Exhaustive exercise causes an anti-inflammatory effect in skeletal muscle and a pro-inflammatory effect in adipose tissue in rats

It is well known that exhaustive exercise increases serum and skeletal muscle IL-6 concentrations. However, the effect of exhaustive exercise on the concentrations of other cytokines in the muscle and in the adipose tissue is controversial. The purpose of this study was to evaluate the effect of exhaustive exercise on mRNA and protein expression of IL-10, TNF-alpha and IL-6 in different types of skeletal muscle (EDL, soleus) and in two different depots of white adipose tissue (mesenteric-MEAT and retroperitoneal-RPAT). Rats were killed by decapitation immediately (E0 group, n = 6), 2 (E2 group, n = 6) and 6 (E6 group, n = 6) hours after the exhaustion protocol, which consisted of running on a treadmill (approximately 70% VO(2max) for 50 min and then subsequently at an elevated rate that increased at 1 m/min every minute, until exhaustion). The control group (C group, n = 6) was not subjected to exercise. Cytokine protein expression increased in EDL, soleus, MEAT and RPAT from all exercised groups, as detected by ELISA. EDL IL-10 and TNF-alpha expression was higher than that of the soleus. The IL-10/TNF-alpha ratio was increased in the skeletal muscle, especially in EDL, but it was found to be decreased in the adipose tissue. These results show that exhaustive exercise presents a different effect depending on the tissue which is analysed: in the muscle, it induces an anti-inflammatory effect, especially in type 2 fibres, while the pro-inflammatory effect prevails in adipose tissue, possibly contributing to increased lipolysis to provide energy for the exercising muscle.

Fluorimetric study of the pro-oxidant activity of EUK8 in the presence of hydrogen peroxide

The catalase mimetic complex Mn(III)-salen chloride (EUK8) was found to be pro-oxidant under low hydrogen peroxide concentrations. The increase in the fluorescence rate of the probe 1,2,3-dihydrorhodamine (DHR) in solution, as well as the carbonyl content of human serum albumin were found to be maximum at H(2)O(2):EUK8 molar ratios ranging from 0 to 2, supporting previous findings regarding the mechanism of EUK8 catalase activity and the formation of highly oxidative Mn(V)-O(2-) species. This pro-oxidant effect is precluded by the presence of glutathione. Cytotoxicity to HeLa cells, as probed by increased rate of oxidation of intracellular DHR, was not observed. Our findings suggest that the combination of H(2)O(2) and EUK8 at specific molar ratios, in the absence of reductants/antioxidants, induces the oxidation of organic molecules. It is shown that the fluorimetric determination of pro-oxidant activity of metal complexes is more sensitive than the colorimetric quantification of protein carbonyl content. The implications of our findings with respect to the somewhat confusing results arising from in vivo studies of EUK8 and other Mn(III) anti-oxidant metal complexes are discussed.

Teknik kontra användbarhet - vad påverkar utvecklingen? : En jämförelse av programmen Avid Media Composer 5.5 och Final Cut Pro X

Denna uppsats är en undersökning och en jämförelse av redigeringsprogrammen Avid Media Composer och Final Cut Pro X. Utgångspunkten i analysen är användbarhet och användaren. Saker som tas upp och diskuteras är teknikutvecklingen och konkurrensen mellan redigeringsprogrammen. Den historiska aspekten presenteras och förklaras för att lägga en grund till hur det ser ut i dagsläget. Några av de sakerna som jag kommer fram till är att teknikutvecklingen ofta sker på användbarhetens bekostnad och att användaren ofta måste anpassa sig efter tekniken. Konkurrensen mellan programmen påverkas av den växande marknaden för hobbyredigering och det leder till annorlunda utveckling.

