Estratégia operacional de sistema formado por reator não compartimentado com setores com aeração/sem aeração precedido por reator anaeróbio

A opção por sistemas biológicos prevalece para o tratamento do esgoto sanitário. Nas décadas recentes, sistemas que possuem regiões e/ou zonas anaeróbia, anóxica e aeróbia têm-se mostrado como alternativas atraentes para remoção simultânea de matéria orgânica, nitrogênio e fósforo. No entanto, os aspectos operacionais ainda merecem ser objeto de estudo para alcançar desempenho otimizado. Nesse cenário, com intuito de comparar alternativas para a operação das unidades de tratamento de esgoto, o presente trabalho propôs-se a estudar estratégias operacionais associadas ao monitoramento, em tempo real, sem adição de fonte externa de carbono, para um reator aerado não compartimentado com crescimento suspenso e fluxo contínuo precedido de reator anaeróbio. O sistema experimental, em escala de bancada, era constituído de um reator anaeróbio, com volume útil de 43,54 L, e reator aerado, com volume útil de 68,07 L; sendo que este era formado por sete setores, em série, sem separação física. O estudo foi dividido em duas etapas: I - estudo da variação dos volumes da região aerada e da não aerada; II - estudo da aeração intermitente com ciclo de aeração/agitação pré-fixado e controlado em tempo real por sistema informatizado. Em todas as Etapas do estudo ocorreu elevada remoção de DBO e conversão de NTK para nitrato, contudo não se conseguiu obter desnitrificação em nível desejado. O uso de reatores com setores sequenciais sem divisão física (Etapa I) dificultou a obtenção de regiões distintas predominantemente anóxica e aeróbia, comprometendo a remoção de nitrogênio (principalmente a desnitrificação). A maior eficiência média de remoção de nitrogênio alcançada no reator aerado foi de 35,6% (Etapa II), quando o reator era operado com aeração intermitente sendo o ciclo de aeração/agitação controlado em tempo real. A estratégia de operação com aeração intermitente, estudada na Etapa II, favoreceu a remoção de nitrogênio. A aeração intermitente demonstrou ser uma opção promissora comparada à aeração contínua em setores específicos do reator. O controle automatizado e informatizado em tempo real dos ciclos de aeração/agitação pode ser aplicado no aperfeiçoamento da operação dos sistemas de tratamento de esgoto sanitário.

Produção de biohidrogênio a partir de soro de leite em AnSBBR com recirculação da fase líquida

O presente estudo investigou a aplicação de dois tipos de AnSBBR (reatores anaeróbio com biofilme e operados em batelada e batelada alimentada sequenciais: com recirculação da fase líquida e com agitação) para produção de biohidrogênio tratando água residuária sintética (a base de soro de leite e lactose, respectivamente). O AnSBBR com recirculação da fase líquida, que foi o estudo principal do presente trabalho, apresentou problemas na produção de hidrogênio utilizando soro de leite como substrato. Algumas alternativas, como adaptação da biomassa com substratos puros de degradação mais fácil, controle do pH em valores muito baixos e diferentes formas de inoculação foram testadas, entretanto, sem obtenção de sucesso. A solução do problema foi obtida ao refrigerar o meio de alimentação a 4ºC para evitar a fermentação no frasco de armazenamento, retirar a ureia e a suplementação de nutrientes, e realizar lavagens periódicas do material suporte para retirada de parte da biomassa. Dessa forma eliminaram-se indícios de produção de H2S por possível ação de bactérias redutoras de sulfato (BRS) e atingiu-se uma produção estável de hidrogênio sem, entretanto, eliminar completamento o metano, que foi produzido em baixas concentrações. Depois de atingida a estabilidade, investigou-se a influência da concentração afluente de substrato, do tempo de enchimento e da temperatura na produção de biohidrogênio no AnSBBR com recirculação da fase líquida tratando soro de leite. O estudo da concentração afluente apresentou um ponto ótimo para a concentração de 5400 mgDQO.L-1, atingindo valores de 0,80 mol H2.mol-1 lactose e de 660 mL H2.L-1.d-1. O estudo do tempo de enchimento apresentou resultados similares para as condições analisadas. Com relação à temperatura, os melhores resultados foram obtidos com a temperatura mais baixa testada de 15ºC (1,12 mol H2.mol lactose-1 e 1080 mL H2.L-1.d-1), sendo que na temperatura mais alta testada (45°C) não ocorreu produção de hidrogênio. Para o AnSBBR com agitação mecânica, que foi um estudado complementar realizado pelo fato da lactose ser o principal complemento do soro de leite, o desempenho do biorreator foi avaliado de acordo com influência conjunta do tempo de ciclo (tC – 2, 3 e 4 h), da concentração afluente (CSTA – 3600-5400 mgDQO.L-1) e da carga orgânica volumétrica aplicada (COAV – 9,3, 12,3, 13,9, 18,5 e 27,8 mgDQO.L-1.d-1). Foram obtidos excelentes resultados: consumos de carboidratos (lactose), com valores médios sempre acima de 90% e uma produção estável de biohidrogênio em todas as condições estudadas, com metano em baixas concentrações apenas na condição de maior COAV. A diminuição do tC apresentou tendência clara de melhora sobre o RMCRC,n (rendimento molar entre hidrogênio produzido e carboidrato removido) apenas para as condições com menor concentração CSTA, havendo uma relação direta entre CSTA, e RMCRC,n em todos os valores de tC, exceto para o tempo de ciclo de 3 h, exatamente onde ocorreu produção de metano. O melhor valor de RMCRC,n obtido na operação com lactose (1,65 mol H2.mol Carboidrato-1) foi superior aos obtidos em outros trabalhos utilizando a mesma configuração de reator e sacarose como substrato. As análises filogenéticas mostraram que a maioria dos clones analisados foi semelhante à Clostridium. Além destes, clones filogeneticamente semelhantes com a Família Lactobacilaceae, especificamente Lactobacillus rhamnosus foram observados em menor porcentagem no reator, assim como clones com sequências semelhantes a Acetobacter indonesiensis.

