Use of dopamine antagonists on induced spawning of Indian major carps

To test the efficacy of the technique of using dopamine-antagonists with pituitary extracts, experiments were conducted in July/August '93, at the CIFE Fresh water Fish Farm, Powerkheda. In all, 35 sets (1 female x 2 male in each set) were tried individually. 19 sets were treated with Domperidone (DOM) and Carp Pituitary Extract (CPE) and they constituted the experimental sets, while the rest 16 were treated with CPE and formed the control sets. The breeding, complete ovulation and hatching was 84.2%, 87.5% and 85.7%, respectively in the experimental sets whereas it was 93.7%, 60% and 72.7%, respectively in case of control sets. Experimental sets yielded 1.20 lakh spawn/kg body wt. (female) as compared to 0.83 lakh spawn kg body wt. (female) received from control sets on average basis. When DOM was used at 50-60% there was 100% breeding success but when increased to 70%, breeding rate fell to 66%. In mass breeding of Catla in the circular hatchery DOM mixed with CPE in 50:50 ratio gave excellent results.

National report of the frame survey 2006 in the Ugandan part of Lake Victoria

Fisheries Frame surveys have been carried out on Lake Victoria biannually since 2000 to determine· the number of fishers, fish landing sites, facilities at the landing sites. Information on the composition fishing crafts, their mode of propulsion, fishing gears and the fish species they target is also collected. This information is used to guide development and management of the lake's fisheries. The results of the four surveys carried out since 2000 show that the number of landing sites has decreased by 24% from 597 in 2000 to 481 in 2006, mainly as result of reorganisation of landing sites into Beach Management Units (BMUs). The fish landing sites continue to have inadequate facilities to service the fisheries industry e.g. in the 2006 survey only 11.2% of landing sites had fish shades; <0.6% had cold rooms; 3.5% had electricity; and only 35.5% had toilet facilities. Similarly, only 11.4% of landing sites had portable water; 2.5% had jetties, 3.7% had 'fish stores; and 36% were accessible by all weather roads. There is need to improve facilities servicing fisheries at landing sites, with major emphasis on sanitary facilities, especially toilets and portable water. The BMUs should be sensitised to prioritise the construction and utilisation of public toilet facilities at their respective landing sites. The ultimate aim should be to have public toilets at all fish landing sites. The trend of the various indicators of fishing effort has continued upwards: The total number of fishers increased by 43.5% from 37,721 in 2004 to 54,148 in 2006 and the number of fishing crafts increased (43.9%) from 16,775 in 2004 to 24,148 in 2006 over the same period. The total number of gillnets increased by 28.6% from 458,597 in 2004 to 589,777 in 2006 and the number of long line hooks increased by 136% from 968,848 to 2,285,609. The number of fishing crafts using outboard engines also increased from 3,173 in 2004 to 5,047 in 2006 suggesting that more fishers were going far in search of fish. There is still a large number of illegal gears especially beach seines, which increased by 58.8% from 954 in 2004 to 1420 in 2006. Efforts to remove these very destructive gears should be stepped up. There was also larger increase in number of illegal gillnets <5 inch mesh size. These increased by 63% from 56,246 in 2004 to 91,740 in 2006 compared with 23.8% increase of gillnets ~5 inch mesh size. There were also large increases in the numbers of gillnets of 5 and 5% inch mesh size, which increased by 48% and 130% from 2004 to 2006 implying a trend towards use of smaller gillnet mesh sizes. The number of traps which are used in shallow vegetated areas, flood plains and river mouths to target tilapiines and riverine species decreased drastically from 5,361 traps in 2004 to only 499 traps in 2006, a decrease of 974%, a phenomenon attributed to the receding water levels which have left the shallow vegetated areas dry. A total of 17,475 fishing crafts, 72% of all fishing crafts, in the Ugandan part of the lake are still using paddles and the. number of parachute crafts is also still very high, (Le. 5,064) comprising a high proportion (21 %) of the total number of fishing. There is need to promote the use of large fishing crafts with sails or a combination of sail and outboard motor. The Mukene fishery in the Ugandan waters of Lake Victoria has remained underdeveloped with only 9% of all fishing crafts operating in this fishery. Also less than 2% of fishing crafts with sails or motor operate in this fishery which implies that it is limited to near shore waters. Effort should be made to develop this fishery as it appears to have high potential, especially in deep offshore waters which are hardly fished.

