Exogenous Slit2 does not affect ureteric branching or nephron formation during kidney development

In an attempt to elucidate the role of Slit2 invertebrate kidney development, the effect of adding exogenous human Slit2 protein (hSlit2) to developing murine metanephric kidney explants was examined. To confirm the activity of the recombinant Slit2 protein, neurons from 8 day old chick sympathetic nerve chain dorsal root ganglia were cultured with hSlit2 protein, which induced significant neurite branching and outgrowth. Using kidney explants as a model system, metanephric development in the presence of hSlit2 protein was examined. Addition of hSlit2 up to a final concentration of 1 mug/ml had no detectable effect on the formation of nephrons or on branching morphogenesis of the ureteric tree after 2 or 4 days in culture, as assessed via immunofluorescence for the markers WT1 and calbindin 28K respectively. Similarly, maturation of the nephrogenic mesenchyme occurred in a phenotypically normal fashion. In situ analysis of the Slit receptors, Robot and Robot, the vasculogenic markers VEGFA and Flk-1, and the stromal cell marker BF2 displayed no difference in comparison to controls.

Movement through slits: cellular migration via the Slit family

First isolated in the fly and now characterised in vertebrates, the Slit proteins have emerged as pivotal components controlling the guidance of axonal growth cones and the directional migration of neuronal precursors. As well as extensive expression during development of the central nervous system (CNS), the Slit proteins exhibit a striking array of expression sites in non-neuronal tissues, including the urogenital system, limb primordia and developing eye. Zebrafish Slit has been shown to mediate mesodermal migration during gastrulation, while Drosophila slit guides the migration of mesodermal cells during myogenesis. This suggests that the actions of these secreted molecules are not simply confined to the sphere of CNS development, but rather act in a more general fashion during development and throughout the lifetime of an organism. This review focuses on the non-neuronal activities of Slit proteins, highlighting a common role for the Slit family in cellular migration.

Sox18 mutations in the ragged mouse alleles ragged-like and opossum

The ragged (Ra) spontaneous mouse mutant is characterised by abnormalities in its coat and cardiovascular system. Four alleles are known and we have previously described mutations in the transcription factor gene Sox18 in the Ra and Ra-J alleles. We report here Sox18 mutations in the remaining two ragged alleles, opossum (Ra-op) and ragged-like (Ragl). The single-base deletions cause a C-terminal frameshift, abolishing transcriptional trans-activation and impairing interaction with the partner protein MEF2C. The nature of these mutations, together with the near-normal phenotype of Sox18-null mice, suggests that the ragged mutant SOX18 proteins act in a dominant-negative fashion. The four ragged mutants represent an allelic series that reveal SOX18 structure-function relationships and implicate related SOX proteins in cardiovascular and hair follicle development. (C) 2003 Wiley-Liss, Inc.

Antigen-specific suppression of a primed immune response by dendritic cells mediated by regulatory T cells secreting interleukin-10

Antigen-specific suppression of a previously primed immune response is a major challenge for immunotherapy of autoimmune disease. ReIB activation is required for myeloid DC differentiation. Here, we show that antigen-exposed DCs in which ReIB function is inhibited lack cell surface CD40, prevent priming of immunity, and suppress previously primed immune responses. DCs generated from CD40-deficient mice similarly confer suppression. Regulatory CD4(+) T cells induced by the DCs transfer antigen-specific Infectious tolerance to primed recipients in an interleukin10-dependent fashion. Thus CD40, regulated by ReIB activity, determines the consequences of antigen presentation by myeloid DCs. These observations have significance for autoimmune immunotherapy and suggest a mechanism by which peripheral tolerance might be constitutively maintained by RelB(-) CD40(-) DCs.