Análise dos parâmetros que interferem no metabolismo da microbiota anaeróbia e anóxica na remoção de bifenilas policloradas

A elevada concentração de cloro das bifenilas policloradas provoca alta toxicidade do composto, o qual dificulta sua biodegradação. A contaminação de PCB no Brasil foi confirmada em estudo realizado na Bahia de Santos-São Vicente (São Paulo), o qual revelou a necessidade de um plano de ação para o controle e remoção de PCB no Brasil. Pretendeu-se assim, na realização da presente pesquisa, verificar quatro hipóteses: (1) A técnica de Microextração em fase sólida é uma metodologia eficaz para avaliação de bifenilas policloradas de amostras de reatores; (2) A condição fermentativa-metanogênica abriga comunidade resistente ao PCB, e removê-lo; (3) A condição desnitrificante abriga comunidade resistente ao PCB, e removê-lo e (4) A remoção de PCB, bem como, a composição microbiana é distinta em cada condição metabólica. Para tanto, reatores em batelada foram montados separadamente com biomassa anaeróbia proveniente de reator UASB usado no tratamento de água residuária de avicultura e biomassa de sistemas de lodos ativados de tratamento de esgoto sanitário. Os reatores operados em condição mesófila foram alimentados com meio sintético, co-substratos, sendo etanol (457 mg.L-1) e formiato de sódio (680 mg.L-1) para os reatores anaeróbios, e somente etanol (598 mg.L-1) para os reatores anóxicos, além de PCB padrão Sigma (congêneres PCBs 10, 28, 52, 153, 138 e 180) em diferentes concentrações, dependendo do objetivo do ensaio. A aplicação do método de extração por SPME com análise em cromatógrafo gasoso com detector por captura de elétrons foi adequada para a determinação dos seis congêneres de PCB. Obteve-se ampla faixa de linearidade, seletividade frente aos vários interferentes, além da robustez do método, utilidade e confiabilidade na identificação e quantificação específica dos seis congêneres de PCB. A Hipótese 1 foi aceita; ou seja, por meio da aplicação da metodologia SPME foi possível quantificar os PCB nos reatores em batelada. Apesar de ter sido comprovada a inibição metanogênica na presença de PCB, com IC50 de 0,03 mg.L

Utilização de metanol, etanol e metano como doadores de elétrons para a desnitrificação