Education and Earnings for Poverty Reduction : Short-Term Evidence of Pro-Poor Growth from the Mexican Oportunidades Program

Education, as an indispensable component of human capital, has been acknowledged to play a critical role in economic growth, which is theoretically elaborated by human capital theory and empirically confirmed by evidence from different parts of the world. The educational impact on growth is especially valuable and meaningful when it is for the sake of poverty reduction and pro-poorness of growth. The paper re-explores the precious link between human capital development and poverty reduction by investigating the causal effect of education accumulation on earnings enhancement for anti-poverty and pro-poor growth. The analysis takes the evidence from a well-known conditional cash transfer (CCT) program — Oportunidades in Mexico. Aiming at alleviating poverty and promoting a better future by investing in human capital for children and youth in poverty, this CCT program has been recognized producing significant outcomes. The study investigates a short-term impact of education on earnings of the economically disadvantaged youth, taking the data of both the program’s treated and untreated youth from urban areas in Mexico from 2002 to 2004. Two econometric techniques, i.e. difference-in-differences and difference-in-differences propensity score matching approach are applied for estimation. The empirical analysis first identifies that youth who under the program’s schooling intervention possess an advantage in educational attainment over their non-intervention peers; with this identification of education discrepancy as a prerequisite, further results then present that earnings of the education advantaged youth increase at a higher rate about 20 percent than earnings of their education disadvantaged peers over the two years. This result indicates a confirmation that education accumulation for the economically disadvantaged young has a positive impact on their earnings enhancement and thus inferring a contribution to poverty reduction and pro-poorness of growth.

Pro-premiär eller pro-president? : Om distinktionen mellan parlamentarism, presidentialism och semi-presidentialism

Propremiar or pro-president? On the distinction between parliamentarism, presitentialism and semi-presidentialism. From comparative research on the constitutional development in Central and Eastern Europe and also from the longstanding debate on whether parliamentarism or presidentialism best facilitates democracy, it is apparent that there has been and continues to be, a certain degree of confusion concerning the concepts of semi-presidentialism and presidentialism. Different scholars mean different things by the terms and therefore classify countries differently. In this article I argue that the conceptual dichotomy between pro-premiär (premier-presidentialism) and pro-president systems (presidentparliamentary systems) provide the best solution to several of the problems related to categorising constitutional types, most importantly perhaps to the presidential power dilemma. I, furthermore, employ these concepts on the post-communist constitutional systems and try to reveal patterns with regard to presidential power, geographical region and democratisation.

Pro-poor growth and social programmes in Brazil

From a methodological point of view, this paper makes two contributions to the literature. One contribution is the proposal of a new measure of pro-poor growth. This new measure provides the linkage between growth rates in mean income and in income inequality. In this context, growth is defined as propoor (or anti-poor) if there is a gain (or loss) in the growth rate due to a decrease (or increase) in inequality. The other contribution is a decomposition methodology that explores linkages between growth patterns and social policies. Through the decomposition analysis, we assess the contribution of different income sources to growth patterns. The proposed methodologies are then applied to the Brazilian National Household Survey (PNAD) covering the period 1995-2004. The paper analyzes the evolution of Brazilian social indicators based on per capita income exploring links with adverse labour market performance and social policy change, with particular emphasis on the expansion of targeted cash transfers and devising more pro-poor social security benefits.

A gestão em vara judicial sob a visão do juiz pro ativo

O modelo de gestão inovador mostrou que é possível fazer um ambiente de excelência onde o Poder Judiciário seja reconhecido, respeitado e confiável aos jurisdicionados, na medida em que se assegura uma prestação jurisdicional efetiva num espaço de tempo razoável, garantindo legitimidade e credibilidade às suas decisões, sob a visão de um juiz proativo, com objetivos estratégicos pré-definidos, sob um olhar idealizador, uma equipe integrada, motivada e comprometida. O modelo de gestão inovador foi experimentado na Vara do Juizado Especial Cível da Comarca de Jaru, no Estado de Rondônia, onde se procurou conferir uma rotina lógico-jurídica ao fluxo processual, sem prejuízo da qualidade, e em com total harmonia aos postulados normativos do Juizado Especial Cível e as regras constitucionais prescritas.

