Development and characterization of 16 microsatellites for the Neotropical catfish Pseudoplatystoma reticulatum and cross species analysis

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Stuart Hall, cultural studies e a nostalgia da dominação hegemônica

Stuart Hall é um dos poucos autores contemporâneos que ainda se apóia no conceito de ideologia. Ela interfere na língua falada definindo limites, regulando a forma discursiva com a intenção de estabilizá-la e congelá-la. Ela domina o bloco histórico e a estratégia seria a de unir as camadas populares, a partir de dentro, para que estas possam fortificar-se e combatê-la. Mas todo esse esquema tem sabor nostálgico, como se houvesse uma manobra dos poderosos e os dominados não se identificassem com os signos de poder e prestígio. Como no caso da “leitura crítica”, não seria o caso de modernizar Stuart Hall?

Expression profiling and cross-species RNA interference (RNAi) of desiccation-induced transcripts in the anhydrobiotic nematode Aphelenchus avenae

Abstract Background Some organisms can survive extreme desiccation by entering a state of suspended animation known as anhydrobiosis. The free-living mycophagous nematode Aphelenchus avenae can be induced to enter anhydrobiosis by pre-exposure to moderate reductions in relative humidity (RH) prior to extreme desiccation. This preconditioning phase is thought to allow modification of the transcriptome by activation of genes required for desiccation tolerance. Results To identify such genes, a panel of expressed sequence tags (ESTs) enriched for sequences upregulated in A. avenae during preconditioning was created. A subset of 30 genes with significant matches in databases, together with a number of apparently novel sequences, were chosen for further study. Several of the recognisable genes are associated with water stress, encoding, for example, two new hydrophilic proteins related to the late embryogenesis abundant (LEA) protein family. Expression studies confirmed EST panel members to be upregulated by evaporative water loss, and the majority of genes was also induced by osmotic stress and cold, but rather fewer by heat. We attempted to use RNA interference (RNAi) to demonstrate the importance of this gene set for anhydrobiosis, but found A. avenae to be recalcitrant with the techniques used. Instead, therefore, we developed a cross-species RNAi procedure using A. avenae sequences in another anhydrobiotic nematode, Panagrolaimus superbus, which is amenable to gene silencing. Of 20 A. avenae ESTs screened, a significant reduction in survival of desiccation in treated P. superbus populations was observed with two sequences, one of which was novel, while the other encoded a glutathione peroxidase. To confirm a role for glutathione peroxidases in anhydrobiosis, RNAi with cognate sequences from P. superbus was performed and was also shown to reduce desiccation tolerance in this species. Conclusions This study has identified and characterised the expression profiles of members of the anhydrobiotic gene set in A. avenae. It also demonstrates the potential of RNAi for the analysis of anhydrobiosis and provides the first genetic data to underline the importance of effective antioxidant systems in metazoan desiccation tolerance.

The Turkish Drawbridge: European Integration and the Cultural Economics of National Planning

This article examines the relations between the Turkish State Planning Organisation (SPO) and the Western economic system during the first two decades of national planning in Turkey (1960–1980). It traces how the SPO, established with the guidance and full endorsement of international economic institutions came to vehemently oppose Turkish participation in one of their pillars: the European Economic Community (EEC), the predecessor of the European Union. It argues that the shift in the SPO's world-view was founded upon two distinct understandings of the Turkish nation and its development, situates these understandings within the intellectual history of Turkey's past ambivalence towards the West, and, in doing so, provides a historical case-study of the ideological clash between modernisation and dependency theories of development.

Strengthening the Reporting of Observational Studies in Epidemiology (STROBE): explanation and elaboration

Much medical research is observational. The reporting of observational studies is often of insufficient quality. Poor reporting hampers the assessment of the strengths and weaknesses of a study and the generalizability of its results. Taking into account empirical evidence and theoretical considerations, a group of methodologists, researchers, and editors developed the Strengthening the Reporting of Observational Studies in Epidemiology (STROBE) recommendations to improve the quality of reporting of observational studies.The STROBE Statement consists of a checklist of 22 items, which relate to the title, abstract, introduction, methods, results and discussion sections of articles. Eighteen items are common to cohort studies, case-control studies and cross-sectional studies and four are specific to each of the three study designs. The STROBE Statement provides guidance to authors about how to improve the reporting of observational studies and facilitates critical appraisal and interpretation of studies by reviewers, journal editors and readers.This explanatory and elaboration document is intended to enhance the use, understanding, and dissemination of the STROBE Statement. The meaning and rationale for each checklist item are presented. For each item, one or several published examples and, where possible, references to relevant empirical studies and methodological literature are provided. Examples of useful flow diagrams are also included. The STROBE Statement, this document, and the associated web site (http://www.strobe-statement.org) should be helpful resources to improve reporting of observational research.

