Real-time Biosensor for the Assessment of Nanotoxicity and Cancer Electrotherapy

Knowledge of cell electronics has led to their integration to medicine either by physically interfacing electronic devices with biological systems or by using electronics for both detection and characterization of biological materials. In this dissertation, an electrical impedance sensor (EIS) was used to measure the electrode surface impedance changes from cell samples of human and environmental toxicity of nanoscale materials in 2D and 3D cell culture models. The impedimetric response of human lung fibroblasts and rainbow trout gill epithelial cells when exposed to various nanomaterials was tested to determine their kinetic effects towards the cells and to demonstrate the biosensor’s ability to monitor nanotoxicity in real-time. Further, the EIS allowed rapid, real-time and multi-sample analysis creating a versatile, noninvasive tool that is able to provide quantitative information with respect to alteration in cellular function. We then extended the application of the unique capabilities of the EIS to do real-time analysis of cancer cell response to externally applied alternating electric fields at different intermediate frequencies and low-intensity. Decreases in the growth profiles of the ovarian and breast cancer cells were observed with the application of 200 and 100 kHz, respectively, indicating specific inhibitory effects on dividing cells in culture in contrast to the non-cancerous HUVECs and mammary epithelial cells. We then sought to enhance the effects of the electric field by altering the cancer cell’s electronegative membrane properties with HER2 antibody functionalized nanoparticles. An Annexin V/EthD-III assay and zeta potential were performed to determine the cell death mechanism indicating apoptosis and a decrease in zeta potential with the incorporation of the nanoparticles. With more negatively charged HER2-AuNPs attached to the cancer cell membrane, the decrease in membrane potential would thus leave the cells more vulnerable to the detrimental effects of the applied electric field due to the decrease in surface charge. Therefore, by altering the cell membrane potential, one could possibly control the fate of the cell. This whole cell-based biosensor will enhance our understanding of the responsiveness of cancer cells to electric field therapy and demonstrate potential therapeutic opportunities for electric field therapy in the treatment of cancer.

Towards a more sustainable aquaculture: diets effects and environmental impact of aquaculture from a microbiome perspective

There is a lot of interest to optimize aquaculture production due to its overexploitation of marine resources, ocean pollution and habitat destruction. Since feed production is one of the greatest issues in aquaculture, feeding strategy optimization is important. The study of several different feed additives or supplementation is important to secure optimal growth, gut health, and function in farmed fish. Feed additives are typically supplied to ensure good health and to help the animal ward off pathogens during both normal and challenging conditions, which could stress animals and promote insurgence of pathologies or pathogens invasions. In this context has an increasing interest the study of host associated microbiome to understand the influence of novel functional feed on the health and physiology of animals. To achieve a more sustainable aquaculture sector, show a great importance the understanding of the environmental impact of this human activity in terms of habitat destruction, ocean pollution and reduction marine environments biodiversity. Marine microbiomes, either free-living or associated with multicellular hosts, is acquiring an increasing interest because their role in supporting the functioning and biodiversity of marine ecosystems, providing essential ecological services. Becoming extremely important to understand how these activities can affect marine microbiomes by altering their function and diversity. In this thesis work, we were able to present a comprehensive evaluation of different functional feeds assessing their effects in terms of growth and gut health of three fish species, Rainbow Trout (Oncorhynchus mykiss), Gilthead seabream (Sparus aurata) and Zebrafish (Danio rerio). We also explored the impact of Aquaculture on the surrounding marine microbiomes, using Patella caerulea as a model holobionts. Finally, we provided a synoptical study on the microbiomes of the water column and surface sediments in North-Western Adriatic Sea (Italy), providing the finest-scale mapping of marine microbiomes in the Mediterranean Sea.

La pubblicità tra presenza LGBTQ+, stereotipi di genere ed inclusione. Le tendenze della rappresentazione queer all’interno degli spot televisivi americani

La pubblicità – quella televisiva passando poi a quella sul web e sui social – subisce numerose trasformazioni che investono soprattutto i metodi comunicativi con cui sviluppare la propria narrazione. Oggi, di fatto, il mondo della pubblicità non si limita alla sola sponsorizzazione di un brand e dei suoi prodotti, ma si cala sempre più nelle dinamiche sociali, civili ed economiche che caratterizzano il nostro quotidiano. La comunicazione commerciale, dunque, si prefigge di essere il più inclusiva possibile, di coinvolgere storie, situazioni e personaggi che, nella pubblicità del “passato”, sarebbero stati esclusi totalmente o rappresentati in modo stereotipato. Se risulta possibile affermare che, nel panorama mediale odierno, si stia rivolgendo maggiore attenzione alle tematiche LGBTQ+, alle lotte contro la discriminazione di genere e all’ingaggio di personaggi appartenenti alla comunità queer, è altrettanto vero affermare come sia ancora possibile ricadere in rappresentazioni stereotipate e strategie di marketing che si fingono inclusive, solo per cavalcare l’onda del successo di eventi quali il Pride. All’interno di questo elaborato si andrà, in primis, a tracciare la storia culturale della comunità LGBTQ+ e ad approfondire come sia evoluta la sua presenza nei diversi media audiovisivi – dal cinema alle serie tv fino alla pubblicità. Ci si concentrerà poi sugli stereotipi ancora ricorrenti in pubblicità televisiva, ossia quello di genere. L’obiettivo della tesi è verificare se sia giusto parlare di inclusione o se si tratti solo una mera strategia di marketing, ossia di rainbow-washing, quando i brand si avvalgono di immagini e di personaggi LGBTQ+ nei propri spot. Nel capitolo finale, attraverso di un’analisi qualitativa di n. 100 spot televisivi americani, si andranno ad evidenziare i trend LGBTQ+, ossia in che modo oggi le pubblicità statunitensi sviluppano immagini, simboli, personaggi e storie LGBTQ+, realmente positive ed inclusive, in pubblicità.