Rapid and sensitive hormonal profiling of complex plant samples by liquid chromatography coupled to electrospray ionization tandem mass spectrometry

Background Plant hormones play a pivotal role in several physiological processes during a plant's life cycle, from germination to senescence, and the determination of endogenous concentrations of hormones is essential to elucidate the role of a particular hormone in any physiological process. Availability of a sensitive and rapid method to quantify multiple classes of hormones simultaneously will greatly facilitate the investigation of signaling networks in controlling specific developmental pathways and physiological responses. Due to the presence of hormones at very low concentrations in plant tissues (10-9 M to 10-6 M) and their different chemistries, the development of a high-throughput and comprehensive method for the determination of hormones is challenging. Results The present work reports a rapid, specific and sensitive method using ultrahigh-performance liquid chromatography coupled to electrospray ionization tandem spectrometry (UPLC/ESI-MS/MS) to analyze quantitatively the major hormones found in plant tissues within six minutes, including auxins, cytokinins, gibberellins, abscisic acid, 1-amino-cyclopropane-1-carboxyic acid (the ethylene precursor), jasmonic acid and salicylic acid. Sample preparation, extraction procedures and UPLC-MS/MS conditions were optimized for the determination of all plant hormones and are summarized in a schematic extraction diagram for the analysis of small amounts of plant material without time-consuming additional steps such as purification, sample drying or re-suspension. Conclusions This new method is applicable to the analysis of dynamic changes in endogenous concentrations of hormones to study plant developmental processes or plant responses to biotic and abiotic stresses in complex tissues. An example is shown in which a hormone profiling is obtained from leaves of plants exposed to salt stress in the aromatic plant, Rosmarinus officinalis.

Large-scale association analyses identify new loci influencing glycemic traits and provide insight into the underlying biological pathways.

Through genome-wide association meta-analyses of up to 133,010 individuals of European ancestry without diabetes, including individuals newly genotyped using the Metabochip, we have increased the number of confirmed loci influencing glycemic traits to 53, of which 33 also increase type 2 diabetes risk (q < 0.05). Loci influencing fasting insulin concentration showed association with lipid levels and fat distribution, suggesting impact on insulin resistance. Gene-based analyses identified further biologically plausible loci, suggesting that additional loci beyond those reaching genome-wide significance are likely to represent real associations. This conclusion is supported by an excess of directionally consistent and nominally significant signals between discovery and follow-up studies. Functional analysis of these newly discovered loci will further improve our understanding of glycemic control.

Genome-wide association study identifies loci influencing concentrations of liver enzymes in plasma.

Concentrations of liver enzymes in plasma are widely used as indicators of liver disease. We carried out a genome-wide association study in 61,089 individuals, identifying 42 loci associated with concentrations of liver enzymes in plasma, of which 32 are new associations (P = 10(-8) to P = 10(-190)). We used functional genomic approaches including metabonomic profiling and gene expression analyses to identify probable candidate genes at these regions. We identified 69 candidate genes, including genes involved in biliary transport (ATP8B1 and ABCB11), glucose, carbohydrate and lipid metabolism (FADS1, FADS2, GCKR, JMJD1C, HNF1A, MLXIPL, PNPLA3, PPP1R3B, SLC2A2 and TRIB1), glycoprotein biosynthesis and cell surface glycobiology (ABO, ASGR1, FUT2, GPLD1 and ST3GAL4), inflammation and immunity (CD276, CDH6, GCKR, HNF1A, HPR, ITGA1, RORA and STAT4) and glutathione metabolism (GSTT1, GSTT2 and GGT), as well as several genes of uncertain or unknown function (including ABHD12, EFHD1, EFNA1, EPHA2, MICAL3 and ZNF827). Our results provide new insight into genetic mechanisms and pathways influencing markers of liver function.

Igneous layering, fractional crystallization and growth of granitic plutons: The Dolbel batholith in SW Niger