Strengthening the Reporting of Observational Studies in Epidemiology (STROBE): explanation and elaboration

Much medical research is observational. The reporting of observational studies is often of insufficient quality. Poor reporting hampers the assessment of the strengths and weaknesses of a study and the generalisability of its results. Taking into account empirical evidence and theoretical considerations, a group of methodologists, researchers, and editors developed the Strengthening the Reporting of Observational Studies in Epidemiology (STROBE) recommendations to improve the quality of reporting of observational studies. The STROBE Statement consists of a checklist of 22 items, which relate to the title, abstract, introduction, methods, results and discussion sections of articles. Eighteen items are common to cohort studies, case-control studies and cross-sectional studies and four are specific to each of the three study designs. The STROBE Statement provides guidance to authors about how to improve the reporting of observational studies and facilitates critical appraisal and interpretation of studies by reviewers, journal editors and readers. This explanatory and elaboration document is intended to enhance the use, understanding, and dissemination of the STROBE Statement. The meaning and rationale for each checklist item are presented. For each item, one or several published examples and, where possible, references to relevant empirical studies and methodological literature are provided. Examples of useful flow diagrams are also included. The STROBE Statement, this document, and the associated Web site (http://www.strobe-statement.org/) should be helpful resources to improve reporting of observational research.

Family satisfaction in the intensive care unit: cross-cultural adaptation of a questionnaire

PURPOSE: Family needs and expectations are often unmet in the intensive care unit (ICU), leading to dissatisfaction. This study assesses cross-cultural adaptability of an instrument evaluating family satisfaction in the ICU. MATERIALS AND METHODS: A Canadian instrument on family satisfaction was adapted for German language and central European culture and then validated for feasibility, validity, internal consistency, reliability, and sensitivity. RESULTS: Content validity of a preliminary translated version was assessed by staff, patients, and next of kin. After adaptation, content and comprehensibility were considered good. The adapted translation was then distributed to 160 family members. The return rate was 71.8%, and 94.4% of questions in returned forms were clearly answered. In comparison with a Visual Analogue Scale, construct validity was good for overall satisfaction with care (Spearman rho = 0.60) and overall satisfaction with decision making (rho = 0.65). Cronbach alpha was .95 for satisfaction with care and .87 for decision-making. Only minor differences on repeated measurements were found for interrater and intrarater reliability. There was no floor or ceiling effect. CONCLUSIONS: A cross-cultural adaptation of a questionnaire on family satisfaction in the ICU can be feasible, valid, internally consistent, reliable, and sensitive.

[Strengthening the reporting of observational studies in epidemiology (STROBE): explanation and elaboration]

Much medical research is observational. The reporting of observational studies is often of insufficient quality. Poor reporting hampers the assessment of the strengths and weaknesses of a study and the generalisability of its results. Taking into account empirical evidence and theoretical considerations, a group of methodologists, researchers, and editors developed the Strengthening the Reporting of Observational Studies in Epidemiology (STROBE) recommendations to improve the quality of reporting of observational studies. The STROBE Statement consists of a checklist of 22 items, which relate to the title, abstract, introduction, methods, results and discussion sections of articles. Eighteen items are common to cohort studies, case-control studies and cross-sectional studies and four are specific to each of the three study designs. The STROBE Statement provides guidance to authors about how to improve the reporting of observational studies and facilitates critical appraisal and interpretation of studies by reviewers, journal editors and readers. This explanatory and elaboration document is intended to enhance the use, understanding, and dissemination of the STROBE Statement. The meaning and rationale for each checklist item are presented. For each item, one or several published examples and, where possible, references to relevant empirical studies and methodological literature are provided. Examples of useful flow diagrams are also included. The STROBE Statement, this document, and the associated Web site (http://www.strobe-statement.org/) should be helpful resources to improve reporting of observational research.

Transnational Migration and Cross-border Mobility in the Serbian-Hungarian Border Region

The study is aiming to present migrant groups in the Serbian-Hungarian border region which are playing a role in forming transnational migration, ransnational networks and transnational spaces. Transnational migrants are using material, cultural, social and symbolical capital in various forms. The everyday activities of the people as well as their mobility, emotional attachments, and economic relationships have contributed to a transnational region the cross-border contacts in the last century were always present, even in spite of political and historical changes, only their intensity, political charge and tension has been changing. The asymmetries defining the border region has been reflected in different directions, places, time and in different volume. In this specially built and constantly reorganising scope, people who were living their everyday life have tried to use the differences for their benefit, and to improve their possibilities. With their transnational lifestyles and cross-border networks, they substantially contribute to the stability and prosperity of the cross-border region.

