Losses of immunoreactive parvalbumin amacrine and immunoreactive alphaprotein kinase C bipolar cells caused by methylmercury chloride intoxication in the retina of the tropical fish Hoplias malabaricus

To quantify the effects of methylmercury (MeHg) on amacrine and on ON-bipolar cells in the retina, experiments were performed in MeHg-exposed groups of adult trahiras (Hoplias malabaricus) at two dose levels (2 and 6 µg/g, ip). The retinas of test and control groups were processed by mouse anti-parvalbumin and rabbit anti-_aprotein kinase C (_aPKC) immunocytochemistry. Morphology and soma location in the inner nuclear layer were used to identify immunoreactive parvalbumin (PV-IR) and _aPKC (_aPKC-IR) in wholemount preparations. Cell density, topography and isodensity maps were estimated using confocal images. PV-IR was detected in amacrine cells in the inner nuclear layer and in displaced amacrine cells from the ganglion cell layer, and _aPKC-IR was detected in ON-bipolar cells. The MeHg-treated group (6 µg/g) showed significant reduction of the ON-bipolar _aPKC-IR cell density (mean density = 1306 ± 393 cells/mm²) compared to control (1886 ± 892 cells/mm²; P < 0.001). The mean densities found for amacrine PV-IR cells in MeHg-treated retinas were 1040 ± 56 cells/mm² (2 µg/g) and 845 ± 82 cells/mm² (6 µg/g), also lower than control (1312 ± 31 cells/mm²; P < 0.05), differently from the data observed in displaced PV-IR amacrine cells. These results show that MeHg changed the PV-IR amacrine cell density in a dose-dependent way, and reduced the density of _aKC-IR bipolar cells at the dose of 6 µg/g. Further studies are needed to identify the physiological impact of these findings on visual function.

Avaliação da biometria testicular, concentração plasmática de hormônios e minerais em bovinos Nelore variedade Mocha dos 12 aos 24 meses de idade

Pós-graduação em Medicina Veterinária - FMVZ

Produção de biofilme em isolados de Candida spp do Hospital das Clínicas de Botucatu, UNESP

Pós-graduação em Biologia Geral e Aplicada - IBB

Effects of chronic stress and high-fat diet on metabolic and nutritional parameters in Wistar rats

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Prevalence and phenotypic characterization of Enterococcus spp. isolated from food in Brazil

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Vitamin D Induces Increased Systolic Arterial Pressure via Vascular Reactivity and Mechanical Properties

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Leuconostoc mesenteroides SJRP55: a potential probiotic strain isolated from Brazilian water buffalo mozzarella cheese

The probiotic potential of Leuconostoc mesenteroides subsp. mesenteroides SJRP55 isolated from water buffalo mozzarella cheese was evaluated. The microorganism presented resistance to stressful conditions that simulated the gastrointestinal tract, and to the best of our knowledge Leuconostoc mesenteroides SJRP55 was the first of this species with the ability to deconjugate bile salts. Tolerance to NaCl was temperature dependent, as well the results obtained by aggregation capacity. The strain presented good adhesion properties, β galactosidase activity, viability in fermented milk during storage, non-active against Streptococcus thermophilus and sensible to most of the tested antibiotics. Some analgesic medications inhibited the growth of the strain. Leuconostoc mesenteroides SJRP55 exhibited in vitro probiotic potential, and it can be better characterized through future in vivo tests. This bacterium presents higher functional properties compared to other studied strains, and therefore it is a potential candidate for the application as a probiotic strain, which could be used by industries in the manufacture of functional milk-based products.

Probiotic properties of lactic acid bacteria isolated from water-buffalo mozzarella cheese

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Leuconostoc mesenteroides SJRP55: a bacteriocinogenic strain isolated from Brazilian water buffalo mozzarella cheese

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Resposta imune inata em sistema nervoso central de camundongos experimentalmente infectados com amostras de vírus rábico isoladas de diferentes espécies

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Mudança social e cultural no Brasil nos últimos 50 anos: cenário e perspectivas para a melhoria do ensino público

Thinking about improving public education in Brazil means thinking not just what we wish to develop competencies and skills in individuals, but understands clearly identify the type of citizens who are training and what kind of society we want for the future. So if there are social and cultural changes they should have their comtraponto within the classroom considering education steeped in culture and therefore in the forms of modern communication. The challenge for school education is to show the students inside the classroom, as will be outside.

Cenário e perspectivas para a melhoria do ensino público no Brasil: mídia, cultura e educação

Thinking about improving public education in Brazil means thinking not only on what we wish to develop, skills and abilities in individuals, but understand and clearly identify the type of citizens we are devoloping and what kind of society we want for the future. In this context it is important to note that the practice in the classroom today can not be considered without regard to education steeped in culture and, therefore, in the forms of modern communication. So if there is a crisis in education, it can not be solved only within the classroom. The challenge for school education is to show students within the classroom how they will be out of school. Quality education is a blueprint for the future, to the unknown, to a world we do not know now. In this sense, to direct the education only for the labor market is a mistake, because the market is so today, but may be another in ten or fifteen years. Precisely because we do not know what are the requirements for citizens of the future, we must focus on what is essential in the formation of our youth. Is essential then to understand the ideas, learn to discuss them, argue and learn to recognize the role of technology in social and cultural evolution of mankind.

Fronteiras nas Américas: tamanho e composição dos domicílios rurais no oeste do Brasil e EUA na primeira metade do século XIX

The study of the size and composition of the rural household in tradicional societies in transition to modern ones is very useful as a tool in order to understand forms and organizations of the domestic groups and their possibilities of survival, social mobility e developing strategies of material accumulation. The aim of this article is to compare the size and composition of the rural households in the frontier regions of the Americas: the northwest and southwest of U.S.A with the southwest of São Paulo province in Brazil. The findings are surprising in comparative perspective, as the mean size of the American households were very high in relation to the brazilian ones. The sources used in this research are the manuscript census of the U.S.A and Brazil.