Putting the group back into unions: A social psychological contribution to understanding union support

Industrial relations research that attempts to grapple with individuals' union-related sentiments and activities often draws on one of two traditions of psychological research—the individual-level factors tradition (for example, personality and attitude-behaviour relations) and the social context tradition (for example, frustration-aggression and relative deprivation). This paper provides an overview of research conducted from within these traditions to explain union-related phenomena and identifies some of the limitations that arise as a consequence of a shared tendency to treat people in an atomistic fashion. The paper argues for an understanding of the psychological processes that underpin group-based action. To this end, it elaborates a theoretical framework based on social identity theory and self-categorisation theory that would allow us to examine the dynamic interplay between the individual, their cognitions and their environment. The paper concludes with a brief discussion of a specific case of union mobilisation, to indicate how this theoretical framework might aid empirical analysis.

Cytotoxic iron chelators: Characterization of the structure, solution chemistry and redox activity of ligands and iron complexes of the di-2-pyridyl ketone isonicotinoyl hydrazone (HPKIH) analogues

Di-2-pyridyl ketone isonicotinoyl hydrazone (HPKIH) and a range of its analogues comprise a series of monobasic acids that are capable of binding iron (Fe) as tridentate (N,N,O) ligands. Recently, we have shown that these chelators are highly cytotoxic, but show selective activity against cancer cells. Particularly interesting was the fact that cytotoxicity of the HPKIH analogues is maintained even after complexation with Fe. To understand the potent anti-tumor activity of these compounds, we have fully characterized their chemical properties. This included examination of the solution chemistry and X-ray crystal structures of both the ligands and Fe complexes from this class and the ability of these complexes to mediate redox reactions. Potentiometric titrations demonstrated that all chelators are present predominantly in their charge-neutral form at physiological pH (7.4), allowing access across biological membranes. Keto-enol tautomerism of the ligands was identified, with the tautomers exhibiting distinctly different protonation constants. Interestingly, the chelators form low-spin (diamagnetic) divalent Fe complexes in solution. The chelators form distorted octahedral complexes with Fe-II, with two tridentate ligands arranged in a meridional fashion. Electrochemistry of the Fe complexes in both aqueous and non-aqueous solutions revealed that the complexes are oxidized to their ferric form at relatively high potentials, but this oxidation is coupled to a rapid reaction with water to form a hydrated (carbinolamine) derivative, leading to irreversible electrochemistry. The Fe complexes of the HPKIH analogues caused marked DNA degradation in the presence of hydrogen peroxide. This observation confirms that Fe complexes from the HPKIH series mediate Fenton chemistry and do not repel DNA. Collectively, studies on the solution chemistry and structure of these HPKIH analogues indicate that they can bind cellular Fe and enhance its redox activity, resulting in oxidative damage to vital biomolecules.

A educação em cena : a Revista de Educação como circulação de representações sobre saberes educacionais no Espírito Santo (1934-1937)

Este estudo analisa os processos de circulação e apropriação das representações sobre os saberes da educação escolarizada difundidos pela Revista de Educação do Espírito Santo, entre os anos de 1934 e 1937. Como referencial teórico baseamo- nos em Chartier (1990) acerca do conceito de representação, em Balandier (1982) em relação ao conceito de encenação de poder instituído que assume visibilidade quando circula na Revista, concedendo publicização aos feitos políticos realizados por dado grupo social, e em Julia (2001), junto ao conceito de cultura escolar. A partir desse arcabouço teórico, empreendemos metodologia de pesquisa a partir da análise histórica da fonte, dialogando, para tanto, com diferentes documentos e registros que configuram uma série de dados que constituem nossa fonte. Trata-se da análise das representações travadas no debate sobre a formação, divulgação e apropriação do conjunto de práticas e saberes pedagógicos dirigido aos professores, por parte de um grupo de intelectuais locais que se apresentava como portador do projeto de modernização do Espírito Santo inserido no contexto nacional. A Revista de Educação/ES tinha entre seus principais objetivos o de (in)formar os professores, ou seja, enquadrar suas práticas às novas demandas educacionais. Deste modo, a Revista esteve atrelada a um projeto educacional em que os intelectuais corroboravam a ampla circulação de um conjunto de representações sobre a modernidade, utilizando a Revista como suporte, visto que, desde as capas, são expostos “monumentos de modernização”, como os prédios escolares e todo um complexo arranjo de artefatos simbólicos, traduzidos muitas vezes em festas e rituais escolares que evocavam “um novo tempo para a Educação do Estado”, ou seja, fazendo da educação escolarizada um espetáculo.