Growth and yield of GIFT (Oreochromis niloticus) and Thai silver barb (Barbodes gonionotus Bleeker) in rice fields and their effects on the yield of rice

(GIFT) (Oreochromis niloticus) and Silver barb (Barbodes gonionotus) in rice fields and their effects on the yield of rice was carried out in nine experimental rice plots. Three treatments viz., treatment-1 with 0. niloticus (T1), treatment-2 with B. gonionotus (T2) and treatment- 3 was kept as control (T3, without fish) were used in this study. Fertilizers such as, Urea (178 kg ha-1), T.S.P (125 kg ha-1) and M.P. (67 kg ha-1) were applied in each treatment. The fishes were stocked @ 6250 ha·1 and the experiment was continued for a period of 107 days. The values of water quality parameters such as, water temperature, dissolved oxygen, pH and chlorophyll-a were found within suitable level. Between the two species, higher specific growth rate was recorded in 0. niloticus than that of B. gonionotus. But B. gonionotus showed much higher survival (72%) than that of 0. niloticus (35%). Similar to survival, higher production (244 kg ha-1) and income (Tk. 6399 ha-1) were recorded in B. gonionotus than those of 0. niloticus (142.8 kg ha'1 and Tk. 2137 ha-1). Significant differences (p

Minimum dissolved oxygen tolerance of four different sizes of milkfish

Four size groups of milkfish were tested, 4-18 g, 20-34 g, 35-95 g and 200-300 g. A number of fish from each group were placed separately in identical 1.2 m2 wooden tanks containing seawater filled up to 30 cm depth. The aggregate weight of fish per size group was approximately 1 kg. The fish were held for 72 h, fed with lab-lab and provided with continuous aeration to allow recovery from stress during transport and handling. After the recovery period, aeration was stopped and 200 g of the fine rice bran was spread over the water in each tank creating a film of bran particles on the water surface. This was designed to speed up depletion of dissolved oxygen considering the combined effects of the screening-off of sunlight, the reduction of air-water interface and the breakdown of the bran particles. It is probable that stress on milkfish in brackishwater ponds could start when oxygen level drops to about 1.4 ppm. A further decrease to 0.04 ppm could produce a total kill of all specimens above 4 grams with marketable size and bigger size fish dying first.

Assessment of the heavy metals among suspended particulates and dissolved phases in Suez Canal water

The concentrations and distribution of particulate and dissolved heavy metals, viz: Cu, Zn, Pb, Cd, Fe and Mn have been determined seasonally during 2003 in water samples collected from the Suez Canal. The presented data clarifies that the metals exhibited clear differences in their distribution between particulate and dissolved forms. The concentration of particulate heavy metals ranged between 0.09-3.13, 0.57-15.02, 0.18-3.87, 0.02-0.73, 2.74-49.62 and 0.15-5.08 µg/L for Cu, Zn, Pb, Cd, Fe and Mn, respectively. In the same respect, these values for dissolved forms were 0.28-4.12, 0.57-9.08, 0.27-2.50, 0.02-1.24, 1.94-42.50 and 0.11-3.65 µg/L. The concentrations of particulate metals viz: Zn, Pb, Cd, Fe and Mn were high was compared to the dissolved forms. Dissolved copper, rather than particulate, showed the highest percentage of total copper. The particulate forms of Pb, Cd, Fe and Mn always had higher concentrations than the dissolved forms during the course of study except in the summer season. The northern part of the Suez Canal at Port Said showed mean concentrations of particulate and dissolved Cu=1.43 and 2.10, Zn=8.61 and 3.17, Pb=1.72 and 1.23, Cd=0.35 and 0.35, Fe= 23.49 and 15.83 and Mn=2.09 and 1.82 µg/L. These high concentrations may be attributed to the greater activities, particularly loading and unloading operations at Port Said harbour s industrial effluents and domestic drainage of Port Said city. In contrast, the Sinai side could be considered as reference site, as it was almost clean, i.e., without harmful outfalls, where Cu=0.16 and 0.56, Zn=2.14 and 0.94, Pb=6.29 and 3.44, Cd=0.055 and 0.088, Fe=6.29 and 3.44 and Mn=0.56 and 0.26 µg/L for particulate and dissolved metals respectively.

