(Table 1) Geographical location and nutrient composition of saline lake sediments (SLS) and nearby adjacent biological soil crusts (BSC) identified as potential dust sources

While microbial communities of aerosols have been examined, little is known about their sources. Nutrient composition and microbial communities of potential dust sources, saline lake sediments (SLS) and adjacent biological soil crusts (BSC), from Southern Australia were determined and compared with a previously analyzed dust sample. Multivariate analyses of fingerprinting profiles indicated that the bacterial communities of SLS and BSC were different, and these differences were mainly explained by salinity. Nutrient concentrations varied among the sites but could not explain the differences in microbial diversity patterns. Comparison of microbial communities with dust samples showed that deflation selects against filamentous cyanobacteria, such as the Nostocales group. This could be attributed to the firm attachment of cyanobacterial filaments to soil particles and/or because deflation occurs mainly in disturbed BSC, where cyanobacterial diversity is often low. Other bacterial groups, such as Actinobacteria and the spore-forming Firmicutes, were found in both dust and its sources. While Firmicutes-related sequences were mostly detected in the SLS bacterial communities (10% of total sequences), the actinobacterial sequences were retrieved from both (11-13%). In conclusion, the potential dust sources examined here show highly diverse bacterial communities and contain nutrients that can be transported with aerosols. The obtained fingerprinting and sequencing data may enable back tracking of dust plumes and their microorganisms.

(Table 1) Age determination of sediment core BC5, Golf of Lion

Hitherto unknown abundance peaks of left coiling (l.c.) Neogloboquadrina pachyderma from a Gulf of Lions piston core indicate that abrupt cold spells associated with Atlantic Heinrich events affected the Mediterranean. N. pachyderma (l.c.) is typical of (sub) polar waters in the open ocean. The southern edge of its glacial North Atlantic bioprovince reached south Portugal. Only trace abundances of N. pachyderma (l.c.) are known from Quaternary Mediterranean sediments, suggesting that no significant "invasions" occured via the Strait of Gibraltar. The Gulf of Lions abundance peaks therefore seem to reflect area-specific thriving of a normally rare but indigenous taxon in the western Mediterranean through local favorable habitat development. The general planktonic foraminiferal record suggests that the basic hydrographic regime in the Gulf of Lions, with wintertime deep convective overturn, was relatively stable over the past 60 kyr. Under these conditions, high abundances of N. pachyderma (l.c.) would essentially imply temperature reductions of the order of 5°-8° relative to the present.

Mass accumulation rates of IODP Hole 320-U1333C

Understanding changes in export production through time provides insight into the response of the biological pump to global climate change, particularly during periods of rapid climate change. In this study we consider what role changes in export production may have had on carbon sequestration and how this may have contributed to the onset of the Eocene-Oligocene transition (EOT). In addition, we consider if these export production variations are dominantly controlled by orbitally driven climate variability. To accomplish these objectives, we report changes in export production in the Eastern Equatorial Pacific (EEP) from Site U1333 across the EOT reconstructed from a high-resolution record of marine barite accumulation rates (BAR). BAR fluctuations suggest synchronous declines in export production associated with the two-step increases in oxygen isotopes that define the transition. The reduction in productivity across the EOT suggests that the biological pump did not contribute to carbon sequestration and the cooling over this transition. We also report a previously undocumented peak in EEP export productivity before the EOT onset. This peak is consistent with export production proxies from the Southern Ocean, potentially implying a global driver for this precursor event. We propose that this enhanced export production and the associated carbon sequestration in the late Eocene may have contributed to the pCO2 drawdown at the onset of Antarctic glaciation.

Perspectivas del trabajo institucional de la policía nacional en relación al fenómeno del pandillismo en Bogotá. sector del Codito. (2004-2015-1)

El pandillismo es un fenómeno social que se presenta en las principales ciudades de Colombia desde hace varias décadas, sin embargo, solo en los últimos años el fenómeno a comenzado a acaparar mayor atención mediática e institucional. El pandillismo se ha convertido en una preocupación para la seguridad ciudadana, especialmente en las áreas urbanas. La Policía Nacional, en conjunto a Instituciones locales y nacionales ha venido realizando un trabajo focalizado para hacerle pie al fenómeno. En este trabajo se realizó una revisión histórica del pandillismo en Bogotá y un panorama continental. De la misma manera, se hace una descripción de los programas y estrategias que la Policía Nacional y las entidades públicas han realizado desde el año 2004 hasta el año 2015. Por último se realiza una evaluación del trabajo institucional enfocado en el sector del Codito, en la localidad de Usaquén.

Melhoria do Rastreamento e Prevenção do Câncer de Colo de Útero e do Câncer de Mama na ESF 1 Toríbio Veríssimo, Cruz Alta/RS