Adolescents' family models: A cross-cultural study

Die vorliegende kulturvergleichende Arbeit befasst sich mit den Familienmodellen Jugendlicher in zehn Kulturen auf Basis eines typologischen Ansatzes und eines Mehrebenenansatzes. Sie leistet damit einen empirischen Beitrag zur Theorie der Familienmodelle im kulturellen Wandel nach Kagitcibasi (2007). Diese Theorie postuliert die Existenz dreier idealtypischer Familienmodelle: ein Familienmodell der Independenz, das in industrialisierten westlichen Gesellschaften vorherrscht, ein Familienmodell der (vollständigen) Interdependenz, das in nicht-industrialisierten agrarischen Kulturen zu finden ist, und als Synthese aus den beiden genannten ein Familienmodell der emotionalen Interdependenz. Letzteres entwickelt sich der Theorie zufolge, wenn nicht-industrialisierte kollektivistische Kulturen Modernisierungsprozessen ausgesetzt sind. Traditionelle Kulturen mit einem Familienmodell der (vollständigen) Interdependenz sollen sich also im Zuge gesellschaftlicher Modernisierungsprozesse nicht notwendigerweise hin zum Familienmodell der Independenz entwickeln, sondern zu einem emotional-interdependenten Modell, das erlaubt, Autonomie mit enger interpersoneller Verbundenheit zu vereinen. Diese Annahme steht im Widerspruch zu klassischen modernisierungstheoretischen Annahmen und wurde in bisherigen Studien nur unzureichend geprüft. In einem typologischen Mehrebenen-Ansatz sollen Profile allgemeiner und familienbezogener Werthaltungen Jugendlicher identifiziert werden, die mit den drei idealtypischen Familienmodellen theoretisch verbunden werden können. In einem zweiten Schritt sollen diese Wertemuster durch verhaltensnähere Familienmodellindikatoren validiert werden. Die Daten für diese Arbeit stammen aus der von der Deutschen Forschungsgemeinschaft geförderten kulturvergleichenden und interdisziplinären Value of Children and Intergenerational Relations Studie (Trommsdorff, 2001) und umfassen eine Stichprobe von 2566 Jugendlichen aus der Volksrepublik China, Deutschland, Frankreich, Indien, Indonesien, Israel, Japan, Südafrika, der Schweiz und der Türkei. Zur Identifikation der angenommen Familienmodell-Werteprofile wurden Cluster-Analysen auf der Kultur- und auf der Individualebene durchgeführt. Auf beiden Analyseebenen zeigten sich drei Werteprofile, die dem erwarteten Muster der drei idealtypischen Familienmodelle entsprachen. Das Familienmodell der emotionalen Interdependenz zeigte dabei bezüglich der Werthaltungen, die emotionale Interdependenzen in der Familie widerspiegeln ähnlich hohe Werte wie das Familienmodell der (vollständigen) Interdependenz, dagegen zeigte es ähnliche Werte wie das Familienmodell der Independenz in Bezug auf Autonomie und materielle Interdependenz widerspiegelnde Werte. In Kulturen, die auf der Kulturebene ein bestimmtes Familienmodell-Werteprofil aufwiesen befanden sich zudem überwiegend Jugendliche, die das jeweils korrespondierende Werteprofil auf der individuellen Analyseebene aufzeigten. Zur Validierung der erhaltenen Familienmodell-Werteprofile wurden diese zu verhaltensnäheren Merkmalen von Familienmodellen in Beziehung gesetzt. Die Vorhersage erfolgte auch hier jeweils getrennt für die Kultur- und Individualebene. Mit Hilfe multinomialer logistischer Modelle wurde zunächst der Effekt der Familienmodell-Werteprofile auf die Bereitschaft der Jugendlichen überprüft, ihren Eltern bei der Hausarbeit zu helfen. Die Ergebnisse zeigten einen starken Effekt der Familienmodell-Werteprofile auf beiden Analyseebenen: fast alle Jugendlichen mit einem Familienmodell der (vollständigen) Interdependenz würden ihren Eltern helfen statt sich wie geplant mit ihren Freunden zu treffen, wohingegen nur zwei Drittel bzw. nur ein Drittel der Jugendlichen mit einem Familienmodell der emotionalen Interdependenz bzw. der Independenz der Bitte der Eltern folgen würde. In Bezug auf die familienbezogenen Zukunftsorientierungen Jugendlicher zeigte sich für die Pläne für eine zukünftige Heirat/Beziehung, dass Jugendliche mit einem Familienmodell der (vollständigen) Interdependenz den stärksten Heiratswunsch hatten. Jugendliche mit einem Familienmodell der emotionalen Interdependenz waren bezüglich dieser Frage am unsichersten, und Jugendliche mit einem Familienmodell der Independenz äußerten die stärkste Ablehnung in dieser Frage. In Bezug auf Pläne, später eigene Kinder zu haben zeigten sich ähnliche Effekte, die aber insgesamt schwächer ausfielen. Insgesamt zeigte sich trotz der Unterschiede eine hohe familienbezogene Zukunftsorientierung in allen Familienmodell-Werteprofilen sowie in allen Kulturen. Bezüglich der Sohn- bzw. Tochterpräferenz zeigten sich keine Effekte der Familienmodell- und Kulturzugehörigkeit. Die Ergebnisse der Studie stärken die umstrittene Validität insbesondere des Familienmodells der emotionalen Interdependenz. Ob es sich hierbei im Sinne der Theorie von Kagitcibasi um ein synthetisches Konvergenzmodell oder eher um ein Übergangsmodell handelt, muss zukünftige Forschung zeigen.