This study reassesses the development of compositional layering during the growth of granitic plutons, with emphasis on fractional crystallization and its interaction with both injection and inflation-related deformation. The Dolbel batholith (SW Niger) consists of 14, kilometre-sized plutons emplaced by pulsed magma inputs. Each pluton has a coarse-grained core and a peripheral layered series. Rocks consist of albite (An(<= 11)), K-feldspar (Or(96 99), Ab(1) (4)), quartz, edenite (X(Mg)=0337-0.55), augite (X(Mg)=0.65-0.72) and accessories (apatite, titanite and Fe-Ti-oxides). Whole-rock compositions are metaluminous, sodic (K(2)O/Na(2)O=0.49-0.62) and iron-rich [FeO(tot)/(FeO(tot)+MgO)=0.65-0.82]. The layering is present as size-graded and modally graded, sub-vertical, rhythmic units. Each unit is composed of three layers, which are, towards the interior: edenite +/- plagioclase (C(a/p)), edenite+plagioclase+augite+quartz (C(q)), and edenite+plagioclase+augite+quartz+K-feldspar (C(k)). All phases except quartz show zoned microstructures consisting of external intercumulus overgrowths, a central section showing oscillatory zoning and, in the case of amphibole and titanite, complexly zoned cores. Ba and Sr contents of feldspars decrease towards the rims. Plagioclase crystal size distributions are similar in all units, suggesting that each unit experienced a similar thermal history. Edenite, characteristic of the basal C(a/p) layer, is the earliest phase to crystallize. Microtextures and phase diagrams suggest that edenite cores may have been brought up with magma batches at the site of emplacement and mechanically segregated along the crystallized wall, whereas outer zones of the same crystals formed in situ. The subsequent C(q) layers correspond to cotectic compositions in the Qz-Ab-Or phase diagram at P(H2O)=5 kbar. Each rhythmic unit may therefore correspond to a magma batch and their repetition to crystallization of recurrent magma recharges. Microtextures and chemical variations in major phases allow four main crystallization stages to be distinguished: (1) open-system crystallization in a stirred magma during magma emplacement, involving dissolution and overgrowth (core of edenite and titanite crystals); (2) in situ fractional crystallization in boundary layers (C(a/p) and C(q) layers); (3) equilibrium `en masse' eutectic crystallization (C(k) layers); (4) compaction and crystallization of the interstitial liquid in a highly crystallized mush (e. g. feldspar intercumulus overgrowths). It is concluded that the formation of the layered series in the Dolbel plutons corresponds principally to in situ differentiation of successive magma batches. The variable thickness of the Ck layers and the microtextures show that crystallization of a rhythmic unit stops and it is compacted when a new magma batch is injected into the chamber. Therefore, assembly of pulsed magma injections and fractional crystallization are independent, but complementary, processes during pluton construction.

Radarsat backscattering from an agricultural scene

Orbital remote sensing in the microwave electromagnetic region has been presented as an important tool for agriculture monitoring. The satellite systems in operation have almost all-weather capability and high spatial resolution, which are features appropriated for agriculture. However, for full exploration of these data, an understanding of the relationships between the characteristics of each system and agricultural targets is necessary. This paper describes the behavior of backscattering coefficient (sigma°) derived from calibrated data of Radarsat images from an agricultural area. It is shown that in a dispersion diagram of sigma° there are three main regions in which most of the fields can be classified. The first one is characterized by low backscattering values, with pastures and bare soils; the second one has intermediate backscattering coefficients and comprises well grown crops mainly; and a third one, with high backscattering coefficients, in which there are fields with strong structures causing a kind of double bounce effect. The results of this research indicate that the use of Radarsat images is optimized when a multitemporal analysis is done making the best use of the agricultural calendar and of the dynamics of different cultures.

Nullification of small knots

in the paper we consider the nullification number of small knots with at most 9 crossings. We establish two inequalities (Corollary 2.1) relating the nullification number to other knot invariants and properties of the knot diagram. We show that these inequalities allow us to settle the nullification number for all of the 84 prime knots with at most 9 crossings.

Biased diffusion in confined media: Test of the Fick-Jacobs approximation and validity criteria

We study biased, diffusive transport of Brownian particles through narrow, spatially periodic structures in which the motion is constrained in lateral directions. The problem is analyzed under the perspective of the Fick-Jacobs equation, which accounts for the effect of the lateral confinement by introducing an entropic barrier in a one-dimensional diffusion. The validity of this approximation, based on the assumption of an instantaneous equilibration of the particle distribution in the cross section of the structure, is analyzed by comparing the different time scales that characterize the problem. A validity criterion is established in terms of the shape of the structure and of the applied force. It is analytically corroborated and verified by numerical simulations that the critical value of the force up to which this description holds true scales as the square of the periodicity of the structure. The criterion can be visualized by means of a diagram representing the regions where the Fick-Jacobs description becomes inaccurate in terms of the scaled force versus the periodicity of the structure.

