Biology and population dynamics of cowcod (Sebastes levis) in the southern California Bight

Cowcod (Sebastes levis) is a large (100-cm-FL), long-lived (maximum observed age 55 yr) demersal rockfish taken in multispecies commercial and recreational fisheries off southern and central California. It lives at 20–500 m depth: adults (>44 cm TL) inhabit rocky areas at 90–300 m and juveniles inhabit fine sand and clay at 40–100 m. Both sexes have similar growth and maturity. Both sexes recruit to the fishery before reaching full maturity. Based on age and growth data, the natural mortality rate is about M =0.055/yr, but the estimate is uncertain. Biomass, recruitment, and mortality during 1951–98 were estimated in a delay-difference model with catch data and abundance indices. The same model gave less precise estimates for 1916–50 based on catch data and assumptions about virgin biomass and recruitment such as used in stock reduction analysis. Abundance indices, based on rare event data, included a habitat-area–weighted index of recreational catch per unit of fishing effort (CPUE index values were 0.003–0.07 fish per angler hour), a standardized index of proportion of positive tows in CalCOFI ichthyoplankton survey data (binomial errors, 0–13% positive tows/yr), and proportion of positive tows for juveniles in bottom trawl surveys (binomial errors, 0–30% positive tows/yr). Cowcod are overfished in the southern California Bight; biomass during the 1998 season was about 7% of the virgin level and recent catches have been near 20 metric tons (t)/yr. Projections based on recent recruitment levels indicate that biomass will decline at catch levels > 5 t/yr. Trend data indicate that recruitment will be poor in the near future. Recreational fishing effort in deep water has increased and has become more effective for catching cowcod. Areas with relatively high catch rates for cowcod are fewer and are farther offshore. Cowcod die after capture and cannot be released alive. Two areas recently closed to bottom fishing will help rebuild the cowcod stock.

Reproductive biology of the blue swimmer crab (Portunus pelagicus, Decapoda: Portunidae) in five bodies of water on the west coast of Australia

Portunus pelagicus was collected at regular intervals from two marine embayments and two estuaries on the lower west coast of Australia and from a large embayment located approximately 800 km farther north. The samples were used to obtain data on the reproductive biology of this species in three very different environments. Unlike females, the males show a loosening of the attachment of the abdominal flap to the cephalothorax at a prepubertal rather than a pubertal molt. Males become gonadally mature (spermatophores and seminal fluid present in the medial region of the vas deferentia) at a very similar carapace width (CW) to that at which they achieve morphometric maturity, as reflected by a change in the relative size of the largest cheliped. Logistic curves, derived from the prevalence of mature male P. pelagicus, generally had wider confidence limits with morphometric than with gonadal data. This presumably reflects the fact that the morphometric (allometric) method of classifying a male P. pelagicus as mature employs probabilities and is thus indirect, whereas gonadal structure allows a mature male to be readily identified. However, the very close correspondence between the CW50’s derived for P. pelagicus by the two methods implies that either method can be used for management purposes. Portunus pelagicus attained maturity at a significantly greater size in the large embayment than in the four more southern bodies of water, where water temperatures were lower and the densities of crabs and fishing pressure were greater. As a result of the emigration of mature female P. pelagicus from estuaries, the CW50’s derived by using the prevalence of mature females in estuaries represent overestimates for those populations as a whole. Estimates of the number of egg batches produced in a spawning season ranged from one in small crabs to three in large crabs. These data, together with the batch fecundities of different size crabs, indicate that the estimated number of eggs produced by P. pelagicus during the spawning season ranges from about 78,000 in small crabs (CW=80 mm) to about 1,000,000 in large crabs (CW=180 mm).

Age and size composition, growth rate, reproductive biology, and habitats of the West Australian dhufish (Glaucosoma hebraicum) and their relevance to the management of this species

Samples of the commercially and recreationally important West Australian dhufish (Glaucosoma hebraicum) were obtained from the lower west coast of Australia by a variety of methods. Fish <300 mm TL were caught over flat, hard substrata and low-lying limestone reefs, whereas larger fish were caught over larger limestone and coral reef formations. Maximum total lengths, weights, and ages were 981 mm, 15.3 kg, and 39 years, respectively, for females and 1120 mm, 23.2 kg, and 41 years, respectively, for males. The von Bertalanffy growth curves for females and males were significantly different. The values for L∞, k, and t0 in the von Bertalanffy growth equations were 929 mm, 0.111/year, and –0.141 years, respectively, for females, and 1025 mm, 0.111/year, and –0.052 years, respectively, for males. Preliminary estimates of total mortality indicated that G. hebraicum is now subjected to a level of fishing pressure that must be of concern to fishery managers. Glaucosoma hebraicum, which spawns between November and April and predominantly between December and March, breeds at a wide range of depths and is a multiple spawner. The L50’s for females and males at first maturity, i.e. 301 and 320 mm, respectively, were attained by about the end of the third year of life and are well below the minimum legal length (MLL) of 500 mm. Because females and males did not reach the MLL until the end of their seventh and sixth years of life, respectively, they would have had, on average, the opportunity of spawning during four and three spawning seasons, respectively, before they reached the MLL. However, because G. hebraicum caught in water depths >40 m typically die upon release, a MLL is of limited use for conserving this species. Alternative approaches, such as restricting fishing activity in highly fished areas, reducing daily bag limits for recreational fishermen, introducing quotas or revising specific details of certain commercial hand-line licences (or doing both) are more likely to provide effective conservation measures.