Obtenção e avaliação da proteína BYC (Boophilus Yolk pro-Cathepsin) recombinante em uma vacina contra o carrapato Boophilus microplus

O carrapato Boophilus microplus é um ectoparasita hematófago que infesta os rebanhos bovinos de regiões tropicais e subtropicais, causando grande prejuízo à pecuária. O principal método de controle deste parasita baseia-se no uso de acaricidas, entretanto, o uso de vacinas tem sido estudado como um método de controle promissor. A Boophilus Yolk pro-Cathepsin (BYC) é uma aspártico proteinase presente no ovo do carrapato e envolvida na embriogênese que foi anteriormente testada como imunógeno vacinal. Neste estudo, o cDNA da BYC foi amplificado por PCR e clonado em dois vetores de expressão para produção de duas formas da proteína recombinante com cauda de histidina, rBYC e rBYC-Trx (fusionada com tioredoxina). As duas formas foram expressas em Escherichia coli na forma de corpúsculos de inclusão (CI) e comparadas quanto ao nível de expressão, solubilidade e rendimento na purificacão. Três agentes desnaturantes (N-lauroil sarcosina, hidrocloreto de guanidina e uréia) foram testados para solubilização dos CIs. Sarcosina foi o agente mais eficiente, solubilizando mais de 90 % de rBYC-Trx e rBYC. As duas proteínas recombinantes foram purificadas em cromatografia de afinidade por metal (Ni2+), sob condições desnaturantes. O rendimento na purificação da proteína solúvel foi de 84 % para r-BYC-Trx e 6 % para rBYC. As duas formas foram reconhecidas por soro de coelhos, camundongos e bovinos previamente imunizados com BYC nativa, demonstrando a existência de epítopos comuns entre a BYC nativa e as formas recombinantes expressas em E. coli. Para verificar o potencial vacinal da proteína recombinante, um grupo de bovinos Hereford foi imunizado com rBYC e desafiado com 20.000 larvas de B. microplus por animal. Os soros dos bovinos imunizados reconheceram a BYC nativa em ELISA e “Western blot”, com títulos entre 500 e 4.000. Os resultados do desafio mostraram uma proteção parcial contra a infestação, com 25 % de proteção global. O perfil de expressão de citocinas (IL-2, IL-4, IL-10, IFN-γ) foi verificado por RT-PCR, porém os resultados não permitiram identificar a polarização da resposta imune em Th1 ou Th2. Os resultados de imunoproteção obtidos com a BYC recombinante foram similares aos obtidos na imunização de bovinos com BYC nativa, indicando a possibilidade de uso da forma recombinante como imunógeno vacinal.

Pro- and anti-market reforms in democratic Brazil

Instituto Brasileiro de Economia

Use of the lean model at Pro Sky

O modelo lean startup representa uma forma recente de desenvolver novos produtos e serviço. No entanto, existe pouca informação sobre a implementação em empresas. O autor desta tese aborda esta lacuna na literatura, fornecendo um exemplo prático de uma organização adaptando o modelo lean startup. O estudo de caso único envolve o intermediário de serviços aéreos Pro Sky que tenta desenvolver um novo serviço de concierge em aeroporto. Um entendimento profundo é obtido por meio de entrevistas, participant-observation e a simulação do novo serviço. Em comparação com desenvolvimentos passados de novos serviços na Pro Sky, o modelo lean startup ajuda a acelerar o processo de desenvolvimento, reduzir o desperdício de recursos e reduzir os efeitos negativos dos vieses cognitivos. O modelo reforça o objetivo da Pro Sky se desenvolver numa empresa voltada para o cliente. Obstáculos incluem oferecer aos clientes um produto inacabado e o medo resultante da perda de reputação.

