Die Kultur des Pfirsichbaumes am Spaliere. Für Gärtner, Gartenbesitzer und Freunde des Pfirsichbaumes.

Mode of access: Internet.

The gardener /

v. 1. The potato, cucumber, gooseberry, strawberry, and dahlia -- v. 2. The auricula, asparagus, and pine apple -- v. 3. The peach and grape vine.

1916 University of Michigan Football Team

University of Michigan Football Team, 1916

Back Row: R. Glenn Dunn, Fred Rehor, Elton Wieman, Maurice Dunne, Hoyne Howe, Clarence Skinner, Cedric Smith, James Sharpe, James Whalen, Frank Willard, Earl MacLaughlin, Orva Williams, Philip Raymond, Sidney Eggert, Henry Dieters

Middle Row: trainer Harry Tuthill, asst. coach Miller Pontius, Harry McCallum, Richard Weske, Joseph Hanish, Walter Nieman, Albert Martens, Clifford Gracey, Willard Peach, Alan Boyd, John Goodsell

Nathaniel (?) Robbins, Donald Bathrick, N.J. Brazell, Edward Biber, Clifford Sparks, coach Fielding Yost, captain John Maulbetsch, Owen Watts, Harold Ziegler, asst. coach Prentiss Douglass, Charles Beath

1999 Ice Hockey Team, posed with CCHA Playoff Trophy

Top Row: student trainer Jarrett Mason, student equip. mngr. Pat Fisher, equip. mngr. Ian Hume, student equip. mngr. Jeff Krzeszak, video coordinator Josh Richelow

Third Row: Robbie Kohen, Jeff Jillson, Jay Vancik, Geoff Koch, Scott Crawford, Kevin Magnuson, Josh Langfeld, Dave Huntzicker

Second Row: Kevin O'Malley, Scott Matzka, Bill Trainor, Sean Peach, Andrew Merrick, Mike Comrie, Bob Gassoff, Mark Kosick, Krikor Arman, Craig Murray, Josh Blackburn

Front Row: asst. coach Billy Powers, Greg Daddario, Greg Crozier, Bobby Hayes, head coach Red Berenson, Bubba Berenzweig, Dale Rominski, Justin Clark, Sean Ritchlin, asst. coach Mel Pearson

1999 Ice Hockey Team

Top Row: trainer Rick Bancroft, student trainer Jarrett Mason, student equip. mngr. Pat Fisher, student equip. mngr. Jeff Krzeszak, video coordinator Josh Richelew, student equip. mngr. David Brooks, equip. mngr. Ian Hume

Third Row: Jay Vancik, Jeff Jillson, Geoff Koch, Krikor Arman, Andrew Merrick, Dave Huntzicker, Mike Van Ryn, Josh Langfeld

Second Row: Kevin O'Malley, Scott Matzka, Bill Trainor, Sean Peach, Scott Crawford,Mike Comrie, Mark Kosick, Bob Gassoff, Craig Murray, Kevin Magnuson Josh Blackburn

Front Row: asst. coach Billy Powers, Greg Daddario, Greg Crozier, Bobby Hayes, head coach Red Berenson, Bubba Berenzweig, Dale Rominski, Justin Clark, Sean Ritchlin, asst. coach Mel Pearson

1916 UM Football Team, posed on Club House steps

Back Row: R. Glenn Dunn, Fred Rehor, Elton Wieman, Maurice Dunne, Hoyne Howe, Clarence Skinner, Cedric Smith, James Sharpe, James Whalen, Frank Willard, Earl MacLaughlin, Orva Williams, Philip Raymond, Sidney Eggert, Henry Dieters

Middle Row: trainer Harry Tuthill, asst. coach Miller Pontius, Harry McCallum, Richard Weske, Joseph Hanish, Walter Nieman, Albert Martens, Clifford Gracey, Willard Peach, Alan Boyd, John Goodsell

Nathaniel (?) Robbins, Donald Bathrick, N.J. Brazell, Edward Biber, Clifford Sparks, coach Fielding Yost, captain John Maulbetsch, Owen Watts, Harold Ziegler, asst. coach Prentiss Douglass

Shui mi tao pu /

Imprint date from preface.

Pratique raisonnée de la taille du pêcher : contenant sa culture, sa multiplication, les principes généraux de la taille et leur application aux formes les plus usitées, les moyens de restaurer les arbres et de rémédier aux maladies et accidents dont le pêcher peut être frappé, et la description des variétés de pêches les meilleires ǎ cultiver /

Mode of access: Internet.

A series of familiar discourses for every Sunday and festival of the year /

Errata at end of both volumes.

Identity and pathogenicity of Fusarium spp. Isolated from wheat fields in Queensland and northern New South Wales

To establish the identity of Fusarium species associated with head blight (FHB) and crown rot (CR) of wheat, samples were collected from wheat paddocks with different cropping history in southern Queensland and northern New South Wales during 2001. CR was more widespread but FHB was only evident in northern NSW and often occurred with CR in the same paddock. Twenty different Fusarium spp. were identified from monoconidial isolates originating from different plant parts by using morphology and species-specific PCR assays. Fusarium pseudograminearum constituted 48% of all isolates and was more frequently obtained from the crown, whereas Fusarium graminearum made up 28% of all isolates and came mostly from the head. All 17 Fusarium species tested caused FHB and all 10 tested caused CR in plant infection assays, with significant (P < 0.001) difference in aggressiveness among species and among isolates within species for both diseases. Overall, isolates from stubble and crown were more aggressive for CR, whereas isolates from the flag leaf node were more aggressive for FHB. Isolates that were highly aggressive in causing CR were those originating from paddocks with wheat following wheat, whereas those from fields with wheat following maize or sorghum were highly aggressive for FHB. Although 20% of isolates caused severe to highly severe FHB and CR, there was no significant (P < 0.32) correlation between aggressiveness for FHB and CR. Given the ability of F. graminearum to colonise crowns in the field and to cause severe CR in bioassays, it is unclear why this pathogen is not more widely distributed in Australia.

