Displasia neuronal intestinal: análise de critérios morfológicos e comparação de métodos diagnósticos

Pós-graduação em Patologia - FMB

Importância dos mastócitos na patogênese da distrofia muscular de Duchenne em cães Golden Retriever

Pós-graduação em Medicina Veterinária - FCAV

Infecção por strigea falconis em buteo magnirostris no Brasil

Background: Buteo magnirostris, popularly known as roadside hawk belongs to the family Accipitridae, Ciconiiformes. The specimen is common throughout Brazil inhabiting open areas, tolerating disturbed areas very well, but avoiding dense forests. The trematodes are common parasites in the intestines of birds of prey, with scant notice of pathogenic infections. However, severe infections of trematodes Strigeidae family have been previously reported as a cause of anemia, diarrhea, enteritis, weight loss and death. This paper aims to report the occurrence of infection in S. Falconis in B. magnirostris diagnosed by post mortem examination. Case: The specimen of B. magnirostris, male, young was sent for necropsy at the Laboratory of Veterinary Pathology, Federal Rural University of Semi-Arid (UFERSA), Mossoró-RN, Brazil. With a history of apathy, anorexia, diarrhea and death in one course of 24 h. The free bird life and even puppy had been captured for training and practice of falconry shortly before the clinical manifestation of infection (time of captivity uninformed). On physical examination ruffled feathers, cachexia and pallor of skeletal muscle was observed. At necropsy there was severe enteritis with petechiae and accumulation of liquid contents into the duodenum. Fifty-two trematodes were found set in duodenal mucosa. The other organs and structures showed no changes. Fragments of all organs were harvested, fixed in 10% formalin buffered, routinely processed for histopathology and stained with hematoxylin and eosin (HE). Parasites were carefully collected, washed in saline, fixed, processed and identified according to the morphology and taxonomy. Histologically, the lesions were restricted to the duodenum and were characterized by melting, severe atrophy and necrosis of the epithelial cells of the intestinal villi; inflammatory infiltrate (consisting of lymphocytes, plasma cells and eosinophils) in the lamina propria, in addition to trematodes infiltrated the mucosa and lamina propria. These were 60-80 mm in diameter, consisted of parenchymal body enclosed by the integument. In some cross sections of the parasite was possible to observe the presence of cecum, testis and uterus, with some variations between sections; there were also yellowish eggs and coated with a delicate membrane. The trematodes contain approximately 1 mm in length and used as morphology and taxonomy has been identified as S. Falconis. Discussion: S. Falconis is a trematode intestinal parasite of birds of prey, with reports of its occurrence in Europe, North America and Central. In neotropical regions is described the occurrence of the subspecies S. Falconis brasiliana. Although the absence of clinical signs is a common pattern, parasitism by trematodes may become evident, common to captivity stress conditions, and thus infections, even for low pathogenic parasites can cause diarrhea, anorexia, weight loss and death, as reported in this paper. A factor that possibly contributed to the scant notice is its small size, which makes the observation of this parasite in analysis of necropsy in non-pathological conditions and also not familiar with the technical laboratories in the morphological shape of the eggs, which creates difficulty in finding the parasite in parasitological analysis in captive animals. Despite being considered poorly pathogenic trematodes, epidemiologically, the presence of the parasite should be considered a health risk to free-living predators, newly captive in parks, zoos, veterinary hospitals, triage center for wildlife and creators, as they may express pathogenicity in immunosuppressed animals. This work contributes to recording the presence S. falconis parasitizing the duodenal mucosa of B. magnirostris in Brazil.

Toxicidade de cádmio e zinco em Danio rerio: comparação entre valores permitidos em legislação para proteção da vida aquática e a potencial atuação como interferentes endócrinos

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Influência da hipertensão arterial no padrão fenotípico da lesão periapical, na diferenciação dos osteoclastos, na resposta teciduale na capacidade de mineralização do cimento reparador MTA

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Efeito da inibição da NADPH oxidase sobre o estresse oxidativo e o fenótipo muscular esquelético de ratos com insuficiência cardíaca crônica induzida por estenose aórtica

Pós-graduação em Fisiopatologia em Clínica Médica - FMB

Influência do consumo crônico de diferentes concentrações de álcool nos tecidos periodontais e na evolução da periodontite experimental

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Obtenção e caracterização de populações de células-tronco CD200 e CD34 positivas da pele na espécie canina

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Estudo morfológico do efeito radioprotetor da vitamina E (dl-alfa-tocoferil) na reparação tecidual em ratos

Esta pesquisa teve por finalidade avaliar a ação da vitamina E como radioprotetora no processo de reparação tecidual em ratos, após sofrerem um procedimento cirúrgico, que consistiu da produção de uma ferida na região dorsal anterior. Os animais foram divididos em cinco grupos: grupo CO (controle) - constituído de animais em que foi produzida somente a ferida; grupo VE - pré-tratamento com vitamina E (90 UI); grupo IR - irradiação três dias após a cirurgia; grupo VEIR - pré-tratamento com 90 UI de vitamina E e irradiação de suas bordas três dias após a cirurgia; grupo OIR - pré-tratamento com óleo de oliva e irradiação de suas bordas três dias após a cirurgia. A ação radioprotetora da vitamina E foi avaliada pela coloração por hematoxilina-eosina para análise morfológica do tecido de granulação, aos 4, 7, 14 e 21 dias após a cirurgia. A análise dos resultados mostrou que o retardo no processo de reparação tecidual causado por 6 Gy de radiação de elétrons com feixe de 6 MeV não ocorreu no grupo de animais que recebeu vitamina E, mostrando-se esta substância efetiva como radioprotetora.

