Richard Baxter Foster Letter to his wife September 14 1863

Letter from Richard Baxter Foster to his wife Lucy from St. Louis on September 14, 1863.

Daniel Foster Letter to Richard Baxter Foster May 11, 1863

Daniel Foster's letter to his brother Richard Baxter Foster from Camp of the 33rd Miss. Vol. near Aquin Creek, Virginia on May 11, 1863.

Richard Baxter Foster Letter to his wife March 7 1863

Letter from Richard Baxter Foster to his wife Lucy on March 7, 1863

Terapia génica dirigida en una nuevo "sitio seguro" en células progenitoras hematopoyéticas humanas para su aplicación en anemia de Fanconi

La anemia de Fanconi es una enfermedad hereditaria de baja prevalencia, descrita por primera vez por el pediatra Guido Fanconi en 1927. Esta enfermedad se produce como consecuencia de mutaciones en cualquiera de los 19 genes de Fanconi descritos hasta la actualidad, y que participan en la ruta de Fanconi/BRCA. Esta ruta se encarga de la reparación de enlaces intercatenarios del ADN y de coordinar los distintos mecanismos de reparación de las dobles roturas en el ADN. La anemia de Fanconi está caracterizada por generar inestabilidad genómica, lo que da lugar a anomalías esqueléticas y predisposición al cáncer, si bien la principal causa de muerte de pacientes pediátricos es el fallo de médula ósea. Uno de los tratamientos alternativos al trasplante alogénico de progenitores hematopoyéticos de pacientes con anemia de Fanconi se basa en la reinfusión de células madre hematopoyéticas autólogas, tras su corrección con vectores lentivirales. Para limitar al máximo los riesgos de este tipo de terapias se están desarrollando nuevas tecnologías de edición génica basadas en la inserción dirigida de los genes terapéuticos. Esta nueva aproximación se fundamenta en la generación de dobles roturas en regiones específicas del genoma, cuya reparación por recombinación homóloga facilitaría la entrada de los genes terapéuticos aportados por ADNs donadores externos con homología por dicha región. En este trabajo se ha desarrollado una aproximación de edición génica en un nuevo “sitio seguro” del genoma denominado SH6. Para ello se ha trabajado con la línea celular HEK-293H, así como también con progenitores hematopoyéticos humanos purificados en base a la expresión del marcador CD34. Para su desarrollo se han utilizado nucleasas de edición, tales como meganucleasas y TALEN, en combinación con matrices donadoras portadoras del gen marcador EGFP (GM) o del gen terapéutico FANCA (TM). En todos los casos los genes marcadores y terapéuticos estaban regulados por el promotor EF1α, y flanqueados por dos brazos de homología para el sitio SH6. Estos plásmidos han servido como molde para realizar la terapia génica de edición en el sitio seguro SH6...

Fortification and Frontier: The Project Drawn up by Juan Martín Zermeño for Puebla de Sanabria in 1766

Following the death of engineer General Jorge Próspero de Verboom in 1744 and after a few years of transition in the management of Spanish fortifications, Juan Martín Zermeño took on the role, initially with a temporary mandate, but then definitively during a second period that ran from 1766 until his death in 1772. He began this second period with a certain amount of concern because of what had taken place during the last period of conflict. The Seven Years War (1756–1763) which had brought Spain into conflict with Portugal and England in the Caribbean had also lead to conflict episodes along the Spanish–Portuguese border. Zermeño’s efforts as a planner and general engineer gave priority to the northern part of the Spanish–Portuguese border. After studying the territory and the existing fortifications on both sides of the border, Zermeño drew up three important projects in 1766. The outposts that needed to be reinforced were located, from north to south, at Puebla de Sanabria, Zamora and Ciudad Rodrigo, which is where he is believed to have come from. This latter township already had a modern installation built immediately after the war of the Spanish Succession and reinforced with the Fort of La Concepción. However, Zamora and Puebla de Sanabria had some obsolete fortifications that needed modernising. Since the middle of the 15th century Puebla de Sanabria had had a modern castle with rounded turrets, that of the counts of benavente. During the 16th and 17th centuries it had also been equipped with a walled enclosure with small bastions. During the war of the Spanish Succession the Portuguese had enlarged the enclosure and had erected a tentative offshoot to the west. In order to draw up the ambitious Puebla de Sanabria project Zermeño had the aid of some previous reports and projects, such as those by the count of robelin in 1722, the one by Antonio de Gaver in 1752, and Pedro Moreau’s report dated June 1755. This study includes a technical analysis of Zermeño’s project and its strategic position within the system of fortifications along the Spanish–Portuguese border.