Evaluation of heavy metals along the Mediterranean coastal waters of the Nile Delta region, Egypt

This work focuses on four marine sites in the Mediterranean Sea around the Nile Delta, Egypt. Surface water samples were collected seasonally during 2003. The concentrations of some heavy metals in dissolved form (Fe, Mn, Zn, Cu, Ni and Pb) are evaluated. The levels of heavy metals in the coastal waters were 11.92-30.4512µglˉ¹ for Fe; 5.79-17.36 µglˉ¹ for Zn; 0.30-0.83µglˉ¹ for Cu; 0.51-2.90µglˉ¹ for Ni and 0.53-10.31µglˉ¹ for Pb. These are compared, with sites in the estuaries and outlets of the Nile Delta. Fe (19.72-60.33µglˉ¹); Mn (12.63-35.60µglˉ¹); Zn (2.67-22.00); Cu (0.56-1.67µglˉ¹); Ni (1.43-3.73µglˉ¹); Pb (1.72-59.7µglˉ¹). The results showed a remarkable decrease in the concentrations of different heavy metals with increased salinity. Comparing the present data with the minimal risk concentration reported by WQC, the distribution of heavy metals was significantly lower in coastal sea water of the Mediterranean Sea off Egypt. The study indicated also that the average contents of Ni and Pb are slightly high in the area of water exchange than those reported by WQC.

Indian squid, Uroteuthis duvauceli, is an important commercial marine fauna in the coastal waters of Oman Sea

About 3600 specimens were collected by bottom trawl at 15 sampling stations. 24 biometric characters were measured for each specimens at the laboratory.. Microscopic cross – sections of statolith were used for age determination. Sex determination and fecundity were determined. Population dynamics parameters as well as stock as stock assessment including cohort analysis were estimated using FISAT software. The findings showed that Dorsal Mantle Length (DML) and Body weight (BW) of the Indian squid were 133.9 ± 0.78 mm and 99.61 ± 0.95 g respectively. Strong correlation was found between these 2 variables (R2 = 0.90). The maximum age was 5 years. Relationship between DML and age was highly significantly of p ≤ 0.05. Overall sex ratio (M: F = 0.52) was significantly different from the expected 1:1 ratio (p ≤ 0.05). The ovary weight and nidamental glands weight were 7.72 ± 0.0006 g and 3.07 ± 0.0003g respectively. Absolute and relative fecundity of the Indian squid were found to be 122733 ± 30.87 and 2348 ± 0.4 respectively. GSI were 14.35 in April and 8.63 in July. This squid is therefore a spring spawner. The infinite dorsal mantle length were 258.62 mm for females, 194.72 mm for males and 252.02 for both sexes respectively. For population growth and mortality parameters; K (0.65 per year for both sexes, 0.85 per year for males, 0.65 per year for females); t0 (0.24year for both sexes, 0.22 year in females, 0.26 year in male); φ` (2.30 in both sexes, 2.47 for males, 2.37 for females); Z (1.17 per year for both sexes, 1.10 per year in females, 1.39 per year, in males); M (0.70 per year for both sexes, 0.90 for males, 0.67 for females); F(0.27 per year for both sexes, 0.27 per year in males, 0.195 per year in females). Exploitation coefficient were 0.51 per year for both sexes, 0.57 per year males and 0.51 per year females respectively. The results indicates that since the Indian squid is a short live aquatic organism, therefore, the exploitation coefficient could be raised to 0.7 per year. The analysis showed that total biomass and MSY were 10103.5 ton and 2576.4 ton respectively. These findings are the first study of its sort about the Indian squid in the coastal waters of Oman Sea as well as North-West of Indian Ocean.

