983 resultados para Laser Microdissection Microscopy


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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

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Pós-graduação em Ciências Odontológicas - FOAR

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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

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This study evaluated the effect on micro-tensile bond strength (mu-TBS) of laser irradiation of etched/unetched dentin through an uncured self-etching adhesive. Dentinal surfaces were treated with Clearfil SE Bond Adhesive (CSE) either according to the manufacturer's instructions (CSE) or without applying the primer (CSE/NP). The dentin was irradiated through the uncured adhesive, using an Nd: YAG laser at 0.75 or 1 W power settings. The adhesive was cured, composite crowns were built up, and the teeth were sectioned into beams (0.49 mm(2)) to be stressed under tension. Data were analyzed using one-way ANOVA and Tukey statistics (alpha = 5%). Dentin of the fractured specimens and the interfaces of untested beams were observed under scanning electron microscopy (SEM). The results showed that non-etched irradiated surfaces presented higher mu-TBS than etched and irradiated surfaces (p < 0.05). Laser irradiation alone did not lead to differences in mu-TBS (p > 0.05). SEM showed solidification globules on the surfaces of the specimens. The interfaces were similar on irradiated and non-irradiated surfaces. Laser irradiation of dentin through the uncured adhesive did not lead to higher mu-TBS when compared to the suggested manufacturer's technique. However, this treatment brought benefits when performed on unetched dentin, since bond strengths were higher when compared to etched dentin.

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The aim of this study was to evaluate the influence of chlorhexidine and Er, Cr:YSGG laser irradiation on the bond strength and external adaptation in mixed healthy and caries-affected class V cavities before and after thermal cycling. Thirty-six cavity preparations were made in mixed class V buccal human molars, half of them being artificially caries-induced. Any remaining affected dentin was removed from the cavity with a round burr at low speed. The teeth were divided into six groups, according to cleaning agent for both healthy and caries-induced dentin: no treatment, chlorhexidine and erbium, chromium-doped: yttrium, scandium, gallium, garnet (Er,Cr:YSGG) laser irradiation. A Filtek P90 (3M ESPE, St Paul, MN, USA) silorane adhesive restorative system was used. The specimens were subjected to 5000 thermal cycles (5-55 degrees C 60 min). Epoxy replicas were obtained to characterize the external adaptation under scanning electron microscopy. The average percentages of non-continuous margins were 5.41% and 6.49% in enamel dentin before thermal cycling and 25% and 33.7% after thermal cycling, respectively. The caries-affected and laser irradiated cavities showed higher non-continuous margins. Thermal cycling was able to raise the percentage of non-continuous margin for all groups. Chlorhexidine did not affect the marginal adaptation results, and the Er,Cr:YSGG laser irradiation showed significantly worse results compared with the control group.

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Experimental investigations were carried out using a Nd:YAG laser operating in pulsed mode for welding a lap joint between thin foil and thick sheet. The pulse energy was varied from 1.5 to 3.0 J at increments of 0.25 J with a 4 ms pulse duration. The base material used for this study was AISI 316L foils with 100 mu m thickness and sheet with 3.0 mm thickness. The welds were analysed by optical and electronic microscopy, tensile shear tests and micro hardness. The results indicate that pulse energy control is of considerable importance to join thin foil and thick sheet with good quality. The ultimate tensile strength of the welded joints increased at first and then decreased as the pulse energy increased. The process appeared to be very sensitive to the gap between couples. Large voids delimited by the molten zone boundary were observed in joints welded with high pulse energy.

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The aim of this study was to value the possibility to join, for pulsed Nd:YAG laser welding, thin foils lap joints for sealing components in corrosive environment. Experimental investigations were carried out using a pulsed neodymium: yttrium aluminum garnet laser weld to examine the influence of the pulse energy in the characteristics of the weld fillet. The pulse energy was varied from 1.0 to 2.5 J at increments of 0.25 J with a 4 ms pulse duration. The base materials used for this study were AISI 316L stainless steel and Ni-based alloys foils with 100 mu m thickness. The welds were analyzed by electronic and optical microscopy, tensile shear tests and micro hardness. The results indicate that pulse energy control is of considerable importance to thin foil weld quality because it can generate good mechanical properties and reduce discontinuities in weld joints. The ultimate tensile strength of the welded joints increased at first and then decreased as the pulse energy increased. In all the specimens, fracture occurred in the top foil heat-affected zone next to the fusion line. The microhardness was almost uniform across the parent metal, HAZ and weld metal. A slight increase in the fusion zone and heat-affected zone compared to those measured in the base metal was observed. This is related to the microstructural refinement in the fusion zone, induced by rapid cooling of the laser welding. The process appeared to be very sensitive to the gap between couples.

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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

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Objectives: the purpose of this study was to evaluate the surfaces of commercially pure titanium (cpTi) implants surface modified by laser beam (LS), by laser beam associated with sodium silicate deposition (SS) and compare them with surfaces modified by dual-acid etched (AS) and with machined surface (MS). Methods: thirty rabbits received two implants each (one for each tibia). After 30, 60 and 90 days postoperative, the implants were removed by reverse torque for biomechanical analysis and surfaces were analyzed by scanning electron microscopy (SEM) and X-ray energy dispersive spectroscopy (EDS). Results: the mean values of reverse torque at 30, 60 and 90 days postoperative were respectively 24.60, 43.60 e 60.40 N.cm to MS, 43.00, 68.20 e 63.80 N.cm to AS group, 59.80, 76.60 e 78.00 N.cm to LS group and 63.00, 75.40 e 76.60 N.cm to SS group. At 30 days, LS and SS groups showed statistically significant difference (p<0.05) compared to the other groups. At 60 days, LS and SS groups showed statistically significant difference (p<0.05) when compared to MS. Conclusions: it was concluded that SL and SS implants' biomechanical and topographical properties increased bone-implant interaction when compared to the AS and MS implants.