Efeito da inibição da NADPH oxidase sobre o estresse oxidativo e o fenótipo muscular esquelético de ratos com insuficiência cardíaca crônica induzida por estenose aórtica

Pós-graduação em Fisiopatologia em Clínica Médica - FMB

Influência do consumo crônico de diferentes concentrações de álcool nos tecidos periodontais e na evolução da periodontite experimental

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Obtenção e caracterização de populações de células-tronco CD200 e CD34 positivas da pele na espécie canina

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

The effects of conjugated estrogen, raloxifene and soy extract on collagen in rat bones

Objective To evaluate the action of conjugated equine estrogen, raloxifene and isolated or combined genistein-rich soy extracts on collagen fibers in the bones of oophorectomized rats. Materials and methods Seventy female rats received testosterone propionate (0.1 mu g/g) on the 9th day after birth. At 6 months of age, the rats were administered the vehicle (propylene glycol, 0.5 ml/day), and ten of the rats were randomly chosen to comprise the non-oophorectomized control group (GI). The other 60 rats were ovariectomized and randomized into six groups of ten as follows: GII, vehicle; GIII, conjugated equine estrogen (CEE), 50 mu g/kg/day; GIV, raloxifene (RAL), 0.75 mg/kg/day; GV, genistein-rich soy extract (GSE), 300 mg/kg/day; GVI, CEE + GSE, 50 mu g/kg/day + 300 mg/kg/day; and GVII, CEE + RAL, 50 mu g/kg/day + 0.75 mg/kg/day. Three months after surgery, the drugs were administered for 60 consecutive days. All rats were euthanized, and their left tibiae were removed for histological routine. The histological sections were stained with hematoxylin-eosin, and picrosirius for evaluating bone microarchitecture. Types I and II collagen fibers were analyzed by immunofluorescence. Data analysis was carried out with ANOVA and Tukey's test. Results Collagen reduction was significant in the GIII animals when compared to the other groups (p < 0.05). There was no significant difference in the thickness of collagen fibers among the groups. There was a greater quantity of type III collagen in GVI than in the other groups. Conclusion Our data indicate that conjugated equine estrogen improves bone quality because it increases the quantity of type I collagen while reducing the quantity of thin collagen fibers. In addition, the combination of CEE and raloxifene or genistein-rich soy extract is not as efficient as CEE itself to improve bone quality.

GaAs 904-nm laser irradiation improves myofiber mass recovery during regeneration of skeletal muscle previously damaged by crotoxin

This work investigated the effect of gallium arsenide (GaAs) irradiation (power: 5 mW; intensity: 77.14 mW/cm(2), spot: 0.07 cm(2)) on regenerating skeletal muscles damaged by crotoxin (CTX). Male C57Bl6 mice were divided into six groups (n = 5 each): control, treated only with laser at doses of 1.5 J or 3 J, CTX-injured and, CTX-injured and treated with laser at doses of 1.5 J or 3 J. The injured groups received a CTX injection into the tibialis anterior (TA) muscle. After 3 days, TA muscles were submitted to GaAs irradiation at doses of 1.5 or 3 J (once a day, during 5 days) and were killed on the eighth day. Muscle histological sections were stained with hematoxylin and eosin (H&E) in order to determine the myofiber cross-sectional area (CSA), the previously injured muscle area (PIMA) and the area density of connective tissue. The gene expression of MyoD and myogenin was detected by real-time PCR. GaAs laser at a dose of 3 J, but not 1.5 J, significantly increased the CSA of regenerating myofibers and reduced the PIMA and the area density of intramuscular connective tissue of CTX-injured muscles. MyoD gene expression increased in the injured group treated with GaAs laser at a dose of 1.5 J. The CTX-injured, 3-J GaAs laser-treated, and the CTX-injured and treated with 3-J laser groups showed an increase in myogenin gene expression when compared to the control group. Our results suggest that GaAs laser treatment at a dose of 3 J improves skeletal muscle regeneration by accelerating the recovery of myofiber mass.

Histomorphometric analysis of the effects of creatine on rat myometrium

Objective: To analyze the myometrial thickness of rats subjected to creatine (Cr) ingestion. Study design: A total of 14 rats was equally divided into the control group (ConGr) receiving 1 ml potable water and the creatine group (CrGr) subjected to the ingestion of 1.6 g/kg Cr diluted in 1 ml potable water. At the end of 8 weeks, the animals were anesthetized (xylazine and ketamine) and sacrificed, the uteri and ovaries stained with hematoxylin and eosin, the thickness of both the myometrium and the epithelium measured and the follicles counted. Results: Analysis revealed a significant increase in thickness of the myometrium in the CrGr (272.26 +/- 66.71 mu m) contrasted with that from the ConGr (160.76 +/- 35.65 mu m), CrGr > ConGr (p < 0001). Conclusion: Our data suggest that Cr changed myometrial morphology in rats by enhancing myometrial thickness, but its action mechanism in the smooth muscle is still unclear.