Esta tese apresenta e discute os dados obtidos a partir de trabalho experimental projetado para avaliar comparativamente o desempenho de reatores desnitrificantes em batelada, tendo etanol, metanol e gás metano como doadores de elétrons. Os experimentos foram realizados em reatores em escala de bancada. Os ensaios com gás metano objetivaram verificar a efetividade deste sub-produto de reatores anaeróbios em substituir os doadores exógenos de elétrons comumente utilizados, tais como metanol e etanol. Para alcançar o objetivo principal deste trabalho, os parâmetros cinéticos de desnitrificação, para os doadores de elétrons ensaiados, foram determinados nas diferentes condições operacionais. Além disso, as alterações ocorridas na população microbiana, ao longo do período experimental, foram avaliadas em relação à diversidade microbiana, por meio de análises microscópicas (óptica, de fluorescência e eletrônica de varredura) e da técnica de Biologia Molecular de PCR/DGGE. A completa desnitrificação foi alcançada para todos os compostos testados, e o etanol foi o doador de elétrons mais eficiente para a desnitrificação. A melhor razão carbono-nitrogênio para a desnitrificação foi igual a 1,0. Contudo, este parâmetro foi encontrado ser inadequado para utilização no processo de desnitrificação, uma vez que não expressa a capacidade real do composto usado em doar elétrons. A desnitrificação com metano ocorreu tanto na presença como na ausência de oxigênio, embora a baixas velocidades quando comparado com os outros compostos. No entanto, a configuração do reator utilizado neste estudo não foi adequada para promover a efetiva dissolução do gás metano na fase líquida. Por essa razão, sugere-se o desenvolvimento de configurações de reatores apropriadas para minimizar as resistências à transferência de massa da fase gasosa para a líquida e também desta para a biomassa.

Water reduction in waste-activated sludge by resettling and filtration in batch. Phase (1): pilot-scale experiments to optimize performance

This article describes an effective procedure for reducing the water content of excess sludge production from a wastewater treatment plant by increasing its concentration and, as a consequence, minimizing the volume of sludge to be managed. It consists of a pre-dewatering sludge process, which is used as a preliminary step or alternative to the thickening. It is made up of two discontinuous sequential stages: the first is resettling and the second, filtration through a porous medium. The process is strictly physical, without any chemical additives or electromechanical equipment intervening. The experiment was carried out in a pilot-scale system, consisting of a column of sedimentation that incorporates a filter medium. Different sludge heights were tested over the filter to verify the influence of hydrostatic pressure on the various final concentrations of each stage. The results show that the initial sludge concentration may increase by more than 570% by the end of the process with the final volume of sludge being reduced in similar proportions and hydrostatic pressure having a limited effect on this final concentration. Moreover, the value of the hydrostatic pressure at which critical specific cake resistance is reached is established.

The influence of substrate kinetics on the microbial community structure in granular anaerobic biomass

The development of a strong, active granular sludge bed is necessary for optimal operation of upflow anaerobic sludge blanket reactors. The microbial and mechanical structure of the granules may have a strong influence on desirable properties such as growth rate, settling velocity and shear strength. Theories have been proposed for granule microbial structure based on the relative kinetics of substrate degradation, but contradict some observations from both modelling and microscopic studies. In this paper, the structures of four granule types were examined from full-scale UASB reactors, treating wastewater from a cannery, a slaughterhouse, and two breweries. Microbial structure was determined using fluorescence in situ hybridisation probing with 16S rRNA-directed oligonucleotide probes, and superficial structure and microbial density (volume occupied by cells and microbial debris) assessed using scanning electron microscopy (SEM), and transmission electron microscopy (TEM). The granules were also modelled using a distributed parameter biofilm model, with a previously published biochemical model structure, biofilm modelling approach, and model parameters. The model results reflected the trophic structures observed, indicating that the structures were possibly determined by kinetics. Of particular interest were results from simulations of the protein grown granules, which were predicted to have slow growth rates, low microbial density, and no trophic layers, the last two of which were reflected by microscopic observations. The primary cause of this structure, as assessed by modelling, was the particulate nature of the wastewater, and the slow rate of particulate hydrolysis, rather than the presence of proteins in the wastewater. Because solids hydrolysis was rate limiting, soluble substrate concentrations were very low (below Monod half saturation concentration), which caused low growth rates. (C) 2003 Elsevier Ltd. All rights reserved.

