Occurrence and molecular characterization of cryptococcosis in dogs and cats in Mato Grosso, Brazil

Cryptococcosis is an infection that affects humans and animals, the etiology is attributed to Cryptococcus neoformans variety neoformans, C. neoformans var. grubii and Cryptococcus gattii. The infection is common in dogs and cats, causing respiratory, neurological, cutaneous and ocular infections. Aiming to better understand the epidemiology of cryptococcosis in animals in the region, this paper describe the occurrence and characterization of the Cryptococcus species involved in this illness in pet animals at Mato Grosso State, Brazil. Clinical samples of four cases, two in cats and two dogs, were submitted for pathological, microbiological and molecular analysis. Microscopically, in three cases, tissue sections stained with hematoxylin and eosin had absence to severe granulomatous reaction composed by histiocytes, multinucleated cells and lymphocytes infiltration. In one case, citological imprint analysis showed similar inflammatory mainly mononuclear and lymphocyte cells infiltration. All cases had variable amounts of intracellular and extracellular fungal structures compatible with Cryptococcus sp. on Periodic Acid-Schiff (PAS) stain. All clinical samples were positive for culture on Sabouraud Dextrose Agar (SDA) and morphologically classified as Cryptococcus sp. The isolates were PCR positive for C. gatti, being confirmed by sequencing technique. The findings characterize the molecular species involved in animal infections in the region, and may contribute to future studies of the epidemiology of C. gattii.

Efeitos alelopaticos de alguns extratos vegetais na germinação do tomateiro (Lycopersicon esculentum Mill. cv. Santa Cruz)

O presente trabalho teve por finalidade avaliar os possíveis efeitos alelopáticos de três plantas daninhas (Cyperus rotundus, Sorghum halepense e Cynodon dactylon), uma leguminosa (Canavalia ensiformis) e colza (Brassica napus), na germinação das sementes do tomateiro (Lycopersicon esculentum cv. Santa Cruz) utilizado como indicador. Tubérculos de tiririca, rizomas de capim massambará, o sistema radicular de grama seda, além de folhas e raízes de feijão-de-porco e colza, foram homogeneizados em solução aquosa, submetidos a filtração e centrifugação. A aplicação do sobrenadante no substrato de germinação das sementes de tomateiro mostrou afetar o processo germinativo e o desenvolvimento da radícula e do hipocótilo. C. rotundus, S. halepense e raízes de C. ensiformis possuem substâncias altamente inibitórias à germinação do tomateiro 'Santa Cruz'', sendo que a planta indicadora também mostrou-se sensível às substâncias presentes nas folhas de B. napus e em C. dactylon.

Plasma cytokine response, lipid peroxidation and NF-κB activation in skeletal muscle following maximum progressive swimming

Our objective was to determine lipid peroxidation and nuclear factor-κB (NF-κB) activation in skeletal muscle and the plasma cytokine profile following maximum progressive swimming. Adult male Swiss mice (N = 15) adapted to the aquatic environment were randomly divided into three groups: immediately after exercise (EX1), 3 h after exercise (EX2) and control. Animals from the exercising groups swam until exhaustion, with an initial workload of 2% of body mass attached to the tail. Control mice did not perform any exercise but were kept immersed in water for 20 min. Maximum swimming led to reactive oxygen species (ROS) generation in skeletal muscle, as indicated by increased thiobarbituric acid reactive species (TBARS) levels (4062.67 ±1487.10 vs 19,072.48 ± 8738.16 nmol malondialdehyde (MDA)/mg protein, control vs EX1). Exercise also promoted NF-κB activation in soleus muscle. Cytokine secretion following exercise was marked by increased plasma interleukin-6 (IL-6) levels 3 h post-exercise (P < 0.05). Interleukin-10 (IL-10) levels were reduced following exercise and remained reduced 3 h post-exercise (P < 0.05). Plasma levels of other cytokines investigated, monocyte chemotactic protein-1 (MCP-1), tumor necrosis factor-alpha (TNF-α), interferon-gamma (IFN-γ) and interleukin-12 (IL-12), were not altered by exercise. The present findings showed that maximum swimming, as well as other exercise models, led to lipid peroxidation and NF-κB activation in skeletal muscle and increased plasma IL-6 levels. The plasma cytokine response was also marked by reduced IL-10 levels. These results were attributed to exercise type and intensity.

La industria en el Area Metropolitana de Monterrey proposición de un modelo urbanístico de simulación Eduardo Sousa González.

Tesis (Maestría en Ciencias para la Planificación de Asentamientos Humanos) U.A.N.L. Facultad de Arquitectura.

Estrategias y técnicas para optimizar el crédito y la cobranza [por] Javier Humberto Guajardo Cruz

Tesis (Maestría Contaduría Pública) U.A.N.L.

Obtención y caracterización de gel de sílice para cromatografía [por] Cruz Imelda Cruz Páez

Tesis (Maestría en Ciencias, con especialidad en Química Analítica) U.A.N.L.

Redes de apoyo social y prácticas de salud del adolescente [por] Patricia Cruz Bello

Tesis (Maestría en Enfermería con Especialidad en Salud Comunitaria) U.A.N.L.

Explotación futura del acuífero del Valle de Caborca, Sonora [por] Arturo Ojeda de la Cruz

Tesis (Maestría en Ciencias con Especialidad en Hidrología Subterránea) U.A.N.L.

Ecuaciones de volumen y funciones de ahusamiento para Pinus durangensis Mart. y Pinus teocote Schl. et Cham. del ejido Vencedores, San Dimas, Durango, México [por] Cruz Contreras Aviña

Tesis (Maestría en Ciencias Forestales) U.A.N.L.

Patrones de discriminación en ingreso por género según estrato socioeconómico estudio para el área metropolitana de Monterrey, 1993 [por] Cruz Evelia González Juanes

Tesis (Maestría en Economía con Especialidad en Economía Industrial) U.A.N.L.

Propuesta didáctica la aplicación de procedimientos heurísticos y situaciones problémicas en la resolución de problemas de matemáticas I [por] Francisco Balderas Cruz

Tesis (Maestría en la Enseñanza de la Ciencia con Especialidad en Matemáticas) U.A.N.L.

Activación mecánica de arcillas [por] Javier Cruz Cantú

Tesis (Maestría en Ciencias con Especialidad en Ingeniería Cerámica) U.A.N.L.

Originalidad e hibridación en Amor es más laberinto. Comedia escrita por Sor Juana Inés de la Cruz y Juan de Guevara en 1689.

Tesis (Maestría en Ciencias con Especialidad en Lengua y en Literatura) UANL, 2012.