USDAHL-74 revised model of watershed hydrology : a United States contribution to the international hydrological decade /

Literature cited: p. 29-30.

Poland's contribution to the world's civilization,

Mode of access: Internet.

Discoveries in Chinese; or, The symbolism of the primitive characters of the Chinese system of writing. As a contribution to philology and ethnology and a practical aid in the acquisition of the Chinese language,

Mode of access: Internet.

The heart as a power-chamber; a contribution to cardio-dynamics,

Mode of access: Internet.

Human rights : State Department's commitment to accurate reporting has increased : report to the chairman, Legislation and National Security Subcommittee, Committee on Government Operations, House of Representatives /

"B-240397"--P. [1]

Veterans' employment and training : services provided by Labor Department programs : report to the Chairman, Subcommittee on Benefits, Committee on Veterans' Affairs, House of Representatives /

"B-275189"--P. 1.

Robert Grosseteste, Bishop of Lincoln; a contribution to the religious, political and intellectual history of the thirteenth century.

Includes bibliography.

Paulinism; a contribution to the history of primitive Christian theology. /

v. 1. Exposition of Paul's doctrine.--v. 2. The history of Paulinism in the primitive church.

Hours with the mystics : a contribution to the history of religious opinion /

Includes bibliographical references and index.

Married love : a new contribution to the solution of sex difficulties /

Mode of access: Internet.

A contribution to the flora of Australia,

Mode of access: Internet.

Paul, the apostle of Jesus Christ : his life and work, his epistles and his doctrine : a contribution to a critical history of primitive Christianity /

Translation of: Paulus, der Apostel Jesu Christi.

A contribution to the pharmacognosy of official strophanthus seed /

"Read before the British Pharmaceutical Conference in London, July, 1900."

beta 2 subunit contribution to 4/7 alpha-conotoxin binding to the nicotinic acetylcholine receptor

The structures of acetylcholine-binding protein ( AChBP) and nicotinic acetylcholine receptor ( nAChR) homology models have been used to interpret data from mutagenesis experiments at the nAChR. However, little is known about AChBP-derived structures as predictive tools. Molecular surface analysis of nAChR models has revealed a conserved cleft as the likely binding site for the 4/7 alpha-conotoxins. Here, we used an alpha 3 beta 2 model to identify beta 2 subunit residues in this cleft and investigated their influence on the binding of alpha-conotoxins MII, PnIA, and GID to the alpha 3 beta 2 nAChR by two-electrode voltage clamp analysis. Although a beta 2-L119Q mutation strongly reduced the affinity of all three alpha-conotoxins, beta 2-F117A, beta 2-V109A, and beta 2-V109G mutations selectively enhanced the binding of MII and GID. An increased activity of alpha-conotoxins GID and MII was also observed when the beta 2-F117A mutant was combined with the alpha 4 instead of the alpha 3 subunit. Investigation of A10L-PnIA indicated that high affinity binding to beta 2-F117A, beta 2-V109A, and beta 2-V109G mutants was conferred by amino acids with a long side chain in position 10 (PnIA numbering). Docking simulations of 4/7 alpha-conotoxin binding to the alpha 3 beta 2 model supported a direct interaction between mutated nAChR residues and alpha-conotoxin residues 6, 7, and 10. Taken together, these data provide evidence that the beta subunit contributes to alpha-conotoxin binding and selectivity and demonstrate that a small cleft leading to the agonist binding site is targeted by alpha-conotoxins to block the nAChR.

ACcESS: Australia's contribution to the iSERVO Institute's development

The new Australian Computational Earth Systems Simulator research facility provides a virtual laboratory for studying the solid earth and its complex system behavior. The facility's capabilities complement those developed by overseas groups, thereby creating the infrastructure for an international computational solid earth research virtual observatory.