Effects of acetate and propionate on the performance of a photosynthetic biofilm reactor for sulfide removal

The effects of acetate and propionate on the performance of a recently proposed and characterized photosynthetic biological sulfide removal system have been investigated with a view to predicting this concept's suitability for removing sulfide from wastewater undergoing or having undergone anaerobic treatment. The concept relies on substratum-irradiated biofilms dominated by green sulfur bacteria (GSB), which are supplied with radiant energy in the band 720 - 780 nm. A model reactor was fed for 7 months with a synthetic wastewater free of volatile fatty acids (VFAs), after which time intermittent dosing of the wastewater with acetate or propionate was begun. Such dosing suppressed the areal net sulfide removal rate by similar to50%, and caused the principal net product of sulfide removal to switch from sulfate to elemental-S. Similarly suppressed values of this rate were observed when the wastewater was dosed continuously with acetate, and this rate was not significantly affected by changes in the concentration of ammonia-N in the feed. The main net product of sulfide removal was again elemental-S, which was scarcely released into the liquid, however. Sulfate reduction and sulfur reduction were observed when the light supply was interrupted and were inferred to be occurring within the irradiated biofilm. A preexisting conceptual model of the biofilm was augmented with both of these reductive processes, and this augmented model was shown to account for most of the observed effects of VFA dosing. The implications of these findings for the practicality of the technology are considered. (C) 2004 Wiley Periodicals, Inc.

Anaerobic metabolism of propionate by polyphosphate-accumulating organisms in enhanced biological phosphorus removal systems

Propionate, a carbon substrate abundant in many prefermenters, has been shown in several previous studies to be a more favorable substrate than acetate for enhanced biological phosphorus removal (EBPR). The anaerobic metabolism of propionate by polyphosphate accumulating organisms (PAOs) is studied in this paper. A metabolic model is proposed to characterize the anaerobic biochemical transformations of propionate uptake by PAOs. The model is demonstrated to predict very well the experimental data from a PAO culture enriched in a laboratory-scale reactor with propionate as the sole carbon source. Quantitative fluorescence in-situ hybridization (FISH) analysis shows that Candidatus Accumulibacter phosphatis, the only identified PAO to date, constitute 63% of the bacterial population in this culture. Unlike the anaerobic metabolism of acetate by PAOs, which induces mainly poly-beta-hydroxybutyrate (PHB) production, the major fractions of poly-beta-hydroxyalkanoate (PHA) produced with propionate as the carbon source are poly-beta-hydroxyvalerate (PHV) and poly-beta-hydroxy-2-methylvalerate (PH2MV). PHA formation correlates very well with a selective (or nonrandom) condensation of acetyl-CoA and propionyl-CoA molecules. The maximum specific propionate uptake rate by PAOs found in this study is 0.18 C-mol/C-mol-biomass h, which is very similar to the maximum specific acetate uptake rate reported in literature. The energy required for transporting 1 carbon-mole of propionate across the PAO cell membrane is also determined to be similar to the transportation of 1 carbon-mole of acetate. Furthermore, the experimental results suggest that PAOs possess a similar preference toward acetate and propionate uptake on a carbon-mole basis. (c) 2005 Wiley Periodicals, Inc.

Competition between polyphosphate and glycogen accumulating organisms in enhanced biological phosphorus removal systems with acetate and propionate as carbon sources

Enhanced biological phosphorus removal (EBPR) is a widely used process for achieving phosphorus removal from wastewater. A potential reason for EBPR failure is the undesirable growth of glycogen accumulating organisms (GAOs), which can compete for carbon sources with the bacterial group responsible for phosphorus removal from wastewater: the polyphosphate accumulating organisms (PAOs). This study investigates the impact of carbon source on EBPR performance and the competition between PAOs and GAOs. Two sequencing batch reactors (SBRs) were operated during a 4-6 month period and fed with a media containing acetate or propionate, respectively, as the sole carbon source. It was found that the acetate fed SBR rarely achieved a high level of phosphorus removal, and that a large portion of the microbial community was comprised of Candidatus Competibacter phosphatis, a known GAO. The propionate fed SBR, however, achieved stable phosphorus removal throughout the study, apart from one brief disturbance. The bacterial community of the propionate fed SBR was dominated by Candidatus Accumulibacter phosphatis, a known PAO, and did not contain Competibacter In a separate experiment, another SBR was seeded with a mixture of PAOs and a group of alphaproteobacterial GAOs, both enriched with propionate as the sole carbon source. Stable EBPR was achieved and the PAO population increased while the GAOs appeared to be out-competed. The results of this paper suggest that propionate may provide PAOs with a selective advantage over GAOs in the PAO-GAO competition, particularly through the minimisation of Competibacter Propionate may be a more suitable substrate than acetate for enhancing phosphorus removal in EBPR systems. (c) 2005 Elsevier B.V. All rights reserved.

