Regeneração natural de espécies da Mata Atlǎntica em sub-bosque de Eucalyptus saligna Smith. em uma antiga unidade de produção forestal no Parque das Neblinas, Bertioga, SP

This study aimed at characterizing the potential for natural regeneration of native vegetation in the under-story of an earlier Eucalyptus saligna Smith production stand. The study was carried out at the Parque das Neblinas, Bertioga municipality, SP, in a 45 ha third rotation stand; which had been abandoned 15 years ago for natural regeneration to occur. The sampling was done in 24 plots of 20 × 40 m. The sampled area was of 19,200 m2, with inventory made of 100% of the eucalyptus trees. All regeneration trees with a height ≥ 1.30 m and DBH ≥ 5.0 cm were measured, as well as adult individuals with DBH ≥ 5.0 cm; surveyed in two size classes. 1,417 individuals of E. saligna were measured, with a density of 738,02 individuals/ha and a basal area of 22.69 m2/ha. Among 2,763 natural regeneration individuals, 111 species belonged to 66 genera and 34 botanical families. The species represented 43.7% of the tree richness of neighboring native forest fragments. The total estimated density and the basal area were respectively 1,052.6 individuals/ha and 6.4 m2/ha of autochthonous trees with DBH ≥ 5.0 cm (Class 1); while for regeneration there were 3,864.58 individuals/ha, and 2.76 m2/ha of individuals with a height ≥ 1.30 m and DBH < 5.0 cm (Class 2). Shannon diversity (H') was 2.83 and 3.68, respectively, for Classes 1 and 2, and the corrected species richness for a 1000-individual sample (R1000) were 75.6 and 87.29 (Fisher's a index) for the same classes. The majority of the species (34.84%) was typical from the understory of wet tropical forest and had zoochoric fruit dispersal (67.57%). The results indicate that, under these conditions, a eucalyptus forest is able to provide adequate regeneration niches for native vegetation, and may represent a sink habitat for local populations.

Mutagenicidade de duas espécies do gěnero Alchornea avaliadas através de ensaios com Salmonella microssomo e teste do micronúcleo

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Massa seca e composição bromatológica de quatro espécies de braquiárias semeadas na linha ou a lanço, em consórcio com milho no sistema plantio direto na palha

The objective of this research was to evaluate the dry mass yield and chemical composition of four Brachiaria species in different options for sowing, exclusively or in intercrop with corn crop, under a no-tillage system. The experiment was carried out during the growing seasons of 2006 at FEPE (FE/Unesp, Ilha Solteira Campus) located in Selvíria, Mato Grosso do Sul State, Brazil. The soil of the experimental area was classified as distroferric Red Latosol (Oxisol). The experimental design was in randomized blocks, in a factorial scheme (4 × 4), with five replications. The treatments consisted of four Brachiaria species (Brachiaria brizantha cv. Marandu, Brachiaria decumbens, Brachiaria ruziziensis and Mulato II grass) grown in rows and spread on total area, exclusively or intercropped simultaneously with corn crop sowing. The study evaluated the dry mass yield and total digestible nutrients, crude protein, ash, neutral detergent fiber, acid detergent fiber, hemicelluloses, cellulose and lignin content of forage. The spread on total area intercrop of forages with corn crop proved to be viable by presenting similar dry mass yield to exclusive sowing arrangements, conversely to what happened with intercrop in row of corn crop, which decreased such yield. Brachiaria ruziziensis showed superior chemical composition and the intercrops increased energy and crude protein contents, and decreased cell wall components.

Determinação da massa seca, teor de nutrientes e cobertura do solo de espécies semeadas no outono-inverno

Culture options for the autumn-winter season are a major problem for production systems under low rainfall. The aim of this study was to evaluate the effect of the sowing season on dry matter yield, nutrient content of the shoot and soil covering percentage of coverage plants on the soil on the intercrop: grain sorghum, rattlepods, pearl millet brachiaria grass and an area with weeds (fallow). The experiment was conducted under field conditions on an Oxisol (Haplustox), clay texture, in Selvíria, Mato Grosso do Sul, Brazil. The experimental design was a randomized block design with eight replications, two sowing seasons and five coverage treatments. When the sorghum culture of the first sowing season reached the harvest stage, the dry matter yield on the other treatments was evaluated. Results showed that sowing in March results in higher dry mass yield and higher soil coverage percentage in the studied species. In the first sowing season, Brachiaria brizantha presents higher dry matter yield and macronutrients extraction.