Genetic diversity of Australian Fusarium graminearum and F-pseudograminearum

Genotypic diversity in Fusarium pseudograminearum and F. graminearum from Australia and the relationship between diversity and pathogen aggressiveness for head blight and/or crown rot of wheat were examined. Amplified fragment length polymorphism (AFLP) analysis revealed a high level of genotypic diversity within each species. Sixty-three of the 149 AFLP loci were significantly different between the two species and 70 of 72 F. pseudograminearum and 56 of 59 F. graminearum isolates had distinct haplotypes. When head blight and crown rot severity data from a recently published work on isolates representing the entire range of aggressiveness were used, only the genotypic diversity of F. pseudograminearum was significantly associated with its aggressiveness for the two diseases. Cluster analyses clearly demonstrated the polyphyletic structures that exist in both pathogen populations. The spatial diversity within F. graminearum was high within a single field, while frequent gene flow (N-m similar to 14) and a low fixation index (G(st) = 0.03) were recorded among F. pseudograminearum isolates from the adjacent states of New South Wales and Queensland. The differences in population structure between the heterothallic F. pseudograminearum (teleomorph G. coronicola) and the homothallic F. graminearum (teleomorph G. zeae) were not as pronounced as expected given their contrasting mating systems. Neither species was panmictic or strictly clonal. This points to sexual recombination in F. pseudograminearum, suggesting that ascospores of G. coronicola may also play a role in its biology and epidemiology.

Different routes to pure food allergens: extraction, recombinant techniques and total chemical synthesis for obtaining the plant-food pan-allergen LTP

La richiesta di allergeni puri è in continuo aumento per scopi diagnostici, come standard per metodi di rilevamento e di quantificazione, per l'immunoterapia e per lo studio a livello molecolare dei meccanismi delle reazioni allergiche, al fine di facilitare lo sviluppo di possibili cure. In questa tesi di dottorato sono descritte diverse strategie per l’ottenimento di forme pure di non-specific Lipid Transfer Proteins (nsLTPs), le quali sono state riconosciute essere rilevanti allergeni alimentari in molti frutti e verdure comunemente consumati e sono state definite come modello di veri allergeni alimentari. Una LTP potenzialmente allergenica, non nota in precedenza, è stata isolata dalle mandorle, mentre una LTP dall’allergenicità nota contenuta nelle noci è stata prodotta mediante tecniche di DNA ricombinante. Oltre a questi approcci classici, metodi per la sintesi chimica totale di proteine sono stati applicati per la prima volta alla produzione di un allergene, utilizzando Pru p 3, la LTP prototipica e principale allergene della pesca nell'area mediterranea, come modello. La sintesi chimica totale di proteinepermette di controllarne completamente la sequenza e di studiare la loro funzione a livello atomico. La sua applicazione alla produzione di allergeni costituisce perciò un importante passo avanti nel campo della ricerca sulle allergie alimentari. La proteina Pru p 3 è stata prodotta nella sua intera lunghezza e sono necessari solo due passaggi finali di deprotezione per ottenere il target nella sua forma nativa. Le condizioni sperimentali per tali deprotezioni sono state messe a punto durante la produzione dei peptidi sPru p 3 (1-37) e sPru p 3 (38-91), componenti insieme l'intera proteina. Tecniche avanzate di spettrometria di massa sono state usate per caratterizzare tutti i composti ottenuti, mentre la loro allergenicità è stata studiata attraverso test immunologici o approcci in silico.

The tear film and contact lens wear

Contact lenses have become a popular method of vision correction for millions of people globally. As with all devices designed for use within the body, interactions occur between the implanted material and the surrounding biological fluid. A common complaint of lens wearers is that they often experience symptoms of dry eye whilst wearing lenses. This sensation is often heightened towards the end of the day. Through the course of this study, various analytical techniques have been utilised including one dimensional electrophoresis and Western Blotting to study the protein profiles of tear samples. By studying the tears of non-contact lens wearers, it was possible to analyse what could be considered normal, healthy, individuals. A clinical study was also undertaken which followed a population of individuals from the neophyte stage to one whereby they were accustomed lens wearers. Tears were monitored at regular intervals throughout the course of this study and worn contact lenses were also analysed for proteins that had been deposited both on and within the lens. Contact lenses disrupt the tear film in a physical manner by their very presence. They are also thought to cause the normal protein profile to deviate from what would be considered normal. The tear film deposits proteins and lipids onto and within the lens. The lens may therefore be depriving the tear film of certain necessary components. The ultimate aim of this thesis was to discover how, and to what extent, lenses affected tear proteins and if there were any proteins in the tear fluid that had the potential to be used as biochemical markers. Should this be achievable it may be possible to identify those individuals who were more likely to become intolerant lens wearers. This study followed the changes taking place to the tear film as an effect of wearing contact lenses. Twenty-eight patients wore two different types of silicone hydrogel lenses in both a daily wear and a continuous wear regime. The tear protein profiles of the lens-wearers were compared with a control group of non-lens wearing individuals. The considerable amount of data that was generated enabled the clearly observable changes to the four main tear proteins to be monitored.