Entre o matrimônio, a beleza, a moda e esportes : imagens da mulher na Revista Vida Capichaba (1925-1939)

Essa dissertação tem por objetivo de estudo investigar as imagens do feminino presentes na revista Vida Capichaba, periódico de publicação quinzenal que circulou, no Estado do Espírito Santo, entre as décadas de 1920 e 1950. Diante da longevidade do periódico, optamos por concentrar nossos esforços analíticos nas décadas de 1920 e 1930, especificamente entre os anos de 1925 e 1939. Entendemos que o ideal de mulher presente na revista passou pela construção de requisitos morais e uma formação adequada para desempenhar papéis concebidos como naturalmente femininos como o casamento e a maternidade. Além dessa característica, outras práticas se fizeram ainda mais destacadas. A construção da beleza, de um corpo magro e jovem associado à valorização dos esportes e da moda, se tornaram características da nova mulher capixaba. Eficiência e delicadeza, sensualidade e obediência, maternidade e independência, agilidade e elegância, beleza e liberdade. São dualidades como essas, nas quais códigos sociais tradicionais e novos valores culturais se articulam definindo novos modos de ser, que compuseram o quadro de imagens presente na Vida Capichaba.

A guerrilha esquecida : memórias do Caparaó (1966-67), o primeiro foco guerrilheiro contra a ditadura militar no Brasil

Este estudo tem como objetivo discutir a memória histórica do primeiro foco guerrilheiro no Brasil, organizado pelo Movimento Nacional Revolucionário (MNR), em 1966-1967, com o apoio de Leonel Brizola e de Cuba, na região do Caparaó, divisa dos Estados de Minas Gerais e Espírito Santo. Assim, o estudo se propõe a analisar o pouco conhecido episódio da formação, do idealismo e do desfecho que envolveu a Guerrilha do Caparaó, apresentando as memórias dos guerrilheiros (sendo em sua grande maioria ex-militares), dos agentes da repressão, dos setores conservadores da sociedade e dos habitantes das comunidades do entorno do atual Parque Nacional do Caparaó, demonstrando, assim, as diferentes percepções e representações sobre a referida Guerrilha. Para tanto, será exposto todo o contexto histórico da época em questão. Como metodologia, utilizaremos o conceito de memória, que, em suas ramificações, abrangerá a história oral, além de análises bibliográficas, pesquisas em jornais do período (A Gazeta, O Globo, Jornal do Brasil, A Última Hora, Tribuna da Imprensa, Correio da Manhã, O Estado de São Paulo, Estado de Minas, O Diário da Tarde), em revistas (O Cruzeiro, Opinião e Revista Capixaba) e em documentos da Delegacia de Ordem Política e Social (DOPS) dos Arquivos Públicos dos estados do Espírito Santo e Minas Gerais, e documentos do Serviço Nacional de Informação (SNI), do Arquivo Nacional.

Physical consequences of falls in the elderly: a literature review from 1995 to 2010

In the last decade, population ageing has been registered as a global phenomenon. A relation exists between falling and ageing, since falling frequency increases significantly with age. In fact, one in three older adult falls annually. Although ageing is generically associated with decrease and degeneration of psychological and physical functions, it is still not common for the correct identification of risk factors to lead to a clinical prognosis of the elder being in risk of falling. Therefore, the goal of this review article is to identify, categorise and analyse typical ageing and fall factors mentioned in the literature as well as to quantify the number of times they were referenced. The research considered hundreds of publications, but analysis was then restricted to the 87 most pertinent articles written in English and published in journals or scientific magazines between 1995 and 2010. We concluded that falls among older adults can be characterised by the following: anatomic characteristics and physiological consequences of ageing; the pathologies that induce falls, which can be neurological, musculoskeletal, cardiovascular and other diseases; causes and risk factors of falls that can be behavioural, biological, environmental or socio-economic; type of physical consequences of falls, including fractures, bruises, injuries or other physical consequences; and strategies to prevent, mitigate or rehabilitate, which can be of a physical, environmental or behavioural nature.