England and Wales, 1863 (Raster Image)

This layer is a georeferenced raster image of the historic paper map entitled: Cruchley's travellers companion through England and Wales, shewing the railways and stations to the present time. It was published by G.F. Cruchley in 1863. Scale [ca. 1:950,400]. Covers also Isle of Man and shoreline of Northern Ireland.The image inside the map neatline is georeferenced to the surface of the earth and fit to the Europe Lambert Conformal Conic coordinate system. All map collar and inset information is also available as part of the raster image, including any inset maps, profiles, statistical tables, directories, text, illustrations, index maps, legends, or other information associated with the principal map. This map shows features such as drainage, cities and other human settlements, territorial boundaries, roads, railroads, ferry lines, telegraph lines, shoreline features, and more.This layer is part of a selection of digitally scanned and georeferenced historic maps from the Harvard Map Collection. These maps typically portray both natural and manmade features. The selection represents a range of originators, ground condition dates, scales, and map purposes.

Charleston Harbor, South Carolina, 1863 (Raster Image)

This layer is a georeferenced raster image of the historic paper map: Charleston Harbor and its approaches showing the positions of the Rebel-batteries, [by] U.S. Coast Survey. It was published in 1863 by Lith. of J. Bien. Scale 1:30,000. Nautical chart covering Charleston Harbor and a portion of Charleston, South Carolina. The image inside the map neatline is georeferenced to the surface of the earth and fit to the South Carolina State Plane Coordinate System (in Meters) (Fipszone 3900). All map collar and inset information is also available as part of the raster image, including any inset maps, profiles, statistical tables, directories, text, illustrations, or other information associated with the principal map. This map shows features such as roads, railroads, houses, vegetation, drainage, military batteries and fortifications, coastal features (shoals, rocks, channels, floating batteries, etc.) and more. Overprinted to show 1/4-mile concentric circles centered on St. Michaels, Charleston; positions occupied by the Union Army and Navy; "Rebel batteries in possession of National forces [and] batteries still held by the Rebels [on] Sept. 7th 1863." Union positions are based "on the authority of Maj. T.B. Brooks." Relief shown by hachures; depths shown by soundings and shading. This layer is part of a selection of digitally scanned and georeferenced historic maps of the Civil War from the Harvard Map Collection. Many items from this selection are from a collection of maps deposited by the Military Order of the Loyal Legion of the United States Commandery of the State of Massachusetts (MOLLUS) in the Harvard Map Collection in 1938. These maps typically portray both natural and manmade features, in particular showing places of military importance. The selection represents a range of regions, originators, ground condition dates, scales, and purposes.

Fort Wagner, Morris Island, South Carolina,1863 (Raster Image)

This layer is a georeferenced raster image of the historic paper manuscript map: Battery Wagner, Morris Isld., Francis D. Lee, Capt. Engrs. ; Langdon Cheves, Asst. Engr. in charge of work ; drawn by F.W. Bornemann, C.S. Engr. Office. It was drawn Nov 26, 1863. Scale [1:480]. The image inside the map neatline is georeferenced to the surface of the earth and fit to the South Carolina State Plane Coordinate System (in Meters) (Fipszone 3900). All map collar and inset information is also available as part of the raster image, including any inset maps, profiles, statistical tables, directories, text, illustrations, or other information associated with the principal map. This map shows features such as Fort dimensions and structures, landscape of area surrounding Fort, drainage, and more. This layer is part of a selection of digitally scanned and georeferenced historic maps of the Civil War from the Harvard Map Collection. Many items from this selection are from a collection of maps deposited by the Military Order of the Loyal Legion of the United States Commandery of the State of Massachusetts (MOLLUS) in the Harvard Map Collection in 1938. These maps typically portray both natural and manmade features, in particular showing places of military importance. The selection represents a range of regions, originators, ground condition dates, scales, and purposes.