The reproductive biology of the porbeage shark (Lamna nasus) in the western North Atlantic Ocean

Reproductive organs from 393 male and 382 female porbeagles (Lamna nasus), caught in the western North Atlantic Ocean, were examined to determine size at maturity and reproductive cycle. Males ranged in size from 86 to 246 cm fork length (FL) and females ranged from 94 to 288 cm FL. Maturity in males was best described by an inflection in the relationship of clasper length to fork length when combined with clasper calcification. Males matured between 162 and 185 cm FL and 50% were mature at 174 cm FL. In females, all reproductive organ measurements related to body length showed a strong inflection around the size of maturity. Females matured between 210 and 230 cm FL and 50% were mature at 218 cm FL. After a protracted fall mating period (September–November), females give birth to an average of 4.0 young in spring (April−June). As in other lamnids, young are nourished through oophagy. Evidence from this study indicated a one-year reproductive cycle and gestation period lasting 8–9 months.

盐角草（Salicornia europaea L.）体外再生体系及floral-dip转化系统的初步研究

盐角草（Salicornia europaea L.）属藜科盐角草属（Salicornia），是迄今已报道过的最为耐盐的真盐生植物之一。跟与其同属的海蓬子（Salicornia bigelovvi Torr.）相比, 它的分布更为广泛。它的种子含油丰富，因此具有发展为油料作物的潜力。此外，它也可以作为蔬菜和饲料。盐角草的组织培养工作为未来的转化研究和胁迫相关基因功能分析提供了有力的工具。本工作中，通过器官发生途径，建立了盐角草的体外再生体系。以完整的成熟种子为起始培养材料，在添TDZ 0.1 mg/L与NAA 1 mg/L的MS培养基上，暗培养三周后在下胚轴处形成愈伤组织，形成愈伤的平均频率为99％。愈伤组织在含TDZ 0.1 mg/L与NAA 1 mg/L的培养基上培养3－4周后分化出芽，分化频率约26.7%。采用2,4-D短时处理法结合添加NaCl，经过6－8周的培养获得了丛生芽，提高了再生频率。分化芽3周后转入含IBA 0.5 mg/L、KN 0.1 mg/L与0.05％活性炭的1/2 MS培养基，3周后生根形成完整植株。同时，本研究也进行了从直接不定芽途径建立盐角草再生体系的试验，但未获成功。 此外，本工作借鉴拟南芥的floral-dip转化法，对建立盐角草floral-dip转化系统进行了尝试。

Biology and feeding behavior of Orbinia johnsoni

Orbinia johnsoni were studied from a small sandy beach near Mussel Pt., Pacific Grove, California, where they are most abundant at low tide levels in fine sand. They were found to have a mean length of 190 mm. The orbiniids were found with their guts the fullest during incoming to high tides. It is plausible that this is when they are feeding. It takes about 3 to 3.5 hours for food to travel through the length fo the gut. The orbiniids eat 93 percent sand and seven percent organic detritus. Special note should be taken that some food selectivity appears to be involved and that high percentages of organic matter in the feces are found in worms collected during low, outgoing tides. Evidence suggests that the worms are bottom feeders, not coming to the surface to feed.

Report on the biology of Pacific hagfish, Eptatretus stouti and the development of its fishery in California

Hagfish, often referred to as "slime eels", are familiar to most fishermen as pests that frequently devour fish caught by trap, hook, and gillnet. In the Republic of Korea (ROK or South Korea), however, hagfish are sought after as valuable fish not only for their edible flesh, but also for their skin, which is processed into leather used to make expensive purses, shoes, and other articles. In fact, because of a shortage of hagfish in the waters near the ROK, the leather industry there has started to import hagfish, first from Japan in the mid 1980's, then from the United States starting in 1988. This report describes the nascent fishery for Eptatretus stouti (Pacific hagfish) in California, and includes aspects of its life history. The hagfish industry in the ROK is also briefly described.

Biology and morphometry study of Drepane punctata (Cuv and Val) at juvenile stage of Bombay coast

The metric and meristic characters of Drepane punctata from Versova Fish Landing Centre of Bombay have been studied. The meristic characters showed considerable variations. The number of dorsal fin rays were relatively stable characters. Statistical interpretation of metric data indicated that there is a direct relationship between the preanal, predorsal in relation to total length and eye-diameter in relation to head length.