Leopold Letter: A Newsletter of the Leopold Center for Sustainable Agriculture, Winter 2006

The Leopold Center was created by the Iowa Legislature as part of the Iowa Groundwater Protection Act of 1987. The Leopold Center believes contribute to a healthy ways of thinking about markets for Iowa farmers, a better understanding of local ecosystems, public policies and economic practices, and partnerships with consumers.

N-Glycosylation and conserved cysteine residues in RAMP3 play a critical role for the functional expression of CRLR/RAMP3 adrenomedullin receptor.

The calcitonin receptor-like receptor (CRLR) and receptor activity modifying protein-3 (RAMP3) can assemble into a CRLR/RAMP3 heterodimeric receptor that exhibits the characteristics of a high affinity adrenomedullin receptor. RAMP3 participates in adrenomedullin (AM) binding via its extracellular N-terminus characterized by the presence of six highly conserved cysteine residues and four N-glycosylation consensus sites. Here, we assessed the usage of these conserved residues in cotranslational modifications of RAMP3 and addressed their role in functional expression of the CRLR/RAMP3 receptor. Using a Xenopus oocyte expression system, we show that (i) RAMP3 is assembled with CRLR as a multiple N-glycosylated species in which two, three, or four consensus sites are used; (ii) elimination of all N-glycans in RAMP3 results in a significant inhibition of receptor [(125)I]AM binding and an increase in the EC(50) value for AM; (iii) several lines of indirect evidence indicate that each of the six cysteines is involved in disulfide bond formation; (iv) when all cysteines are mutated to serines, RAMP3 is N-glycosylated at all four consensus sites, suggesting that disulfide bond formation inhibits N-gylcosylation; and (v) elimination of all cysteines abolishes adrenomedullin binding and leads to a complete loss of receptor function. Our data demonstrate that cotranslational modifications of RAMP3 play a critical role in the function of the CRLR/RAMP3 adrenomedullin receptor.

Leopold Center for Sustainable Agriculture Annual Report, 1998-1999

The Leopold Center was established by the Iowa Legislature as part of the Iowa Groundwater Protection Act of 1987. Its mandated missions are to identify impacts of agricultural practices, contribute to the development of profitable farming systems that conserve natural resources, and cooperate with Iowa State University Extension to inform the public of new findings.Information for this report was compiled by Leopold Center staff with the help of its researchers and educators who are committed to improving Iowa agriculture and the lives of Iowans.

Leopold Center for Sustainable Agriculture Annual Report, 1999-2000

The Leopold Center was established by the Iowa Legislature as part of the Iowa Groundwater Protection Act of 1987. Its mandated missions are to identify impacts of agricultural practices, contribute to the development of profitable farming systems that conserve natural resources, and cooperate with Iowa State University Extension to inform the public of new findings.Information for this report was compiled by Leopold Center staff with the help of its researchers and educators who are committed to improving Iowa agriculture and the lives of Iowans.

Leopold Center for Sustainable Agriculture Annual Report, 2000-2001

The Leopold Center was established by the Iowa Legislature as part of the Iowa Groundwater Protection Act of 1987. Its mandated missions are to identify impacts of agricultural practices, contribute to the development of profitable farming systems that conserve natural resources, and cooperate with Iowa State University Extension to inform the public of new findings.Information for this report was compiled by Leopold Center staff with the help of its researchers and educators who are committed to improving Iowa agriculture and the lives of Iowans.

Leopold Center for Sustainable Agriculture Annual Report, 2001-2002

The Leopold Center was established by the Iowa Legislature as part of the Iowa Groundwater Protection Act of 1987. Its mandated missions are to identify impacts of agricultural practices, contribute to the development of profitable farming systems that conserve natural resources, and cooperate with Iowa State University Extension to inform the public of new findings.Information for this report was compiled by Leopold Center staff with the help of its researchers and educators who are committed to improving Iowa agriculture and the lives of Iowans.

Leopold Center for Sustainable Agriculture Annual Report, 2002-2003

The Leopold Center was established by the Iowa Legislature as part of the Iowa Groundwater Protection Act of 1987. Its mandated missions are to identify impacts of agricultural practices, contribute to the development of profitable farming systems that conserve natural resources, and cooperate with Iowa State University Extension to inform the public of new findings.Information for this report was compiled by Leopold Center staff with the help of its researchers and educators who are committed to improving Iowa agriculture and the lives of Iowans.