A partial ordering of knots and links through diagrammatic unknotting

In this paper we de. ne a partial ordering of knots and links using a special property derived from their minimal diagrams. A link K' is called a predecessor of a link K if Cr(K') < Cr(K) and a diagram of K' can be obtained from a minimal diagram D of K by a single crossing change. In such a case, we say that K' < K. We investigate the sets of links that can be obtained by single crossing changes over all minimal diagrams of a given link. We show that these sets are specific for different links and permit partial ordering of all links. Some interesting results are presented and many questions are raised.

Decision-theoretic reflections on processing a fingermark

A recent publication in this journal [Neumann et al., Forensic Sci. Int. 212 (2011) 32-46] presented the results of a field study that revealed the data provided by the fingermarks not processed in a forensic science laboratory. In their study, the authors were interested in the usefulness of this additional data in order to determine whether such fingermarks would have been worth submitting to the fingermark processing workflow. Taking these ideas as a starting point, this communication here places the fingermark in its context of a case brought before a court, and examines the question of processing or not processing a fingermark from a decision-theoretic point of view. The decision-theoretic framework presented provides an answer to this question in the form of a quantified expression of the expected value of information (EVOI) associated with the processed fingermark, which can then be compared with the cost of processing the mark.

Reaction-diffusion lattice gas: Theory and computer results

We report on the study of nonequilibrium ordering in the reaction-diffusion lattice gas. It is a kinetic model that relaxes towards steady states under the simultaneous competition of a thermally activated creation-annihilation $(reaction$) process at temperature T, and a diffusion process driven by a heat bath at temperature T?T. The phase diagram as one varies T and T, the system dimension d, the relative priori probabilities for the two processes, and their dynamical rates is investigated. We compare mean-field theory, new Monte Carlo data, and known exact results for some limiting cases. In particular, no evidence of Landau critical behavior is found numerically when d=2 for Metropolis rates but Onsager critical points and a variety of first-order phase transitions.

Paisatge, poblament, cultura material i història de l'Ager Tarraconensis. Resultats i comparacions

Síntesi dels primers resultats del Projecte Ager Tarraconensis i de les aportacions al simposi, de les quals s’extreuen reflexions i coneixements nous sobre el tema de l’estudi entorn del conjunt de l’espai de la ciutat romana, del paisatge, tractat com un sistema. Les aportacions se centren en la varietat de les primeres estratègies de control romà del territori, de les tipologies i evolució dels establiments, dels aspectes econòmics i de la dinàmica del poblament. Tot plegat fa referència molt especialment al territori de Tarraco, per bé que es fan nombroses comparacions amb altres territoris de l’àrea catalana i saguntina i també d’altres províncies romanes. Tarraco es va perfilant com una ciutat amb un territorium molt productiu, no sols en agricultura, sinó també en indústries, producció tèxtil, explotacions de recursos minerals com les pedreres i el ferro, i amb un comerç molt actiu que la devia convertir en un port important de la Mediterrània occidental romana. La jerarquia dels hàbitats rurals també s’està demostrant més complexa gràcies al coneixement de noves aglomeracions. L’evolució posterior al període romà també ha entrat en discussió, especialment pel que fa a l’època islàmica, sobre la qual es discuteix si el camp va quedar poblat o despoblat, tema que resol en sentit positiu el diagrama pol·línic realitzat dins del marc del projecte.

Ohutlevytuotteenvalmistusteknisten piirteiden tunnistaminen ja käyttö suunnittelun apuna

Suunnittelu- ja valmistustoiminnot ovat eriytymässä myös ohutlevyteollisuudessa. Kilpailukyvyn parantamiseksi, valmistusta siirretään alihankkijoille maihin, jossa työvoima on halpaa ja suuret markkinat lähellä, tuotekehityksen ja suunnittelun jäädessä Suomeen tai muualle Länsi-Eurooppaan. Tällöin vanhan lokaalin toimintamallin synergiaedut eivät ole enää mahdollisia ja tuotteen valmistettavuuden arviointi, laadunhallinta ja komponenttien yhteensopivuuden varmistaminen on erittäin haasteellista. Tässä tutkimuksessa etsittiin uuden toimintamallin tuomiin haasteisiin vastauksia piirrepohjaisesta mallintamisesta. Tavoitteen mukaisesti, tutkimuksessa tunnistettiin ohutlevytuotteen valmistusteknilliset piirteet ja luotiin niiden mukainen piirrekaavio. Piirteiden tunnistus vaati tutkimustyötä sekä nykyaikaisten suunnittelumetodien että valmistusmenetelmien ja ohutlevymateriaalien parissa. Lisäksitarkasteltiin, millaisia vaikutuksia piirteillä on tuotteen valmistettavuuteen ja miten nämä tulee huomioida tuotteen suunnittelussa.