Morfologia de sementes e de estádios iniciais de plântulas de espécies de Bromeliaceae da Amazônia

Seeds of Aechmea bromeliifolia, A. castelnavii (Bromelioideae); Dyckia duckei, D. racemosa (Pitcairnioideae) and Tillandsia adpressiflora (Tillandsioideae) were collected in the Amazon regions (Mato Grosso) and studied to describe morphological characterization and post-seminal development, which can be taxonomically useful, and to assess percent germination. All the species have epigeous germination and produce cryptocotyledonary plantlets. Seeds have no dormancy and percent germination is high (over 86%), which facilitates the production of seedlings and conservation studies. Exclusive characteristics of the genera include: the seed coat of Aechmea (Bromelioideae) has mucilage that prevents desiccation; whereas that of Dyckia (Pitcairnioideae) has membranaceous wings and that of Tillandsia (Tillandsioideae) has feathery appendages, both of which make dispersal easier and establish the epiphytic habit. Initial post-seminal development of Aechmea (Bromelioideae) and Dyckia (Pitcairnioideae) is marked by the emergence of primary roots, interpreted as a basal character, whereas that of Tillandsia adpressiflora (Tillandsioideae) is marked by the emergence of the cotyledon, interpreted as a derived character. Dyckia and Tillandsia have a small tank only in the seedling phase while the contrary occurs in Aechmea.

Efeito do tratamento químico na sanidade de sementes de espécies florestais

This study evaluated the effect of different fungicide and sodium hypochlorite treatments in reducing the incidence of fungi on seeds of native species of the Atlantic rainforest. The experiment was done using the completely randomized design in a 5×5 factorial with five levels of factor A (forest species) and five levels of factor B (chemical treatment and surface disinfection with sodium hypochlorite) with four replications. The fungal genera Fusarium, Alternaria, Aspergillus and Penicillium were identified. The species 'Jacarandáda- Bahia' and 'Ipê-roxo' had lower fungal incidence, 1.0% and 1.3%, respectively. Except for 'Angico Vermelho', the fungicide pencycurom and sodium hypochlorite were not significantly different from the control in the species evaluated. Seed treatment with fungicides 'Captan' and 'Tiram' yielded satisfactory results in reducing the incidence of fungal contamination in forest species seeds.

Soroprevalência de anticorpos contra Leishmania infantum e outras espécies de protozoários e rickettsia em cães

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Identificação de espécies e genótipos de Cryptosporidium em bovinos leiteiros no Brasil

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Efeito de três espécies de gramíneas forrageiras sobre a estrutura da pastagem e distribuição vertical de larvas infectantes de nematódeos gastrintestinais de ovinos

The knowledge of interaction between infective larvae setting and the type of grass is important to epidemiological studies and the control of gastrointestinal nematodes in sheep. The objective of this work was to evaluate the effects of three species of forage grasses on pasture characteristics and the vertical distribution of infective larvae (L3) of gastrointestinal nematodes of woolless sheep on the grasses during the rainy season. Sixty non-periparturients ewes were used, naturally infected, equally distributed on 2ha paddocks sowed with Tanzania, star, and gamba grasses, managed under continuous grazing system, from October 2003 to March 2004, at Santa Bárbara farm, Barreiras-Bahia-Brazil. Data of three samples between December 2003 and March 2004 were analyzed by SAS, using split-plot design, with 10 replications. Infective larvae of Haemonchus spp. and Trichostrongylus spp. were observed on forage in all stratus of vertical layer of the grasses without a defined pattern. Pastures with different characteristics under continuous grazing system had good conditions for developing infective larvae of sheep.

Descrição cariotípica de possíveis novas espécies do complexo Hypostomus ancistroides e outros Hypostominae

Cytogenetic analyses were performed in four species of the Hypostominae subfamily, three from Hypostomus (Hypostomini) genus and Rhinelepis aspera (Rhinelepini). Three populations of Hypostomus ancistroides were analyzed, which had 2n=68 chromosomes, but presented different karyotype formulas. Hypostomus regani and H. strigaticeps, both from Ivaí river, showed 2n=72 chromosomes with two distinct cytotypes. In turn, R. aspera of the upper Paraná river basin presented 2n=54 chromosome. Multiple Nucleolar Organizer Regions (NORs) have been evidenced by silver nitrate staining in species of Hypostomus and single NOR in R. aspera. The observed variation in the chromosome number and the marked variability in karyotype formulas and NORs reveal a certain amount of karyotype variation in the genus Hypostomus suggesting the probable existence of cryptic species with independent chromosome traits. Therefore, our data can be of great value in discriminating species and understanding their chromosomal evolution.

Tolerância de Espécies de Mucuna a Herbicidas Utilizados na Cultura da Cana-de-Açúcar

The emergence of Mucuna species in sugarcane plantations, even after herbicide spraying for weed management, allowed us to elaborate the hypothesis that these plants are tolerant to the herbicides commonly used in the culture. Thus, to prove this hypothesis, this work was carried out to study the tolerance of Mucuna aterrima, Mucuna cinerea and Mucuna deeringiana to application of herbicides with different mechanisms of action in pre- and postemergence. The experiment was arranged in a randomized block design with 9 treatments and five replications in a factorial 3 × 3, plus controls. At pre-emergence, the first factor was constituted by the herbicides sulfentrazone (800 g ha-1), imazapic (245 g ha-1), and amicarbazone (1,400 g ha-1), and the second factor by the Mucuna species, besides the additional control for each species studied. At post- emergence, the herbicides tested were clomazone (1,100 g ha-1), ametryn+trifloxysulfuronsodium (1,463 + 37 g ha-1), and 2,4-D (1,209 g ha-1). In the chemical management at pre-emergence, the species were sensitive to amicarbazone, followed by sulfentrazone, and tolerant to imazapic. At post-emergence, all species were sensitive to ametryn + trifloxysulfuron-sodium and 2,4-D, but tolerant to clomazone.