Leopold Center for Sustainable Agriculture Annual Report, 2003-2004

The Leopold Center was established by the Iowa Legislature as part of the Iowa Groundwater Protection Act of 1987. Its mandated missions are to identify impacts of agricultural practices, contribute to the development of profitable farming systems that conserve natural resources, and cooperate with Iowa State University Extension to inform the public of new findings.Information for this report was compiled by Leopold Center staff with the help of its researchers and educators who are committed to improving Iowa agriculture and the lives of Iowans.

Leopold Center for Sustainable Agriculture Annual Report, 2004-2005

The Leopold Center was established by the Iowa Legislature as part of the Iowa Groundwater Protection Act of 1987. Its mandated missions are to identify impacts of agricultural practices, contribute to the development of profitable farming systems that conserve natural resources, and cooperate with Iowa State University Extension to inform the public of new findings.Information for this report was compiled by Leopold Center staff with the help of its researchers and educators who are committed to improving Iowa agriculture and the lives of Iowans.

Leopold Center for Sustainable Agriculture Annual Report, 2005-2006

The Leopold Center was established by the Iowa Legislature as part of the Iowa Groundwater Protection Act of 1987. Its mandated missions are to identify impacts of agricultural practices, contribute to the development of profitable farming systems that conserve natural resources, and cooperate with Iowa State University Extension to inform the public of new findings.Information for this report was compiled by Leopold Center staff with the help of its researchers and educators who are committed to improving Iowa agriculture and the lives of Iowans.

Leopold Center for Sustainable Agriculture Annual Report, 2006-2007

The Leopold Center was established by the Iowa Legislature as part of the Iowa Groundwater Protection Act of 1987. Its mandated missions are to identify impacts of agricultural practices, contribute to the development of profitable farming systems that conserve natural resources, and cooperate with Iowa State University Extension to inform the public of new findings.Information for this report was compiled by Leopold Center staff with the help of its researchers and educators who are committed to improving Iowa agriculture and the lives of Iowans.

Leopold Center for Sustainable Agriculture Annual Report, 2007-2008

The Leopold Center was established by the Iowa Legislature as part of the Iowa Groundwater Protection Act of 1987. Its mandated missions are to identify impacts of agricultural practices, contribute to the development of profitable farming systems that conserve natural resources, and cooperate with Iowa State University Extension to inform the public of new findings.Information for this report was compiled by Leopold Center staff with the help of its researchers and educators who are committed to improving Iowa agriculture and the lives of Iowans.

Leopold Center for Sustainable Agriculture Annual Report, 2008-2009

The Leopold Center was established by the Iowa Legislature as part of the Iowa Groundwater Protection Act of 1987. Its mandated missions are to identify impacts of agricultural practices, contribute to the development of profitable farming systems that conserve natural resources, and cooperate with Iowa State University Extension to inform the public of new findings.Information for this report was compiled by Leopold Center staff with the help of its researchers and educators who are committed to improving Iowa agriculture and the lives of Iowans.

Leopold Center for Sustainable Agriculture Annual Report, 2009-2010

The Leopold Center was established by the Iowa Legislature as part of the Iowa Groundwater Protection Act of 1987. Its mandated missions are to identify impacts of agricultural practices, contribute to the development of profitable farming systems that conserve natural resources, and cooperate with Iowa State University Extension to inform the public of new findings.Information for this report was compiled by Leopold Center staff with the help of its researchers and educators who are committed to improving Iowa agriculture and the lives of Iowans.

Leopold Center for Sustainable Agriculture Annual Report, 2010-2011

The Leopold Center was established by the Iowa Legislature as part of the Iowa Groundwater Protection Act of 1987. Its mandated missions are to identify impacts of agricultural practices, contribute to the development of profitable farming systems that conserve natural resources, and cooperate with Iowa State University Extension to inform the public of new findings.Information for this report was compiled by Leopold Center staff with the help of its researchers and educators who are committed to improving Iowa agriculture and the lives of Iowans.