Agreed-upon procedures report on the City of Griswold, Iowa for the period July 1, 2013 through June 30, 2014

Agreed-upon procedures report on the City of Griswold, Iowa for the period July 1, 2013 through June 30, 2014

Evaluation of the compliance with recommended procedures in newborns exposed to HBsAg-positive mothers: a multicenter collaborative study.

BACKGROUND: Maternal-infant transmission of hepatitis B virus (HBV) during birth carries a high risk for chronic HBV infection in infants with frequent subsequent development of chronic disease. This can be efficiently prevented by early immunization of exposed newborns. The purpose of this study was to determine the compliance with official recommendations for prevention of perinatal HBV transmission in hepatitis B surface antigen (HBsAg) exposed infants. METHODS: Records of pregnant women at 4 sites in Switzerland, admitted for delivery in 2005 and 2006, were screened for maternal HBsAg testing. In HBsAg-exposed infants, recommended procedures (postnatal active and passive immunization, completion of immunization series, and serological success control) were checked. RESULTS: Of 27,131 women tested for HBsAg, 194 (0.73%) were positive with 196 exposed neonates. Of these neonates, 143 (73%) were enrolled and 141 (99%) received simultaneous active and passive HBV immunization within 24 hours of birth. After discharge, the HBV immunization series was completed in 83%. Only 38% of children were tested for anti-HBs afterwards and protective antibody values (>100 U/L) were documented in 27% of the study cohort. No chronically infected child was identified. Analysis of hospital discharge letters revealed significant quality problems. CONCLUSIONS: Intensified efforts are needed to improve the currently suboptimal medical care in HBsAg-exposed infants. We propose standardized discharge letters, as well as reminders to primary care physicians with precise instructions on the need to complete the immunization series in HBsAg-exposed infants and to evaluate success by determination of anti-HBs antibodies after the last dose.

Membrane interaction of polymyxin B and synthetic analogues studied in biomimetic systems: implications for antibacterial action

Membrane-active antimicrobial peptides, such as polymyxin B (PxB), are currently in the spotlight as potential candidates toovercome bacterial resistance. We have designed synthetic analogs ofPxB in order to determine the structural requirements for membraneaction. Since the mechanism of action of PxB involves interaction withboth the outer membrane and the cytoplasmic membrane of Gramnegative bacteria, we have used an approach based on mimicking theouter layers of these membranes using monolayers, Langmuir-Blodgettfilms and unilamelar vesicles, and applying a battery of biophysicalmethods in order to dissect the different events of membraneinteraction. Collectively, results indicate that the PxB analogues act inthe bacterial membrane by the same mechanism than PxB, and that cationic amphipathicity determines peptide activity.

Agreed-upon procedures report on the City of Prairie City, Iowa for the period July 1, 2013 through June 30, 2014

Agreed-upon procedures report on the City of Prairie City, Iowa for the period July 1, 2013 through June 30, 2014

Agreed-upon procedures report on the City of Granger, Iowa for the period July 1, 2013 through June 30, 2014

Agreed-upon procedures report on the City of Granger, Iowa for the period July 1, 2013 through June 30, 2014

Agreed-upon procedures report on the City of Wall Lake, Iowa for the period July 1, 2013 through June 30, 2014

Agreed-upon procedures report on the City of Wall Lake, Iowa for the period July 1, 2013 through June 30, 2014

Agreed-upon procedures report on the City of New Vienna, Iowa for the period August 1, 2013 through July 31, 2014

Agreed-upon procedures report on the City of New Vienna, Iowa for the period August 1, 2013 through July 31, 2014

Agreed-upon procedures report on the City of Sidney, Iowa for the period July 1, 2013 through June 30, 2014

Agreed-upon procedures report on the City of Sidney, Iowa for the period July 1, 2013 through June 30, 2014

Agreed-upon procedures report on the City of Monroe, Iowa for the period July 1, 2013 through June 30, 2014

Agreed-upon procedures report on the City of Monroe, Iowa for the period July 1, 2013 through June 30, 2014

Agreed-upon procedures report on the City of Lambs Grove, Iowa for the period August 1, 2013 through July 31, 2014

Agreed-upon procedures report on the City of Lambs Grove, Iowa for the period August 1, 2013 through July 31, 2014

Agreed-upon procedures report on the City of Dallas Center, Iowa for the period July 1, 2013 through June 30, 2014

Agreed-upon procedures report on the City of Dallas Center, Iowa for the period July 1, 2013 through June 30, 2014

Protection against Plasmodium falciparum challenge induced in Aotus monkeys by liver-stage antigen-3-derived long synthetic peptides.

The vaccine potential of Plasmodium falciparum liver stage antigen-3 (LSA3) was investigated in Aotus monkeys using two long synthetic peptides corresponding respectively to an N-terminal non-repeat peptide (NRP) and repeat 2 (R2) region of the LSA3, adjuvanted by ASO2. Both 100-222 (NRP) and 501-596 repeat peptides induced effector B- and T-cell responses in terms of antigen-driven antibodies and/or specific IFN-gamma secretion. Animals challenged with P. falciparum sporozoites were protected following immunization with either the NRP region alone or the NRP combined with the R2 repeat region, as compared with controls receiving the adjuvant alone. These results indicate that the NRP may be sufficient to induce full, sterile protection and confirm the vaccine potential of LSA3 previously demonstrated in chimpanzees and in Aotus.

Agreed-upon procedures report on the City of Derby, Iowa for the period August 1, 2013 through July 31, 2014

Agreed-upon procedures report on the City of Derby, Iowa for the period August 1, 2013 through July 31, 2014

Agreed-upon procedures report on the City of Stanwood, Iowa for the period November 1, 2013 through October 31, 2014

Agreed-upon procedures report on the City of Stanwood, Iowa for the period November 1, 2013 through October 31, 2014

Agreed-upon procedures report on the City of Harper, Iowa for the period November 1, 2013 through October 31, 2014

Agreed-upon procedures report on the City of Harper, Iowa for the period November 1, 2013 through October 31, 2014