Physical consequences of falls in the elderly: a literature review from 1995 to 2010

In the last decade, population ageing has been registered as a global phenomenon. A relation exists between falling and ageing, since falling frequency increases significantly with age. In fact, one in three older adult falls annually. Although ageing is generically associated with decrease and degeneration of psychological and physical functions, it is still not common for the correct identification of risk factors to lead to a clinical prognosis of the elder being in risk of falling. Therefore, the goal of this review article is to identify, categorise and analyse typical ageing and fall factors mentioned in the literature as well as to quantify the number of times they were referenced. The research considered hundreds of publications, but analysis was then restricted to the 87 most pertinent articles written in English and published in journals or scientific magazines between 1995 and 2010. We concluded that falls among older adults can be characterised by the following: anatomic characteristics and physiological consequences of ageing; the pathologies that induce falls, which can be neurological, musculoskeletal, cardiovascular and other diseases; causes and risk factors of falls that can be behavioural, biological, environmental or socio-economic; type of physical consequences of falls, including fractures, bruises, injuries or other physical consequences; and strategies to prevent, mitigate or rehabilitate, which can be of a physical, environmental or behavioural nature.

Ler em tempo livre durante o Estado Novo: A função do suplemento infantil Pim-Pam-Pum na formação da criança

RESUMO: Esta dissertação tem como objectivo compreender a influência da ideologia do Estado Novo nos conteúdos inseridos no suplemento infantil Pim-Pam-Pum. Visa ainda relacionar, num determinado momento temporal o suplemento infantil de um dos jornais mais lidos da época O Século, com a ideologia do Estado Novo. Procura identificar os valores incutidos nos números a analisar no Pim-Pam-Pum num período de dois anos. O período histórico em análise do suplemento infantil Pim-Pam-Pum compreende os exemplares entre 1953 a 1955. Como principais conclusões do estudo retira-se que o suplemento infantil procurava educar o seu público de forma lúdica. Por outro lado, transmitia e incutia em colaboração com a família os principais valores salazaristas. A imprensa escrita passa a ser uma arma de propaganda ideológica do Estado Novo numa tentativa de conter informação desfavorável ao regime perante as pressões internas e externas. Com a institucionalização da censura após a segunda Guerra Mundial, a vigilância e o exame das publicações dos organismos competentes em colaboração com a própria auto-censura dos jornalistas, simplificou-se. No entanto a imprensa procurou cumprir com a sua missão de divulgação cultural e lúdica através de jornais diários, revistas e suplementos infantis. ABSTRACT:The aim of this dissertation is to understand the influence of the ideology of the "Estado Novo" in the contents inserted in the children's supplement Pim-Pam-Pum. It also aims to relate, in a certain historical period, the children’s supplement of one of the most important newspapers of the time, "O Século", with the ideology of the "Estado Novo". In addition, it intends to identify the values ingrained in the numbers to be analysed in Pim-Pam-Pum in a period of two years. The period under analysis of the children's supplement Pim-Pam-Pum includes the numbers from 1953 to 1955. One of the main conclusions of this study is that the children's aforementioned supplement aimed to educate its audience in a joyful manner. On the other hand, it transmitted as well as inculcated with the families’ collaboration the main Salazarist values. The press becomes a weapon of ideological propaganda of the "Estado Novo" in an attempt to keep harmful information to the regime from the internal and external pressures. With the institucionalization of censorship after the Second World War, the careful surveillance of publications by the competent and relevant organisms in collaboration with the censorship made by the journalists themselves, was simplified. However, the press tried to accomplish its mission of cultural and playful diffusion by means of newspapers, magazines and children's supplements.