Fort Sanders, Knoxville, Tennessee,and vicinity, 1863 (Raster Image)

This layer is a georeferenced raster image of the untitled historic paper manuscript map: [Map of Fort Sanders, Knoxville, Tennessee, and vicinity showing troop lines]. It was sketched ca.1863. Scale not given. The image inside the map neatline is georeferenced to the surface of the earth and fit to the Tennessee State Plane Coordinate System (in Meters) (Fipszone 4100). All map collar and inset information is also available as part of the raster image, including any inset maps, profiles, statistical tables, directories, text, illustrations, or other information associated with the principal map. This map shows features such as roads, railroads, drainage, troop location, fortifications, batteries, selected points of military interest, and more. Relief shown by hachures. This layer is part of a selection of digitally scanned and georeferenced historic maps of the Civil War from the Harvard Map Collection. Many items from this selection are from a collection of maps deposited by the Military Order of the Loyal Legion of the United States Commandery of the State of Massachusetts (MOLLUS) in the Harvard Map Collection in 1938. These maps typically portray both natural and manmade features, in particular showing places of military importance. The selection represents a range of regions, originators, ground condition dates, scales, and purposes.

New England Railroads, 1863 (Raster Image)

This layer is a georeferenced raster image of the historic paper map entitled: Map of railways in the New England states : engraved expressly for Snow's pathfinder railway guide, engraved in relief for Power Press Printing by W.C. Sharp. It was published in Snow's Pathfinder railway guide, week ending Saturday, May 9, 1863. Scale not given. Covers Vermont, New Hampshire, Massachusetts, Connecticut, Rhode Island, and portions of New York, Maine, and the provinces of Quebec and New Brunswick, Canada. The image inside the map neatline is georeferenced to the surface of the earth and fit to the USA Contiguous Albers Equal Area Conic projection (Meters). All map collar and inset information is also available as part of the raster image, including any inset maps, profiles, statistical tables, directories, text, illustrations, or other information associated with the principal map. This map shows features such as railroads completed and under construction, drainage, state and town boundaries, and more. Relief shown by hachures. Includes advertisements and index to selected towns in Mass. and N.H. and insets: Map of Boston and vicinity, 1860 -- New map of Boston, 1862. This layer is part of a selection of digitally scanned and georeferenced historic maps of New England from the Harvard Map Collection. These maps typically portray both natural and manmade features. The selection represents a range of regions, originators, ground condition dates, scales, and purposes.

Frankfurt am Main, Germany, 1863 (Raster Image)

This layer is a georeferenced raster image of the historic paper map entitled: Plan von Frankfurt [a], M. It was published ca. 1863. Scale [ca. 1:13,400]. Covers Frankfurt am Main, Germany. Map in German. The image inside the map neatline is georeferenced to the surface of the earth and fit to the 'Deutsches Hauptdreiecksnetz (DHDN) Gauss Kruger Zone 3, Rauenberg Datum' coordinate system. All map collar and inset information is also available as part of the raster image, including any inset maps, profiles, statistical tables, directories, text, illustrations, index maps, legends, or other information associated with the principal map. This map shows features such as roads, railroads and stations, drainage, built-up areas and selected buildings, fortification, ground cover, and more. Includes index to places of interest. This layer is part of a selection of digitally scanned and georeferenced historic maps from The Harvard Map Collection as part of the Imaging the Urban Environment project. Maps selected for this project represent major urban areas and cities of the world, at various time periods. These maps typically portray both natural and manmade features at a large scale. The selection represents a range of regions, originators, ground condition dates, scales, and purposes.