Identificação de espécies fúngicas nas onicomicoses do idoso institucionalizado

BACKGROUND: Superficial fungal infections are caused by dermatophytes, yeasts or filamentous fungi. They are correlated to the etiologic agent, the level of integrity of the host immune response, the site of the lesion and also the injured tissue. OBJECTIVE: The purpose of this study is to isolate and to identify onychomycosis agents in institutionalized elderly (60 years old +). METHODS: The identification of the fungi relied upon the com-bined results of mycological examination, culture isolation and micro cultures observation under light microscopy from nail and interdigital scales, which were collected from 35 elderly with a clinical suspicion of onychomycosis and a control group (9 elderly with healthy interdigital space and nails). Both groups were insti-tutionalized in two nursing homes in Sao Bernardo do Campo, SP, Brazil. RESULTS: The nail scrapings showed 51.40% positivity. Of these, dermatophytes were found in 44.40% isolates, 27.78% identified as Trichophyton rubrum and 5.56% each as Trichophyton tonsurans, Trichophyton mentagrophytes and Microsporum gypseum. The second more conspicuous group showed 38.89% yeasts: 16.67% Candida guilliermondii, 11.11% Candida parapsilosis, 5.56% Candida glabrata, and 5.56% Trichosporon asahii. A third group displayed 16.70% filamen-tous fungi, like Fusarium sp, Aspergillus sp and Neoscytalidium sp (5.56% each). The interdigital scrapings pre-sented a positivity rate of 14.29%. The agents were coincident with the fungi that caused the onychomycosis. In the control group, Candida guilliermondii was found at interdigital space in one person. CONCLUSION: Employing a combination of those identification methods, we found no difference between the etiology of the institutional-ized elderly onychomycosis from that reported in the literature for the general population. © 2013 by Anais Brasileiros de Dermatologia.

Densidade e época de semeadura de duas espécies de Brachiaria sobre a cultura da soja

The aim of this study was to evaluate the growth and yield of soybean cultivar M-8766 in consortium with Brachiaria brizantha. BRS Piata and Brachiaria ruziziensis at different densities and sowing dates. The experimental design was randomized blocks with treatments arranged in a factorial 2 × 2 × 3 with four replications. Used as factors grass species (Brachiaria brizantha Piata and Brachiaria ruziziensis BRS) intercropped with soybean M-8766, sowing dates (12 and 24 days after soybean emergence) and three seeding rates (0, 5, 10 kg ha-1 of seed). At 71 days after soybean emergence were evaluated plant height, stem diameter, dry mass of leaves, stems and shoots, and 4 months after sowing determined the weight of 100 grains and soybean yield. The results showed that when seeded at a density of 10 kg ha-1 at 12 and 24 DAE soy, Brachiaria brizantha. BRS Piata caused reduction in yield in the order of 6.71% and 3.03% respectively, while the Brachiaria ruziziensis was one that caused a greater reduction in productivity in the order of 13.42 and 16.23%, respectively, of these values expression when considering the price of soybean sack. B. ruziziensis expressed less competitive with soybean. However, the large biomass production of this grass provides deployment system till the next harvest.

Investigação sorológica de espécies de Ehrlichia em cães, equinos e humanos de um assentamento rural do sul do Brasil

The aims of this study were to determine the seroprevalence of Ehrlichia spp. and risk factors for exposure in a restricted population of dogs, horses, and humans highly exposed to tick bites in a Brazilian rural settlement using a commercial ELISA rapid test and two indirect immunofluorescent assays (IFA) with E. canis and E. chaffeensis crude antigens. Serum samples from 132 dogs, 16 horses and 100 humans were used. Fifty-six out of 132 (42.4%) dogs were seropositive for E. canis. Dogs > one year were more likely to be seropositive for E. canis than dogs ≤ one year (p = 0.0051). Ten/16 (62.5%) and 8/16 (50%) horses were seropositive by the commercial ELISA and IFA, respectively. Five out of 100 (5%) humans were seropositive for E. canis and E. chaffeensis. Rhipicephalus sanguineus (n = 291, 97.98%) on dogs and Amblyomma cajennense (n = 25, 96.15%) on horses were the most common ticks found. In conclusion, anti-Ehrlichia spp. antibodies were found in horses; however, the lack of a molecular characterization precludes any conclusion regarding the agent involved. Additionally, the higher seroprevalence of E. canis in dogs and the evidence of anti-Ehrlichia spp. antibodies in humans suggest that human cases of ehrlichiosis in Brazil might be caused by E. canis, or other closely related species.

Avaliação qualitativa e quantitativa na deposição de calda de pulverização em quatro espécies do gênero commelina

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)