Temporal and spatial distribution of phytoplankton with emphasis on Skeletonema costatum in the Mathamuhuri river – estuary (Chakaria mangrove ecosystem), Bangladesh

A total of 91 species under 44 genera were identified among the phytoplankton community during the course of one year's investigation between May 1982 and April 1983. Bacillariophyta was the most dominant group with 72 specie, Chlorophyta 11 spp, Cyanophyta 6 spp and Pyrrophyta was represented by 2 species. The yearly percentage composition of 4 groups of phytoplankton in order of abundance were Bacillariophyta 50.77%, Cyanophyta 47.70%, Chlorophyta 1.5% and Pyrrophyta 0.02%. The highest densities of phytoplankton were recorded in monsoon months (June-July) with a peak in July (31550 cells/l) and the minimum in February (770 cells/1). Higher concentration of phytoplankton was recorded at station 2, nearer to the Chakaria Sundarbans (mangroves), but abundance of phytoplankton showed no significant difference in the two stations (Mann Whitney U test, P=0.64, Z=-0.642, U=64). Phytoplankton population in this area were positively correlated with rainfall (r=0.655, P=<0.5, df.22) and water temperature (r=0.523, P=<0.05). Skeletonema costatum was the dominant member of phytoplankton and occupied 35.23% of the annual population and occurred throughout the period of study except in September and January. Its abundance was recorded during the monsoon months (April- July) with a maximum density (24185 cells/l) in July. No significant correlation was found between abundance of S. costatum and the hydro-meteorological parameters recorded in the Chakaria mangrove area.

The study of different protein in diet and water salinity on growth and survival rate in Pacific white shrimp (Litopenaeus vannamei)

In this research reared white western shrimp (Litopenaeus vannamei ,Boone, 1931) with five diet with five different protein level contain 20%, 25%, 30%, 35% and 40% and three salinity level contain 15-17 ppt, 27-30 ppt,and 40-45 ppt researched protein percent effect and water salinity on growth, survival, feed conversion ratio, hemolymph osmolatity, hemolymph protein and corpse protein contain. In this research was 15 sorrow with 3 repeat and used from 45 tanks with 300 liters capacity. Shrimps first weight average was about 2 grams and after 60 days culture cropped down results: Shrimps biomass growth in 15-17 ppt salinity was higher than anther salinities who had meaning different with growth in 40-45 ppt salinity ( p< 0.05). But hadn’t meaning different with growth 27-30 ppt salinity. survival rate in 15-17 ppt salinity was 97.03 who was lower than another salinities. survival percent in 24-30 ppt salinity and 40-45 ppt salinity was 99.33% Highest biomass growth in different diets was in diet number 5 with 40 percent protein that it had meaning different with another diets (p<0.05) . although with informed to product expense in different diets. One kilogram shrimp product expense in different diets hadn’t meaning different (P<0.05) Survival rate in different diets hadn’t meaning different lowest feed conversion ratio was 1.67 in 15-14 salinity that hadn’t meaning different with another salinities also corpse protein quantity in different salinities and different diets hadn’t meaning different. Hemolymph Osmolality in 15-17 ppt salinity was 573.88 mOsm/kg had meaning different with hemolymph osmolality in 27-30 ppt salinity that was 650. 380 mOsm/kg and in 40-45 ppt salinity was 630.38 mOsm/kg. Hemolymph protein in 15-17 ppt salinity was 124.72 mg/ml had meaning different with hemlymph protein in 27-30 ppt salinity that was 136.52 mg/ml but hadn’t meaning different with hemolymph protein in 40-45 ppt salinity that was 128.84 mg/ml. Hemolymph protein in different diets hadn’t meaning different (p<0.05). Keywords: shrimp, Litopenaeus vannamei, protein , salinity, growth, survival rate, FCR, hemolymph osmolality, hemolymph protein.