The detection of environmental autoinducing peptide quorum-sensing genes from an uncultured Clostridium sp in landfill leachate reactor biomass

Aims: To elucidate whether a dominant uncultured clostridial (Clostridium thermocellum-like) species in an environmental sample (landfill leachate), possesses an autoinducing peptide (AIP) quorum-sensing (QS) gene, although it may not be functional. Methods and Results: A modified AIP accessory gene regulator (agr)C PCR protocol was performed on extracted DNA from a landfill leachate sample (also characterized by 16S rRNA gene cloning) and the PCR products were cloned, sequenced and phylogenetically analysed. It appeared that two agrC gene phylotypes existed, most closely related to the C. thermocellum agrC gene, differing by only 1 bp. Conclusions: It is possible to specifically identify and characterize the agrC AIP QS gene from uncultured Firmicutes (C. thermocellum-like) bacteria derived from environmental (landfill leachate) sample. Significance and Impact of the Study: This is the first successful attempt at identifying AIP QS genes from a cellulolytic environment (landfill). The agrC gene was identified as being most closely related to the C. thermocellum agrC gene, the same bacterium identified as being dominant, according to 16S rRNA gene cloning and subsequently fluorescence in situ hybridization analyses, in the same biomass.

Two-stage thermophilic-mesophilic anaerobic digestion of waste activated sludge-from a biological nutrient removal plant

A two-stage thermophilic-mesophilic anaerobic digestion pilot-plant was operated solely on waste activated sludge (WAS) from a biological nutrient removal (BNR) plant. The first-stage thermophilic reactor (HRT 2 days) was operated at 47, 54 and 60 degrees C. The second-stage mesophilic digester (HRT 15 days) was held at a constant temperature of 36-37 degrees C. For comparison with a single-stage mesophilic process, the mesophilic digester was also operated separately with an HRT of 17 days and temperature of 36-37 degrees C. The results showed a truly thermophilic stage (60 degrees C) was essential to achieve good WAS degradation. The lower thermophilic temperatures examined did not offer advantages over single-stage mesophilic treatment in terms of COD and VS removal. At a thermophilic temperature of 60 degrees C, the plant achieved 35% VS reduction, representing a 46% increase compared to the single-stage mesophilic digester. This is a significant level of degradation which could make such a process viable in situations where there is no primary sludge generated. The fate of the biologically stored phosphorus in this BNR sludge was also investigated. Over 80% of the incoming phosphorus remained bound up with the solids and was not released into solution during the WAS digestion. Therefore only a small fraction of phosphorus would be recycled to the main treatment plant with the dewatering stream.

The production of the enzyme dextransucrase by batch and continuous fermentation techniques

A review of the literature of work carried out on dextransucrase production, purification, immobilization and reactions has been carried out. A brief review has also been made of the literature concerning general enzyme biotechnology and fermentation technology. Fed-batch fermentation of the bacteria Leuconostoc mesenteroides NRRL B512 (F) to produce dextransucrase has formed the major part of this research. Aerobic and anaerobic fermentations have been studied using a 16 litre New Brunswick fermenter which has a 3-12 litre working volume. The initial volume of broth used in the studies was 6 litres. The results of the fed-batch fermentations showed for the first time that yields of dextransucrase are much higher under the anaerobic conditions than during the aerobic fermentations. Dextransucrase containing 300-350 DSU/cm3 of enzyme activity has been obtained during the aerobic fermentations, while in the anaerobic fermentations, enzyme yields containing 450-500 DSU/cm3 have been obtained routinely. The type of yeast extract used in the fermentation medium has been found to have significant effects on enzyme yield. Of the different types studied, the Gistex Standard was found to be the type that favoured the highest enzyme production. Studies have also been carried out on the effect of agitation rate and antifoam on the enzyme production during the anaerobic experiments. Agitation rates of up to 600 rpm were found not to affect the enzyme yield, however, the presence of antifoam in the medium led to a significant reduction in enzyme activity (less than 300 DSU/cm3). Scale-up of the anaerobic fermentations has been performed at up to the 1000 litre level with enzyme yields containing more than 400 DSU/cm3 of activity being produced. Some of the enzyme produced at this scale was used for the first time to produce dextran on an industrial scale via the enzyme route, with up to 99% conversion of sucrose to dextran being obtained. An attempt has been made at continuous dextransucrase production. Cell washout was observed to occur at dilution rates of greater than 0.4 h-1. Dextransucrase containing up to 25 DSU/cm3/h has been produced continuously.