Self-interacting scalar field cosmologies: unified exact solutions and symmetries

We investigate a mechanism that generates exact solutions of scalar field cosmologies in a unified way. The procedure investigated here permits to recover almost all known solutions, and allows one to derive new solutions as well. In particular, we derive and discuss one novel solution defined in terms of the Lambert function. The solutions are organised in a classification which depends on the choice of a generating function which we have denoted by x(phi) that reflects the underlying thermodynamics of the model. We also analyse and discuss the existence of form-invariance dualities between solutions. A general way of defining the latter in an appropriate fashion for scalar fields is put forward.

Molybdenum Complexes Bearing the Tris(1-pyrazolyl)methanesulfonate Ligand: Synthesis, Characterization and Electrochemical Behaviour

The tris(1-pyrazolyl)methanesulfonate lithium salt Li(Tpms) [Tpms = SO3C(pz)(3)-] reacts with [Mo(CO)(6)] in NCMe heated at reflux to yield Li[Mo(Tpms)(CO)(3)] (1), which, upon crystallization from thf, forms the coordination polymer [Mo(Tpms)(CO)(2)(mu-CO)Li(thf)(2)](n) (2). Reaction of 1 with I-2, HBF4 or AgBF4 yields [Mo(Tpms)I(CO)(3)] (3), (Mo(Tpms)-H(CO)(3)] (5) or (Mo(Tpms)O-2](2)(mu-O) (7), respectively. The high-oxidation-state dinuclear complexes [{Mo(Tpms)O(mu-O)}(2)] (4) and [{Mo(tpms)OCl)(2)](mu-O) (6) are formed upon exposure to air of solutions of 3 and 5, respectively. Compounds 1-7, which appear to be the first tris(pyrazolyl)methanesulfonate complexes of molybdenum to be reported, were characterized by IR, H-1 and C-13 NMR spectroscopy, ESI-MS, elemental analysis, cyclic voltammetry and, in the cases of Li(Tpms) and compounds 2, 4.2CH(3)CN, 6.6CHCl(3) and 7, by X-ray diffraction analyses. Li(Tpms) forms a 1D polymeric structure (i.e., [Li(tpms)](n)} with Tpms as a tetradentate N2O2 chelating ligand that bridges two Li cations with distorted tetrahedral coordination. Compound 2 is a 1D coordination polymer in which Tpms acts as a bridging tetradentate N3O ligand and each Li(thf)(2)(+) moiety is coordinated by one bridging CO ligand and by the sulfonyl group of a contiguous monomeric unit. In 4, 6 and 7, the Tpms ligand is a tridentate chelator either in the NNO (in 4) or in the NNN (in 6 and 7) fashion. Complexes 1, 3 and 5 exhibit, by cyclic voltammetry, a single-electron oxidation at oxidation potential values that indicate that the Tpms ligand has an electron-donor character weaker than that of cyclopentadienyl.

A representação visual dos géneros: uma análise a partir das revistas de segmento masculino

Este e-working paper integra o debate acerca da diversidade de identidades de género a partir da análise das diferentes formas de representação visual dos corpos veiculadas em algumas das revistas de segmento masculino a circular em Portugal (Maxmen, FHM, Men’s Health e GQ). O trabalho começa por apresentar alguns dados indicativos do surgimento e evolução das vendas das revistas de segmento masculino em Portugal, bem como do perfil dos seus leitores. Posteriormente e a partir das imagens que integram estas publicações desenha-se, então, uma reflexão acerca das diferentes concepções de género associadas às revistas. This e-Working Paper incorporates a debate on the diversity of gender identities through the analysis of different ways of visually representing the body carried in some of the male magazines circulating in Portugal (Maxmen, FHM, Men’s Health and GQ). The work begins by presenting some data regarding the emergence and market evolution of male magazines in Portugal, as well as the reader’s profile. Therefore, through the diversity of images that integrate these publications, there will be a discussion on the different conceptions of gender.