LAMELA CASTELLANOS, Estrella. Melhoria do rastreamento e prevenção do câncer de colo de útero e do câncer de mama na ESF1 Turíbio Veríssimo, município de Cruz Alta/RS. 2015. 78fls. Trabalho de Conclusão de Curso (Curso de Especialização em Saúde da Família)- Departamento de Medicina Social, Faculdade de Medicina, Universidade Federal de Pelotas, Pelotas, 2015. O Ministério da Saúde do Brasil adotou estratégias para a prevenção, detecção precoce mediante o rastreamento do câncer de colo de útero e da mama. Assim, realizamos na Estratégia de Saúde da Família Toribio Veríssimo, no município Cruz Alta /RS, entre os meses de abril a junho de 2015 uma intervenção em saúde, com o objetivo de melhorar o rastreamento e prevenção do câncer de colo de útero e do câncer de mama nas mulheres nas faixas etárias de 25 a 64 anos de idade e de 50 a 69 anos de idade da área de abrangência da unidade. Para a realização dessa intervenção, foram planejadas ações nos eixos de Monitoramento e Avaliação, Organização e Gestão do Serviço, Engajamento Público e Qualificação da Prática Clínica, levando-se em consideração as necessidades da população do território de abrangência, a viabilidade de aplicação das ações e a expectativa de obtenção de resultados. Foram realizadas capacitações a equipe de saúde, busca ativa e cadastramento das mulheres na comunidade e na unidade, avaliações de risco de câncer de mama, pesquisas de sinais de alerta do câncer de colo de útero, indicações de mamografias e exames citopatologicos (CP), ações educativas as usuárias tanto nas consultas individuais, como na sala de espera e de reuniões da unidade e na comunidade. Nos três meses da intervenção foram avaliados 78,0% (566) das 726 das mulheres na faixa etária de 25 a 64 anos de idade e 74,7% (239) das 320 mulheres na faixa etária de 50 a 69 anos idade, estimados pela planilha eletrônica de coleta de dados disponibilizada pelo curso de Especialização em Saúde da Família da Universidade Federal de Pelotas,Rio Grane do Sul. Os indicadores de qualidade foram alcançados para 100% das ações previstas a final da intervenção. Apesar da dificuldade encontradas ou longo da intervenção, com o trabalho em equipe e com o apoio da liderança da comunidade, conseguimos fazer um ótimo trabalho,e incorporar todas as ações do projeto às atividades diárias da unidade. Palavras-chave: Saúde da família; Atenção Primária à Saúde; Saúde da Mulher, Rastreamento de câncer de colo uterino e de mama

Up-regulation Of Dnam-1 And Nkp30, Associated With Improvement Of Nk Cells Activation After Long-term Culture Of Mononuclear Cells From Patients With Ovarian Neoplasia.

This study aimed at evaluating the functional activation and activating receptors expression on resting, short- and long-term NK and NK-like T cells from blood of ovarian neoplasia patients. Blood from patients with adnexal benign alterations (n = 10) and ovarian cancer (grade I-IV n = 14) were collected after signed consent. Effector cells activation was evaluated by the expression of the CD107a molecule. Short-term culture was conducted overnight with IL-2 and long-term culture for 21 days, by a method designed to expand CD56(+) lymphocytes. Short-term culture significantly increased NK cells activation compared to resting NK cells (p<0.05), however, the long-term procedure supported an even higher increase (p<0.001). Resting NK-like T cells showed poor activation, which was not altered by the culture procedures. The long-term culture effectively increased the expression of the activating receptors on NK and NK-like T cells, either by increasing the number of cells expressing a given receptor and/or by up-regulating their expression intensity. As a conclusion, the long-term culture system employed, resulted in a high number of functional NK cells. The culture system was particularly efficient on the up-regulation of NKp30 and DNAM-1 receptors on NK cells.

Hypertrophic Adenoid Is A Major Infection Site Of Human Bocavirus 1.

Human bocavirus 1 (HBoV1) is associated with respiratory infections worldwide, mainly in children. Similar to other parvoviruses, it is believed that HBoV1 can persist for long periods of time in humans, probably through maintaining concatemers of the virus single-stranded DNA genome in the nuclei of infected cells. Recently, HBoV-1 was detected in high rates in adenoid and palatine tonsils samples from patients with chronic adenotonsillar diseases, but nothing is known about the virus replication levels in those tissues. A 3-year prospective hospital-based study was conducted to detect and quantify HBoV1 DNA and mRNAs in samples of the adenoids (AD), palatine tonsils (PT), nasopharyngeal secretions (NPS), and peripheral blood (PB) from patients undergoing tonsillectomy for tonsillar hypertrophy or recurrent tonsillitis. HBoV1 was detected in 25.3% of the AD samples, while the rates of detection in the PT, NPS, and PB samples were 7.2%, 10.5%, and 1.7%, respectively. The viral loads were higher in AD samples, and 27.3% of the patients with HBoV had mRNA detectable in this tissue. High viral loads and detectable mRNA in the AD were associated with HBoV1 detection in the other sample sites. The adenoids are an important site of HBoV1 replication and persistence in children with tonsillar hypertrophy. The adenoids contain high HBoV1 loads and are frequently positive for HBoV mRNA, and this is associated with the detection of HBoV1 in secretions.

A Putative Otu Domain-containing Protein 1 Deubiquitinating Enzyme Is Differentially Expressed In Thyroid Cancer And Identifies Less-aggressive Tumours.

This study aimed to identify novel biomarkers for thyroid carcinoma diagnosis and prognosis. We have constructed a human single-chain variable fragment (scFv) antibody library that was selected against tumour thyroid cells using the BRASIL method (biopanning and rapid analysis of selective interactive ligands) and phage display technology. One highly reactive clone, scFv-C1, with specific binding to papillary thyroid tumour proteins was confirmed by ELISA, which was further tested against a tissue microarray that comprised of 229 thyroid tissues, including: 110 carcinomas (38 papillary thyroid carcinomas (PTCs), 42 follicular carcinomas, 30 follicular variants of PTC), 18 normal thyroid tissues, 49 nodular goitres (NG) and 52 follicular adenomas. The scFv-C1 was able to distinguish carcinomas from benign lesions (P=0.0001) and reacted preferentially against T1 and T2 tumour stages (P=0.0108). We have further identified an OTU domain-containing protein 1, DUBA-7 deubiquitinating enzyme as the scFv-binding antigen using two-dimensional polyacrylamide gel electrophoresis and mass spectrometry. The strategy of screening and identifying a cell-surface-binding antibody against thyroid tissues was highly effective and resulted in a useful biomarker that recognises malignancy among thyroid nodules and may help identify lower-risk cases that can benefit from less-aggressive management.