Contribución al estudio biológico de Loligo brasiliensis Bl

A total of 592 individuals of Loligo brasiliensis from the Mar del Plata coastal fishing area (Buenos Aires prov., Argentina) have been studied during the 1961-1964 period. From a morphological point of view the population appears to be uniform and homogeneus. A brief description of this species is given in this paper since references in the literature are scarce from the time at which Blainville (1923) first described it. The only further references are found in D'Orbigny (1835), and Ferrusac (1839), and in Hoyle (1886), and Tyron Pilsbry (1879). In this paper the species was mentioned only as a bibliographical reference on morphological or biological conditions has been found in the literature. The distribution of this species ranges from Cuba, Brazil, Uruguay to the Argentine coast, probably down to the Gulf of San Jorge. The samples had been studied with respect to various body measurements by classifing the individuals in total length classes, since body length was considered the most significant measurement. The condition factor K has been calculated for different sexes and ages, for the various length classes. The results lead to the conclusion that the smaller the length the higher is the value obtained for K and viceversa. This is due to the fact that the length of the tentacle increases considerably with increasing size. Since the tentacle are quite light the factor K diminishes accordingly. The condition factor increases considerably from December to April with an average of 0.42, decrease and becomes stable from March to October, with an average of 0.30. This is a consequence of the ripening of the sex glands. The sex-ratios are as following: year 1961, 42 % female, 42 % male; year 1962, 51 % female, 45 % male; year 1963, 46 % female, 53 % male; year 1964, 26 % female, 42 % male, 32 % indif. The great percentage of 72 undifferentiated young individuals in the 1964 (March) sampling increases the ratio of undifferentiation. A short morphological description of both ovules and spermatozoos is given. An examination of the sex glands leads to the following conclusions: a) male and female sex gland in a preparatory stage during the whole year; b) the highest percentage of ripe glands is found through, November-March; e) the spawning appears to precede rather slowly, but this certain since the spawning environment does not coincide with the natural habitat of the species. Few spawning individuals were found; d) sexual differentiation begins at body lenght from 30 to 40 mm; i.e. a total length of approximately 145 mm. At a body length of 70 mm. the hectocotilication (sexual character) begins to appear. In June 1962, a sample gathered at Rawson (Chubut) was analyzed. The conclusion was reached that the sex glands in this population are in an earlier stage of development in comparison with those from the Mar del Plata area. Also the average for the factor K which were found to be 0.17 for females and 0.19 for male, are rather low for that date. These physiological facts are possibly related to morphological differences which will be pointed out in a forthcoming publication. Some very typical associations with Artemesia longinaris and Percophis brasiliensis were found. Cannibalism has been observed.

眼镜蛇毒因子消耗补体对大鼠移植心急性排斥反应的抑制作用

目的 研究中华眼镜蛇毒因子(CVF)消耗补体对大鼠心脏移植急性排斥反应的影响.方法 以近交系BN大鼠为供者,Lewis大鼠为受者,建立腹腔异位心脏移植模型.实验分为2组,每组8只.CVF组:心脏移植术前3 d、2 d时,经受者尾静脉注射CVF 50 μg/kg;术前12 h至移植心停跳时,经尾静脉注射CVF 20 μg/kg,每2 d注射1次.对照组:术前、术后不给予受者任何特殊处理.术后观察移植心的存活时间,测定术后第1、3、5和6d及移植心停跳时的血清总补体活性(CH50法)、移植心C3沉积及CD3+T细胞浸润情况,并观察移植心的病理变化.结果 CVF组和对照组的移植心存活时间分别为(11.69±0.72)d和(6.65±0.35)d,两组比较,差异有统计学意义(P＜0.01).CVF组移植心组织内C3沉积和CD3+T细胞浸润程度均较对照组同期明显减轻,病理损害程度也较对照组同期明显减轻.结论 CVF消耗补体对大鼠心脏移植急性排斥反应起到了明显的抑制作用,从而延长移植心存活时间.

克洛曲对癌痛的双盲双模拟自身交叉研究

为了观察克洛曲在慢性癌痛病人中的疗效,采用多中心、双盲双模拟、自身交叉对照研究的方法, 比较了克洛曲片、曲马多胶囊和安慰剂对108 例中、重度癌痛的镇痛效果。结果表明克洛曲片的总有效率为 88. 0 % ,明显高于曲马多胶囊的72. 2 %( P < 0. 01) 和安慰剂的35. 2 %( P < 0. 001) ; 克洛曲片的缓解时间明 显延长( P < 0. 01) ; 克洛曲片的药物不良反应的发生率与曲马多胶囊相似, 但便秘的发生率明显低于曲马 多胶囊( P < 0. 05) 。克洛曲片对中、重度癌痛的镇痛效果确切,安全性较大。文章还对镇痛药临床研究的策 略方法及安慰剂效应进行了详细的讨论。