism. Physical properties were measured as was the demagnetization behavior of sample natural remanent magnetizations (NRMs). Subsequently, an anhysteretic remanent magnetization (ARM) and saturation isothermal remanent magnetization (SIRM) were imparted to the samples, demagnetized, and various grain size tests based on the behavior of these remanences were applied. Finally, magnetic concentrates from two samples were examined with a scanning electron microscope with the capability to do energy dispersive X-ray (EDX) analysis. The turbidites stand out from surrounding layers because of their high susceptibilities, NRMs, ARMs, SIRMs, and ratios of ARM and SIRM to susceptibility. Their alternating field and thermal demagnetization properties and IRM acquisition curves are consistent with titanomagnetite grains as the primary magnetic mineral with some amount of hematite present. These properties are very similar to those published for samples from the Deccan flood basalts and suggest this formation as a possible source of the magnetic grains. Magnetic granulometry tests implied that the magnetic particles behave dominantly as single-domain and pseudo-single-domain grains. Moreover, they also implied that the large variation in susceptibility observed in the black mud turbidites results from a tenfold increase in the concentration of titanomagnetite grains. Electron microscope, EDX, and SIRM analyses revealed detrital titanomagnetites with typical sizes around 8-10 µm, but as large as 20-25 µm. These are probably the dominant magnetic grains in the black mud turbidites; however, ARM and susceptibility frequency-dependence suggested that there may also be a submicrometer fraction present. Most of the observed titanomagnetite grains are tabular and some display exsolution lamellae, accounting for the pseudo-single-domain behavior despite their moderate sizes. We hypothesize that the magnetic mineral concentration variations are brought about by sedimentological factors. The heavier magnetic minerals may tend to sink to the bottom of a turbidite; however, sometimes turbidite turbulence may act to keep these tabular, medium-size grains in suspension longer than some other larger or more equidimensional grains. Consequently, the susceptibility peak shape may reflect the turbidite current velocities as well as other sedimentological factors.

A 2000 year long seasonal record of floods in Lake Ledro

Knowledge of past natural flood variability and controlling climate factors is of high value since it can be useful to refine projections of the future flood behavior under climate warming. In this context, we present a seasonally resolved 2000 year long flood frequency and intensity reconstruction from the southern Alpine slope (North Italy) using annually laminated (varved) lake sediments. Floods occurred predominantly during summer and autumn, whereas winter and spring events were rare. The all-season flood frequency and, particularly, the occurrence of summer events increased during solar minima, suggesting solar-induced circulation changes resembling negative conditions of the North Atlantic Oscillation as controlling atmospheric mechanism. Furthermore, the most extreme autumn events occurred during a period of warm Mediterranean sea surface temperature. Interpreting these results in regard to present climate change, our data set proposes for a warming scenario, a decrease in summer floods, but an increase in the intensity of autumn floods at the South-Alpine slope.

2012-2014 sedimentation data for the Congaree River floodplain, SC, USA

This dataset contains sedimentation data that was collected for the Congaree River floodplain, SC, USA. The study sites were located at floodplain positions with varying flood frequencies and geomorphic positions. Flood frequency was determined using hydrological statistics based on data acquired from USGS gage #02169500 for the Congaree River; sites are classified as having high, intermediate, or low flood frequency. The geomorphic position classification was determined using field observations; sites are classified as abandoned channel, ridge, river margin, and swale positions. Sediment deposition was measured in centimeters and reflects the amount of sediment that was deposited on a feldspar clay pad that was installed at each of the sampling sites. The feldspar pads were installed in the summer of 2012 and cored and measured for deposition amounts in the summer of 2014. In the summer of 2012, soil samples were collected at each of the sampling sites. Each soil sample was analyzed for soil texture (percent sand, silt, and clay), major and micro-nutrients (parts per million), percent organic matter, and pH.

Exceptionally well-preserved vegetal remains from the Upper Cretaceous of "Lo Hueco", Cuenca, Spain

Vegetal remains are considered labile structures that quickly become decayed in ecosystems. However, certain lignified tissues (woody plants) can largely resist decomposition, becoming sometimes exceptionally well preserved. At the Upper Cretaceous site of ?Lo Hueco? (Cuenca, Spain), those woody remains (trunks and branches) with resinous material in the inner tracheids and parenchyma cells that were buried rapidly under anoxic conditions experienced a low degree of maturation, becoming exceptionally well preserved. Those woody remains deposited under oxic conditions (subaerial or sub-aquatic exposure) were more intensely biodegraded and subsequently carbonified, partially or completely mineralized in gypsum and covered by a ferruginous crust. These two modes of preservation are scarce, with silicification or carbonification processes much more common, and both can be considered as ?exceptional preservation?. Other vegetal remains, such as carbonified leaves, stems and roots, were collected in the site. The different modes of preservation depend directly on: depositional micro-environment (sandy distributary channel, muddy flood plain); and type (trunk, branch, stem, leave, root) and state (presence or absence of resinous material) of the material. The great abundance and diversity of fossils in ?Lo Hueco? identify it as Konzentrat-Lagersta¿tten, sequentially formed by alternated events of flooding and drying depositional events, but the exceptional quality and rarity of determinate vegetal macroremains preservation suggest that certain deposits of this site can be considered as conservation deposits.