Governance of Digital Game Environments and Cultural Diversity : Transdisciplinary Enquiries

Digital game environments are of increasing economic, social and cultural value. As their influence on diverse facets of life grows, states have felt compelled to intervene and secure some public interests. Yet, the contours of a comprehensive governance model are far from recognisable and governments are grappling with the complexity and fluidity of online games and virtual worlds as private spaces and as experimentation fields for creativity and innovation. This book contributes to a more comprehensive and fine-grained understanding of digital game environments, which is a precondition for addressing any of the pressing governance questions posed. Particular attention is given to the concept and policy objective of cultural diversity, which also offers a unique entry point into the discussion of the appropriate legal regulation of digital games. Governance of Digital Game Environments and Cultural Diversity will be of interest to researchers of media law, internet law and governance, cultural studies, anthropology and sociology. As the book addresses a highly topical theme, it will attract the attention of policymakers at national, regional and international levels and will also serve as a great resource tool for scholars in new media and, in particular, digital games and virtual worlds.

Cross-cultural analysis of type D (distressed) personality in 6222 patients with ischemic heart disease: a study from the International HeartQoL Project

BACKGROUND Type D (distressed) personality, the conjoint effect of negative affectivity (NA) and social inhibition (SI), predicts adverse cardiovascular outcomes, and is assessed with the 14-item Type D Scale (DS14). However, potential cross-cultural differences in Type D have not been examined yet in a direct comparison of countries. AIM To examine the cross-cultural validity of the Type D construct and its relation with cardiovascular risk factors, cardiac symptom severity, and depression/anxiety. METHODS In 22 countries, 6222 patients with ischemic heart disease (angina, 33%; myocardial infarction, 37%; or heart failure, 30%) completed the DS14 as part of the International HeartQoL Project. RESULTS Type D personality was assessed reliably across countries (αNA>.80; αSI>.74; except Russia, which was excluded from further analysis). Cross-cultural measurement equivalence was established for Type D personality at all measurement levels, as the factor-item configuration, factor loadings, and error structure were not different across countries (fit: CFI=.91; NFI=.88; RMSEA=.018), as well as across gender and diagnostic subgroups. Type D personality was more prevalent in Southern (37%) and Eastern (35%) European countries compared to Northern (24%) and Western European and English-speaking (both 27%) countries (p<.001). Type D was not confounded by cardiac symptom severity, but was associated with a higher prevalence of hypertension, smoking, sedentary lifestyle, and depression. CONCLUSION Cross-cultural measurement equivalence was demonstrated for the Type D scale in 21 countries. There is a pan-cultural relationship between Type D personality and some cardiovascular risk factors, supporting the role of Type D personality across countries and cardiac conditions.

Strengthening the Reporting of Observational Studies in Epidemiology (STROBE): Explanation and elaboration

Much medical research is observational. The reporting of observational studies is often of insufficient quality. Poor reporting hampers the assessment of the strengths and weaknesses of a study and the generalisability of its results. Taking into account empirical evidence and theoretical considerations, a group of methodologists, researchers, and editors developed the Strengthening the Reporting of Observational Studies in Epidemiology (STROBE) recommendations to improve the quality of reporting of observational studies. The STROBE Statement consists of a checklist of 22 items, which relate to the title, abstract, introduction, methods, results and discussion sections of articles. Eighteen items are common to cohort studies, case-control studies and cross-sectional studies and four are specific to each of the three study designs. The STROBE Statement provides guidance to authors about how to improve the reporting of observational studies and facilitates critical appraisal and interpretation of studies by reviewers, journal editors and readers. This explanatory and elaboration document is intended to enhance the use, understanding, and dissemination of the STROBE Statement. The meaning and rationale for each checklist item are presented. For each item, one or several published examples and, where possible, references to relevant empirical studies and methodological literature are provided. Examples of useful flow diagrams are also included. The STROBE Statement, this document, and the associated Web site (http://www.strobe-statement.org/) should be helpful resources to improve reporting of observational research.