Untersuchung von chlorierten Kohlenwasserstoffen (CKW) an Flunder (Platichtbys flesus) und Scholle (Pleuronectes platessa) aus der Nordsee

Im Rahmen des Internationalen Rates für Meeresforschung werden seit 1972 Studien zur Kontamination von Meerestieren der Nordsee mit Schadstoffen erhoben. Eine Intensivierung dieses Unternehmens erfolgte durch die Schaffung eines gemeinsamen internationalen Überwachungsprogrammes (Joint Monitoring Program) durch die Kommission von Oslo und Paris. In diesem Programm sind die Überwchungsgebiete für die Bundesrepublik vorgeschrieben. Auch Species, Anzahl und Gewebe sind festgelegt.

Some observations on the temporal variations of size of copepods and some answers to questions on their biology. [Translation from: Internationale Revue der Gesamten Hydrobiologie und Hydrographie 17 99-114, 1927.]

Until now observations on the temporal variation of size of freshwater copepods have not provided much information. Other observers only mention in passing this or that phenomenon from which it is possible to deduct termporal variations. In this study Cyclops strenuus s.l., a freshwater species of fairly wide distribution, is studied in two water bodies. The author studies the systematic, placing of inhabitants described as C. strenuus Fischer in both locations, their annual life cycle, and their annual size variations.

Investigations on the vertical migration of planktonic Crustacea in the Bodensee-Obersee. [Translation from: Schriften des Vereins fur Geschichte des Bodensees und seiner Umgebung, 87, 177-187, 1969.]

The vertical zoning of the planktonic Crustacea in a lake is the expression of a complex set of different factors. Besides the measurable, external influences such as light, temperature, acid and C02 stratification, a particularly large part is played by internal factors, which co-ordinate a specific reaction in each species depending on state of development, age and sex. Supporting this extensive, predictable, annual course of diurnal depths and the daily vertical migrations, whose extent is again dependent on external conditions, primarily of course on the amount of light. The individual factors mentioned, however, are here also of great significance. Within the scope of a long-term study of the planktonic Copepoda of Lake Constance, some day and night series were in 1963 also carried out in the Obersee, in order to obtain at least volumetric data on the extent of the daily migrations of these creatures.

A study of flow conditions near the substratum in an experimental channel [Translation from: Verhandlungen der Internationalen Vereinigung fur Theoretische und Angewandte Limnologie 18, 718-725, 1972]

In a slow flow, on a smooth uniform substratum, a limited bed allows the existence of currents slow enough for benthic invertebrates. These conditions rarely occur naturally. The investigations carried out in this work aimed, on an intermediary scale, to define the influence of irregularities in the substratum on flow near the bottom. The substrata used were made of glass marbles. The investigations were carried out in a transparent channel of 70 cm in length and a rectangular section 10 x 5 cm. The data was analysed to study the general evolution of flow in terms of average speeds and the appearance of the turbulence near the bottom.

Fish zoning of some watercourses in Normandy [Translation from: Verhandlungen der Internationalen Vereinigung fuer Theoretische und Angewandte Limnologie 18, 1135-1146, 1972]

The identification of fish zones in western and central Europe has been the objective some major studies. This paper concerns a stream in Normandy specially studied by the author in 1969 and 1970 in the framework of a study on the role of the mean temperature in fish zoning. The paper propounds the comparative study of the morphodynamic and occasionally physico-chemical characteristics, as well as the results of previous sampling by electric fishing of the fish populations of two other streams of the higher Cretaceous layer and supplied by ”chalky” water in Normandy and Picardy.

Differenzierung von Pangasius (Pangasius hypophthalmus) und Asiatischem Rotflossenwels (Hemibagrus wyckioides) durch Protein- und DNA-Analyse

In the last years farmed Pangasius (Tra-Pangasius, Pangasius hypophthalmus) from Vietnam has reached a considerable market share, whereas aquaculture of Asian Redtail Catfish (Hemibagrus wyckioides) is in its infancy. Recently it has been detected by food control authorities in Hamburg, that Pangasius fillets have been mislabelled and sold as fillets produced from Asian Redtail catfish. The necessity to improve the analytical methods for differentiation of Pangasius and Redtail Catfish prompted us to evaluate the suitability of isoelectric focusing (IEF) and DNA-analysis for identification of the two species. IEF of water soluble proteins was found to be a fast, reliable and economical method for differentiation of raw fillets of Pangasius and Redtail Catfish, as long as reference material is available. PCR-based DNA analysis was performed as follows: (i) amplification of a 464 bp segment of the cytochrome b gene; (ii) sequencing of the PCR product; (iii) comparison of the sequence with entries in GenBank using BLAST. The sequences of both species differed considerably, allowing the unequivocal differentiation between P. hypophthalmus and H. wyckioides. Kurzfassung Pangasius (Schlankwels, Tra-Pangasius, Pangasius hypophthalmus) hat sich innerhalb weniger Jahre zu einem bedeutenden Zuchtfisch entwickelt, während die Aquakultur des Asiatischen Rotflossenwelses (Hemibagrus wyckioides) in Vietnam noch in einem relativ kleinen Maßstab stattfindet. Kürzlich wurde von der Lebensmittelüberwachung in Hamburg nachgewiesen, dass im Handel erhältliche Filets mit der Deklaration „Rotflossenwels“ aus Pangasius hergestellt worden waren. Vor diesem Hintergrund wurden zwei Methoden auf ihre Eignung zur Differenzierung von Pangasius und Rotflossenwels geprüft. Es zeigte sich, dass sowohl die isoelektrische Fokussierung (IEF) wasserlöslicher Proteine als auch die PCR-basierte DNA-Analyse zur Unterscheidung beider Arten gut geeignet ist. Die IEF stellt eine schnelle und kostengünstige Untersuchungsmethode dar, die allerdings Referenzmaterial benötigt. Mit Hilfe der PCR (Polymerase-Kettenreaktion) wurde ein Abschnitt des Cytochrom b-Gens vervielfältigt und sequenziert. Die Sequenzen von P. hypophthalmus und H. wyckioides wiesen beträchtliche Unterschiede auf. Es wird diskutiert, wie sich durch Vergleich dieser Sequenzen mit Einträgen in Gendatenbanken unbekannte Proben beider Arten sicher zuordnen lassen.

Der Befall von Seelachsfilets mit Nematodenlarven und deren Abtötung durch Tiefgefrieren

Im Rahmen unserer Untersuchungen zum Befall von Seefischen mit Nematodenlarven und deren Abtötung unter industriellen Verarbeitungsbedingungen wurden während der 88. Reise des FFS "Walther Herwig" vom 23.1.88-17.2.88 u.a. Seelachs (Köhler; Pollachius virens) verschiedener Größenklassen untersucht.