Lämmöntalteenotto kosteasta poistoilmasta prosessiteollisuudessa

Tämän diplomityön päämääränä on tehdä prosessiteollisuuden tarpeisiin Excel-taulukkolaskentaohjelmassa toimiva putkilämmönsiirtimen mitoitusohjelma. Prosessiteollisuudessa lämmönvaihtimien toimintaympäristöt ja olosuhteet vaihtelevat merkittävästi ja niinpä jokaisen vaihtimen suunnittelu ja mitoitus on toteutettava tapauskohtaisesti. Työssä käsitellään rekuperatiivisen ristivirtaputkilämmönvaihtimen yleinen lämpötekninen mitoitus sisältäen putken sisäpinnalle tapahtuvan mahdollisen lauhtumisen. Mitoitettava vaihdinkoostuu pystysuorista putkista, joissa lämmin ja kostea ilma virtaa putkien sisäpuolella ja kylmä kuiva ilma vaippapuolella vaakasuoraan. Vaihdinmateriaalina käytetään ruostumatonta AISI 304 -tai haponkestävää AISI 316 terästä. Kuuman ilman tila vaihtelee tarkasteltavan kohteen mukaan. Paperiteollisuuden kuivausyksiköiltä poistuva ilma on usein lämmintä ja kosteaa, ja infrakuivaimilta poistuva ilma on kuumaa. Mitoitettavalle lämmönvaihtimelle tulevan kuuman ilman lämpötila tapauksesta riippuen voi vaihdella 30°C, maksimissaan +300°C:een saakka, vesisisällön ollessa välillä 0,010...0,200 kg/kg ki tai jopa tämän ylikin. Vaihtimen mitoitus perustuu energiataseyhtälöiden käyttöön. Laskennassa määritetään vaihtimen pintalämpötila sekä mahdollinen kostean ilman lauhtuminen putken sisäpinnalle. Lisäksi teoria käsittää molempien virtausten tilanmuutosten laskennan. Työssä on esitetty esimerkkilaskelma, jossa on laskettu ilma- kostea ilma lämmönsiirrinkonstruktio. Esimerkissä on tarkasteltu vaihtimen hyötysuhdetta, virtausten lämpö- ja kosteuskäyttäytymistä ulkoilman lämpötilan funktiona. Ohjelmasta saadaan tulostettua mitoitettavanvaihtimen dimensiot; putkien lukumäärät syvyys- ja pituussuunnassa sekä kokonaisputkilukumäärä, putkien väliset etäisyydet toisiinsa nähden sekä syvyys, että pituussuunnassa, putkipituus ja putken sisä- ja ulkohalkaisijat. Nämä tiedot suunnittelija itse syöttää ohjelmalle alkuarvoina. Laskettuna tietona ohjelma antaa molempien virtausten poistolämpötilat, kuuman ilman poistuvan absoluuttisen kosteuden, kondenssivesimäärän, vaihtimen tehon ja painehäviöt vaippa- ja putkipuolelle. Lisäksi ohjelma laskee kuuman ilman ominaisentalpiat vaihtimen sisään- ja ulostulossa. Tämä mahdollistaa ilman tilapisteiden piirtämisen Mollier-piirrokseen.

The Lin28/let-7 axis regulates glucose metabolism.

The let-7 tumor suppressor microRNAs are known for their regulation of oncogenes, while the RNA-binding proteins Lin28a/b promote malignancy by inhibiting let-7 biogenesis. We have uncovered unexpected roles for the Lin28/let-7 pathway in regulating metabolism. When overexpressed in mice, both Lin28a and LIN28B promote an insulin-sensitized state that resists high-fat-diet induced diabetes. Conversely, muscle-specific loss of Lin28a or overexpression of let-7 results in insulin resistance and impaired glucose tolerance. These phenomena occur, in part, through the let-7-mediated repression of multiple components of the insulin-PI3K-mTOR pathway, including IGF1R, INSR, and IRS2. In addition, the mTOR inhibitor, rapamycin, abrogates Lin28a-mediated insulin sensitivity and enhanced glucose uptake. Moreover, let-7 targets are enriched for genes containing SNPs associated with type 2 diabetes and control of fasting glucose in human genome-wide association studies. These data establish the Lin28/let-7 pathway as a central regulator of mammalian glucose metabolism.

AV37 - Diagrama funcional de un producto del ámbito industrial: el microondas

Aplicación práctica del método de análisis funcional de productos industriales. Análisis funcional para un microondas doméstico y alternativas de diseño derivadas