Linkages between pro-poor growth, social programmes and labour market : the recent Brazilian experience

From a methodological point of view, this paper makes two contlibutions to the literature. One contribution is the proposal of a new measure of pro-poor growth. This new measure provides the linkage between growth rates in mean income and in income inequality. In this context, growth is defined as pro-poor (or anti-poor) if there is a gain (or loss) in the growth rate due to a decrease (or increase) in inequality. The other contribution is a decomposition methodology that explores linkages between three dimensions: growth pattems, labour market performances. and social policies. Through the decomposition analysis, growth in per capita income is explained in terms of four labour market components: the employment rate. hours of work, the labour force participation rate. and productivity. We also assess the contribution of different nonlabour income sources to growth patterns. The proposed methodologies are then applied to the Brazilian National Household Survey (PNAD) covering the period 1995-2004. The paper analyzes the evolution of Brazilian social indicators based on per capita income exploring links with adverse labour market performance and social policy change, with particular emphasis on the expansion of targeted cash transfers and devising more propoor social security benefits.

Elementos de confiança e cultura organizacional que dificultam a gestão pro processos na área de tecnologia da informação e telecomunicações de uma empresa de energia

Este estudo é dedicado à compreensão dos fatores culturais e de confiança que dificultam o alinhamento por processos na área de Tecnologia da Informação e Telecomunicações de uma empresa de economia mista. Visa compreender e traduzir o modo como a cultura dessa organização impacta nos resultados alcançados pelo modelo de gestão por processos vigente. Fazendo uso de referencial teórico das áreas de estudo de cultura organizacional, cultura nacional e confiança nas organizações, este estudo buscou alinhar estes conhecimentos por meio de um viés ainda pouco explorado no ambiente acadêmico. Aqui fizemos uso do conceito de cultura como um meio e não como um fim em si, associando classificação da cultura em dimensões, os traços da cultura brasileira e os estudos de “confiança” numa mescla ordenada de conceitos capaz de nos responder à questão de pesquisa. Esta pesquisa representa o resultado de um esforço de imersão nas rotinas da organização e descrição dos seus elementos culturais por meio de observação e entrevistas no ambiente estudado. Por fim, este trabalho se destina a contribuir ao aprimoramento dos processos para atingir melhores resultados na TIC.

Profiling allergic asthma volatile metabolic patterns using a headspace-solid phase microextraction/gas chromatography based methodology

Allergicasthmarepresentsanimportantpublichealthissuewithsignificantgrowthovertheyears,especially in the paediatric population. Exhaled breath is a non-invasive, easily performed and rapid method forobtainingsamplesfromthelowerrespiratorytract.Inthepresentmanuscript,themetabolicvolatile profiles of allergic asthma and control children were evaluated by headspace solid-phase microextraction combined with gas chromatography–quadrupole mass spectrometry (HS-SPME/GC–qMS). The lack ofstudiesinbreathofallergicasthmaticchildrenbyHS-SPMEledtothedevelopmentofanexperimental design to optimize SPME parameters. To fulfil this objective, three important HS-SPME experimental parameters that influence the extraction efficiency, namely fibre coating, temperature and time extractions were considered. The selected conditions that promoted higher extraction efficiency corresponding to the higher GC peak areas and number of compounds were: DVB/CAR/PDMS coating fibre, 22◦C and 60min as the extraction temperature and time, respectively. The suitability of two containers, 1L Tedlar® bags and BIOVOC®, for breath collection and intra-individual variability were also investigated. The developed methodology was then applied to the analysis of children exhaled breath with allergicasthma(35),fromwhich13hadalsoallergicrhinitis,andhealthycontrolchildren(15),allowing to identify 44 volatiles distributed over the chemical families of alkanes (linear and ramified) ketones, aromatic hydrocarbons, aldehydes, acids, among others. Multivariate studies were performed by Partial LeastSquares–DiscriminantAnalysis(PLS–DA)usingasetof28selectedmetabolitesanddiscrimination between allergic asthma and control children was attained with a classification rate of 88%. The allergic asthma paediatric population was characterized mainly by the compounds linked to oxidative stress, such as alkanes and aldehydes. Furthermore, more detailed information was achieved combining the volatile metabolic data, suggested by PLS–DA model, and clinical data.