Batch and continuous fermentation methods for the production of dextransucrase

The available literature concerning dextransucrase and dextran production and purification has been reviewed along with the reaction mechanisms of the enzyme. A discussion of basic fermentation theory is included, together with a brief description of bioreactor hydrodynamics and general biotechnology. The various fermenters used in this research work are described in detail, along with the various experimental techniques employed. The micro-organism Leuconostoc mesenteroides NRRL B512 (F) secretes dextransucrase in the presence of an inducer, sucrose, this being the only known inducer of the enzyme. Dextransucrase is a growth related product and a series of fed-batch fermentations have been carried out to extend the exponential growth phase of the organism. These experiments were carried out in a number of different sized vessels, ranging in size from 2.5 to 1,000 litres. Using a 16 litre vessel, dextransucrase activities in excess of 450 DSU/cm3 (21.67 U/cm3) have been obtained under non-aerated conditions. It has also been possible to achieve 442 DSU/cm3 (21.28 U/cm3) using the 1,000 litre vessel, although this has not been done consistently. A 1 litre and a 2.5 litre vessel were used for the continuous fermentations of dextransucrase. The 2.5 litre vessel was a very sophisticated MBR MiniBioreactor and was used for the majority of continuous fermentations carried out. An enzyme activity of approximately 108 DSU/cm3 (5.20 U/cm3) was achieved at a dilution rate of 0.50 h-1, which corresponds to the maximum growth rate of the cells under the process conditions. A number of continuous fermentations were operated for prolonged periods of time, with experimental run-times of up to 389 h being recorded without any incidence of contamination. The phenomenon of enzyme enhancement on hold-up of up to 100% was also noted during these fermentations, with dextransucrase of activity 89.7 DSU/cm3 (4.32 U/cm3) being boosted to 155.7 DSU/cm3 (7.50 U/cm3) following 24 hours of hold-up. These findings support the recommendation of a second reactor being placed in series with the existing vessel.

Heterogeneous catalysis in an oscillatory baffled flow reactor

The first demonstration of heterogeneous catalysis within an oscillatory baffled flow reactor (OBR) is reported, exemplified by the solid acid catalysed esterification of organic acids, an important prototypical reaction for fine chemicals and biofuel synthesis. Suspension of a PrSOH-SBA-15 catalyst powder is readily achieved within the OBR under an oscillatory flow, facilitating the continuous esterification of hexanoic acid. Excellent semi-quantitative agreement is obtained between OBR and conventional stirred batch reaction kinetics, demonstrating efficient mixing, and highlighting the potential of OBRs for continuous, heterogeneously catalysed liquid phase transformations. Kinetic analysis highlights acid chain length (i.e. steric factors) as a key predictor of activity. Continuous esterification offers improved ester yields compared with batch operation, due to the removal of water by-product from the catalyst, evidencing the versatility of the OBR for heterogeneous flow chemistry and potential role as a new clean catalytic technology. © The Royal Society of Chemistry 2013.

Novel input-output prediction approach for biomass pyrolysis

Biomass pyrolysis to bio-oil is one of the promising sustainable fuels. In this work, relation between biomass feedstock element characteristic and pyrolysis process outputs was explored. The element characteristics considered in this study include moisture, ash, fix carbon, volatile matter, carbon, hydrogen, nitrogen, oxygen, and sulphur. A semi-batch fixed bed reactor was used for biomass pyrolysis with heating rate of 30 °C/min from room temperature to 600 °C and the reactor was held at 600 °C for 1 h before cooling down. Constant nitrogen flow rate of 5 L/min was provided for anaerobic condition. Rice husk, Sago biomass and Napier grass were used in the study to form different element characteristic of feedstock by altering mixing ratio. Comparison between each element characteristic to total produced bio-oil yield, aqueous phase bio-oil yield, organic phase bio-oil yield, higher heating value of organic phase bio-oil, and organic bio-oil compounds was conducted. The results demonstrate that process performance is associated with feedstock properties, which can be used as a platform to access the process feedstock element acceptance range to estimate the process outputs. Ultimately, this work evaluated the element acceptance range for proposed biomass pyrolysis technology to integrate alternative biomass species feedstock based on element characteristic to enhance the flexibility of feedstock selection.